共查询到18条相似文献,搜索用时 187 毫秒
1.
2.
东北地区少数民族基因组保存永生细胞库的建立 总被引:1,自引:0,他引:1
作者采用EBV转化,同时加入环孢霉素A方法,对世居东北地区的鄂温克、鄂伦春和达斡尔3个少数民族建立了淋巴母细胞永生细胞库,共建系91个,人类基因组多样性研究,是探讨各民族的起源,演进,研究基因突变与遗传病发生的重要组成部分。建立永生细胞库保存少数民族个体基因组,可为今后遗传多样性的研究提供长期的研究资料。 相似文献
3.
4.
中国人精神分裂症患者永生细胞库的建立 总被引:3,自引:0,他引:3
目的 保存精神分裂症特有的基因组资源,方法 采用Epstein Barr(EB)病毒转化技术,把患者外周血B淋巴细胞转化成永生类淋巴母细胞系。结果 建系150个,包括精神分裂症各型、同胞对、双胞胎、核心家庭、三代及三代以旧家系构成永生细胞库。结论 精神分裂症患者基因组保存永生细胞库的建立,为我国精神分裂症分子水平发病机理的研究提供用之不竭的DNA资源。 相似文献
5.
6.
7.
灯光诱捕法吸血蠓类调查 总被引:3,自引:1,他引:2
本文报告了1992年6~9月在闽北山区建(区卂)市,采用畜、禽舍灯光诱捕法,观察了蠓类对不同宿主的趋吸习性,诱获吸血蠓2属23种,趋吸牛、猪、鸡和鸭各19、20和7种,牛舍诱获虫数占89.4%。肩库蠓和抚须库蠓为当地优势蠓种,分别占35.3%和32.7%。所有诱获蠓平均吸血率为72.7%,刺螯库蠓高达93.5%。6月中旬为当地吸血蠓活动高峰期。 相似文献
8.
9.
10.
11.
本文采用ELISA法检测247名职业健康献血浆员中抗-HCV和HBV感染标志(HB-VM),并用PCR技术检测其中85人血清HBV-DNA的存在状况.发现抗-HCV阳性率为4.0%,HBVM阳性率为29.1%,HBV-DNA阳性率为11.8%≥40岁人群抗-HCV阳性率明显高于30岁以下人群(7.7%比0,P=0.042).HBVM阳性者抗-HCV阳性率明显高于HBVM阴性者(8.3%比2.3%,P=0.038),HBV感染与HCV感染之间存在一定的伴随关系.HBVM阳性者血清HBV-DNA阳性率明显高于HBVM阴性者(18.2%比 0,P<0.05).结论认为目前常规筛选献血员的方法不安全,建议加以改进. 相似文献
12.
13.
Meng Xian-yong孟宪镛 Xu Ke-cheng徐克成and Yang Zhen-hua杨振华Nantong Medical College Nantong 《中华医学杂志(英文版)》1987,100(2):146-148
Serum enzyme aralysis has been used in the
diagnosis of hepatobiliary diseases for many
years. In order to enhance the diagnostic value
of enzymology, attention has been directed to
the different molecular forms (isozymes) of a
given enzyme. Since 1977, we have studied the
clinical application of isozymes of lactate dehy-
drogenase (LDH), glutamate-oxalacetate transa-
minase (GOT), r-glutamyl transpeptidase
(GGT), alkaline phosphatase and adenosine dia-
minase. The results of our study of LDH is-
ozyme and GOT isozyme are reported in this
paper. All cases were hospitalized and evaluated
carefully. The diagnosis was made according to
the widely accepted clinical criteria and in about
half of the cases the diagnoses were proven by
pathological examination through_biopsy and/or
operation. Normal sera were obtained from
blood bank donors in our hospital. 相似文献
14.
The National Blood Policy in India relies heavily on voluntary blood donors, as they are usually assumed to be associated with low levels of transfusion-transmitted infections (TTIs). In India, it is mandatory to test every unit of blood collected for hepatitis B, hepatitis C, HIV/AIDS, syphilis and malaria. Donors come to the blood bank with altruistic intentions. If donors test positive to any of the five infections, their blood is discarded. Although the blood policy advocates disclosure of TTI status, donors are not, in practice, informed about their results. The onus is on the donor to contact the blood bank. Out of approximately 16 000 donations in the past 2 years, 438 tested positive for TTI, including 107 for HIV. Only 20% of the donors contacted the blood bank; none of them were HIV positive. Disclosure by blood banks of TTI status by telephone or mail has resulted in serious consequences for some donors. Health providers face an ethical dilemma, in the absence of proper mechanisms in place for disclosure of test results, regarding notification to donors who may test positive but remain ignorant of their TTI status. Given the high cost of neglecting to notify infected donors, the authors strongly recommend the use of rapid tests before collecting blood, instead of the current practice, which takes 3 h to obtain results, and disclosure of results directly to the donor by a counsellor, to avoid dropouts and to ensure confidentiality. 相似文献
15.
Antisexum against purified cytoplasmic granules from rat LGL tumor cells, and protein A-gold inmmnoelec-tron microscopy were used to study the secretory events in lysis of YAC-1 tumor cells by rat LGL tumor cells or by isolated LGL from normal rats. After 30 min incubation of effector and target cells together, gold-labeled cyto-plasmic granules were often seen concentrated in the area of the LGL adjacent to the ~ YAC-1 Within 60min,the grantees were observed to move to the cell border near the conjugazed site. At this point, fine granules were fused with file cell membrane, and subsequently released file gold-labeled contents into the junction between the LGL and the target cell. Gold particles could be seen at the B-T interface, on the surface,or sometimes on the target cell surface.These data provide direct evidence for the hypothesis that under conditions of active cytotoxicity,natural killer cells secrete their cytoplasmic granule contents leading to the deposition of granule material on the target cell surface and the eventual lysis of the cell. 相似文献
16.
目的分析仙桃市无偿献血血液标本检测情况,探讨血液标本不合格因素及输血相关病原体感染的危险性,为临床安全用血提供技术支持。方法对仙桃市血站2006年1月~2010年12月31602名献血人员资料进行分析,比较不同性别、不同年龄献血者血液检测不合格率。结果无偿献血者血液总标本数31602份,检测合格标本数30330份,检测合格率为95.98%;检测不合格标本数1272份,检测不合格率为4.02%。其中ALT阳性检测不合格率2.13%;HBsAg阳性检测不合格率1.02%;抗-HCV阳性检测不合格率0.35%;SYP阳性检测不合格率0.38%;抗-HIV阳性检测不合格率0.15%。结论了解和掌握无偿献血群体、制定符合临床用血规律的采供血计划十分必要,而实行血液集中检测、严格筛查经血液感染性疾病直接关系到供血者的身体健康和受血者的安全,是提高临床安全用血的关键。 相似文献
17.
The National Blood Policy of India, 2002, advocates the disclosure of results of transfusion transmitted infections (TTI) to blood donors. However, in the absence of well-defined notification processes, and in order to avoid serious consequences resulting from unguided disclosure, blood bank personnel discard blood that is TTI-positive. We report on a survey of 105 voluntary blood donors in Kerala. Only two out of three participants had filled the donor form in the last year. Only half were aware that the blood bank was supposed to inform them if they tested positive for TTI. Fifty-seven per cent of donors wanted to be informed every time they donated blood, irrespective of a positive or negative result. 相似文献
18.
目的探讨国内血站对献血员乙型肝炎病毒表面抗原(HBsAg)筛查的安全性和可靠性。方法收集经血站筛查HBsAg阴性的合格献血员血浆983份,用雅培Architect12000电化学发光仪定量检测HBsAg;巢式PCR扩增乙肝病毒S区、C区、X区。诊断为隐匿性乙肝病毒感染者,巢式PCR扩增病毒PreS/S区,产物直接测序并与标准病毒序列对比分析病毒株变异。结果经雅培Architect I2000复检HBsAg弱阳性率为6.5%(64/983),HBsAg滴度中位数为0.171U/ml;60.9%(39/64)病毒两个区扩增阳性,4.0%(39/983)健康献血员为隐匿性HBV携带者;35.9%(14/39)病毒PreS/S区段扩增阳性,未发现“a”决定簇内的变异株。结论目前经血站常规检测ItBsAg为阴性的合格血液用敏感性更高的试剂仍能检测出HBsAg及HBV DNA。 相似文献