51例老年高粘血症血清微量元素的测定

对51例老年人进行血液流变学检查,结果表明,高粘血症组病人血清 Cu~(2+),Fe~(2+)含量高于正常组(P<0.05),血清 Ca~(2+)非常显著高于正常组(P<0.01),血清 Zn~(2+),Sr~(2+),Mn~(2+),Mg~(2+),Cr~(3+)含量及 Ca~(2+)/Zn~(2+)比值均无显著差异(P>0.05)。血清 Cu~(2+),Fe~(2+),Ca~(2+)含量多少,反映了血液粘度的变化,对高粘血症诊断有一定参考价值。     

针刺镇痛与血清金属离子浓度变化的关系

40例甲状腺腺瘤切除手术的病人分别施行颈浅神经丛阻滞或电针刺激。按不同的麻醉方式将病人随机分组。测定麻醉前后血清六种金属离子浓度。结果提示:1.针刺信号对内环境的影响可以引起血清 Ca~(2 )水平下降(P<0.05～0.01);2.针刺合谷内关时静注1.3mg/kg 哌替啶,对 Ca~(2 )的影响没有叠加作用;3.静注5%葡萄糖液有可能干扰 Ca~(2 )浓度测定值,但不影响镇痛效果;4.针刺后引起血清 Na~ 、Cu~(2 )、Mg~(2 )、Zn~(2 )浓度的一些有统计学意义的变化,值得进一步研讨。     

大同风化煤净化含重金属离子废水的研究

本文报导了用大同风化煤研制和开发一种在水中无腐植酸溶出、有较高去除水中重金属离子能力和一定强度的粒状炭质净化剂的工作。经试验证明,大同风化煤对不同金属离子的净化次序为: Pb~(2 )>Hg~(2 )>Fe~(2 )>Cu~(2 )～Cd~(2 )>Zn~(2 )>Ca~(2 )>Ni~(2 )>Mg~(2 )>Cr~(3 )。该净化剂价廉易得,效果良好,可用作处理含重金属离子废水,以期控制污染、回收金属和水的再用。本文也初步探讨了净化剂去除镍离子的机制,这将有助于这种新型净化剂的开发。这里讨论了除Ni~(2 )过程的主要因素:总酸性基团含量和碘吸附率。     

关于金属离子的酸碱性问题

人体内含有许多金属离子,如Na~ 、K~ 、Ca~( 2)、Mg~( 2)、Fe~( 3)Cu~( 2)…仅钠离子(Na~ )在血浆中的浓度就占312.8—333.5％(mgml~(-1))即135—145mEqL~(-1)。在食品及药物中金属离子也大量存在。     

膝关节骨关节炎患者尿液中C2C与Zn~(2+)、Cu~(2+)水平检测

目的探讨尿液中软骨代谢产物C2C水平和微量元素Zn~(2+)、Cu~(2+)浓度与膝关节骨性关节炎(KOA)严重程度的关系。方法收集89例KOA患者及20例非KOA患者清晨尿液标本20-25mL,置于-80冰箱低温保存,标本收齐后分别使用酶联免疫吸附试验(ELISA)检测C2C含量和采用电感耦合等离子体原子发射光谱(Inductivelycoupled plasma atomic emission spectrometry,ICP-AES)测定Zn~(2+)、Cu~(2+)浓度。分别收集KOA患者及非KOA患者膝关节正侧位平片,影像资料按Kellgren-Lawrence分级标准讨论分组,实验分为:对照组、I级组、II级组、III级组及IV级组。结果 KOA组尿液中C2C与Zn~(2+)、Cu~(2+)浓度均高于非KOA组(P0.000)。C2C水平:IV组III组II组I组和对照组(P=0.000)。Zn~(2+)、Cu~(2+)浓度:IV组、III组、II组、I组对照组(P=0.000)。结论 KOA组中尿液中C2C含量升高程度与其影像学分级呈高度正相关性。而KOA组中Zn~(2+)、Cu~(2+)浓度升高水平与其影像学分级无线性相关。尿液中C2C可用于评估KOA病情分级的生物指标;也可作为病情进展及预后监测的参考指标。Zn~(2+)、Cu~(2+)对于KOA患者早期诊断有重要参考价值;尿液中C2C与Zn~(2+)、Cu~(2+)联合有望成为KOA的早期诊断及判断其严重程度的重要指标。     

Ca~(2+)、Mg~(2+)对完整红细胞膜脂区流动性的影响

以1,6.-二苯基-1,3,5-已三烯(DPH)为荧光探剂,用荧光偏振法测定了Ca~(2+)、Mg~(2+)对完整红细胞膜脂区流动性的影响。Ca~(2+)能降低红细胞膜的流动性,而Mg~(2+)则无显著影响,细胞膜流动性的大小与膜上结合的Ca~(2+)量有关,而细胞所处的介质中Ca~(2+)浓度可影响膜上Ca~(2+)结合量。胰岛素对膜的流动性影响可能与膜上结合Ca~(2+)和Mg~(2+)相对量有关。     

咖啡因拮抗噪声引起机体损害作用的研究

噪声和咖啡因联合作用于大鼠后,观察大鼠血清及心肌中Ca~(++)、Mg~(++)含量的变化、心肌儿茶酚胺(CA)和血浆cAMP的变化,并用JEM-1200EX电镜观察了心肌超微结构的改变。结果表明,噪声可导致血清Mg~(++)含量升高,心肌Mg~(++)含量降低,心肌Ca~(++)明显升高。心肌CA和血浆cAMP含量上升。应用咖啡因后心肌Ca~(++)、Mg~(++)及血清Mg~(++)含量接近正常水平,心肌CA降低,血浆cAMP升高。提示咖啡因对可噪声所致的Ca~(++)、Mg~(++)含量异常有一定的拮抗作用,对CA和cAMP有调节作用,对噪声引起的大鼠心肌超微结构的损伤有明显减轻作用。     

癫痫与血液中钙镁含量关系的研究

用原子吸收分光光度法对未经抗癫痫治疗的10例原发性癫痫小儿患者血清 Ca~(2+),Mg~(2+)的含量进行测定;通过动物实验观察家兔血液 Ca~(2+),Mg~(2+)浓度变化对青霉素引起的癫痫样放电的影响。结果是癫痫患儿组血清 Ca~(2+)含量明显低于对照组(P<0.01),而两组 Mg~(2+)含量差异无显著性(P>0.05);当提高家兔血钙浓度时,能延长青霉素致痫的潜伏期,抑制癫痫发放的频率和振幅,而提高血镁浓度时无明显变化。动物实验结果证明了癫痫患儿血清 Ca~(2+)含量低的测定结果是合理的,当血液中 Ca~(2+)含量降低时,神经肌肉的兴奋性增高,由此可诱发痫样放电或癫痫发作。     

红细胞膜外翻囊泡钙摄取方法的建立

细胞膜上的ATP和Mg~(2 )依赖性的Ca~(2 ),Mg~(2 )-ATP酶(即钙泵)的基本功能是主动转运Ca~(2 ),维持胞内Ca~(2 )的相对恒定.细胞内Ca~(2 )浓度持续升高将会危及细胞的各种功能.细胞膜Ca~(2 )转运障碍与高血压发病有密切关系.红细胞膜是研究各种浆膜Ca~(2 )转运的理想模型.作者改进了文献方法.建立了以红细胞膜IOV来研究Ca~(2 )摄取的方法,为研究高血压发病过程中细胞膜Ca~(2 )的摄取异常机理,提供了一种可靠的手段.     

Mg~(2+)和Ca~(2+)对兔心脏腺苷酸环化酶的调节作用

本文用氧化铝层析分离、纯化cAMP测定腺苷酸环化酶(AC)活性的方法,观察了Mg~(2+)和Ca~(2+)对兔心肌质膜AC的影响。结果Mg~(2+)激活AC,其Ka为2.5mM。Ca~(2+)抑制AC,其Ki为30μM。Ca~(2+)和Mg~(2+)对兔心肌质膜AC的调节是兢争的。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.