唾液腺腺样囊性癌中TRPM的表达及意义

目的检测唾液腺腺样囊性癌(ACC)中瞬时受体势M(TRPM)的表达及分布情况的变化,以探究ACC的发病机理。方法采用免疫印迹和免疫组织化学方法,检测TRPM在培养的ACC-2细胞及ACC组织中的表达,采用方差分析,利用OriginPro 7.0软件进行统计学分析。结果ACC-2细胞和ACC组织中TRPM7蛋白的表达水平明显高于正常组织。TRPM7蛋白表达量经灰度值分析,正常腮腺组织为0.42±0.044,多形性腺瘤组织为0.413±0.085,而腺样囊性癌组织为0.85±0.045,差异有显著性意义(P<0.001)。结论ACC中TRPM表达水平的增高可能是ACC发病的组织病理学基础。     

Survivin在腺样囊性癌细胞ACC-2以及ACC-M中的表达

目的：探讨Survivin在腺样囊性癌（ACC）细胞系中的表达情况及其表达与ACC远处转移的相关性。方法：培养人涎腺腺样囊性癌（ACC-2）和肺转移腺样囊性癌（ACC-M）细胞,观察其形态学特征,采用MTr法检测细胞的生长活性,并采用SP法检测细胞中Survivin的表达。结果：ACC-2和ACC-M细胞均表现有明显的上皮样和腺样细胞的特征。ACC-M细胞的生长活性高于ACC-2细胞,且Survivin在ACC-M细胞中的表达也强于ACC-2细胞。结论：Survivin与涎腺ACC肺部转移的发生具有高度相关性。     

转化生长因子β1对口腔鳞癌及腺样囊性癌细胞纤维黏连蛋白基因EDA片段调节作用的探讨

目的探讨转化生长因子β1（transforming growth factor-β1, TGF-β1）对口腔鳞癌及腺样囊性癌细胞中纤维黏连蛋白（fibronectin，FN）基因EDA片段的调节作用。方法应用免疫组织化学及半定量逆转录聚合酶链反应法（RT-PCR），分别从蛋白及mRNA水平观察口腔鳞癌及腺样囊性癌高、低转移潜能细胞中加入外源性TGF-β1后FN基因EDA片段的表达变化情况。结果3株细胞加入TGF-β1组与未加TGF-β1组EDA片段蛋白表达阳性细胞率比较显示，口腔鳞癌细胞株（Tca83）阳性细胞率由15．4±4．1增加到50．7±10．5，差异有统计学意义（P〈0．01），腺样囊性癌低转移株（SACC-83）阳性细胞率由71．9±4．8增加到86．1±5．5，差异也有统计学意义（P〈0．05），但腺样囊性癌高转移株（SACC-LM）阳性细胞率由93．3±2．4增加到94．1±3．4，差异无统计学意义（P〉0．05）。3株细胞加入TGF-β1组EDA^＋ mRNA表达较未加TGF-B1组明显升高，而EDA-mRNA的表达下降。且差异均有统计学意义（P〈0．05）。结论高浓度TGF-β1可以影响口腔鳞癌及腺样囊性癌细胞中FN基因EDA片段的剪切并促进EDA片段的表达，可能成为影响肿瘤细胞黏附能力及肿瘤侵袭和转移的相关因素。     

基质金属蛋白酶-1在人涎腺腺样囊性癌细胞中的表达

目的:测定基质金属蛋白酶-1(MMP-1)在人涎腺腺样囊性癌不同转移力细胞系中的表达。方法:以人涎腺腺样囊性癌ACC-2细胞系及其高转移株ACC-M作为研究肿瘤转移分子机制的模型,采用免疫组化法和蛋白印迹(Western blot)法检测MMP-1的蛋白表达水平。结果:MMP-1在ACC-M细胞中的表达水平高于ACC-2细胞。结论:MMP-1在人涎腺腺样囊性癌的侵袭转移过程中可能发挥作用。     

TGF-β1在口腔颌面部恶性肿瘤中的表达

目的 探讨转化生长因子β1（TGF－β1）在人口腔颌面部恶性肿瘤细胞中的表达水平与其生物学行为的关系。方法 以ELISA法分别检测人口腔颌面部手术切除恶性肿瘤组织细胞，人舌鳞状细胞癌细胞系Tca8113，人涎腺腺样囊性癌细胞系ACC－2和人高转移性涎腺腺样囊性癌细胞系ACC－M，及正常口腔黏膜与黏膜下组织细胞各样本的TGF－β1的表达水平。结果 口腔合面部恶性肿瘤组织TGF－β1的表达水平与正常对照组有非常显著差异（P＜0．01）。Tca8113,ACC-2细胞TGF－β1的表达水平增高正常对照组细胞（P＜0．05）；舌鳞癌系Tca8113与腺要囊性癌细胞系ACC－2的TGF－β1的表达水平无显著差异；高转移腺样囊性癌细胞系ACC－M的TGF－β1的表达水平与ACC－2相比，有非常显著的降低（P＜0．01）；高转移腺样囊性癌细胞系ACC－M无血清培养液中TGF－β1的分泌量亦低于任何其它细胞。结论 TGF－β1的表达水平可能与恶性肿瘤细胞的增殖，侵袭和转移有密切关系。     

As2O3对ACC-2细胞增殖及其表达VEGF和bFGF的影响

目的：本实验研究三氧化二砷（As2O3）对人类腺样囊性癌ACC-2细胞体外增殖及其表达VEGF和bFGF的影响。探讨As2O3对人类腺样囊性癌VEGF和bFGF基因的作用机制。方法：采用MTT法检测不同浓度和作用时间下As2O3对ACC-2的增殖／抑制效应，倒置显微镜下观察腺样囊性癌ACC-2细胞生长情况；以RT-PCR、Western blotting方法检测As2O3作用后腺样囊性癌ACC-2细胞的血管生成相关因子VEGF和bFGF表达的变化。结果：As2O3作用48h内对ACC-2细胞有促进生长作用，48、72h对细胞有明显抑制，并呈时间．剂量依赖关系。RT-PCR检测显示．As2O3作用后腺样囊性癌ACC-2细胞VEGF和bFGF基因表达无明显变化。Western blotting检测显示，腺样囊性癌ACC-2细胞VEGF和bFGF蛋白表达与As2O3药物浓度和作用时间呈负相关。结论：（1）As2O3体外作用人类腺样囊性癌ACC-2细胞后48h内有促生长作用，48h后有明显抑制作用；（2）As2O3可明显抑制腺样囊性癌ACC-2细胞血管生成相关因子VEGF和bFGF基因蛋白表达，可能具有抗腺样囊性癌血管形成的作用。     

NF-κB(p65)蛋白核转位与唾液腺腺样囊性癌侵袭和转移关系的研究

目的检测NF-κB p65蛋白在唾液腺腺样囊性癌（adenoid cystic carcinoma,ACC）临床组织标本中的表达水平和转位情况,分析其与ACC侵袭和转移之间的关系。方法应用免疫组织化学法,检测58例唾液腺腺样囊性癌石蜡标本中p65蛋白的表达和细胞定位,分析p65蛋白的表达、转位与ACC转移和其他临床病理参数的关系。结果在转移和非转移的ACC组,p65细胞表达阳性率无显著差异（P=0.2641）;而p65蛋白的核阳性表达率ACC转移组明显高于非转移组（P=0.0261）,并且与ACC神经浸润相关（P=0.0244）。结论 NF-κB通路的激活可能在唾液腺腺样囊性癌侵袭和转移中发挥重要作用。     

涎腺腺样囊性癌细胞株p16基因缺失、突变及表达意义

目的　研究 p16基因在涎腺腺样囊性癌细胞株中的缺失和突变等结构变化及其表达之间的关系。方法　应用聚合酶链反应 (PCR)和 PCR-单链构象多态性 (SSCP)对涎腺腺样囊性癌细胞株 ACC- 2和高转移细胞克隆 ACC- M进行 p16基因缺失、突变的检测 ,应用免疫组化方法检测 p16基因在细胞株中的蛋白表达。结果　涎腺腺样囊性癌细胞株 ACC- 2检测 p16基因阳性 ,高转移细胞克隆 ACC- M缺失 ,两个细胞克隆均无点突变 ,ACC- 2的p16蛋白表达阳性 ,而 ACC- M蛋白表达阴性。结论　 p16基因在高转移涎腺腺样囊性癌克隆中的缺失 ,表明 p16基因在涎腺腺样囊性癌的演进和转移中具有抑癌作用。     

人涎腺腺样囊性癌中表皮生长因子受体的表达及意义

目的:研究表皮生长因子受体(EGFR)在人涎腺腺样囊性癌组织和细胞系中的表达及其临床病理学意义。方法:采用免疫组化S-P法对86例不同亚型的人涎腺腺样囊性癌组织病理切片、20例正常腮腺组织切片及ACC-2细胞爬片进行EGFR的检测;激光共聚焦显微镜对ACC-2细胞中EGFR的表达情况进行检测。结果:在涎腺腺样囊性癌组织中EGFR呈过度表达,其阳性率要明显高于正常腮腺组织(P<0.05);EGFR的表达在腺样囊性癌的恶性程度相关(P<0.05);ACC-2细胞中也检测到了EGFR的过度表达。结论:EGFR表达与腺样囊性癌恶性度及预后密切相关。     

转化生长因子β1/细胞外信号调节激酶/基质金属蛋白酶通路对腺样囊性癌侵袭和迁移的影响

目的 探讨转化生长因子β1/细胞外信号调节激酶/基质金属蛋白酶2(TGF-β1/ERKl/2/MMP-2)通路在腺样囊性癌(ACC)侵袭和迁移过程中的作用及机制.方法 以腺样囊性癌ACC-2细胞株为研究对象.用转化生长因子β1(TGF-β1)以及ERK通路抑制剂UO126处理ACC-2细胞.MTT检测ACC-2细胞的增殖情况,Transwell实验检测细胞迁移、侵袭能力,Westem blot蛋白印迹检测ACC-2细胞中ERKI/2的活化及MMP-2的表达情况,实时荧光定量聚合酶链反应检测ACC-2细胞中MMP-2的mRNA的表达情况.结果 TGF-β1及UO126干预后,ACC-2细胞增殖能力无明显变化;TGF-β1刺激可增强ACC-2细胞迁移、侵袭能力,增加ACC-2细胞p-ERKI/2和MMP-2蛋白以及MMP-2 mRNA的表达.而UO126阻断ERK磷酸化后,抑制了TGF-β1刺激的增强作用,ACC-2细胞的迁移、侵袭能力降低,MMP-2蛋白和mRNA的表达均下降.结论 TGF-β1/ERKl/2/MMP-2通路参与了人唾液腺ACC侵袭和迁移能力的调节.TGF-β1可通过上调ERK1/2,继而上调MMP-2,促进人唾液腺ACC细胞的侵袭和迁移能力,ERKl/2可能成为人唾液腺ACC侵袭防治的新靶点.     

Evidence of the validity of a model of determinants of quality of restorative dental care

A model describing the relationship between self-reported quality of restorative dentistry and dentist characteristics for 119 Montana general dentists is presented. The best predictors formed a significant model explaining 22% of the variance of the quality measure. Results are contrasted with a previous estimation of the model for 102 Washington general practitioners. Evidence for the external validity of the model is presented.     

Experimental evidence of formation of imines in the course of reduction of hydrazones

The reduction of hydrazones is generally suggested to proceed through a reductive cleavage of the nitrogen–nitrogen bond followed by a reduction of the carbon–nitrogen bond. This sequence of reduction processes is here supported for fluorenone (V) and benzophenone (VI) hydrazones as well as by a comparison of the reduction of fluorenone and benzophenone hydrazonium ions (I,III) with corresponding imines (II,IV). Another proof of the presence of imines as intermediates is the splitting of four-electron waves of hydrazones V and VI and hydrazonium ions I and VIII into two waves at pH < 2. This has been interpreted as due to differences in slopes dE_1/2/dpH and pK_a-values of protonated hydrazine derivatives on one side and corresponding imines on the other. In this pH-range imines formed in reductions of VI and VIII are reduced in a single two-electron wave, those of I and V in two one-electron steps. Fluorenone imine (II) is sufficiently stable to allow recording of time-independent current–voltage curves between pH 6 and 11. In this pH-range the imine (II) is reduced in two one-electron steps. Benzophenone imine (IV) has been found stable between pH 4.6 and 12. At pH 4.6–8 the reduction of the imine IV takes place in a single two-electron step, at pH 8–12 in two one-electron steps. Final proof of the initial cleavage of the N–N bond is presented by comparison with the reduction of nitrones.     

不同剂型玻璃离子水门汀溶解性比较研究

目的:研究、比较不同剂型玻璃离子水门汀的溶解性和表面微观形态改变,为临床使用提供依据.方法:将3M树脂加强型玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(水粉剂型)及GC玻璃离子水门汀(双糊剂型)分别在人工唾液中浸泡30 d,冷热循环15000次,烘干测重,比较前后质量变化,计算溶解率,并用扫描电镜观察表面微观改变.结果:不同剂型的玻璃离子水门汀溶解率由高到低分别为3M树脂加强型玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(双糊剂型).3种玻璃离子水门汀经浸泡溶解后,SEM扫描表面微观形态可观察到GE玻璃离子水门汀(双糊剂型)表面形态改变较少,其他2组玻璃离子水门汀表面微观改变较多.结论:双糊剂型玻璃离子水门汀理化性能及溶解率均低于传统水粉剂型,是未来临床修复治疗的的良好选择.     

Design of clinical trials of traditional therapies of periodontitis

The present paper on the design of clinical trials of periodontal therapy first addresses the issue of the etiology of periodontal disease. It is suggested that most if not all forms of destructive periodontal disease are caused by microorganisms and that there are different forms of disease with different microbial etiologies. The progressive nature of destructive periodontal disease is subsequently discussed and it is emphasized that, in a given patient, periodontal sites which show signs of inflammation and attachment loss may not over a period of several months and years show further sign of attachment loss. The present methods of assessing periodontal disease do not allow us to discriminate between potentially active and inactive sites in untreated patients. The significance and variability of indicators of periodontal disease such as bleeding on probing, probing pocket depth and probing attachment level measurements are discussed. The errors inherent in the various measurements are analyzed and suggestions are presented describing how alterations in any of the above parameters could be identified and presented in a clinical trial. Of concern for the statistical analysis of clinical data of periodontal disease is the definition of the "experimental unit". For a number of years, the "experimental unit" in periodontal trials was the patient. It is clear, however, that different sites within the same individual show different patterns of disease progression and lesion morphology and often respond differently to periodontal therapy. Statistical analyses must consequently be designed which recognize differences in site-to-site infection and lesion morphology within a common host. Until such analyses are available, the investigator should be wary of pooling data within the same individual, since such pooling may obscure meaningful alternatives which may take place in individual periodontal sites. Some goals of periodontal therapy are subsequently identified. 4 goals are discussed more in detail, namely: to establish conditions which will allow the patient to maintain a dentition without further breakdown of the periodontium; to reduce pocket depth to establish an anatomy in the dentogingival region which with proper maintainance care will prevent the re-establishment of the subgingival infection; to gain attachment as a result of treatment; to assess the effect of a certain chemotherapeutic agent on periodontal disease.     

Evaluation of efficacy of chemiluminescence for diagnosis of leukoplakia

ObjectiveLeukoplakia is the most common potentially malignant disorder preceding oral cancer. Chemiluminescence has been developed as an adjunct to conventional examination for the diagnosis of these potentially malignant disorders. This study was conducted to assess the efficacy of chemiluminescence in the diagnosis of leukoplakia and to compare the results with histopathological examination.Study designA total of 50 patients with leukoplakia were included from the outpatients attending the Department of Oral Medicine and Radiology, Dental Hospital, Bengaluru, Karnataka, India. These patients were subjected to conventional oral examination followed by chemiluminescent examination with Vizilite (Zila, Fort Collins, CO, USA) and biopsy for histopathological confirmation.ResultsThe sensitivity, specificity, positive predictive value, and negative predictive value of chemiluminescence were 93.75%, 55.56%, 78.95%, and 83.3%, respectively. The overall accuracy of chemiluminescence was 80%. A statistically significant association was observed between histopathology results and chemiluminescence results.ConclusionAlthough it is an easy, safe, minimal time consuming, and noninvasive technique, it has only adjunctive utility and it does not replace biopsy for the diagnosis of leukoplakia.     

颌骨动静脉畸形的栓塞治疗

目的：总结直接穿刺结合经血管内介入栓塞治疗颌骨动静脉静脉畸形的经验。方法：收治凳骨动静脉畸形患者6例,均进行了介入栓塞治疗。采用的栓塞材料为附凝血棉纤毛的螺圈,聚乙烯醇泡沫微粒和二氰基丙烯酸对丁酯。数字减影颈动脉造影在PHILIPSV300下完成。结果6例颌骨动静脉畸形患者中4,例急性出血得到了快速、有效控制,1例慢性渗血的右下 骨动静脉畸形患者,介入栓塞治疗,拔除松动的右下凳第一磨牙,有效地控制了出血,另1例伴局部软组织搏动性膨隆的上凳骨动静脉畸形患者,介入治疗后膨隆的搏动性得到明显改善,栓塞治疗后分别随访3－24个月,均未发现有口腔内渗血或出血。随访的X线片上,病灶区可见新骨形成。结论：局部穿刺结合经血管内介入栓塞治疗颌骨动静畸形是一种安全、有效的治疗方法。     

正畸患者切牙影像失真发生情况分析

目的研究正畸患者曲面体层片上的切牙影像失真发生情况，并分析其原因。 方法从中山大学附属口腔医院放射科影像数据库中选取500例正畸患者的曲面体层片和头影测量侧位片，所有曲面体层片均采用咬合杆投照，分别从切牙牙体影像放大、缩小、牙根变短、根尖模糊等评价指标分析上下颌切牙影像失真的发生情况，在头影测量侧位片上测量中切牙根尖-对颌切牙切缘的距离，探讨切牙影像失真发生的原因。采用SPSS 19.0统计软件对所得数据进行统计学检验。 结果500例患者中，切牙牙体影像正常者共417例，切牙牙体影像失真者共83例，影像失真发生率16.6%，其中切牙牙体影像放大17例、牙体影像缩小0例、牙根变短30例，牙根影像变短伴模糊36例。影像失真患者的根尖-切缘距离大于影像正常的患者，差异有统计学意义（F = 5 187.18，P = 0）；影像失真患者的覆盖值大于影像正常的患者，差异有统计学意义（F>477，P = 0）。 结论严重牙颌面畸形如反 、深覆盖是导致曲面体层片的切牙影像失真的主要原因之一。     

正常青年Monson球面半径的测量研究

目的测量正常青年Monson球面半径。方法选择60名(男30名,女30名)正常青年制取全口印模,应用立体摄影成像的原理与方法对Monson球面半径进行测量和统计学处理。结果Monson球面的半径平均为10.173 cm,大于理论值10.160 cm,差异有显著性(P<0.01);男、女性球面半径差异无显著性。结论本实验所得到的数据可作为全口义齿修复中记录颌位关系的一个参量。     

鼻测量法的进展

唇裂术后继发畸形是指唇裂修复术后,仍遗留或继发于手术操作和生长发育变化而表现出来的一类畸形[1]。包括唇畸形、鼻畸形和颌骨畸形。其修复较原发性唇裂修复更复杂,更灵活多变。而导致其修复复杂性的一个重要原因即是局部组织结构复杂变异和缺乏可靠的三维测量手段[2],鼻畸形