检测受染旋毛虫猪血清中循环抗原对诊断和监测本病的研究

用双抗体夹心-ELISA法检测54头感染旋毛虫的猪血清,33头(61.1±6.7％)呈阳性反应。11头受染猪,于感染后不同时间检测CAg,有10头(90.9％)显示阳性,且绝大多数(8头,72.7％)于感染后3天即出现阳性反应,另于感染后6天和9天各有1头阳性。同时对比检测110头正常猪和两种其它寄生虫病猪,仅有两头(1.8％)感染弓形体的猪出现轻度交叉反应。对北京海淀区某屠宰场1987年自外地引进224头猪,进得流行病学调查,有18头(8.0±1.8％)CAg显示阳性反应,其中且有11头猪同时伴有抗体阳性;另检测陕西省商县50头猪,CAg皆为阴性。     

双抗体夹心-ELISA用于旋毛虫活动性感染的诊断及疗效考核

目的检测宿主血清中旋毛虫循环抗原 ( CAg)以诊断本病和考核药物疗效。方法双抗体夹心 -ELISA法。结果 94只实验感染旋毛虫小鼠 ,受染 3d后 ,即有 7%的小鼠显示阳性反应 ,受染 30 d,无论受染幼虫数目多寡 ( 50、1 50及 30 0条幼虫 /只 ) ,全部受染鼠 ( 1 0 0 % )均显示阳性反应。给予丙硫咪唑治疗后的第 1周 ,CAg的阳性率仍为 1 0 0 % ,但在治疗后第 2、3及 4周 ,则分别降为 60 %、2 0 %及 1 0 %。检测 61头感染旋毛虫猪血清中 CAg,4 0头 ( 65.6%± 6.1 % )呈阳性反应。 1 0 0头正常猪均为阴性 ,30头感染囊尾蚴和 30头感染弓形虫的猪 ,仅 1头感染弓形虫的猪出现轻度交叉反应。检测 36例旋毛虫病人血清中 CAg,2 6例 ( 72 .2 % )呈阳性反应。 50例正常人皆为阴性。 1 4 2例其他 9种寄生虫病人 ,仅 1例囊尾蚴病人出现轻度交叉反应。结论旋毛虫 CAg的检测具有早期诊断和考核药物疗效的价值。     

免疫层析试纸条检测实验感染猪肉汁抗旋毛虫抗体的研究

目的观察免疫层析试纸条对实验感染猪肉汁中抗旋毛虫抗体的检测效果。方法以胶体金标SPA和旋毛虫肌幼虫ES抗原制备了免疫层析试纸条。将30头家猪随机分为2组：实验感染组20头,每头经口感染5000条旋毛虫幼虫,正常对照组10头。感染后10w用试纸条进行血清及肉汁抗体检测,并与镜检法进行比较。结果应用试纸条检测旋毛虫幼虫感染的家猪血清及肉汁的抗体阳性率均为100%（20/20）,肌肉压片镜检法的幼虫检出率亦为100%（20/20）,每克腿肌虫荷为111～400条幼虫（平均为231.36条）。应用试纸条和镜检法对正常对照组肉汁及膈肌时均为阴性。感染猪肌肉-20℃保存8个月的肉汁抗体阳性率亦为100%（20/20）。结论试纸条检测肉汁中旋毛虫抗体可用于新鲜肉及冷冻猪肉中旋毛虫检疫的初筛。     

人唾液、尿液中弓形虫抗原抗体的检测

目的探讨用唾液、尿液替代血清进行弓形虫感染免疫学诊断的可行性。方法用ELISA法对血清进行弓形虫循环抗原(CAg)、IgM、IgG抗体的检测,取上述各项免疫学指标阳性的血清样本80例,并随机抽取3项指标均为阴性的血清样本102例,分别与其唾液和尿液标本在同一反应板上同步进行测定,比较其阳性和阴性符合率。结果(1)554份血清样本中,CAg、IgM和IgG3项的阳性率分别为4.51%(25/554)、3.61%(20/554)、6.32%(35/554)。(2)唾液中检出IgM抗体阳性16例,与血清的阳性符合率为80%(16/20),检测CAg、IgG抗体均为阴性。(3)检测尿液中的抗原抗体均无一例阳性。结论采用唾液标本检测弓形虫IgM抗体,在弓形虫感染的诊断及流行病学调查中均具有一定的应用前景。     

襄樊市猪旋毛虫和猪囊虫感染调查

我市于1985-1993年对屠宰的猪感染旋毛虫和猪囊虫情况进行了调查。按国家有关肉品卫生检验规程要求,对旋毛虫检查,取猪的膈肌角,用剪刀顺肌纤维在标本的正反两面取样,每头猪取24个肉样品,压片镜检,发现囊包为阳性(感染)。猪囊虫检查分别取心肌、臀肌、腰肌、咬肌四个部位标本,发现囊尾蚴为阳性(感染)。9年中,本市肉联厂共屠宰猪290294头,其中旋毛虫感染率为6.76%(19637/290294),其逐年调查情况见表1。表1　襄樊市猪旋毛虫感染率年份　　　　感染率(%)定基比*环比**19854.94(2389/48344)100　　19864.05(2309/56937)81.9881.98198712.25(1209…     

应用BA-Dot-ELISA检测旋毛虫血清抗体的研究

以旋毛虫肌幼虫层析抗原及国产BA试剂,建立了快速检验旋毛虫病的生物素亲和素酶免疫斑点法(BA-Dot-ELISA)。用此法和Dot-ELISA、PPA-ELISA同时检测15份旋毛虫病人血清,阳性率分别为100％、93.3％和93.3％,检测417份非旋毛虫病人血清,均为阴性。用三种方法检测30份实验感染猪阳性血清,BA-Dot-ELISA敏感性分别为PPA-ELISA和Dot-ELISA的7.13倍和2.70倍。本法与血吸虫病、囊虫病、弓形虫感染者无交叉反应。表明本法对旋毛虫有较高的特异性和敏感性,有助于低水平血清抗体旋毛虫病的检测,适于早期诊断,流行病学调查及动物的宰前检查。     

用抗牛丝虫抗体Dot—ELISA检测人体丝虫循环抗原的研究

应用抗牛丝虫抗体及抗马来丝虫抗体进行Dot-ELISA检测49例班氏丝虫微丝蚴(mf)阳性血清,CAg阳性率分别为89.7％及91.8％;36例班氏丝虫mf(—)血清,CAg阳性率分别为47.2％及50.0％;31例马来丝虫mf(+)血清,CAg阳性率分别为87.0％及93.5％,非流行区肠线虫感染44例及正常人血清40例全部阴性,华支睾吸虫感染血清70例,两种抗体检测CAg的假阳性率分别为8.5％及7.1％,囊虫病血清76例,假阳性率均为3.9％。     

应用特异单克隆抗体测定猪囊尾蚴病人血清和脑脊液中的循环抗原

我们应用特异的抗猪囊尾蚴抗原的单克隆抗体(CCy1),建立了单克隆抗体抑制性ELISA方法以测定囊尾蚴病患者的循环抗原(CAg)。测定灵敏度为ng水平。83例囊尾蚴病患者血清的CAg阳性率为71.1％。其范围为0.16-128μg/ml。对41例囊尾蚴病患者,同时测定了血清和脑脊液中的CAg。单项或两项阳性的总阳性率为90.2％。114例正常人血清、107例其它寄生虫病患者(包括30例包虫病患者)的血清及10例非寄生虫病患者的脑脊液的CAg量均为零。有23例囊尾蚴病患者治疗半年到1年后,有21例抗原浓度为零。另2例分别为0.64μg/ml和1.6μg/ml。表明测定CAg不仅可以确定活动性囊尾蚴感染,而且可考核疗效。     

组织切片间接免疫酶染色法诊断旋毛虫病的实验研究

以感染旋毛虫小鼠肌肉组织切片作抗原,用间接免疫过氧化物酶染色法检测64份感染旋毛虫小鼠血清抗体,阳性63份(98.4％)。10份正常小鼠血清和感染微小膜壳绦虫、日本血吸虫、猪蛔虫小鼠血清各10份,均阴性。结果表明,此法检测旋毛虫抗体具有较高的敏感性和特异性,可用于旋毛虫病的诊断和流行病学调查。     

提高绦囊虫病人循环抗原检出率及临床价值的探讨

一般在循环抗原(CAg)未过剩的情况下,CAg与抗体(Ab)形成免疫复合物,由于抗原上结合簇的占据,限制了CAg再与检测中所加样抗体的结合,而影响了CAg的检出。抗原抗体的结合力除了其本身特性外,还需适应的pH、离子强度和温度。提高pH或升高相当的温度,均促使抗原抗体复合物的解离反应,并使抗体结构受到破坏,消除Ab再与Ag结合的能力.因抗体(不耐热型)在70℃30min受到结构性破坏,故作者选择加热处理样品法,以提高绦囊虫病人CAg的检出率。     

The usefulness of ELISA test for early serological detection of Trichinella spp. infection in pigs

Trichinellosis is a parasitic zoonosis transmitted to humans through consumption of raw or undercooked meat from animals infected with nematodes of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease. The first step of the study was the optimization of a new ELISA method enabling an early and specific serological diagnosis of trichinellosis in pigs and wild boars using excretory-secretory (ES) antigens obtained from in vitro cultures of L1 T. spiralis. Serum samples were assayed for anti-T. spiralis IgG antibodies using the new ELISA protocol and a reference test--Standard manufactured by Institut Pourquier. The optimization involved the selection of suitable plates for antigen coating, dilution of sera and antibodies and their time of incubation. On the basis of the optimization a new ELISA procedure for the detection of IgG and IgM against T. spiralis was elaborated. Conventional, Iberian pigs and SPF (Specific Pathogen Free) pigs were infected with 200, 1000 and 20,000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies against excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project Trichiporse: "Safe pork and horse meat on EU markets: early and unbiased diagnostic tests for Trichinella". Field samples of conventional pigs (1474) and wild boars (1784) were obtained from slaughter houses in different parts of Poland. Pigs were examined for the presence of Trichinella spp. using the artificial digestion method. Only four pigs were naturally infected with T. spiralis, the remaining were Trichinella larvae free. ELISA was used to examine IgG levels against L1 T. spiralis in pig and wild boar sera. The usefulness of ELISA for anti-IgG detection in pigs is usually limited by the nature of the antigen. The antigens were prepared in different laboratories: in Germany--Ag ES L1 T. spiralis (N), Italy--Ag ES L1 T. spiralis (W)) and in Poland--Ag ES L1 T. spiralis. Cut-off values for ELISA along with the estimated sensitivity and specificity were calculated using different methods: S/P%, M+3SD and ROC (Receiver Operating Characteristic). In SPF and Iberian pigs inoculated with 200, 1000 and 20,000 L1 T. spiralis, specific antibodies were detected 40, 30 and 25 dpi, respectively, with the use of the Standard (reference test). The analysis of the two ELISA procedures demonstrated a high sensitivity and specificity for the newly elaborated test utilizing the Ag ES L1 T. spiralis. In conventional pigs infected with 20,000 L1 T. spiralis specific antibodies were detected from 20 dpi when employing the new protocol. Similar results for the Standard and new ELISA test were obtained for serum samples of conventional pigs infected with 200 and 1000 larvae, which became positive from 40 dpi and 30 dpi, respectively. The results showed that both: the Standard and new protocols were comparable, and based on this, the new test was applied for further research. Results obtained adopting the new protocol with three antigens showed that two of them: Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis are similar. The specific IgG antibodies for infective doses of 200 and 1000 larvae for these antigens were detectable 40 and 30 dpi respectively. In pigs infected with the highest dose of T. spiralis larvae IgG antibodies were detectable from 20 dpi when Ag ES L1 T. spiralis was used. These results strongly indicate that in examined pigs, the specific IgG response to T. spiralis infection is dose dependant. Of 1474 examined pig sera only 0.99% gave a positive signal against ES L1 T. spiralis antigen. Of 1784 examined wild boars sera only 0.68 % gave positive results using the new ELISA protocol. ELISA is a useful method for detecting specific IgG antibodies in pigs experimentally infected with different doses of T. spiralis and naturally infected pigs. In pigs the specific IgG response is dose dependant. The Ag ES L1 T. spiralis increases the specifity of the method and reduces false positive results. Simultaneous use of both methods: digestion and ELISA for the diagnosis of Trichinella in naturally infected pigs and wild boars may increase the chances of eliminating meat infected with T. spiralis larvae.     

The influence of the procedure of excretory-secretory L1 trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs

BACKGROUND: Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. MATERIAL AND METHODS: The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznaii and Boza Wola, that were examined by artificial digestion. RESULTS: In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 LI T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).     

Detection of Trichinella spiralis antigens in urine of men and animals

The practical inability to diagnose Trichinella spiralis antibodies in man before day 20 post infection (dpi) has stimulated interest in the development of immunodiagnostic test to detect circulating antigens. Our previous experience showed that soon after infection immune complexes as well as uncomplexed parasite antigens in sera of infected rats could be detected. To diagnose the presence of antigen in urine, double sandwich-capture ELISA was applied using a peroxidase-conjugated rabbit immunoglobulin to T. spiralis larval antigens. The plates were coated with metabolic (AES) or somatic (AS) larval antigens. Mice were infected with 500 T. spiralis larvae. The urine samples from experimentally infected mice taken from 1 to 41 dpi. and the urine samples from patients of the Clinical Hospital in Bia?ystok taken from 3 to 120 dpi were examined. Before testing, the urine samples were heated for 6 min. at 100 degrees C and centrifuged for 6 min. at 5000 g, supernatants were used in ELISA. The presence of T. spiralis antigens in mice urine samples was detected between 6-26 days post infection (dpi) using double sandwich-capture ELISA. All samples taken later were negative as samples taken from uninfected mice. 3 from 9 human urine samples taken 3-10 dpi were positive, the remaining samples taken 3-10 and 10-30 dpi showed values near to "cut-off". In both mice and human urine samples the higher level of antigens was detected in ELISA when somatic larval antigen was used. The T. spiralis antigens were present in urine of infected men and mice in the first phase of infection.     

旋毛虫感染兔唾液中抗旋毛虫IgG抗体水平

将28只日本大耳兔随机分为实验组(20只)和对照组(8只),实验组用旋毛虫脱囊幼虫经口灌胃日本大耳兔(3000条/只),对照组不做任何处理。采集感染前和感染后1～6周兔唾液和血清以及对照组兔唾液和血清。建立旋毛虫肌肉幼虫排泄分泌抗原(MLESA)为诊断抗原的间接ELISA,测定兔唾液和血清中抗旋毛虫IgG抗体。结果显示,感染后1～6周,唾液阳性率分别为10%、15%、40%、65%、85%和95%;血清阳性率分别为35%、50%、80%、90%、100%和100%。感染后1～3周,唾液阳性率与血清阳性率差异有统计学意义(χ2=3.58、5.23、6.67,P0.05),感染后4～6周,两者差异无统计学差异(χ2=0.12、1.03、1.03,P0.05)。提示在血清标本采集困难的情况下,MLESA的间接ELISA法检测唾液中抗旋毛虫IgG抗体可作为旋毛虫病免疫诊断的辅助方法。     

旋毛虫成虫排泄分泌抗原检测唾液中抗旋毛虫IgG抗体的研究

目的 评价旋毛虫(Trichinella spiralis)成虫排泄分泌抗原(adult worm excretory-secretory antigen,AWESA)作为诊断抗原检测旋毛虫感染日本大耳兔唾液中抗旋毛虫IgG抗体的可行性. 方法 建立旋毛虫感染日本大耳兔和对照组兔动物模型,采集感染前和感染后1～6周兔唾液和血清以及对照组兔唾液和血清.制备AWESA,建立AWESA作为诊断抗原的间接酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA),以市售旋毛虫IgG抗体检测试剂盒作为对照,测定感染前和感染后1～6周兔唾液和血清以及对照组兔唾液和血清中抗旋毛虫IgG抗体.AWESA和试剂盒测得的唾液A值和血清A值进行线性相关分析,AWESA和试剂盒测得的唾液阳性率和血清阳性率分别进行x2检验.结果 AWESA检测唾液和血清特异性IgG抗体阳性率依次为0、5%、20%、40%、60%、85%、90%,0、30%、60%、85%、95%、100%、100%,除感染后0、1、2周外其余各周唾液A值与血清A值呈显著线性相关(P＞0.05、P＞0.05、P＞0.05、P＜0.05、P＜0.05、P＜0.05、P＜0.05).市售旋毛虫IgG抗体检测试剂盒检测旋毛虫感染前和感染后兔唾液和血清中特异性IgG抗体阳性率依次为0、15%、20%、40%、55%、75%、90%,0、35%、60%、95%、95%、100%、100%,除0、1、3周外其余各周唾液A值与血清A值呈线性相关(P＞0.05、P＞0.05、P＜0.05、P＞0.05、P＜0.05、P＜0.05、P＜0.05). 结论 AWESA与市售试剂盒检测唾液和血清中抗旋毛虫IgG抗体的阳性率具有一致性.     

Comparison of three antigen preparations to detect Trichinellosis in live swine using IgG-ELISA

A swine infected with Trichinella spiralis is a source of transmission to human through consumption of raw or improperly cooked pork. Detection of larvae is suitable for carcasses, so that pigs in households or farms can be examined serologically for trichinellosis. This study compared antigens, crude (CAg), excretory-secretory (ESAg) and surface (SAg), for their potential use in IgG-ELISA. Serum samples were collected from 5 experimentally infected swine with T. spiralis (pTs), 147 positive cases of 9 other parasitic infections, 12 mixed infections of other parasites, and 35 normal controls. At the same 100% sensitivity, specificity of tests was in a range of 98-77%. ESAg was the best source of antigen with specificity of 98.3% at cut-off value of 0.439. False positives included coccidiasis (1/86) and mixed infections (2/39). For CAg, trichuriasis (2/11), coccidiasis (5/86), and mixed infections (8/39) gave cross-reactions and some of these samples had OD values far above cut-off value of 0.332. Cross-reactions of SAg were Oesophagostomum spp-like GI-nematode infection (1/1), unidentified GI-nematode infections (2/3), trichuriasis (5/11), coccidiasis (29/86) and mixed infections (4/39). Thus, ESAg has the highest potential in serodiagnosis, with antibody to T. spiralis in pigs being detected at the earliest 16 day post-infection. However, crude antigen demonstrated a good specificity at 91.8%, and this antigen has a potential to be used as a detection of choice for swine trichinellosis, but the antigen preparation must be improved for higher specificity.     

鼠类动物与人类旋毛虫感染关系的研究

目的初步探讨鼠类动物与人类旋毛虫感染的关系。方法在室内和野外生境捕获鼠类动物,鉴定种类,肌肉压片检查旋毛虫幼虫,ELISA测定血清旋毛虫特异性抗体。结果1.02%的鼠类动物查到旋毛虫幼虫,旋毛虫血清抗体阳性率为20.30%。其中家栖和野栖鼠类旋毛虫病原检查感染率与血清抗体阳性率分别是1.85%和24.49%,0和8.57%,差异均无显著性(P均<0.01〉。结论旋毛虫病流行区鼠类动物旋毛虫感染率较高,家栖鼠类旋毛虫感染率高于野栖鼠,与人类及家养动物感染相关。     

免疫层析试纸条诊断旋毛虫病的研究

目的建立一种能诊断人体旋毛虫病及动物旋毛虫感染的快速血清学方法。方法以胶体金标SPA和旋毛虫肌幼虫ES抗原制备免疫层析试纸条(immunochromatographic strip),对旋毛虫病及其它寄生虫病患者血清、旋毛虫及其它寄生虫感染的动物血清进行检测。结果试纸条检测旋毛虫病患者与旋毛虫感染的小鼠、大鼠、兔、猪血清的阳性率分别为100%(20/20)、97.87%(92/94)、100%(5/5)、100%(5/5)及100%(25/25),15min内肉眼可观察结果;其它寄生虫病(并殖吸虫病、血吸虫病、华支睾吸虫病、囊虫病及包虫病等)患者及正常人血清、其它寄生虫感染动物及正常动物血清均为阴性。试纸条和ELISA对100条幼虫感染小鼠血清检测的阳性率分别为91.3%(21/23)和95.7%(22/23)(χ2=0.36,P>0.05),两种方法对200～500条幼虫感染小鼠血清检测的阳性率均为100%(71/71)。试纸条对乡土旋毛虫、布氏旋毛虫、伪旋毛虫及纳氏旋毛虫感染小鼠血清检测的阳性率亦均为100%。试纸条在4℃可保存13个月,检测结果在室温可保存3个月。结论该试纸条可用于人体旋毛虫病和动物旋毛虫感染的快速血清学诊断,也可用于其它种旋毛虫感染的血清流行病学调查。     

旋毛虫在小鼠先天性传播的初步研究

目的?摇研究旋毛虫在小鼠的先天性传播并观察母鼠抗旋毛虫抗体对攻击感染的保护作用。 方法 将昆明小鼠分为受孕后感染组和感染后受孕组，子鼠出生后1 d内剖杀，检查旋毛虫幼虫；将正常母鼠所产子鼠由感染旋毛虫的母鼠喂养，21 d后宰杀，检查旋毛虫幼虫。用间接ELISA检测感染母鼠所产子鼠出生后不同时间的血清抗旋毛虫抗体，观察母鼠抗旋毛虫抗体对攻击感染的免疫保护。 结果 受孕后7 d感染旋毛虫的母鼠所产的6只子鼠中有2只感染旋毛虫；感染旋毛虫后8 d和22 d受孕雌鼠所产子鼠的感染率分别为20%（2/10）和25%(2/8)，从子鼠检获的旋毛虫均是未成囊的幼虫。交叉哺乳实验表明正常母鼠所产的30只子鼠未见旋毛虫感染。感染母鼠所产27只子鼠出生后1、7、24及40 d的血清抗体阳性率分别为100%、100%、77.8%及14.8%，子鼠出生后40 d攻击感染的减虫率为62.0%；感染母鼠所产子鼠血清被动转移小鼠的减虫率55.7%，与对照组比较差异有显著性（P<0.01）。 结论 旋毛虫在小鼠可经胎盘传播，母鼠的抗旋毛虫抗体对子鼠抗攻击感染可能具有部分保护作用。