厚蜂窝菌的化学成分研究

目的研究厚蜂窝菌发酵液的化学成分。方法厚蜂窝菌发酵液的乙酸乙酯提取部位经过硅胶、RP-18、Seph-adex LH-20等多种材料进行分离纯化,通过理化方法和波谱分析进行结构鉴定。结果分离鉴定了11个化合物,经波谱数据分析分别鉴定为角鲨烯(1)、9(Z)-十八烷烯酸(2)、麦角甾-4,6,8(14),22-四烯-3-酮(3)、麦角甾-5,7,22-三烯-3β-醇(4)、过氧麦角甾醇(5)、麦角甾-6β-甲氧基-7,22-二烯-3β,5α-二醇(6)、麦角甾-7,22-二烯-3β,5α,6β-三醇(7)、8,14-环氧麦角甾-4,22-二烯-3,6-二酮(8)、麦角甾-3β,5α,9α-三羟基-7,22-二烯-6-酮(9)、2,2-二甲基-1-苯并吡喃-6-醇(10)、2-呋喃甲酸(11)。结论以上化合物均是首次从该种真菌中分离得到。     

血党化学成分的研究

目的:研究血党Ardisia punctata的化学成分。方法:采用反复硅胶柱色谱和制备高效液相色谱进行分离纯化,根据理化性质和光谱数据进行结构鉴定。结果:得到12个化合物,鉴定为3-羟基-5-十三烷基-苯甲醚(1),5-十五烷基-1,3-间苯二酚(2),2-甲氧基-6-十三烷基-1,4-苯醌(3),2-甲氧基-6-十五烷基-1,4-苯醌(4),glutinol(5),ar-disicrenoside A(6),ardisiacrispin B(7),24-乙基-Δ7,22-胆甾二烯-3-酮(8),24-乙基-Δ7,22-胆甾二烯-3-醇(9),胡萝卜苷(10),香草酸(11),正三十四烷酸(12)。结论:所有化合物均为首次从该植物中获得。     

炮弹果果肉的化学成分研究

目的 研究炮弹果果肉的化学成分.方法 将炮弹果果肉用95％乙醇提取,通过硅胶、RP-18、Sephadex LH-20等色谱方法分离化合物,波谱方法鉴定化合物的结构.结果 从炮弹果果肉中分离得到18个化合物,ningpogenin (1)、6 -O-p-hydroxybenzoylaucubin (2)、3,3′-bisdemethylpinoresinol(3)、(22E,24R)-ergosta-7,22-dien-3β-ol (4)、麦角甾_4,6,8 (14),22-四烯-3-酮(5)、麦角甾-7,22-二烯-3β,5α,6β-三醇(6)、5α,8α-epidiory-(22E,24R)-ergosta-6,22-dien-3β-ol (7)、β-谷甾醇(8)、胡萝卜苷(9)、3β,5α,9α-三羟基-麦角甾-7,22-二烯-6-酮(10)、麦角甾-7,22-二烯-3-酮(11)、sesquiterpene (12)、对羟基苯甲酸(13)、苯甲酸(14)、对羟基苯乙醇(15)、对羟基苯甲醇(16)、D-阿洛醇(17)、5-hydroxymethyl-2-furancarboxaldehyde (18).结论 所有化合物都是首次从炮弹果果肉中分离得到.     

星宿菜化学成分的研究

目的：研究星宿菜95%乙醇提取物醋酸乙酯萃取部位中化学成分。方法：采用反复硅胶柱色谱分离化学成分,根据NMR数据和参考相关文献,鉴定化合物结构。结果：分离到9个化合物,分别为24-烯-环阿尔廷酮(1),24-乙基-Δ^7,22-胆甾二烯-3-酮(2),正三十五烷醇(3),β-豆甾醇(4),24-乙基-Δ^7,22-胆甾二烯-3β-醇(5),棕榈酸(6),异鼠李黄素(7),山柰酚(8)和槲皮素(9)。结论：化合物1～9均为首次从该植物中发现,化合物1,2,5为首次从该属植物中发现。     

云南美登木内生真菌Botryosphaeria sp. MHF次生代谢产物研究

目的:对云南美登木内生真菌Botryosphaeria sp.MHF的化学成分进行研究.方法:采用反相、正相等多种柱色谱法进行分离;应用波谱技术进行结构鉴定.结果:从云南美登木内生真菌B.sp.MHF的发酵物中分离得到8个化合物:分别是麦角甾-5-烯-3-醇(1)、麦角甾-4,6,8,22-四烯-3-酮(2)、麦角甾-3β,5α,9α-三羟基-7,22-二烯-6-酮(3)、麦角甾-7,22-二烯-3β,5α,6β-三醇(4)、麦角甾-5α,8α-环二氧-6,22-二烯-3-醇(5)、fusaproliferin (6)、脑苷脂C(7)和3,4,5-三羟基-四氢萘酮(8).结论:所有化合物均为首次从以Murashige-Skoog培养基培养的该菌株中分离得到.     

包疮叶的化学成分研究

目的：研究包疮叶的化学成分。方法：采用硅胶柱层析法进行分离，根据光谱数据鉴定结构。结果：从中分得6个化合物，分别为：22-O-(2-methylbutyryl)-28-al-12-en-olean-3β，16α-diol(1)，22-O-hexanoyl-28-al-12-en-olean-3β，l6α-diol(2)，l，12-bis(3，3′-dihydroxy-4，4′-dimethyl-5，5′-dimethoxypheny(l)dodecane(3)]，3β，16α，22α，28β-tetrahydroxy-13β,28-epoxy-olean(4)，(24R)-stigmast-7，22(E)-dien-3α-ol(5)，(24R)-stigmast-7，22(E)-dien-3β-D-glucopyranoside(6)。结论：化合物1为一新的齐墩果烷型甙元，其它5个化合物均为首次从该植物中得到。     

Eupha-7,9(11),24-trien-3beta-ol ("antiquol C") and other triterpenes from Euphorbia antiquorum latex and their inhibitory effects on Epstein-Barr virus activation

The structures of three triterpene alcohols isolated from the latex of Euphorbia antiquorum were established to be eupha-7,9(11),24-trien-3beta-ol (2; antiquol C), 19(10-->9)abeo-8alpha,9beta,10alpha-eupha-5,24-dien-3beta-ol (3; antiquol B), and 24-methyltirucalla-8,24(24(1))-dien-3beta-ol (4; euphorbol) on the basis of spectroscopic methods. Compounds 3 and 4 have previously been assigned the erroneous structures of 10alpha-cucurbita-5,24-dien-3alpha-ol and 24-methyleupha-8,24(24(1))-dien-3beta-ol, respectively. Compounds 2-4 and four other known compounds isolated from the latex, euphol (1), lemmaphylla-7,21-dien-3beta-ol (5), isohelianol (6), and camelliol C (7), showed potent inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA).     

Oculatol, oculatolide, and A-nor sterols from the sponge Haliclona oculata

Chemical investigation of the marine sponge Haliclona oculata resulted in the isolation of eight new compounds including oculatol (1) and oculatolide (2) and six unusual A-nor steroids, 2-ethoxycarbonyl-2beta-hydroxy-A-nor-ergosta-5,24(28)-dien-4-one (3), 2-ethoxycarbonyl-24-ethyl-2beta-hydroxy-A-nor-cholesta-5-en-4-one (4), 2-ethoxycarbonyl-2beta,7beta-dihydroxy-A-nor-ergosta-5,24(28)-dien-4-one (5), 2-ethoxycarbonyl-2beta,7beta-dihydroxy-A-nor-cholesta-5-en-4-one (6), 2-ethoxycarbonyl-2beta,7beta-dihydroxy-24-methyl-A-nor-cholesta-5,22(E)-dien-4-one (7), and 2-ethoxycarbonyl-2beta,7beta-dihydroxy-A-nor-cholesta-5,22(E)-dien-4-one (8), along with 16 known steroids and indole derivatives. Their structures were unambiguously determined on the basis of extensive spectroscopic analyses.     

红树林植物海漆中的三萜和甾体化合物

目的:对红树林植物海漆Excoecaria agallocha的化学成分进行研究。方法:采用多种色谱分析手段进行分离纯化,根据化合物的理化性质及波谱数据鉴定其结构。结果:从海漆甲醇提取物的石油醚相中分离、鉴定了6个三萜和3个甾体化合物,分别为蒲公英赛酮(1)、β-香树脂醇乙酸酯(2)、3-β[(2E,4E)-6-oxo-decad ienoy-loxy]-olean-12-ene(3)、蒲公英赛醇(4)、乙酰油酮酸(5)、cycloart-22-ene-3β,25-diol(6),β-sitostenone(7),(24R)-24-ethylcholesta-4,22-dien-3-one(8)和β-谷甾醇(9)。结论:化合物5~8为首次从海漆中分到。     

构菌发酵菌丝体化学成分的研究

目的研究构菌Flammulina velutipes发酵菌丝体的化学成分。方法硅胶柱色谱分离纯化,波谱法进行结构鉴定。结果分离得到2个甾体类化合物、1个倍半萜及2个多元醇类化合物,经理化及波谱数据分析鉴定为5α,8αepidioxy(22E,24R)ergost6,22dien3βol(1),ergosta4,6,8(14),22tetraen3one(2),sterpuricacid(3),甘露醇(mannitol)(4),核糖醇(ribitol)(5)。结论化合物2～5为首次从构菌发酵菌丝体中得到。     

反柄紫芝中2个新三萜类化合物

目的研究反柄紫芝Ganoderma cochlear子实体的化学成分。方法利用硅胶和凝胶柱色谱及半制备HPLC进行分离纯化,采用多种谱学技术(1D、2D NMR)并根据理化常数鉴定结构。结果分离并鉴定了5个化合物,分别为拱状灵芝素G(1)、拱状灵芝素H(2)、麦角甾醇(3)、5α,8α-环二氧-6,22-麦角甾二烯-3β-醇(4)和(22E,24R)-7,22-麦角甾二烯-3β-醇(5)。结论化合物1和2为新化合物,其余化合物均为首次从灵芝属真菌中分离得到。     

总合草苔虫的化学成分研究

对采自深圳大亚湾的总合草苔虫Bugula neritinaL.中化学成分进行研究,从石油醚层和正丁醇层分离得到7个化合物。利用波谱分析和文献对照的方法,确定它们的结构分别为胆甾醇(Ⅰ);胆甾-4-烯-3-酮(Ⅱ);胆甾醇肉豆蔻酸酯(Ⅲ);3,β5,α9α-三羟基-(22E,24R)-麦角甾-7,22-二烯-6-酮(Ⅳ);3,β5,α6β-三羟基-(22E,24R)-麦角甾-7,22-二烯(Ⅴ);尿嘧啶(Ⅵ);胸腺嘧啶(Ⅶ)。其中化合物Ⅱ-Ⅶ为首次从总合草苔虫中分离得到。     

Spiranoid withanolides from Jaborosa odonelliana

Six new spiranoid withanolides, (20R,22R,23S)-5alpha-chloro-6beta,12beta,17beta,22-tetrahydroxy-1-oxo-12,23-cycloergosta-2,24-dien-26,23-olide (2), (20R,22R,23S)-5beta,6beta-epoxy-12beta,17beta,22-trihydroxy-1-oxo-12,23-cycloergosta-2,24-dien-26,23-olide (3), (20R,22R,23S)-5beta,6beta-epoxy-4beta,12beta,17beta,22-tetrahydroxy-1-oxo-12,23-cycloergosta-2,24-dien-26,23-olide (4), (20R,22R,23S)-5alpha,6beta,12beta,17beta,22-pentahydroxy-1-oxo-12,23-cycloergosta-2,24-dien-26,23-olide (5), (20R,22R,23S)-6beta,12beta,17beta,22-tetrahydroxy-5alpha-methoxy-1-oxo-12,23-cycloergosta-2,24-dien-26,23-olide (6), and (20R,22R,23S)-6beta,12beta,17beta,22-tetrahydroxy-2alpha,5alpha-epidioxy-1-oxo-12,23-cycloergosta-3,24-dien-26,23-olide (7), were isolated from the leaves of Jaborosa odonelliana. Compounds 2-7 were characterized by a combination of spectroscopic methods (1D and 2D NMR, MS) and molecular modeling.     

New cytotoxic oxygenated fucosterols from the brown alga Turbinaria conoides

Fucosterol (1), 24xi-hydroperoxy-24-vinylcholesterol (2), 29-hydroperoxystigmasta-5,24(28)-dien-3beta-ol (3), 24-ethylcholesta-4,24(28)-dien-3-one (4), 24xi-hydroperoxy-24-ethylcholesta-4,28(29)-dien-3-one (5), 24-ethylcholesta-4,24(28)-dien-3,6-dione (6), 24xi-hydroperoxy-24-ethylcholesta-4,28(29)-dien-3,6-di one (7), 6beta-hydroxy-24-ethylcholesta-4,24(28)-dien-3-one (8), and 24xi-hydroperoxy-6beta-hydroxy-24-ethylcholesta-4,28(2 9)-dien-3-one (9) were isolated from the marine brown alga Turbinaria conoides. The structures of these compounds were established by spectral analysis. Isolated for the first time from a natural source, the oxygenated fucosterols 4-9 exhibit cytotoxicity against various cancer cell lines.     

New lanostane-type triterpenoids from Ganoderma applanatum

Four new lanostane-type triterpenes were isolated from the MeOH extract of the fruiting bodies of Ganoderma applanatum. Their structures were established as 3beta,7beta,20,23xi-tetrahydroxy-11,15-dioxolanosta-8-en-26-oic acid (1), 7beta,20,23xi-trihydroxy-3,11,15-trioxolanosta-8-en-26-oic acid (2), 7beta,23xi-dihydroxy-3,11,15-trioxolanosta-8,20E(22)-dien-26-oic acid (3), and 7beta-hydroxy-3,11,15,23-tetraoxolanosta-8,20E(22)-dien-26-oic acid methyl ester (4), respectively, by extensive spectroscopic analyses.     

紫芝的化学成分研究

目的:研究紫芝子实体的化学成分。方法:采用硅胶、凝胶色谱法进行分离纯化,波谱法进行结构鉴定。结果:从紫芝乙醇提取物的氯仿部分分离得到6个甾醇,1个脂肪酸和1个哌嗪二酮衍生物。结构鉴定为:麦角甾-7,22-二烯-3β-醇(1),麦角甾醇(2),6,9-环氧麦角甾-7,22-二烯-3β-醇(3),过氧麦角甾醇(4),麦角甾-7,22-二烯-3-酮(5),β-谷甾醇(6),α-羟基-二十四烷酸(7),cyc lo(D-Pro-D-Val)(8)。结论:化合物1~8均为首次从紫芝子实体中分离得到。     

Isolation and structure of ruprechstyril from Ruprechtia tangarana

Bioassay (P388 lymphocytic leukemia cell line and human cancer cell lines)-guided separation of an extract prepared from the stem bark and twigs of the previously uninvestigated Ruprechtia tangarana led to the isolation of a new isocarbostyril designated ruprechstyril (1), secalonic acid A (2), 2'-O-methylevernic acid (3), 3,3',4-tri-O-methylflavellagic acid (4), lichexanthone (5), methyl asterrate (6), and 3beta,22E,24S-stigmasta-5,22-dien-3-ol (7). Only secalonic acid A exhibited cancer cell and microbial growth inhibition. The structure of ruprechstyril (1) was determined by HRMS and 1D and 2D NMR spectra and confirmed by single-crystal X-ray analysis. The structures and absolute stereochemistry of five of the other compounds were also established by X-ray crystal structure determination.     

Cucurbitane-type triterpenoids from the stems of momordica charantia

Four new cucurbitane-type triterpenes, cucurbita-5,23(E)-diene-3beta,7beta,25-triol (1), 3beta-acetoxy-7beta-methoxycucurbita-5,23(E)-dien-25-ol (2), cucurbita-5(10),6,23(E)-triene-3beta,25-diol (5), and cucurbita-5,24-diene-3,7,23-trione (6), together with four known triterpenes, 3beta,25-dihydroxy-7beta-methoxycucurbita-5,23(E)-diene (3), 3beta-hydroxy-7beta,25-dimethoxycucurbita-5,23(E)-diene (4), 3beta,7beta,25-trihydroxycucurbita-5,23(E)-dien-19-al (7), and 25-methoxy-3beta,7beta-dihydroxycucurbita-5,23(E)-dien-19-al (8), were isolated from the methyl alcohol extract of the stems of Momordica charantia. The structures of the new compounds were elucidated by spectroscopic methods.     

Verticillane derivatives from Bursera suntui and Bursera kerberi

The stems of Bursera suntui afforded two new verticillane derivatives, (1S,3Z,7E,11S,12S)-(+)-verticilla-3,7-dien-12,20-diol (1) and (1S,3Z,7E,11S,12S)-(+)-verticilla-3,7-dien-12,20-diol 20-acetate (2), together with (1S,3E,7E,11R)-(+)-verticilla-3,7,12(18)-triene (3), (1R,3E,7E,11R,12Z)-(+)-verticilla-3,7,12-triene (4), (1R,7E,11Z)-(-)-verticilla-4(20),7,11-triene (5), and (1S,3E,7E,11S,12S)-(+)-verticilla-3,7-dien-12-ol (6). Compounds 3 and 4 are new enantiomerically pure natural products whose racemic mixtures, derived from synthetic approaches toward the taxane skeleton, were obtained previously. The stems of Bursera kerberi afforded the new (1S,3E,7E,11S,12R)-(+)-verticilla-3,7-dien-12-ol (7) together with 3-5. This is the first time that verticillane derivatives have been isolated from the genus Bursera. Their structures and stereochemistry were elucidated by 1D and 2D NMR data, including COSY, NOESY, HSQC, and HMBC experiments, while the absolute configuration was determined by comparison of the optical rotatory dispersion data with that of recently revised (1S,3E,7E,11S,12S)-(+)-verticilla-3,7-dien-12-ol (6), obtained from Sciadopitys verticillata, and those of (1R,3E,7E,11R,12R)-(-)-verticilla-3,7-dien-12-ol (8) and (1R,3E,7E,11R,12S)-(-)-verticilla-3,7-dien-12-ol (9), isolated from the liverwort Jackiella javanica. The conformational preferences of 1-7 were studied by molecular mechanics modeling employing the Monte Carlo protocol.     

Trypanocidal diarylheptanoids from Aframomum letestuianum

Three new diarylheptanoids, (4Z,6E)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-7-(4-hydroxyphenyl)hepta-4,6-dien-3-one, letestuianin A (1), (4Z,6E)-5-hydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-4,6-dien-3-one, letestuianin B (2), and 1,7-bis(4-hydroxyphenyl)heptan-3,5-dione, letestuianin C (3), as well as the known (4Z,6E)-5-hydroxy-1,7-bis(4-hydroxyphenyl)hepta-4,6-dien-3-one (5) were isolated from Aframomum letestuianum. The known flavonoids 3-acetoxy-5,7,4'-trihydroxyflavanone, 3-acetoxy-7-methoxy-5,4'-dihydroxyflavanone, 7-methoxy-3,5,4'-trihydroxyflavone, and 3,3',4',5,7-pentahydroxyflavan were also obtained from this plant. Their structures were determined using a combination of 1D and 2D NMR techniques. The four diarylheptanoids were tested for growth inhibitory activity in vitro versus bloodstream forms of African trypanosomes. IC(50) values in the range of 1-3 microg/mL were found for compounds 3 and 5.