不同产地丹参中丹酚酸B的含量测定

目的:测定不同产地丹参中丹酚酸B的含量。方法:色谱柱:Agela C18(4.6 mm×250 mm,5μm);柱温:25℃;流动相:甲醇-乙腈-甲酸-水(30∶10∶1∶59);检测波长:286 nm;流速:l ml/min。结果:五个产地丹参中丹酚酸B含量不同,四川中江(丹酚酸B,7.8%)>山东日照(丹酚酸B,6.8%)>陕西商洛(丹酚酸B,5.4%)>河北行唐(丹酚酸B,4.8%)>浙江(丹酚酸B,4.0%)。结论:各产地丹参药材中丹酚酸B含量有较大差异,以四川中江丹参的丹酚酸B含量最高,山东日照丹参次之。     

高效液相色谱法测定肝乐颗粒中丹酚酸B的含量

目的建立高效液相色谱测定肝乐颗粒中丹酚酸B含量的方法。方法50%甲醇超声提取,YWG-C18(4.6 mm×200 mm)液相色谱柱测定,流动相为甲醇∶5%乙酸水(31∶69),柱温为室温,检测波长为281 nm,流速:1 mL/m in。结果丹酚酸B的线性范围在80.27~401.35μg,r=0.9998,平均回收率为97.3%,RSD为0.54(n=5)。结论利用高效液相色谱测定肝乐颗粒中丹酚酸B的含量,操作简便,结果准确,可作为该产品质量控制的方法之一。     

HPLC测定丹参三七配伍水煎液中丹酚酸B的含量

目的　建立丹参三七配伍水煎液中丹酚酸B含量测定的方法。方法　大连依利特SpherisorbC18柱 ,流动相 :甲醇 -水 (用甲酸调pH =2 .5 ) ,体积比甲醇 -水 (4 1 5∶ 5 8 5 ) ,流速 :0 8mL/min ,检测波长 :2 80nm ,柱温 :2 5℃。结果　丹参∶三七配比 5∶ 3时 ,合煎或单煎对丹酚酸B溶出影响不大。结论　用此方法可以准确测定丹参三七配伍水煎液中丹酚酸B的含量     

高效液相色谱法测定冠心丹参胶囊中丹酚酸B的含量

目的 测定冠心丹参胶囊中丹酚酸B的含量.方法 HPLC法,YWG-C 18色谱柱(250 mm×4.6 mm,10μm),流动相∶(甲醇-乙腈):(甲酸-水)=36.7:63.3,其中甲醇:乙腈=3:1,甲酸:水=1:29,流速:1.0 mL/min,检测波长为286 nm.结果 丹酚酸B浓度在0.1～0.9 μg范围内线性关系良好(r=0.999 8,n=5).加样回收率为102.60%,RSD=1.19%(n=5).结论 本实验提取丹酚酸B处理方法简单,测定结果稳定可靠,可作为冠心丹参胶囊中丹酚酸B含量测定方法.     

双丹口服液中丹酚酸B和丹参素的稳定性研究

目的 测定双丹口服液中丹酚酸B和丹参素,并考察常温放置3个月后这2个成分量的变化。方法 采用HPLC法;色谱柱 Diamonsil C１８ (250 mm×4.6 mm,5 μm);流动相为甲醇-乙腈-甲酸-水溶液 (27∶10∶1∶62);体积流量 1.0 mL/min;检测波长为 286 nm;柱温 35 ℃。结果 双丹口服液中丹酚酸B的量逐月下降,而丹参素的量相对稳定。 结论 用丹酚酸B替代丹参素进行测定是不可行的。     

HPLC法测定复方降脂茶中丹酚酸B的含量

目的:建立保健品复方降脂茶中丹酚酸B的含量测定方法。方法:采用高效液相色谱法,ZORBAX SB-C_(18)柱(5μm,4.6×250mm),甲醇∶乙腈∶甲酸∶水(30∶10∶1∶59)为流动相,柱温30℃,286nm检测波长。结果:丹酚酸B在0.432~2.592μg,峰面积与丹酚酸B的质量呈良好线性关系(r=0.9992),平均回收率96.5%,RSD=1.43%(n=6)。结论:该方法简单、准确、重复性好,可作为该制剂的含量测定方法。     

通脉复方有效部位中丹酚酸B的含量测定

目的：建立高效液相色谱法测定通脉复方中丹酚酸B的质量分数。方法色谱柱为Diamonsil C18柱(4.6 mm×250 mm,5μm);流动相：甲醇-乙腈-甲酸-水(体积比30∶10∶1∶59);流速：1.0 mL·min-1;检测波长：286 nm;柱温：25℃。结果丹酚酸B在0.378~3.78μg范围内线性关系良好( r=0.9996);平均加样回收率为98.11%,RSD为1.2%( n=6)。结论本方法操作简便、重复性好,可用于通脉复方的质量控制。     

HPLC法测定金钱利胆丸中丹酚酸B的含量

目的:建立金钱利胆丸中丹酚酸B含量的测定方法。方法:采用反相高效液相色谱法,Kromasil ODS C18分析柱(5μm,250 mm×4.6 mm),流动相:甲醇-乙腈-甲酸-水(30∶10∶1∶59),检测波长286 nm,流速1.0 mL/min,柱温25℃。结果:丹酚酸B在0.126 8μg/mL～2.536μg/mL范围内线性关系良好(r=0.999 4),平均回收率为96.5%,RSD=1%(n=5)。结论:该法操作简便、准确,适用于金钱利胆丸的质量控制。     

反相高效液相色谱法测定解毒消银丸中丹酚酸B的含量

目的:建立解毒消银丸中丹酚酸B含量的RP-HPLC测定方法。方法:采用反相高效液相色谱法,C18色谱柱(4.6mm×250 mm,5μm);流动相:甲醇∶乙腈(3∶1)-1.0%甲酸水溶液(34∶66),流速:1 mL/min;柱温:30℃;检测波长:286 nm。结果:丹酚酸B在220 ng~4 440 ng范围内与峰面积呈良好线性,r=0.999 6,平均回收率为99.79%,RSD为1.79%。结论:该方法重现性好,准确度高,可用于解毒消银丸的质量控制。     

对复方丹参胶囊质量标准有关项目的改进和提高

目的：修订复方丹参胶囊中丹参酮IIA的鉴别项目,建立高效液相色谱法测定复方丹参胶囊中丹酚酸B的含量。方法：①鉴别硅胶G薄层板;点样量：10μL;展开剂：环己烷-醋酸乙酯（17∶3）;②含量测定：色谱柱：依利特Hypersil ODS色谱柱（5μm;250mm×4.6mm）;柱温：25℃;流动相：乙腈-甲醇-（甲酸-水）[体积比1∶35∶64（1：59）];检测波长：286nm。结果：供试品在与对照品色谱相应的位置上显相同的暗红色斑点,阴性无干扰;丹酚酸B在（0.122 9~0.737 3）μg范围内线性关系良好,回归方程：Y=1.098 3X-1.421 1,r=1.000 0;平均回收率为100.84%,RSD为1.88%。结论：此方法简便、灵敏、准确,可用于丹参酮IIA的鉴别和丹酚酸B的含量测定。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.