食管癌组织中c-FLIP蛋白的表达及意义

采用免疫组化SP法检测64例食管癌组织和20例正常食管黏膜组织中的c-FLIP蛋白表达,分析其与食管癌临床病理特征和预后的关系。64例食管癌标本中51例c-FLIP蛋白呈阳性表达（79．69％）,明显高于正常食管膜组织（5％）,两者比较P〈0．01;c-FLIP蛋白表达与食管癌组织分化程度、TNM分期、淋巴结转移密切相关。c-FLIP蛋白低表达组的3a生存率明显高于高表达组（P〈0．01）。c-FLIP蛋白在食管癌组织中表达显著高于正常食管组织,其表达与食管癌的发生、发展、预后密切相关,可作为食管癌诊断、病情评估、预后判断的指标。     

CD147、EBP1 mRNA在新疆哈萨克族和汉族食管鳞癌中的表达及意义

目的探讨新疆维哈萨克族和汉族食管鳞状细胞癌中基因CD147和EBP1 mRNA的表达情况及其与临床病理特征之间的关系。方法采用RT-PCR技术检测CD147和EBP1 mRNA在37例新疆哈萨克族和40例汉族食管鳞癌组织及其对应的远隔正常食管组织中的表达。结果 CD147在食管癌组织和远隔正常食管组织中的相对表达量分别为0.883±0.040和0.675±0.023,P=0.013;EBP1分别为0.574±0.039和0.797±0.035,P=0.022。在汉族和哈萨克族食管鳞癌组织中CD147的阳性表达率分别为77.5%和81.0%,P=0.497;EBP1的阳性表达率分别为82.5%和78.4%,P=0.236。CD147在77例标本癌组织和远隔正常食管组织中的阳性表达率分别为79.2%和49.3%,P=0.003;EBP1在77例标本癌组织中的阳性表达率(61.0%)低于远隔正常食管组织(84.4%),P=0.005。CD147mRNA表达与浸润深度、淋巴结转移有关(P<0.05);EBP1 mRNA的表达与肿瘤分期及淋巴结转移相关(P<0.05)。CD147与EBP1的表达之间呈负相关。结论 CD147和EBP1 mRNA在两民族食管鳞癌中的表达无差异;CD147高表达和EBP1低表达可能共同促进食管鳞癌的发生、发展。     

食管鳞癌组织中B7-H4、CD3表达变化及意义

目的探讨B7-H4、CD3在食管鳞癌发生、发展中的作用。方法采用免疫组化SP法检测30例食管鳞癌组织（食管癌组）和20例癌旁正常食管黏膜组织（对照组）中B7-H4、CD3蛋白表达,采用等级相关方法分析B7-H4和CD3表达的相关性。结果食管癌组B7-H4阳性表达率高于对照组,CD3阳性表达率低于对照组（P均〈0.05）。不同临床分期及淋巴结转移情况者食管癌组织中B7-H4表达差异有统计学意义（P均〈0.05）。食管癌组织中B7-H4与CD3蛋白的表达呈负相关关系（r=-0.467,P〈0.05）。结论食管鳞癌组织中B7-H4呈高表达,CD3呈低表达,二者与食管鳞癌的发生发展及侵袭转移有关。     

食管癌中桩蛋白、CD44V6的表达及意义

目的探讨桩蛋白（Paxillin）和CD44V6在食管癌组织中的表达及其与食管癌生物学行为的关系。方法采用免疫组化SP法检测食管癌组织中Paxillin和CD44V6的表达及两者的相关性。结果Paxillin和CD44V6在食管鳞癌和腺癌中的表达高于食管正常黏膜（P〈0．01）;Paxillin和CD44V6在食管鳞癌中表达与临床分期（P〈0．01）、浸润深度（P〈0．01）和淋巴结转移（P〈0．05）有关;Paxillin和CD44V6在食管鳞癌中表达无相关性（P〉0．05）。结论Paxillin和CD44V6的表达在食管鳞癌的侵袭转移过程中发挥着重要的作用,可作为反映食管癌肿瘤生物学指标和抗肿瘤治疗的靶点。     

食管癌组织中ABCG2的表达变化及意义

目的观察ABCG2在食管癌组织中的表达变化,并探讨其意义。方法收集80例食管癌患者的癌组织及癌旁正常食管组织,进行ABCG2的免疫组化染色及Western blot检测。结果 80例食管癌患者,其癌组织中ABCG2的表达较正常食管组织明显上调（P〈0.05）。患者的3年生存率与癌组织中ABCG2的表达量有关（P〈0.01）,癌组织中ABCG2的表达上调与术后早期复发密切相关（P〈0.05）。结论 ABCG2在食管癌组织中的表达上调,可以作为食管癌患者预后的预测因子之一。     

胆管细胞癌细胞CD147表达及其临床意义*

目的 探讨CD147在肝胆管结石并发胆管细胞癌（ICC）组织的表达及其临床意义。方法 收集肝胆管结石并发ICC标本30例、对应的癌旁肝组织标本30例和正常肝标本10例。采用qRT-PCR法检测组织CD147 mRNA水平,采用免疫组化法检测CD147蛋白的表达;采用Western-Blotting法检测基质金属蛋白酶-2（MMP-2）和9（MMP-9）蛋白的表达;采用肯德尔（Kendall）等级相关分析CD147 mRNA、CD147、MMP-2和MMP-9表达的相关性。结果 经qRT-PCR检测显示,肝胆管结石并发ICC组织CD147mRNA水平为（3.67±1.88）,显著高于癌旁组织【（1.52±0.57）,P<0.01】和正常肝组织【（1.05±0.32）,P<0.01】,癌旁组织高于正常肝组织（P<0.01）;>5 cm ICC组织CD147 mRNA相对水平（2.73±0.97）显著高于<5 cm肿瘤组织【（1.03±0.32）,P<0.01】,有淋巴转移（2.68±0.74）组织显著高于无转移组织【（1.07±0.44）,P<0.01】,肿瘤分化程度低组织（2.71±0.86）显著高于中高分化组织【（1.06±0.42）,P<0.01】;不同年龄和性别患者ICC组织CD147 mRNA水平无显著性差异（P>0.05);经免疫组化检测显示,肝胆管结石并发ICC组织CD147蛋白表达相对水平为（27.95±4.90）,显著高于癌旁组织【（13.34±9.59）,P<0.01】和正常肝组织【（2.18±0.66）,P<0.01】,而癌旁组织高于正常肝组织（P<0.01）;ICC组织MMP2表达量为（1.06±0.13）,显著高于癌旁组织【（0.20±0.03）,P<0.01】和正常肝组织【（0.15±0.02）,P<0.01】,而癌旁组织MMP2表达量显著高于正常肝组织（P<0.01）;肝胆管结石并发ICC组织MMP9表达量为（0.93±0.12）,显著高于癌旁组织【（0.17±0.03）,P<0.01】和正常肝组织【（0.15±0.03）,P<0.01】,而癌旁组织MMP9表达量显著高于正常肝组织（P<0.01）;在ICC组织中,CD147mRNA水平与MMP2蛋白表达呈正相关（r=0.457,P<0.05）;CD147mRNA水平与MMP9蛋白表达也呈正相关（r=0.428,P<0.05）;CD147蛋白与MMP2蛋白表达呈正相关（r=0.543,P<0.05）,CD147蛋白与MMP9蛋白表达也呈正相关（r=0.517,P=0.05）。结论 CD147 mRNA、CD147、MMP2、MMP9均在肝胆管结石并发ICC组织中表达增强,CD147的表达可能促进了MMP2和MMP9的表达上调,它们可能共同参与了胆管癌的发生和发展过程。     

食管癌组织中CD44v6基因转录表达意义

目的探讨CD44v6 mRNA及其蛋白表达与食管癌病理生物学行为的关系,为观测食管癌侵袭转移潜能和评估胃癌患者预后寻求一个新的客观生物学指标.方法应用CSA法原位杂交和免疫组化技术,检测65例食管癌组织中CD44v6 mRNA及其蛋白表达,并结合肿瘤的病理生物学行为和临床随访资料分析.结果在食管癌中CD44v6 mRNA和其蛋白表达阳性率分别为61.5%(40/65)和58.5%(38/65),其表达均与食管癌浸润,淋巴结转移和预后密切相关(P＜0.05).结论检测CD44v6表达可作为预测食管癌转移潜能和评估患者预后的一个新的生物学指标.     

FAK在食管癌组织中的表达及临床意义

目的检测局部黏着斑激酶(focal adhesion kinase,FAK)在食管癌组织及正常食管组织中的表达,探讨FAK在食管癌发生、发展中的意义。方法采用免疫组化SP法检测FAK在40例食管癌组织、40例正常食管组织中的表达情况。结果免疫组化结果提示:40例食管癌组织中有16例(40.0%)FAK呈强阳性染色,15例(37.5%)FAK呈弱阳性染色,9例(22.5%)FAK表达缺失;40例正常食管组织中,3例(7.5%)FAK呈强阳性染色,7例(17.5%)FAK呈弱阳性染色,30例(75.0%)FAK表达缺失,FAK在食管癌组织的阳性表达率(77.5%)显著高于正常食管组织的阳性表达率(25.0%),两组比较差异有统计学意义(P0.05)。FAK的表达与食管癌患者的性别、年龄无关(P0.05),而与分化程度相关(P0.05),低分化食管癌组FAK蛋白表达高于高中分化组。结论 FAK在食管癌组织中表达增高,其表达量与食管癌的分化程度有关,FAK在正常食管组织中表达缺失或减少。FAK可能与食管癌的发生、发展有重要关系,其表达的高低有可能作为食管癌预后判断的一种新指标。     

食管鳞癌组织中STAT3蛋白的表达及临床意义

目的 探讨食管鳞癌组织和癌旁正常黏膜组织中信号转导子和转录激活子3（STAT3）的表达及与食管鳞癌发生发展的关系.方法 应用免疫组化SP法检测122例食管鳞癌及其癌旁正常黏膜组织中STAT3蛋白的表达.结果 食管鳞癌组织中STAT3蛋白表达阳性率为89.3%,明显高于癌旁正常黏膜组织的77%（P＜0.05）.Ⅰ级、Ⅱ级、Ⅲ级食管鳞癌组织中STAT3蛋白的阳性率分别为73.7%、89.5%和100%,Ⅲ级中STAT3蛋白的阳性率显著高于Ⅰ级（P＜0.05）.浸润至深层（深肌层和外膜）的食管鳞癌组织中STAT3蛋白阳性表达率为92.8%,明显高于浸润至浅层（黏膜和浅肌层）食管鳞癌组织的76%（P＜0.05）.STAT3的表达与淋巴结转移无关（P＞0.05）.结论 STAT3蛋白过度表达与食管鳞癌的发生发展及恶性演进有关,STAT3有望成为评估食管癌预后的一个新标志物.     

凋亡相关基因在食管上皮癌变中的表达及其意义

目的研究细胞凋亡调控因子Fas、Fas配体（Fasl）、Fas相关的死亡结构蛋白（Fadd）和半胱氨酸蛋白酶（Caspase8）基因在食管上皮癌变过程中的表达及其意义。方法应用免疫组化S-P法检测60例食管癌组织，30例不典型增生组织及30例正常食管黏膜中Fas、Fasl、Fadd Caspase8的蛋白表达。应用原位杂交法检测60例食管癌组织，30例不典型增生组织及30例正常食管黏膜中Caspase8 mRNA的表达。结果从食管正常黏膜组织到不典型增生组织和食管癌组织，Fas蛋白表达率呈逐渐下降的趋势，Fasl蛋白表达率呈逐渐上升的趋势，两者在食管癌组织和正常组织之间有显著性差异（P〈0．05）。高中分化鳞癌中蛋白阳性表达率显著高于低分化鳞癌（P〈0．05）。从食管正常黏膜组织到不典型增生组织和食管癌组织，Fedd和caspase8蛋白与Caspapac8 mRNA阳性表达率呈逐渐下降的趋势，在食管癌与正常黏膜组织之间有显著性差异（P〈0．01或P〈0.05）。Caspac8蛋白与mRNA高中分化鳞癌表达率高于低分化鳞癌（P〈0．05）。结论Fas与Fasl的表达失衡与食管癌的发生、发展有密切关系，并与食管癌的分化和免疫逃避有关。Fadd与Caspase8基因表达异常在食管癌的发生发展中可能起着重要作用。     

Clinical Impact of HAb18G/CD147 Expression in Esophageal Squamous Cell Carcinoma

Background

HAb18G/CD147 expression has been associated with many tumor invasion molecules, which play important roles in recurrence and poor differentiation of esophageal squamous cell carcinoma (ESCC). However, the clinical implications of HAb18G/CD147 in ESCC are still unclear.

Aims

In this study, we clarified the clinical significance of HAb18G/CD147 and characterized the association between HAb18G/CD147 and tumor invasion in ESCC cases.

Methods

Tumor tissues were obtained from 86 ESCC patients who underwent surgical resection between 2002 and 2005. All patients that had received previous therapy were excluded. ESCC tissues were analyzed by IHC using anti HAb18G/CD147 antibody. The expression of HAb18G/CD147 mRNA in esophageal cancer cell lines was analyzed by RT?CPCR.

Results

HAb18G/CD147 was uniformly expressed in EC109 and EC871214 cell lines, but negatively expressed in EPC2, esophageal normal squamous cell line. HAb18G/CD147 mainly localized to the membrane of tumor cells in 84.9% of ESCC patients (64 out of 86 cases). Furthermore, we also found that higher HAb18G/CD147 expression levels significantly correlated with lymph node metastasis, depth of tumor invasion and differentiation (P < 0.05). But the expression levels of HAb18G/CD147 in lymph node metastatic tissues were almost equal to that in the primary tumor tissues. Furthermore, lymph node metastasis and expression of HAB18G/CD147 were independent prognostic indicators in ESCC.

Conclusions

The expression of HAb18G/CD147 might be involved in the progression and survival of ESCC. Therefore, HAb18G/CD147 could be a clinical marker for the poor prognosis in ESCC patients and may also be a potentially therapeutic target to improve the progression of ESCC.     

Expression of CD147 in advanced non-small cell lung cancer correlated with cisplatin-based chemotherapy resistance

CD147, a widely expressed cell surface glycoprotein in cancer, is associated with tumor invasiveness and chemotherapy resistance. Recently, CD147 is also regarded as a potential therapeutic target for cancer therapy. The aim of the study was to investigate CD147 expression in non-small cell lung cancer (NSCLC), and evaluate its correlation with cisplatin-based chemotherapy resistance. In this study, we examined immunohistochemically the expression of CD147 in 118 advanced NSCLC cases treated with cisplatin-based chemotherapy, and then the association of CD147 expression with clinicopathological characteristics was analyzed. Furthermore, RNA interference approach was used to silence CD147 expression in a cisplatin-resistant human lung cancer cell line A549/DDP, and the inhibition effect of cisplatin on tumor cells was assayed by MTT. In the overall series, positive CD147 expression was observed in 101/118 (85.6%) cases. A membranous CD147 pattern was identified in 76/101 (75.2%) of CD147 positive tumors. CD147 membranous expression,but not the overall CD147 expression, was associated with poor response to cisplatin-based chemotherapies and a poor prognosis in advanced NSCLC patients. In vitro results showed that silencing CD147 increased the proliferation inhibitory effect of cisplatin to A549/DDP cells. In conclusion, our study indicated that membranous CD147 expression is a predictive factor of the response to cisplatin-based chemotherapies, and the use of CD147-targeted therapeutic adjuvants might be considered in the treatment of advanced NSCLC patients.     

24-乙酰泽泻醇A对氧化型低密度脂蛋白诱导巨噬细胞脂代谢因子ABCA1、CD36及炎症因子CD147、MMP-9的影响

目的通过体外实验探讨24-乙酰泽泻醇A对氧化型低密度脂蛋白(ox-LDL)诱导大鼠腹腔巨噬细胞脂代谢因子ATP结合盒转运体A1(ABCA1)、B族清道夫受体(CD36)和炎症因子细胞外基质金属蛋白酶诱导因子(CD147)、基质金属蛋白酶9(MMP-9)蛋白表达的影响。方法分别采用50 mg/L ox-LDL和10 mg/L Dil-ox-LDL诱导巨噬细胞,10 mg/L 24-乙酰泽泻醇A进行干预。荧光显微镜观察细胞内Dil-ox-LDL蓄积情况;蛋白免疫印迹检测细胞ABCA1、CD36、CD147、MMP-9蛋白的表达。结果 10 mg/L Dil-ox-LDL诱导后大鼠腹腔巨噬细胞内有大量的Dil-ox-LDL蓄积,10 mg/L 24-乙酰泽泻醇A干预后,细胞内Dil-ox-LDL蓄积明显减轻。与对照组比较,50 mg/L ox-LDL诱导后大鼠腹腔巨噬细胞ABCA1、CD36和CD147、MMP-9蛋白表达明显增加,10 mg/L 24-乙酰泽泻醇A干预后,ABCA1蛋白表达进一步上升(P0.01),CD36、CD147和MMP-9蛋白表达被明显抑制(P0.05或P0.01)。结论 24-乙酰泽泻醇A上调巨噬细胞的脂代谢因子ABCA1和抑制CD36的表达,减少胆固醇蓄积,同时抑制炎症因子CD147和MMP-9的分泌。     

CD147和基质金属蛋白酶-2在大肠癌组织中的表达及其临床意义

目的 研究细胞外基质金属蛋白酶诱导因子(CD147)和基质金属蛋白酶-2(MMP-2)在大肠癌组织中的表达及其与大肠癌临床病理因素间的关系,探讨其与大肠癌侵袭和转移的相关性及二者间的相互联系.方法 应用免疫组织化学方法检测44例大肠癌患者癌组织、癌旁组织和正常对照组织中CD147和MMP-2的表达,以图象分析软件进行半定量分析.结果 大肠癌组织、癌旁组织和正常对照组织中CD147和MMP-2表达阳性率呈递减改变.CD147和MMP-2的表达部位和染色强度相似,半定量分析显示两者呈正相关(r=0.777,P＜0.05).两者的表达与大肠癌患者年龄、肿瘤分型、肿瘤分化程度未见相关性(P＜0.05),而在大肠癌患者伴转移组显著高于无转移组(P＜0.05).结论 CD147在大肠癌患者组织中的表达增高与大肠癌侵袭转移密切相关,其促MMP-2的激活和表达增高的作用可能是其中一个重要因素.     

食管癌中细胞粘附分子E-钙粘素和CD44变异体表达及其临床意义

目的　探讨细胞粘附分子E 钙粘素 (E CD)和CD44变异体 (CD44V6)的表达与食管癌临床病理及预后的关系。方法　对 5 8例食管癌标本应用SP免疫组化染色法进行E CD、CD44V 6检测 ,并对其中 42例进行术后 3年随访。结果　E CD低表达和CD44V 6高表达与食管癌的浸润转移、复发和预后相关 (P <0 .0 5 )。E CD低阳性表达率与食管癌细胞分化程度相关 (P <0 .0 5 )。食管癌中E CD和CD44V6的表达无相关性 (P >0 .0 5 )。结论　E CD和CD44V 6在食管癌中的表达与食管癌的发生发展、侵袭转移和预后密切相关 ,E CD和CD44V6在食管癌中的表达无相关性 ,在食管癌的发生、发展、侵袭转移中是 2个独立的调节因子 ,可视为预测侵袭转移和估计预后的重要参考指标     

Correlation of matrix metalloproteinase suppressor genes RECK, VEGF, and CD105 with angiogenesis and biological behavior in esophageal squamous cell carcinoma

AIM： To explore the expression of reversion inducing cysteine-rich protein with Kazal motifs （RECK）, vascular endothelial growth factor （VEGF） and endoglin （CD105） protein and its correlation with occurrence, development, invasion and metastasis in esophageal squamous cell carcinoma （ESCC）. METHODS： Streptavidin-peroxidase （SP） immunohistochemistry was used to detect expression of RECK and VEGF in 62 cases of ESCC, 31 cases of adjacent atypical hyperplastic epithelium and 62 cases of normal esophageal epithelium. CD105 Mb was used to assess microvessel density （MVD）. RESULTS： The expression of RECK was closely correlated with histological grade, infiltrative depth and lymphatic metastasis in ESCC （P 〈 0.05）. The expression of RECK decreased during cancer development： normal esophageal epithelium （85.5%, 53/62）, adjacent atypical hyperplastic epithelium （71.0%, 22/31）, and carcinoma （59.7%, 37/62）. There was a significant difference among the groups （P 〈 0.05）. The expression of VEGF protein was closely correlated with infiltrative depth and lymphatic metastasis in ESCC （P 〈 0.05）. The expression of VEGF protein increased during cancer development： normal esophageal epithelium （29.0%, 18/62）, adjacent atypical hyperplastic epithelium （54.8%, 17/31）, and carcinoma （67.7%, 42/62）. There was a significant difference among the groups （P 〈 0.05）. MVDCD105 increased in accordance with histological grade, butthere was no significant difference （grade Ⅰ, 36.92 ± 10.85; grade Ⅱ, 37.65 ± 9.50; and grade Ⅲ, 38.06 ± 12.19）. The MVDCD105 was closely correlated with infiltration and lymphatic metastasis in ESCC （P 〈 0.05）. The expression of RECK was inversely correlated with the expression of VEGF and CD105.CONCLUSION： RECK, VEGF and CD105 play important roles in the infiltration, metastasis and carcinogenesis in esophageal carcinoma. Angiogenesis in ESCC may be promoted by over-expression of CD105.     

Complement regulatory proteins in normal human esophagus and esophageal squamous cell carcinoma

BACKGROUND: Altered expression of three complement regulatory proteins, decay-accelerating factor (CD55), membrane cofactor protein (CD46) and homologous restriction factor 20 (CD59) has been identified in human gastrointestinal malignancies, but their expression in esophageal cancer has not been described. Therefore the purpose of the present paper was to study the distribution of these proteins in human normal and malignant esophageal mucosa. METHODS AND RESULTS: In the normal esophageal mucosa, CD55 predominantly stained on the cell membrane of squamous epithelium in the superficial and prickle cell layers, whereas CD46 most intensely stained on the cell membrane in the basal and parabasal cell layers. In contrast to this reciprocal expression of CD55 and CD46, CD59 was broadly distributed on the cell membrane in all layers. In the esophageal squamous cell carcinoma, CD55 staining was intense in the stroma but was negligible in the cancer cells. In contrast, CD46 and CD59 stained almost uniformly on the tumor cell membrane. There was a significant difference in the intensity of the staining of CD55 and CD46 among cells in various layers of normal esophageal mucosa and esophageal carcinoma cells, but not in the staining of CD59. Similar expression patterns of the three complement regulatory proteins in carcinoma cells and in normal epithelium in the basal and parabasal cell layers were observed. CONCLUSIONS: These observations on the expression of the three complement regulatory proteins would help understanding of the host immune responses involving the complement system against esophageal squamous cell carcinoma.     

癌组织及外周血中CD44、PLCEl、甲基化Sept9基因及DNA错配修复蛋白表达水平与结直肠癌病理分期及预后的相关性分析

目的探讨癌组织及外周血中白细胞分化抗原分化簇第44号(CD44)、磷酯酶Cεl(PLCEl)、甲基化Sept9基因及DNA错配修复蛋白表达水平与结直肠癌病理分期及预后的相关性。方法将2013年3月至2015年5月在西宁市第二人民医院确诊为结直肠癌的56例患者设为观察组,将同期于我院体检的55名健康成年人设为对照组。比较两组甲基化Sept9基因、CD44、PLCEl及DNA错配修复蛋白表达情况,分析以上指标在结直肠癌患者中的临床分布特点并进行相关性检验,比较不同甲基化Sept9基因、CD44、PLCEl及DNA错配修复蛋白表达情况并分析其与结直肠癌患者的预后相关性。结果观察组甲基化Sept9基因及CD44表达阳性率、PLCEl表达阴性率、DNA错配修复蛋白表达缺失率高于对照组(P<0.05)。甲基化Sept9基因、PLCEl及DNA错配修复蛋白在不同肿瘤浸润深度、肿瘤大小、病理分期及有无淋巴结转移结直肠癌患者中的表达差异有统计学意义(P<0.05)。CD44在不同肿瘤浸润深度、病理分期及有无淋巴结转移结直肠癌患者中的表达差异有统计学意义(P<0.05)。PLCEl与结直肠癌的浸润深度、病理分期呈负相关(r=-0.367,P=0.045;r=-0.522,P=0.008);甲基化Sept9基因与浸润深度、病理分期、淋巴结转移呈正相关(r=0.715,P=0.026;r=0.471,P=0.032;r=0.453,P=0.010),CD44与浸润深度、病理分期、淋巴结转移呈正相关(r=0.349,P=0.007;r=0.591,P=0.022;r=0.452,P=0.027),DNA错配修复蛋白与病理分期﹑淋巴结转移呈负相关(r=-0.487,P=0.041;r=-0.551,P=0.030)。不同CD44、甲基化Sept9基因、PLCEl及DNA错配修复蛋白表达情况的结直肠癌患者3年生存率差异有统计学意义(P<0.05)。PLCEl、DNA错配修复蛋白与结直肠癌患者3年生存率呈正相关(r=0.574,P=0.041;r=0.478,P=0.037),甲基化Sept9基因与结直肠癌患者3年生存率呈负相关(r=-0.515,P=0.034)。结论CD44、PLCEl、甲基化Sept9基因及DNA错配修复蛋白均与结直肠癌的病理分期相关,其检测有助于了解疾病的恶性程度,评估患者预后。     

Expression of human chorionic gonadotropin, CD44v6 and CD44v4/5 in esophageal squamous cell carcinoma

AIM: To study the relationship between the expression of human chorionic gonadotropin (HCG), CD44v6, CD44v4/5 and the infiltration, metastasis of esophageal squamous cell carcinoma. METHODS: By labeled streptavidin-biotin technique, the expressions of HCG, CD44v6, and CD44v4/5 in 42 patients with esophageal squamous cell carcinoma were examined. RESULTS: The positive rate of HCG expression in patients with lymph node metastasis was 85.71% (18/21), higher than that (57.14%, 12/21) in those without lymph node metastasis (P<0.05). The positive rate of CD44v6 expression was 71.43% (15/21) in lymph node metastasis group, and 38.09% (8/21) in non-metastasis group; there was a significant difference between the two groups (P<0.05). The positive rate of CD44v4/5 expression was 76.19% (16/21) in lymph node metastasis group, and 42.86% (9/21) in non-metastasis group; there was also a significant difference between them (P<0.05). From grade Ⅰ to grade Ⅲ in differentiation, the positive rate of HCG expression was 84.62% (11/13), 70.59% (12/17) and 58.33% (7/12), respectively; there was no significant difference among them (P<0.05). The positive rate of CD44v6 expression in grades Ⅰ-Ⅲ of cancer tissues was 76.92% (10/13), 52.94% (9/17), and 33.33% (4/12) respectively; there was no significant difference among them. The positive rate of CD44v4/5 expression in grades Ⅰ-Ⅲ of cancer tissues was 69.23% (9/13), 64.71% (11/17), and 41.67% (5/12) respectively; there was no significant difference among the three groups. There was no correlation between the positive rates of HCG and CD44v6, CD44v4/5 expression. Cancer cells in carcinomatous emboli and those infiltrating into vascular wall strongly expressed HCG, CD44v6, and CD44v4/5. CONCLUSION: Expression of HCG, CD44v6, and CD44v4/5 in esophageal squamous cell carcinoma is related to its infiltration and metastasis. HCG, CD44v6, and CD44v4/5 have different effects on the infiltration and metastasis of esophageal squamous cell carcinoma.     

食管癌核基质抗体的免疫组化研究

目的研究食管癌核基质抗体的肿瘤特异性和组织学特异性．方法用人食管癌组织提取核基质抗原，制备核基质抗体，采用免疫组织化学方法对食管癌４１例（男２６例，女１５例，年龄５３岁±７２岁）、正常食管粘膜８例、食管粘膜异型增生１５例、肺鳞癌１０例、喉癌１０例、胃腺癌１０例以及大鼠食管癌５例进行免疫组化染色．结果食管癌核基质抗体在食管癌组织中表达有特异性（３２／４１，７８０％），与正常食管粘膜（１／８，１２５％）及胃腺癌（２／１０，２００％）有非常显著差异（Ｐ＜００１），与食管异常增生（７／１５，４６７％）、肺鳞癌（３／１０，３００％）、喉鳞癌（４／１０，４００％）差异明显（Ｐ＜００５），但与大鼠食管癌组织（２／５，４００％）差异不明显．食管癌核基质的表达，在不同分化程度的食管癌组织中无明显差异（Ｐ＞００５）；在淋巴结转移阳性组高于淋巴结转移阴性组（１７／１８，９４４％ｖｓ１５／２３，６５２％，Ｐ＜００５）．结论食管癌核基质抗体具有较好肿瘤和组织学特异性，对肿瘤转移有一定影响，可作为食管癌的一项新标记物．