薯蓣皂苷元在口腔鳞癌中的抗癌作用研究

目的 探究薯蓣皂苷元对口腔鳞癌 HSC4 细胞生物学行为的影响。 方法 将 HSC4 细胞分为薯蓣 皂苷元 0、 0. 5、 1、 2 μmol / L 4 组, CCK-8、 5-溴-2′-脱氧尿苷 (BrdU) 染色、 克隆形成实验、 流式细胞术、 划痕实验和 Transwell 实验分别检测细胞增殖、 凋亡、 迁移和侵袭, 免疫印迹检测 Bcl-2、 Bax 和 cleaced caspase-3 蛋白表达水平; JC-1 检测线粒体膜电位; H2DCFDA 检测活性氧 (ROS) 产生; 试剂盒检测谷胱 甘肽 (GSH) 和丙二醛 (MDA) 水平。 结果 与薯蓣皂苷元 0 μmol / L 组比较, 薯蓣皂苷元 1 μmol / L 组和 2 μmol / L 组细胞活力、 集落形成能力、 BrdU 阳性细胞率、 迁移和侵袭能力、 线粒体膜电位降低, 细胞凋亡 率升高, Bax 和 cleaced caspase-3 蛋白表达上调, Bcl-2 蛋白表达下调, ROS 和 MDA 水平升高, GSH 水平降 低。 结论 薯蓣皂苷元可抑制 HSC4 细胞的增殖和运动能力, 通过线粒体途径和 ROS 的产生诱导细胞凋亡。     

介入治疗中晚期口腔颌面部鳞癌13例报告

目的探讨区域性动脉化疗口腔颌面部鳞癌的可行性及疗效。方法对13例病理诊断为口腔颌面部鳞癌的中晚期患者超选择性直接动脉灌注顺铂和阿霉素进行化疗,观察术中反应及术后疗效并随访,评价治疗的可行性及疗效。结果动脉化疗术中没有明显异常反映;动脉化疗的有效率为100.0%,肿瘤在化疗后当天或第2天,化疗局部疼痛和肿胀感缓解,口、鼻腔内出现分泌物或坏死组织脱落。13例中完全缓解(completeresponse,CR)5例,部分缓解(partialresponse,PR)8例。结论"介入动脉化疗"是治疗口腔颌面部鳞状细胞癌的有效手段之一,其疗效显著,避免手术之苦,可以作为综合序列治疗的重要组成部分。     

Visfatin Polymorphisms,Lifestyle Risk Factors and Risk of Oral Squamous Cell Carcinoma in a Cohort of Taiwanese Males

Oral cancer is the eighth greatest generally diagnosed cancer amongst males worldwide and the fourth most generally malignancy amongst Taiwanese males. The pro-inflammatory adipocytokine visfatin promotes tumor growth. Elevated plasma visfatin levels have been identified in patients with oral squamous cell carcinoma (OSCC), although the biological mechanisms underlying the involvement of visfatin in the pathogenesis of OSCC are not well understood. Moreover, no information is available regarding associations between visfatin polymorphisms and carcinogenic lifestyle factors with OSCC. This study, therefore, investigated the effects of four visfatin gene polymorphisms (rs11977021, rs61330082, rs2110385, and rs4730153) and carcinogenic lifestyle factors (betel nut chewing, alcohol consumption and cigarette smoking) on the risk of developing OSCC in 1,275 Taiwanese males with OSCC, and 1,195 healthy males (controls). We also examined the associations between these visfatin genotypes and OSCC histopathological prognostic factors (pathological stage, tumor status, lymph node status, and metastasis). We found that compared with subjects with the CC genotype of SNP rs11977021, those with the CT+TT genotype were less likely to progress OSCC. In addition, an association was found between the rs4730153 variant and lymph node metastasis in the OSCC cohort.     

右美托咪定对口腔鳞癌细胞生长和运动能力的调节

目的 探究右美托咪定 ( dexmedetomidine, DEX) 对口腔鳞癌 ( oral squamous cell carcinoma, OSCC) 细胞生长和运动的调节作用。 方法 人 OSCC 细胞 CAL27 分为 DEX0、 5、 10 及 20 nmol / L 组。 运用 克隆形成实验和 BrdU 实验评估细胞增殖; Transwell 法检测细胞侵袭; 流式细胞术检测细胞凋亡率; ELISA 检测超氧化物歧化酶 (SOD) 和丙二醛 (MDA) 水平; 微管形成实验评估细胞微管形成能力; Western 印 迹检测血管内皮生长因子 (VEGF)、 纤连蛋白 ( FN)、 B 细胞淋巴瘤 2 ( Bcl-2) 和 Bcl-2 相关 X 蛋白 (Bax) 的表达。 结果 与对照组比较, DEX 处理可显著降低 CAL27 细胞克隆形成率和 BrdU 阳性细胞率, 减少侵袭细胞数和微管结节数, 下调 VEGF 和 FN 表达水平。 同时 DEX 处理可促进氧化应激, 提高 Bax / Bcl-2 比率。 结论 DEX 处理可抑制 CAL27 细胞增殖、 侵袭和微管形成能力, 并促进其氧化应激和凋亡。     

口腔鳞癌患者血清催乳素水平的变化

研究口腔鳞癌患者血清催乳素(PRL)水平的变化.采用RIA检测了79名健康者和68例口腔鳞癌患者血清PRL水平.结果表明有38.2%(26/68)的患者血清PRL水平明显升高(P＜0.01);男女患者、各病变部位血清PRL水平之间无显著差异(P＞0.05).提示有部分口腔鳞癌患者存在高PRL血症且可能成为口腔鳞癌一个独立的预后因素.     

口腔鳞状细胞癌组织中细胞角蛋白19、乙酰肝素酶的表达及检测意义

目的 研究口腔鳞状细胞癌组织中细胞角蛋白19(cytokerantin-19,ck19)、乙酰肝素酶(heparinase,HPA)的表达及其检测价值,为临床治疗提供数据参考.方法 选取2013年1月至2014年1月期间在我院接受治疗的52例口腔鳞状细胞癌患者为研究对象,详细记录每位患者的各项资料,采集标本、检测ck19以及HPA的表达情况,分析其检测意义.结果 HPA的水平与患者年龄、性别无显著关系,与患者TNM分期、组织学分级、淋巴结是否转移明显相关;ck19的表达与患者的年龄、性别无关,与TNM分期以及组织学的分化存在明显的关联,P＜0.05;HPA诊断的灵敏度为94.0％ (47/50),特异度为50％ (1/2),ck19诊断的灵敏度为91.3％(42/46),特异度为33.3％ (2/6),对比两组结果,差异无统计学意义,P＞0.05.结论 ck19、HPA的表达与患者口腔鳞状细胞癌的发展有着明显的相关性,能够作为潜在的诊断标志物,具有较高的诊断价值.     

TRIM21 Polymorphisms are associated with Susceptibility and Clinical Status of Oral Squamous Cell Carcinoma patients

Squamous cell cancer of head and neck (HNSCC) is the sixth most common malignancy worldwide. One of the most common HNSCC types is oral squamous cell carcinoma (OSCC), which is the fifth leading cause of cancer death in Taiwan. Tripartite motif 21 (TRIM21) has been reported to play an important role in different cancer types. We found a correlation between TRIM21 and survival of HNSCC patients, but little information exists about how altered TRIM21 expression contributes to tumorigenesis. Thus, we investigated the combined effect of TRIM21 polymorphisms and exposure to environmental carcinogens on the susceptibility and clinicopathological characteristics of OSCC. Two single-nucleotide polymorphisms (SNPs) of TRIM21 (rs4144331, rs915956) from 1194 healthy controls and 1192 OSCC patients were analyzed by real-time PCR. Among 1632 smokers, TRIM21 polymorphism carriers with the betel-nut chewing habit had a ~4.8-fold greater risk of OSCC than TRIM21 wild-type carriers without the betel-nut chewing habit. After adjusting for other covariants, OSCC patients with G/T at TRIM21 rs4144331 had a high risk for distant metastasis compared with G/G homozygotes. This study is the first to examine the risk factors associated with TRIM21 SNPs in OSCC progression and development. Thus, our findings suggest that this study is the first to examine the risk factors associated with TRIM21 SNPs in OSCC progression and development and suggest that interactions between mutant genes may alter the susceptibility to OSCC.     

Cytoplasmic IGF2BP2 Protein Expression in Human Patients with Oral Squamous Cell Carcinoma: Prognostic and Clinical Implications

Oral squamous cell carcinoma (OSCC) is particularly prevalent in Taiwan. The goal of this study was to determine the clinicopathological role of insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) proteins as an indicator of clinical outcomes in OSCC patients. In this study, immunohistochemical (IHC) analysis was used to examine IGF2BP2 protein expression in 244 OSCC patients. We investigated the relationships among IGF2BP2 expression, clinicopathological variables, and patient survival. Our results showed that IGF2BP2 cytoplasmic protein expression was significantly correlated with lymph node metastasis, cancer stage, and patient survival. Kaplan-Meier survival curves revealed that elevated cytoplasmic IGF2BP2 expression levels in OSCC patients were associated with poor overall survival. Moreover, multivariate cox proportional hazard models revealed that cytoplasmic IGF2BP2 expression, T status, and lymph node metastasis were independent prognostic factors for survival. In conclusion, IGF2BP2 protein was found to be a helpful predictive marker for OSCC patients, as well as a possible therapeutic target for OSCC treatment.     

CD168 对口腔鳞状细胞癌增殖、 侵袭的作用机制研究

目的 探究 CD168 对口腔鳞状细胞癌增殖、 侵袭的作用机制。 方法 比较人正常口腔角质细胞 系 HOK 与不同口腔鳞癌细胞株间 CD168 表达差异, 选择 HN13 细胞株作为研究对象, 感染慢病毒 shRNA 载体后, 实时荧光定量 PCR (RT-qPCR) 和 Western 印迹法检测 CD168 基因和蛋白表达检测干预效果, CCK-8 法检测细胞增殖情况, 流式细胞仪检测细胞凋亡率, Transwell 试验检测细胞侵袭情况, Western 印迹 检测细胞增殖、 凋亡、 侵袭以及 CXCL12-CXCR4 / CXCR7 信号轴相关蛋白表达。 结果 选择 HN13 细胞和 CD168-shRNA2 慢病毒载体进行后续实验 (P< 0. 05); 沉默 CD168 可使 HN13 细胞增殖、 侵袭数量减少, 细胞凋亡率升高 (P< 0. 05), VEGF、 PCNA、 MMP-2、 MMP-9、 CXCL12、 CXCR4、 CXCR7 蛋白表达量降低 (P< 0. 05), Bax / Bcl-2 比值升高 (P< 0. 05), shRNA-NC 组变化无统计学意义 (P> 0. 05)。 结论 靶向沉 默 CD168 可抑制口腔鳞状癌细胞 HN13 增殖、 侵袭, 促进其凋亡, 可能与 CXCL12-CXCR4 / CXCR7 信号轴 有关。     

p63蛋白在食管磷状细胞癌中的表达及意义

目的检测p63蛋白在食管癌组织中的表达情况,并探讨其与食管癌临床病理特征的关系。方法采用免疫组化检测p63蛋白在53例食管磷癌及癌旁组织中的表达情况。结果p63蛋白在食管癌组织中的阳性表达率为71.7%（38/53）,而在癌旁食管组织中的阳性表达率为35.8%（19/53）,两者比较有显著性差异（P〈0.01）。p63蛋白的表达与食管磷癌分化程度密切相关（P〈0.05）,其在低分化磷癌中的阳性表达率（89.5%）显著高于在高分化磷癌中的阳性表达率（46.2%）。p63蛋白的表达与食管磷癌的TNM分期、淋巴结转移、浸润深度均无明显相关性（P〉0.05）。结论p63蛋白在食管磷癌组织中呈高表达,表明其可能参与食管磷癌的发生发展。     

VAV3蛋白在喉鳞状细胞癌中的表达及作用机制

目的 研究VAV3蛋白在喉鳞状细胞癌组织中的表达情况,及其对喉癌临床分型、淋巴转移等影响,分析其可能的机制.方法 利用免疫组织化学方法检查VAV3蛋白在喉鳞状细胞癌组织及喉部非癌组织中的表达,检测VAV3蛋白对喉鳞状细胞癌侵袭,淋巴转移等影响.结果 VAV3蛋白在喉鳞状细胞癌组织中表达为69.76％,在喉部非癌组织中的表达为10％,前者明显高于后者,差异有统计学意义(x2=11.937,P=0.001).它的表达与临床分型、T分型及淋巴结转移有统计学意义(P＜0.05),与性别、年龄、吸烟、分型之间无统计学意义(P＞0.05).结论 VAV3蛋白在喉癌组织中高表达,且可促进喉癌组织侵袭、转移能力.VAV3蛋白高表达,可作为判断喉癌及预后的一种新的分子标志.     

腺样鳞状细胞癌12例临床病理分析

１２例发生于外阴、阴茎、眼睑结膜、口腔、鼻腔、头部及下肢的腺样鳞癌病人中，男女各６人，年龄２４－７３岁，平均５８．４岁。肿瘤由角化鳞癌细胞形成的腺样结构和实性癌组织组成，均有广泛或较广泛的浸润，但有区域淋巴结转移者仅１例。局部扩大切除术治疗可有良好效果，此癌发生可能与普通鳞癌组织的变态性分化有关。     

口腔鳞癌细胞系HLAI类分子异常表达及其机制探讨

为探讨两个口腔鳞癌细胞系 (KB和Tca81 1 3)中HLAI类抗原的表达水平及其异常的分子机制。利用流式细胞术、Westernblot检测细胞系中HLAI类抗原在蛋白水平的表达 ;RT PCR检测细胞系在转录水平的表达。结果两个口腔鳞癌细胞系中HLAI类抗原蛋白水平的表达下调 ;RT PCR结果显示Tca81 1 3细胞系中A、B、C、LMP2、LMP7、LMP1 0基因的mRNA表达下调 ;在KB细胞系中除了A、B、C、LMP7、LMP1 0基因的mRNA表达下调 ,LMP2、PA2 8α基因的mRNA表达显著减少或缺失 ;而TAP1、TAP2、Tapasin基因在mRNA水平的表达无改变。IFN γ诱导后纠正了多个基因在mRNA水平的异常表达。两个口腔鳞癌细胞系中HLAI类抗原表达下调 ,且多基因在转录水平的异常也即转录效率的低下很可能是该下调的重要原因     

SLC7A11-AS1调控miR-429对口腔鳞癌细胞CAL-27细胞增殖、凋亡及迁移的影响

目的 探讨SLC7A11-AS1对口腔鳞癌细胞CAL-27细胞增殖、 凋亡及迁移的影响及分子机制.方法 收集29例口腔鳞癌患者癌组织及癌旁组织;CAL-27细胞分为si-NC组、si-SLC7A11-AS1组、si-SLC7A11-AS1+anti-miR-NC组、si-SLC7A11-AS1+anti-miR-429...     

食管鳞癌组织中miRNAs差异表达谱的筛选及研究

目的 筛选并鉴定在食管鳞癌（ESCC）及癌旁组织中miRNAs的差异表达,为进一步阐明其在ESCC发病机制中的作用奠定基础。方法 选取在邯郸市第一医院行食管癌切除术患者新鲜的鳞癌组织及癌旁正常组织,各3例,抽提总RNA,利用miRNAs芯片筛选其中差异表达的miRNAs,并对miR-106b-3p通过进一步RT-qPCR 技术进行验证。结果 miRNAs芯片从配对组织中共筛检出62个差异表达的miRNAs,其中41个miRNAs表达上调,21个miRNAs表达下调,鳞癌组织与癌旁正常组织中差异表达的miRNAs比较,差异有统计学意义（P＜0.05）。miR-106b-3p在ESCC组织中的表达高于癌旁组织,差异有统计学意义（P＜0.05）,与芯片结果一致。结论 食管鳞癌组织中miRNAs的差异表达为进一步研究miRNAs在ESCC发病中的作用奠定了基础;miR-106b-3p的高表达可能与ESCC的发生、发展有关。     

Papillary Renal Cell Carcinoma

Papillary renal cell carcinoma is an uncommon variant of renal cell carcinoma which has unique features including hypovascularity or avascularity, extensive stromal macrophage infiltration and better prognosis than that for nonpapillary renal cell carcinoma. Two cases of papillary renal cell carcinoma presenting hypovascular or avascular angiology are presented. Histologically, the two tumors had a purely papillary structure. Papillae were lined by a layer of epithelial cells which lacked prominent cellular atypia, and there were numerous macrophages in the stroma. In addition, in one patient, extensive calcification of the tumor capsule was present. Furthermore, our experience in the present study with imprint cytology indicates that it offers corroborative information for the intraoperative diagnosis made on the basis of frozen section examination. Acta Pathol Jpn 42: 298 303, 1992.     

头颈部鳞状细胞癌预后相关的miRNAs的生物信息学分析

目的 利用癌症基因组图谱（TCGA）数据库微小核糖核酸（miRNAs）表达谱数据分析头颈部鳞状细胞癌（HNSCC）与癌旁正常组织间差异表达的miRNAs,结合临床信息寻找与HNSCC预后相关的miRNAs。方法 从TCGA中下载miRNAs表达数据,包括39例HNSCC患者和39个肿瘤邻近正常组织样本筛选差异表达的miRNAs,应用481例HNSCC患者的miRNAs表达谱和临床信息来评估找到的差异表达miRNAs的预后作用。结果 共筛选出114个差异表达的miRNAs,包括60个上调和54个下调的miRNAs。Kaplan-Meier生存分析显示miR-4652-5p和miR-99a-3p与HNSCC患者预后相关,单因素和多因素Cox回归分析显示,miR-4652-5p和miR-99a-3p是HNSCC的重要预后因素。结论 miR-4652-5p和miR-99a-3p与HNSCC患者预后相关,但miR-4652-5p和miR-99a-3p在头颈鳞状细胞癌发生发展中的分子机制仍需更全面的基础和临床研究进行探讨。     

Papillary squamous cell carcinoma of the mandibular gingiva

Papillary squamous cell carcinoma (PSCC) has rarely been reported in the oral cavity. Herein reported is a case of PSCC in the mandibular gum. A 70-year-old man consulted our hospital because of a papillary tumor in the left mandibular gum. Physical examination revealed an exophytic papillary tumor of the left mandibular gum, and an excision of the tumor was performed. Grossly, the tumor was exophytic and papillary, and measured 1 x 1 x 0.8 cm. Microscopically, the tumor showed exophytic papillary proliferation with fibrovascular cores and consisted of atypical squamous epithelial cells. The tumor cells showed hyperchromasia, nuclear atypia, mitotic figures, apoptotic bodies, cancer pearls, and individual keratinization. Mild stromal invasion was seen. Immunohistochemically, the tumor cells were positive for pancytokeratin AE1/3, pancytokeratin CAM5.2, p63, p53, and Ki-67 (labeling index=40%), but negative for human papilloma virus (HPV). HPV in situ hybridization revealed no signals. Therefore, PSCC was diagnosed. The lateral and vertical margins are negative for tumor cell. The pathological diagnosis was PSCC. The patient was healthy and free from tumor three months after the operation.     

口腔鳞癌组织中P15和P21的表达研究

目的探讨p15和p21基因的蛋白产物在口腔鳞癌中的表达及意义。方法采用免疫组化P—V法检测108例口腔鳞癌和10例正常口腔黏膜组织中P15和P21蛋白的表达，并将结果进行统计分析。结果口腔鳞癌组织中P15蛋白的阳性表达率低于正常口腔黏膜组织（P〈0．05），而P21蛋白的阳性表达率两组间无明显差异（P〉0．05）；口腔鳞癌中，Ⅲ、Ⅳ期口腔鳞癌P15蛋白的阳性表达率明显低于Ⅰ、Ⅱ期口腔鳞癌的表达率（P〈0．01），无颈淋巴结转移组P15蛋白表达水平比有转移组高（P〈0．05）；P21阳性表达率在不同性别组中的表达差异具有统计学意义（P〈0．05）。结论P15蛋白的表达与口腔鳞癌的临床分期及淋巴结转移有关，P15蛋白表达水平可作为临床评估口腔鳞癌恶性程度、转移和预后的参考指标。