共查询到19条相似文献,搜索用时 15 毫秒
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Kucukkaya Eren Selen Bahador Zırh Elham Zeybek Naciye Dilara Askerbeyli Örs Sevinc Aksel Hacer Parashos Peter 《Odontology / the Society of the Nippon Dental University》2021,109(2):313-320
Odontology - To investigate the effect of benzalkonium chloride (BAC) addition to ethylenediaminetetraacetic acid (EDTA) on transforming growth factor-β1 (TGF-β1) release, as well as... 相似文献
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Richard J. Miron Nikola Saulacic Daniel Buser Tateyuki Iizuka Anton Sculean 《Clinical oral investigations》2013,17(3):981-988
Objectives
Bioresorbable collagen membranes are routinely utilized in guided bone regeneration to selectively direct the growth and repopulation of bone cells in areas of insufficient volume. However, the exact nature by which alveolar osteoblasts react to barrier membranes as well as the effects following the addition of growth factors to the membranes are still poorly understood. The objective of the present study was therefore to investigate the effect of a bioresorbable collagen membrane soak-loaded in growth factors bone morphogenetic protein 2 (BMP2) or transforming growth factor β1 (TGFβ1) on osteoblast adhesion, proliferation, and differentiation.Material and methods
Prior to experimental seeding, membranes were soaked in either BMP2 or TGFβ1 at a concentration of 10 ng/ml for 5 min.Results
Human osteoblasts adhered to all soak-loaded membranes as assessed by scanning electron microscopy. Growth factors BMP2 and TGFβ1 increased osteoblast proliferation at 3 or 5 days post-seeding when compared to control collagen membranes. Analysis of real-time PCR revealed that administration of BMP2 increased osteoblast differentiation markers such as osterix, collagen I, and osteocalcin. BMP2 also increased mineralization of primary osteoblasts as demonstrated by alizarin red staining when compared to control and TGFβ1 soak-loaded membranes.Conclusion
The combination of a collagen barrier membrane with growth factors TGFβ1 and BMP2 significantly influenced adhesion, proliferation, and differentiation of primary human osteoblasts.Clinical relevance
The described in vitro effects following the combination of collagen barrier membranes with growth factors TGFβ1 and BMP2 provide further biologic support for the clinical application of this treatment strategy in guided bone regeneration procedures. 相似文献9.
Li Zhang Lei Cheng Yujia Cui Zuping Wu Linyi Cai Liu Yang Mengmeng Duan Demao Zhang Chenchen Zhou Jing Xie 《Journal of periodontology》2021,92(11):e103-e115
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Objective. This study is to identify the expression of integrin β1 in human dental pulp cells and the role of integrin β1 in pulp cell adhesion on extracellular matrix protein laminin and fibronectin.Study design. Immunoblot detection of integrin β1 in human dental pulp cells was with the use of monoclonal anti-β1 antibody. Dental pulp cell adhesion assay on extracellular matrix protein laminin and fibronectin and blocking cell adhesion was performed with monoclonal anti-β1 antibody.Result. Integrin β1 was identified in human dental pulp cells. Pulp cells adhered and spread on both laminin and fibronectin. Monoclonal anti-β1 antibody inhibited human dental pulp cells adhesion on laminin but not on fibronectin.Conclusions. Integrin β1 was expressed on human dental pulp cells and mediated cell adhesion on laminin. Human dental pulp cells also adhered on fibronectin but the adhesion was not regulated by β1 integrin. 相似文献
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Mononuclear cells play an important role in the modulation of healing. The characteristics of implant surface topography may alter the production of signaling molecules such as cytokines. The aim of this in vitro study was to evaluate the effects of commercially available titanium surface treatments on both cell viability and the secretion of the antagonist cytokines, IL1β and TGFβ1. Human mononuclear cells were cultured on 10 mm diameter commercially pure titanium (cpTi) disks that were prepared using a turning procedure (control = machined surface) and either acid etched or bio-anodized for 1-7 days. Adhered cells were investigated with respect to cell viability using an MTT assay, and cytokine production was verified using an ELISA assay. The results indicate that surface characteristics did not alter the cell viability at days 1 and 4, although the machined surface presented the highest absorbance values at day 7 (p = 0.0084). Cell viability was reduced throughout the time course for all analyzed surfaces (p < 0.05). On day 4, IL1β levels were significantly higher on bio-anodized compared to acid etched surfaces (p = 0.0097). TGFβ1 did not show differences among the surfaces at days 1 and 4. The responses of non-stimulated mononuclear cells to titanium surfaces suggest only modest effects of the surface treatment and roughness on pro-inflammatory cytokine (IL1β) release. 相似文献
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This work reports the behaviour of osteoblastic human alveolar bone cells (first subculture) in the presence of chorhexidine
(CHX) and povidone–iodine (PI). Short contact (2 min) of 24-h cultures with CHX, at 0.12 and 0.2%, and PI, at 5 and 10%, caused
cell death within minutes; contact with 1% PI resulted in loss of the elongated characteristic cell shape. Cell adhesion was
adversely affected at concentrations higher than 5 × 10−5% CHX or 0.05% PI. Long-term exposure to CHX at 10−5 and 10−4% or PI at 10−4% had little effect on cell growth and caused an induction in the synthesis of alkaline phosphatase (ALP). Concentrations
of CHX and PI similar and higher than, respectively, 5 × 10−4% or 0.05% caused dose-dependent deleterious effects. CHX affected mainly the cell growth, whereas the effects of PI were
observed mostly in ALP production and matrix mineralization. Considering the levels of CHX and PI used routinely in the oral
cavity, results suggest that CHX has a higher cytotoxicity profile than PI. This observation might have some clinical relevance
regarding the potential utility of PI in the prevention of alveolar osteitis. 相似文献
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Teare JA, Petit J‐C, Ripamonti U. Synergistic induction of periodontal tissue regeneration by binary application of human osteogenic protein‐1 and human transforming growth factor‐β 3 in Class II furcation defects of Papio ursinus. J Periodont Res 2012; 47: 336–344. © 2011 John Wiley & Sons A/S Background and Objective: Binary applications of recombinant human osteogenic protein‐1 (hOP‐1) and transforming growth factor‐β3 (hTGF‐β3) synergize to induce pronounced bone formation. To induce periodontal tissue regeneration, binary applications of hOP‐1 and hTGF‐β3 were implanted in Class II furcation defects of the Chacma baboon, Papio ursinus. Material and Methods: Defects were created bilaterally in the furcation of the first and second mandibular molars of three adult baboons. Single applications of 25 μg hOP‐1 and 75 μg hTGF‐β3 in Matrigel® matrix were compared with 20:1 binary applications, i.e. 25 μg hOP‐1 and 1.25 μg hTGF‐β3. Morcellated fragments of autogenous rectus abdominis striated muscle were added to binary applications. Sixty days after implantation, the animals were killed and the operated tissues harvested en bloc. Undecalcified sections were studied by light microscopy, and regenerated tissue was assessed by measuring volume and height of newly formed alveolar bone and cementum. Results: The hOP‐1 and hTGF‐β3 induced periodontal tissue regeneration and cementogenesis. Qualitative morphological analysis of binary applications showed clear evidence for considerable periodontal tissue regeneration. Quantitatively, the differences in the histomorphometric values did not reach statistical significance for the group size chosen for this primate study. The addition of morcellated muscle fragments did not enhance tissue regeneration. Binary applications showed rapid expansion of the newly formed bone against the root surfaces following fibrovascular tissue induction in the centre of the treated defects. Conclusion: Binary applications of hOP‐1and hTGF‐β3 in Matrigel® matrix in Class II furcation defects of P. ursinus induced substantial periodontal tissue regeneration, which was tempered, however, by the anatomy of the furcation defect model, which does not allow for the rapid growth and expansion of the synergistic induction of bone formation, particularly when additionally treated with responding myoblastic stem cells. 相似文献
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Jing Chen Miao Yu Xiao Li Qin-Feng Sun Cheng-Zhe Yang Pi-Shan Yang 《Journal of periodontal research》2020,55(3):363-373
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Shaminea Sritharan Thirumulu Ponnuraj Kannan Mohd.Nor Norazmi Asma Abdullah Nurul 《Journal of cranio-maxillo-facial surgery》2018,46(8):1361-1367
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In this study, we evaluated the potential role of IL-6 and/or IL-17A in regulating the OPG/RANKL (osteoprotegerin/receptor activator of nuclear factor kappa b ligand) system of murine osteoblast cell line (MC3T3-E1) cultured on hydroxyapatite (HA).Methods
MC3T3-E1 cells were seeded on HA and treated with recombinant IL-6 or rIL-17A or combination of the two cytokines. Cell proliferation and differentiation activity were measured by MTS and alkaline phosphatase assays respectively. Observation of cell adhesion and proliferation was examined by scanning electron microscopy. Gene and protein expressions were performed on RANKL and OPG using qPCR, Western blot and ELISA.Results
We demonstrated that treatment with recombinant IL-17A (rIL-17A) and the combination rIL-6/rIL-17A promoted better adhesion and higher proliferation of cells on HA. Cells treated with rIL-17A and the combination cytokines showed a significant increase in differentiation activity on day 7, 10 and 14 as indicated by ALP activity (p < 0.001). Gene and protein expressions showed significant up-regulation of OPG and ALP (p < 0.001) and down-regulation of RANKL (p < 0.001) expression by all the treated groups. Interestingly, the combination of the two cytokines resulted in a significant increase of OPG/RANKL ratio (p < 0.001).Conclusion
These findings indicated that treatment with the combination of the two cytokines (IL-6/IL-17A) has synergistic effects to promote osteoblastic differentiation but suppress osteoclastogenesis by altering the OPG/RANKL ratio. 相似文献18.