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1.
目的 评价超声洁牙术后使用护牙素和氟保护漆的联合干预措施促进牙釉质表面形态复原的作用.方法 收集因正畸拔除的健康前磨牙,采用简单随机方法分成4组.进行超声洁治后,分别不进行干预(无干预组),进行氟保护漆干预(氟保护漆组),涂布护牙素干预(护牙素组),用护牙素联合氟保护漆进行干预(氟保护漆+护牙素组).然后在不同时间段进行扫描电镜观察釉质表面形态,测定釉质表面粗糙度并进行外源性染色观察.结果 扫描电镜可观察到超声洁治后,各组牙釉质表面见深浅不一的划痕,干预后釉质表面超微结构都有改善,其中氟保护漆组、护牙素组和二者联合组改善较无干预组明显,而联合组改善最为明显;粗糙度测试显示,各组的粗糙度值随时间逐渐降低,其中干预组下降较明显,5d后,联合干预组较单独干预组的粗糙度值下降更加明显,差异有统计学意义(P<0.05);夕源性染色观察牙面着色,干预组比无干预组着色浅,在干预的3组中,护牙素联合氟保护漆组的术区着色最浅;牙面染色的定量分析可知,外源性染色前后比较,各干预组牙面的染色数值较染色前增高,差异有统计学意义(P<0.05),其中联合干预组较其他3组的染色的差异值最小.结论 护牙素联合氟保护漆比单独使用其中之一能更好地促进超声洁牙后牙釉质表面形态的复原,适宜在临床上推广应用. 相似文献
2.
目的:利用扫描电镜观察高功率微波(HPM)辐照后大鼠小肠形态结构的变化.方法:雄性SD大鼠35只随机分为对照组和试验组,在源距4.5m,场强100kV/m,总脉冲数40万次微波辐射后3,24,72h及7d活杀大鼠,取小肠组织;甲醛固定制备光镜标本;30mL/L戊二醛固定,进行常规扫描电镜标本制备.结果:扫描电镜观察对照组和HPM辐照后3h小肠形态基本正常,小肠绒毛排列整齐.辐照后24~72h,主要以肠黏膜破坏、肠上皮细胞水肿和分泌物增多为主.辐照后7d,分泌物减少,肠绒毛水肿减轻.结论:说明HPM辐照后扫描电镜观察大鼠小肠随作用时间的不同有不同程度的损伤. 相似文献
3.
肠易激综合征患者肠道屏障——紧密连接改变的示踪电镜观察 总被引:1,自引:0,他引:1
目的 观察肠易激综合征(IBS)患者肠道屏障-紧密连接的改变,探讨IBS排便性状改变的机制.方法 严格按照罗马Ⅱ标准选择IBS便秘型、腹泻型患者及正常对照组各10例,利用硝酸镧示踪电镜细胞化学技术,观察其回肠末端及升结肠粘膜紧密连接形态的变化.结果 10例便秘型IBS回肠末端粘膜及升结肠粘膜与正常对照组一样,紧密连接结构正常;10例腹泻型IBS中7例回肠末端粘膜及8例升结肠粘膜可见到部分细胞间紧密连接间隙增宽,示踪剂有不同程度的外渗现象.4例有感染性腹泻病史的患者中3例回肠末端及升结肠粘膜均有上述改变.结论 IBS患者的排便性状改变可能与紧密连接的结构和功能改变有关,紧密连接的开放性增强可能是导致腹泻性IBS腹泻的原因之一. 相似文献
4.
大肠癌“移行粘膜”病理变化研究——粘液组化、免疫组化及电镜观察 总被引:2,自引:0,他引:2
对200例大肠癌旁“移行粘膜”进行了粘液组化HID/AB及PAT/KOH/PAS检测,单克隆抗体MC3和CEA免疫组化标记,凝集素PNA、UEA-1及DBA受体测定,扫描电镜观察。其结果与10例正常直肠粘膜、130例癌组织、155例各型腺瘤型息肉及3-5个月胎儿大肠粘膜进行对照。结果表明大肠粘膜从良性到恶性的各阶段粘液组化及免疫组化的改变有显著差异(P<0.05或P<0.01),癌旁“移行粘膜”也存在类似癌及胎儿大肠的不成熟性病理变化,异常范围88.0%的病例在2-3cm以内。 相似文献
5.
补肾壮骨中药对模拟失重大鼠股骨远端松质骨的扫描电镜观察 总被引:2,自引:0,他引:2
目的 观察补肾壮骨中药对模拟失重状态下大鼠股骨远端松质骨超微结构的改变.方法 SPF级雄性SD大鼠15只.随机等分为3组:A组为自由活动对照组,B组和C组为尾吊模拟失重组,C组大鼠给予补肾壮骨中药灌胃,其他2组灌胃等量生理盐水,实验期28 d.利用扫描电镜对大鼠股骨远端松质骨的结构进行观察.结果 B组较A组大鼠的骨小梁变细,断裂,数量减少,微损伤增多;C组较B组大鼠骨小梁数量增加,骨吸收面减少,胶原纤维排列规律、整齐.结论 补肾壮骨中药能够对模拟失重状态下大鼠股骨远端松质骨的骨微结构产生有益影响. 相似文献
6.
目的 运用甘氨酰替甘氨酸浸泡法对常规的DMSO法冷冻-割断扫描电镜制样技术加以改良。方法 经过多聚甲醛戊二醛预固定的组织冷冻割断。1%OsO4后固定,用甘氨酰替甘氨酸溶液代替常规的低浓度锇酸液浸泡作细胞基质软化,后续步骤按常规方法,扫描电观察。结果 细胞内部膜系和丝状结构的超微形态保持完好,暴露明显,图像清晰,立体感强,各种膜系结构之间可见三维的丝状网络联系。结论 醛类预固定-甘氨酰替甘氨酸光地细 相似文献
7.
目的:探讨分肾功能(DRF)在成人单侧先天性肾盂输尿管连接部梗阻(UPJO)手术前后肾功能评价中的价值。方法选取2008年1月-2012年12月诊断为成人单侧先天性UPJO患者32例,术前利用肾动态显像测定分肾功能,将患者分为3组( DRF≥40%I组10例,30%≤DRF<40%II组14例,DRF<30%III组8例)。术后6个月再次复查并与手术前进行比较。结果术前患肾DRF≥40%(I组),术后患肾功能平均提高(2.18±3.25)%,与术前相比差异无显著性意义(P=0.463);术前患肾30%≤DRF<40%(II组),术后患肾DRF平均提高(11.37±3.82)%,与术前相比差异有显著性意义(P=0.000);DRF<30%(III组)术前术后平均DRF分别为(22.18±2.73)%和(24.35±3.56)%,差异无显著性意义(P>0.05)。结论 DRF能预测UPJO解除梗阻后肾功能的恢复情况。30%≤DRF<40%患者术后肾功明显改善,DRF≥40%与DRF<30%患者术后肾功能无明显改善。 相似文献
8.
以1,6-diphenyl-1,3,5-hexatrene(DPH)作为荧光探剂,应用荧光偏振技术研究帕金森氏病(PD)患者和正常对照组的红细胞膜微粘度,及TBA比色法测定血浆过氧化脂质(LPO),结果表明PD患者的红细胞膜微粘度及血浆LPO明显高于正常对照组,两指标间呈正相关关系。提示:检测红细胞膜流动性变化,可了解PD患者全身细胞受损害情况。 相似文献
9.
目的:探讨骨髓间质分离的多能成体祖细胞(MAPCs)移植后在大鼠脑组织内神经细胞分化的形态学观察。方法:制作帕金森病(PD)大鼠模型,将在体外纯化、增殖和用5-溴-2脱氧尿苷(BrdUrd)处理过的MAPCs注入PD大鼠脑内。3个月后,对移植大鼠采用免疫组化技术、免疫电镜等方法鉴定和分析MAPCs在大鼠脑组织内神经元样细胞分化的形态学。结果:MAPCs在中脑黑质和纹状体区分化为神经元样细胞和多巴胺能神经元;电镜下观察到MAPCs所分化的神经细胞与其他神经细胞形成突触联系。结论:骨髓间质分离的MAPCs移植后能在大鼠脑组织微环境中自主分化为多巴胺能神经细胞并有效地与其他神经细胞形成突触联系。因此MAPCs有望成为中枢神经系统疾病自体移植治疗的最佳候选干细胞之一。 相似文献
10.
大鼠局灶性脑缺血再灌注损伤动态病变及其与MMP-2表达关系 总被引:2,自引:0,他引:2
目的探讨大鼠局灶性脑缺血再灌注损伤的病变规律及MMP-2在损伤机制中的作用。方法建立大鼠大脑中动脉缺血再灌注模型,分别采用HE、TUNEL染色法观察缺血2 h再灌注3 h-7 d脑组织的动态病理变化及免疫组化法检测MMP-2的蛋白表达。结果假手术组大鼠脑组织形态结构正常;与之相比,缺血再灌注3 h-7 d组脑组织均有明显病变且差异有显著性(P<0.01);缺血中心区再灌注后3 h以变性损伤为主,24 h-7 d以坏死及继发炎症为主;边缘区一直以持续进行性凋亡为主;水肿贯穿病变全程,分别于再灌注后24 h、72 h两次出现高峰,其中24 h尚伴灶片状出血。缺血再灌注组大鼠大脑组织的MMP-2表达显著高于假手术对照组,P<0.05;MMP-2表达存在时空改变且与脑水肿、细胞凋亡有正相关性,P<0.01,相关度R值在0.5以上,其中24 h的首次脑水肿高峰与MMP-2表达峰值点重叠。结论再灌注后24 h、72 h的两次水肿高峰及迟发、持续性细胞凋亡是再灌注损伤两个最重要的方面,MMP -2表达参与了其机制。 相似文献
11.
Wei Liu Maria Luque Rudolf Glueckert Niklas Danckwardt-Lillieström Charlotta Kämpfe Nordström Anneliese Schrott-Fischer 《Upsala journal of medical sciences》2013,118(3):168-179
AbstractBackground: For the first time the expression of the ion transport protein sodium/potassium-ATPase and its isoforms was analyzed in the human cochlea using light- and confocal microscopy as well as super-resolution structured illumination microscopy. It may increase our understanding of its role in the propagation and processing of action potentials in the human auditory nerve and how electric nerve responses are elicited from auditory prostheses.Material and methods: Archival human cochlear sections were obtained from trans-cochlear surgeries. Antibodies against the Na/K-ATPase β1 isoform together with α1 and α3 were used for immunohistochemistry. An algorithm was applied to assess the expression in various domains.Results: Na/K ATPase β1 subunit was expressed, mostly combined with the α1 isoform. Neurons expressed the β1 subunit combined with α3, while satellite glial cells expressed the α1 isoform without recognized association with β1. Types I and II spiral ganglion neurons and efferent fibers expressed the Na/K-ATPase α3 subunit. Inner hair cells, nerve fibers underneath, and efferent and afferent fibers in the organ of Corti also expressed α1. The highest activity of Na/K-ATPase β1 was at the inner hair cell/nerve junction and spiral prominence.Conclusion: The human auditory nerve displays distinct morphologic features represented in its molecular expression. It was found that electric signals generated via hair cells may not go uninterrupted across the spiral ganglion, but are locally processed. This may be related to particular filtering properties in the human acoustic pathway. 相似文献
12.
冠心病患者介入治疗后血浆新型气体信号分子硫化氢及一氧化氮变化的临床研究 总被引:1,自引:0,他引:1
目的研究新型气体信号分子硫化氢(H2S)及一氧化氮(NO)在冠心病患者和冠脉造影正常者血浆中含量的差异及介入治疗对其的影响,探讨其在冠心病发病及介入治疗中的病理生理意义。方法冠心病组40例,造影正常组17例,采用硫敏感法测定术前血浆H2S含量并用Greiss法测定血浆中NO含量,动态监测冠心病患者冠状动脉造影前后、介入治疗后即刻、术后24h和72h血浆H2S、NO含量,分析冠心病组和造影正常组患者血浆H2S、NO含量的差异及介入治疗后血浆H2S和NO的变化。结果冠心病患者血浆H2S、NO含量远低于造影正常组(P均<0.01);冠脉双支和多支病变组血浆H2S含量差异无统计学意义(P>0.05),但均明显低于单支病变组(P<0.05和P<0.01)。不同支数病变冠心病患者血浆NO含量差异无统计学意义。冠脉血管有闭塞组其血浆H2S、NO含量明显低于单纯狭窄组(P均<0.05);支架植入术后复查狭窄者NO含量明显低于无狭窄者(P<0.05),H2S含量也低于无狭窄者,但差异无统计学意义(P>0.05)。冠状动脉造影术对血浆H2S、NO含量无影响,但PCI治疗术后即刻H2S、NO含量显著降低;H2S含量术后24h恢复至术前水平,NO含量术后24h降至最低水平,72h仍未恢复至术前水平。结论 H2S和NO可能参与了冠心病的发病过程及介入治疗后急性血管闭塞及再狭窄的发生,血浆H2S含量的高低与冠脉血管病变严重程度相关。 相似文献
13.
目的:探讨小细胞肺癌患者(SCLC)化疗前后血清CEA、NSE和CYFRA21-1变化在患者预后中的预测作用。方法:采用ELISA法检测63例病理确诊小细胞肺癌患者化疗前和两周期化疗后血清中CEA、NSE和CYFRA21-1的含量变化,化疗结束随访患者生存期。结果:全组患者平均生存时间为(21±4)月,中位生存时间为22月,1、2、3年生存率分别为76.2%、41.3%和14.3%。两周期化疗后CEA、NSE和CYFRA21-1水平降低的患者平均生存期分别为(25.7±3.5)月、(25.3±4.5)月、(24.5±5.3)月,明显较化疗后肺肿瘤标志物不降低患者平均生存期(16.0±3.9)月(P〈0.01)、(13.7±3.4)月(P〈0.01)、(16.7±3.2)月(P〈0.05)高,其1、2、3年生存率也有显著差异。结论:化疗后CEA、NSE和CYFRA21-1水平降低的患者生存期较不降低患者高,监测小细胞肺癌患者化疗前后肺肿瘤标志物的变化有助于评价患者对化疗药物敏感性及评估患者预后。 相似文献
14.
Yu-Heng Chen Zhang-Yan Lyu Gang Wang Xiao-Shuang Feng Shuang-Hua Xie Shuo-Hua Chen Jian Yin Jian-Song Ren Zi-Han Mi Shen Wang Shou-Ling Wu Ni Li Min Dai 《中华医学杂志(英文版)》2021,134(24):2976
Background:Prospective analyses have yet to identify a consistent relationship between sleep duration and the incidence of gastrointestinal (GI) cancers. The effect of changes in sleep duration on GI cancer incidence has scarcely been studied. Therefore, we aimed to examine the association between baseline sleep duration and annual changes in sleep duration and GI cancer risk in a large population-based cohort study.Methods:A total of 123,495 participants with baseline information and 83,511 participants with annual changes in sleep duration information were prospectively observed from 2006 to 2015 for cancer incidence. Cox proportional-hazards models were used to calculate hazard ratios (HRs) and their confidence intervals (CIs) for GI cancers according to sleep duration and annual changes in sleep duration.Results:In baseline sleep duration analyses, short sleep duration (≤5 h) was significantly associated with a lower risk of GI cancer in females (HR: 0.31, 95% CI: 0.10–0.90), and a linear relationship between baseline sleep duration and GI cancer was observed (P = 0.010), especially in males and in the >50-year-old group. In the annual changes in sleep duration analyses, with stable category (0 to −15 min/year) as the control group, decreased sleep duration (≤−15 min/year) was significantly associated with the development of GI cancer (HR: 1.29; 95% CI: 1.04–1.61), especially in the >50-year-old group (HR: 1.32; 95% CI: 1.01–1.71), and increased sleep duration (>0 min/year) was significantly associated with GI cancer in females (HR: 2.89; 95% CI: 1.14–7.30).Conclusions:Both sleep duration and annual changes in sleep duration were associated with the incidence of GI cancer. 相似文献
15.
Objective
To evaluate the effects of feet reflexology versus segmental massage in reducing pain and its intensity, frequency and duration of the attacks in females suffering from migraine.Methods
Forty eight females aged 33–58, suffering from migraine for 2 to 10 years were included in this study from November 2013 to November 2015. The study protocol was carried out in Department of Chronic Diseases at the Regional Hospital in Zywiec. In the reflexology group (RG) the patients received a series of 10 treatments 2 times per week; in the segmental massage group (SMG) the patients received a series of 15 treatments 3 times per week. Pain during migraine attacks was assessed using the visual analog scale (VAS), and headache features such as intensity (IA), frequency (FA) and duration (DA) of attacks were assessed before the treatment, just after the treatment, 3 months after the treatment.Results
All variables (VAS, IA, FA and DA) decreased within RG and SMG 3 months after the treatment in compare with the baseline values, and the differences were statistically significant. The differences between groups were also statistically significant.Conclusion
Feet reflexology and segmental massage provide a safe alternative for the pharmacological treatment of migraine. The patients with migraine obtain significant health benefits with feet reflexology. 相似文献16.
Kathrin Koucky Axel Wein Peter C. Konturek Heinz Albrecht Udo Reulbach Gudrun M?nnlein Kerstin Wolff Nicola Ostermeier Dagmar Busse Henriette Golcher Claus Schildberg Rolf Janka Werner Hohenberger Eckhart G. Hahn Jürgen Siebler Markus F. Neurath Frank Boxberger 《Medical science monitor》2011,17(5):CR248-CR258