KLF-6在口腔扁平苔藓恶变中的表达研究

目的 探讨KLF 6在口腔扁平苔藓发病机制及癌变中的作用。方法 分别采用免疫组化和Western blot方法对10例正常口腔黏膜、30例扁平苔藓患者和22例口腔鳞癌患者的上皮组织中KLF 6蛋白进行t检测,比较其在三者之间的差异。SPSS 14.0对数据进行检验。结果 扁平苔藓患者组织中KLF 6蛋白的表达明显低于正常口腔黏膜,两组之间具有显著性差异;口腔鳞癌患者组织中KLF 6蛋白的表达明显低于扁平苔藓组织,两组之间具有显著性差异。结论 KLF 6的表达异常可能在口腔扁平苔藓的发生发展及癌变的过程中起作用。     

口腔黏膜病

银杏叶提取物对大鼠复发性口疮模型外周血T淋巴细胞的影响；金因肽治疗糜烂型扁平苔藓疗效观察；干燥综合征患者唾液pH值、电解质测定的诊断意义初步探讨；口腔扁平苔藓、白斑及鳞癌棘细胞形态计量学分析；口腔黏膜下纤维性变中u—PA和PAI-1的表达及意义；口腔干燥症患者唾液分泌与相关因素分析；萎缩性舌炎病因探讨及治疗。[编者按]     

口腔扁平苔藓黏膜成纤维细胞KGF的表达水平检测

目的 检测体外培养的正常口腔黏膜与口腔扁平苔藓黏膜成纤维细胞表达和分泌的角质细胞生长因子(KGF)的量。方法 应用酶联免疫吸附剂测定(enzyme-linked immunosorbnent assay,ELISA)技术检测体外培养的14例口腔扁平苔藓黏膜成纤维细胞和11例正常口腔黏膜成纤维细胞分泌的角质细胞生长因子蛋白的情况。提取各组细胞RNA,用逆转录-聚合酶链反应(RT-PCR)法检测KGF mRNA表达量的变化。结果 ELISA测定结果显示,扁平苔藓黏膜成纤维细胞分泌角质细胞生长因子蛋白量低于正常口腔黏膜,经检验差异有统计学意义(P0.05)。结论 与正常口腔黏膜相比,口腔扁平苔藓黏膜成纤维细胞分泌的角质细胞生长因子蛋白水平降低,而在基因水平上没有变化。     

应用双向电泳和质谱技术筛选口腔扁平苔藓差异蛋白

目的:筛选口腔扁平苔藓及正常黏膜组织的差异表达蛋白,为口腔扁平苔藓的发病机制及诊断治疗的研究提供实验室依据.方法:收集口腔扁平苔藓组织及正常口腔黏膜组织,提取组织蛋白,蛋白定量,进行双向电泳,图象分析寻找差异点,基质辅助激光解吸飞行时间质谱(MALDI-TOF-MS)分析鉴定表达差异量较大的蛋白质点,确定所分析的蛋白质类型和功能.结果:(1)人口腔扁平苔藓及正常黏膜组织的双向电泳图谱的平均蛋白质点数分别为1 576±67和1 608±73,同一组织凝胶在蛋白质点位置上重复性较好.(2)通过比较人口腔扁平苔藓与正常黏膜组织的双向凝胶电泳图谱,得到差异表达蛋白质点数为13个,其中7个点在口腔扁平苔藓中为高表达,6个点在口腔扁平苔藓中为低表达.选择1O个表达差异量较大的蛋白质点进行质谱和生物信息学分析,鉴定了其中的4个点,包括有锰超氧化物歧化酶(Mn-SOD),膜联蛋白I,波形蛋白,未知蛋白等.结论:锰超氧化物歧化酶,膜联蛋白I,波形蛋白,未知蛋白可能参与了口腔扁平苔藓的发生和发展,其相关机制尚待进一步阐明.双向电泳-质谱分析技术为口腔扁平苔藓发生发展过程中差异表达蛋白的研究提供了有效的技术手段.     

口腔扁平苔藓患者血浆纤维结合蛋白和纤维蛋白原的测定和分析

采用速率散射比浊法，对１４例口腔扁平苔藓患者的血浆纤维蛋白原（Ｆｇ）、纤维结合蛋白（Ｆｎ）进行检测，结果发现患者的Ｆｎ含量高于健康对照组，Ｆｇ含量无明显变化。作者认为Ｆｎ的增高与口腔扁平苔藓患者血小板对诱聚剂反应增强，Ⅱ相聚集曲线高陡有直接的关系。     

CCR6-CCL20轴在口腔扁平苔藓黏膜组织中的表达及意义

目的检测口腔扁平苔藓(oral lichen planus,OLP)黏膜及正常口腔黏膜中趋化因子CCL20(CC-chemokine ligand 20,CCL20)及其受体CCR6(CC-chemokine receptor 6,CCR6)的表达,研究CCR6-CCL20轴在口腔扁平苔藓病中的作用。方法通过免疫组织化学及荧光定量PCR分别检测口腔扁平苔藓病损组织及正常口腔组织中CCR6和CCL20的蛋白及mRNA的表达。结果 CCR6及CCL20在口腔扁平苔藓患者的黏膜组织中的表达均明显高于正常人,且具有统计学差异(P<0.000 1)。结论 CCR6-CCL20轴参与了口腔扁平苔藓的发生和发展,促进黏膜病损的产生及炎症细胞的浸润。     

口腔黏膜病

20062359口腔扁平苔藓患者白色念球菌分离株的颊细胞粘附力研究；20062360p16和p53蛋自在口腔白斑和鳞状细胞癌中表达的比较研究；20062361兔口腔黏膜固有层多能干细胞的分离培养；20062362药物雾化治疗疱疹性口炎的临床研究；20062363口腔黏膜下纤维性变组织中Smad2／3蟹白的表达研究；20062364226例唇部疾病的前瞻性分析；20062365口腔扁平苔藓组织中表皮生长因子及其受体表达变化的研究；20062366口腔黏膜色素异常与恶性病变（综述）；20062367人类自细胞抗原与口腔扁平苔藓的关联及其机制（综述）；20062368抗疱疹病毒药物的研究进展（综述）；20062369浅议标准化在口腔黏膜病学中的应用.[编者按]     

口腔癌前病变细胞凋亡状况及Bcl-2、Bax表达的研究

目的 :通过对口腔白斑、扁平苔藓病变发展及癌变过程中细胞凋亡状况和凋亡相关蛋白表达水平的研究 ,探讨口腔白斑、扁平苔藓的癌变机理及病变机制。方法 :采用原位末端转移酶标记法及免疫组化法 ,观察分析 10例正常口腔黏膜上皮 ,18例扁平苔藓 ,2 3例白斑 ,2 2例口腔鳞癌上皮组织中细胞凋亡状况及凋亡相关蛋白Bcl- 2、Bax的表达水平。结果 :上皮 (轻 ,中 ,重 )不典型增生 ,鳞癌及糜烂型扁平苔藓的凋亡指数均高于正常 (P <0 .0 1)。Bcl- 2在白斑、扁平苔藓上皮细胞层无异常表达 ,但在扁平苔藓固有层淋巴细胞浸润处过度表达 ,在鳞癌组织中显高表达 (P <0 .0 1)。Bax在上皮单纯增生、轻、中度不典型增生和鳞癌及糜烂型扁平苔藓组织中显过度表达 (P <0 .0 5 )。结论 :白斑、扁平苔藓的病变发展及癌变过程中 ,上皮细胞凋亡状况发生了改变 ,Bax参与了白斑癌变的早期事件。扁平苔藓的发病机制与Bcl- 2、Bax在特定部位的过度表达有关     

复方苦参含漱液治疗口腔扁平苔藓的临床及细菌学研究

目的：观察复方苦参含漱液治疗糜烂型扁平苔藓的临床疗效,以及治疗前、后患者口腔内细菌的检出量、检出率及构成比的变化。方法：采用复方苦参含漱液治疗糜烂型扁平苔藓患者30例,采用SAS6.12软件包中的配对t检验比较用药前、后患者疼痛指数的变化,实验室检查治疗前、后患者口腔唾液细菌检出量、检出率和构成比的变化。对细菌检出率、构成比进行χ^2检验,处于非正态分布的细菌检出量,取其常用对数值进行非参数相关检验。结果：用药1周后,患者疼痛指数显著下降（P〈0.01）。糜烂型扁平苔藓患者口腔内细菌总检出率、检出量减少。葡萄球菌的检出量显著减少（P〈0.05）;其他唾液细菌的检出率和检出量虽有减少,但无统计学意义。细菌构成比无显著性差异。结论：复方苦参含漱液对口腔糜烂型扁平苔藓患者唾液葡萄球菌有抑制作用,是一种局部治疗口腔扁平苔藓的有效药物,可作为全身用药的辅助治疗。     

口腔扁平苔藓基因表达谱中差异表达基因初步探讨

目的筛选口腔扁平苔藓组织中的差异表达基因,并对其进行功能分类。方法用4 000种人类基因多聚酶链反应产物制成BiostarH- 40s型表达谱芯片,分离纯化正常口腔黏膜组织和口腔扁平苔藓病变组织mRNA,制备表达谱探针,用ScanArray 4000 荧光扫描仪扫描芯片荧光信号图像,分析正常口腔黏膜组织和口腔扁平苔藓组织之间差异表达的基因。结果1）在4 000条基因中,有213条基因表达差异,其中122条基因表达上调,91条基因表达下调。2）在表达上调基因中,功能分类主要包括免疫相关基因、代谢相关基因、癌基因、细胞因子、细胞信号和传递蛋白。3）在表达下调基因中,功能分类主要包括DNA结合、转录和转录因子、细胞信号和传递蛋白、免疫相关基因、细胞因子、代谢相关基因。结论口腔扁平苔藓的发生、发展过程中存在着多条不同功能基因表达调控的改变。     

Book Reviews

Book reviewed in this article:
HANDBOOK OF LOCAL ANAESTHESIA, by S. F. Malamed.
ENDODONTIC PRACTICE, by Louis I. Grossman.
PRINCIPLES OF PATHOLOGY FOR DENTAL STUDENTS, by J. B. Walter, Margaret C. Hamilton, and M. S. Israel.
COMPLETE HANDBOOK FOR DENTAL AUXILIARIES, by C. A. Reap, Jr.
TOOTH SURFACE INTERACTIONS AND PREVENTIVE DENTISTRY, edited by G. Rolla, T. Sonju, and G. Embery.
CLINICAL ORAL PEDIATRICS, edited by G. E. White.
FUNDAMENTALS OF FIXED PROSTHODONTICS, by H. T. Shillingburg Jr, S. Hobo, and L. D. Whitsett.
RESTORATIVE TECHNIQUES FOR INDIVIDUAL TEETH, Masson Monographs in Dentistry 2, edited by L. Baum.
OCCLUSION IN CLINICAL PRACTICE, Dental Practitioner Handbook No. 30, by H. Thomson.
EPILEPSY AND THE ORAL MANIFESTATIONS OF PHENYTOIN THERAPY, by T. M. Hassell
LOCAL ANAESTHESIA IN DENTISTRY, by G. L. Howe, and F. I. H. Whitehead.
METALS FORUM, Volume 3, Number 4, 1980, assembled by J. J. Stobo.
McCRACKEN'S REMOVABLE PARTIAL PROSTHODONTICS, by D. Henderson, and V. L. Steffel.
DENTAL PUBLIC HEALTH AND COMMUNITY DENTISTRY, edited by A. Jong.
TEXTBOOK OF OPERATIVE DENTISTRY, by L. Baum, R. W. Phillips, and M. R. Lund.
A MANUAL OF PAEDODONTICS, by R. J. Andlaw and W. P. Rock.
ORAL SURGERY IN DENTAL PRACTICE, by E. Kruger, and P. Worthington.
NUTRITION IN PREVENTIVE DENTISTRY: SCIENCE AND PRACTICE, edited by A. E. Nizel.
REGIONAL ANAESTHESIA OF THE ORAL CAVITY, by J. T. Jastak, and J. A. Yageila.
DENTAL RADIOLOGY, by A. H. Wuehrmann, and L. R. Manson-Hing.     

Book Reviews

Book reviewed in this article:
TOOTHACHE AND OROFACIAL PAIN, by J. M. Mum-ford.
INTRODUCING WHO. Geneva, World Health Organization, 1976.
PROSTHODONTIC TREATMENT FOR EDENTULOUS PATIENTS, by C. O. Boucher. J. C. Hickey, and G. A. Zarb.
A COLOUR ATLAS OF HISTOLOGY, by M. B. L. Craigmyle.
ORTHODONTIC DIAGNOSIS. Dental Practitioner Handbook No. 4, by W. J. B. Houston.
WALTHER'S ORTHODONTIC NOTES, revised by W. J. B. Houston.
DENTAL IDENTIFICATION AND FORENSIC ODONTOLOGY, by W. Harvey and 15 contributors.
COMMUNICATION IN THE DENTAL OFFICE. A PROGRAMMED MANUAL FOR THE DENTAL PROFESSIONAL, by R. E. Froelich, F. M. Bishop, and S. F. Dworkin.
MASTICATION. PROCEEDINGS OF A SYMPOSIUM ON THE CLINICAL ASPECTS OF MASTICATION. MEDICAL SCHOOL, BRISTOL, 14–16 APRIL, 1975, edited by D. J. Anderson and B. Matthews.
ESSENTIALS OF CLINICAL DENTAL ASSISTING, by J. E. Chasteen.
THE USE OF ADHESIVES IN DENTISTRY, by M. G. Buonocore.
ENDODONTIC THERAPY, edited by Franklin S. Weine, with five contributing authors.     

Book Reviews

Book reviewed in this article:
CLINICAL PHARMACOLOGY IN DENTAL PRACTICE, edited by Sam V. Holroyd.
ORAL DIAGNOSIS, by D. A. Kerr, M. M. Ash, and H. D. Millard.
THE YEAR BOOK OF DENTISTRY 1978, edited by M. L. Hale, S. P. Hazcn, R. E. Moyers, D. F. Redig, M. B. O. Robinson, S. I. Silverman.
RADIOLOGY FOR DENTAL AUXILIARIES, by Herbert H. Frommer.
WHO STUDY OF DENTAL CARIES ETIOLOGY IN PAPUA NEW GUINEA, WHO Offset Publication No. 40, by R. G. Schainschula, B. L. Adkins, D. E. Barmes, G. Charlton, and B. G. Davey.
DEVELOPMENT, FUNCTION AND EVOLUTION OF TEETH, edited by P. M. Butler, and K. A. Joysey.
FLUORIDES AND DENTAL FLUOROSIS, by Howard M. Myers.
SELF ASSESSMENT MANUAL. PART I. ORAL SURGERY AND PATHOLOGY, by B. Cohen, D. Mason, and D. Poswillo.
DICTIONARY OF ABBREVIATIONS IN MEDICINE AND THE HEALTH SCIENCES, by Harold K. Hughes.
MANUAL OF CLINICAL PERIODONTICS, edited by H. L. Ward, and M. Simring. St. Louis
CONSCIOUS SEDATION IN DENTAL PRACTICE, by C. Richard Bennett. St Louis
BEHAVIOURAL SCIENCE AND DENTAL PRACTICE, by Samuel F. Dworkin, Thomas P. Ference, and Donald B. Giddon.     

Book Reviews

Book reviewed in this article:
DENTAL LABORATORY PROCEDURES. COMPLETE DENTURES, by Robert M. Morrow, Kenneth D. Rudd and Harold F. Eissmann.
IMMUNOLOGY OF ORAL DISEASES, by I. M. Riott and T. Lehner.
PERIODONTICS, by J. D. Manson.
ATLAS OF COMPLETE DENTURES, by G. Passamonti.
TEXTBOOK OF PEDIATRIC DENTISTRY, edited by R. L. Braham and Merle E. Morris.
SEDATION, by H. L. Hamburg.
DENTAL PLAQUE. THE ECOLOGY OF THE FLORA ON HUMAN TEETH, by H. N. Newman.
FUNDAMENTALS OF REMOVABLE PROSTHODON-TICS, by D. L. Johnson and R. J. Stratton.
PRACTICE MANAGEMENT, A BUSINESS MANUAL FOR ALL PROFESSIONS AND STAFFS, by Felicity Baverstock and D. Eve.
ORBAN'S ORAL HISTOLOGY AND EMBRYOLOGY, edited by S. N. Bhaskar.
DENTAL MANAGEMENT OF THE MEDICALLY COMPROMISED PATIENT, by J. W. Little and Donald A. Falace.
PANORAMIC DENTAL RADIOGRAPHY, by L. R. Manson-Hing.
MANAGEMENT OF THE GERIATRIC DENTAL PATIENT, by K. A. Freedman.     

Book Reviews

Book reviewed in this article:
NUTRITION AND DENTAL HEALTH A.J. Rugg-Gunn (with contributions by A.F. Hackett)
PORCELAIN AND COMPOSITE INLAYS AND ONLAYS. ESTHETIC POSTERIOR RESTORATIONS. D.A. Garber, R.E. Goldstein, eds.
REMOVABLE PARTIAL PROSTHODONTICS: A CASE-ORIENTATED MANUAL OF TREATMENT PLANNING. S.K. Lechner, A.R. MacGregor
BURKET'S ORAL MEDICINE, DIAGNOSIS AND TREATMENT. M.A. Lynch, V.J. Brightman, M.S. Greenberg, eds.
PERIODONTAL REGENERATION. CURRENT STATUS AND DIRECTIONS. A.M. Poison, ed.     

Present status of osseous grafting procedures.

Osseous grafting techniques represent one mode of therapy to manage combination pocket-osseous defects. They have their greatest applicability in the intrabony defect although encouraging results have been noted in furcation and suprabony sites. Like all treatment modalities, their usage is dictated by the therapeutic objectives for specific problems and whether their respective advantages and limitations outweigh other management techniques. They are neither a panacea nor an unproven experimental venture. When properly employed, they are a valuable component of currently accepted therapy. When compared with other treatment approaches, the following relative advantages and limitations have been noted with osseous grafts: Advantages 1. Reconstruct lost periodontium. 2. Idealistic therapeutic objective. 3. Reversal of disease process. 4. Increase tooth support. 5. Enhance esthetics. 6. Improve function. Limitations 1. Additional treatment time. 2. Autograft disadvantages. 3. Availability of graft material. 4. Additional postoperative care. 5. Unique postoperative problems. 6. Variations in repair. 7. Longer post-treatment evaluation interval. 8. Predictability. 9. Greater expense. 10. Multistep therapy common. 11. Vulnerability to recurrence.     

Book Reviews

Book reviewed in this article:
SURGERY OF THE TEMPOROMANDIBULAR JOINT D.A. Keith, ed
TEMPOROMANDIBULAR DISORDERS C. McNeill, ed
DISEASES OF THE PERIODONTIUM H.N. Newman, T.D. Rees, D.F. Kinane, eds.
COLOUR ATLAS OF HUMAN ORAL HISTOLOGY H. Kitamura, M. Oda, J. A. Hess
ETHICAL QUESTIONS IN DENTISTRY J.T. Rule, R.M. Veatch
PEDIATRIC DENTISTRY: INFANCY THROUGH ADOLESCENCE J.R. Pinkham, ed.
ELEMENTS OF DENTAL MATERIALS FOR DENTAL HYGIENISTS AND DENTAL ASSISTANTS R.W. Phillips, B.K. Moore
IMMUNOLOGY OF ORAL DISEASES T. Lehner
PAEDIATRIC OROFACIAL MEDICINE AND PATHOLOGY R.K. Hall     

Book Reviews

Book reviewed in this article:
ORAL SURGERY, by Kurt H. Thoma. St. Louis.
TEXTBOOK FOR DENTAL. NURSES, by H. Levison.
ADVANCES IN FLUORINE RESEARCH AND DENTAL CARIES PREVENTION.
A MANUAL OF DENTAL AND ORAL RADIOGRAPHY, by S. Blackman and H. G. Poyton.
DENTAL. JURISPRUDENCE, by Harvey Sarner.
PRINCIPLES AND PRACTICE OF ORAL RADIOLOGIC INTERPRETATION, by H. M. Worth.
A TEXTBOOK OF ORAL PATHOLOGY, by W. G. Shafer, M. K. Hine and B. M. Levy.
BOOK OF THE YEAR (BRITANNICA). Published by EncyclopÆdia Britannica Inc.
ELECTRON MICROSCOPY OF SKIN AND MUCOUS MEMBRANE, by A. S. Zelickson and V. Francis Hartmann.     

Book reviews

Books reviewed in this article:
Periodontitis in Man and Other Animals: A Comparative Review , R. C. Page and H. E. Schroeder
CRC Manual for the Examination of Bone. C. Anderson
Basic Research and Clinical Medicine S. P. Bralow, ed.
Oral Immuogenetics and Tissue Transplantation G. R. Riviere and W. H. Hildeman eds.
The Chemistry and Biology of Mineralized Tissues , ed. A. Veis
Histochemistry: The Wedding of its Applications in the Biomedical Sciences , eds. P. J. Stoward & J. M. Ploak
Diagnostic immunohistochemistry , ed. R. D. DeLellis
Biology and Disease of Dermal Pigmentation , ed. T.B. Fitzpatrick et al.
Human Dento-facial Growth D. H. Goose and J. Appleton     

Book reviews

Book reviewed in this article:
P reventive D entistry for the. A G uidebook of P ositive P reventive D ental C are , by J. Edward Shephard.
R emovable C losure of the I nterdental S pace (C.I.S.), by Arnold Gyrny.
M aterials S cience in D entistry , by E. H. Greener, J. K. Harcourt, and E. P. Lautenschlager.
O rthodontics : P rinciples and P ractice , By T. M. Graber.
S urface C hemistry and D ental I nteguments , edited by Lasslo, A., and Quintana, R. P. Springfield, Ill., Charles C. Thomas, 1973.
T ooth -C oloured F illing M aterials in C linical P ractice . Dental Practitioner Handbook No. 16, by L. W. Deubert and C. B. G. Jenkins.
F acial P rosthesis , by Arthur H. Bulbidian. Springfield, Illinois, Charles C. Thomas.
A natomy for S tudents of D entistry , by J. H. Scott and A. D. Dixon.