Exposure to Aroclor‐1254 impairs spindle assembly during mouse oocyte maturation

Polychlorinated biphenyls (PCBs), as typical environmental estrogen disruptors, are a structurally‐related group of halogenated aromatic hydrocarbons that are composed of 209 isomers and present as a mixture in the environment. PCBs congener with different numbers and positions of chlorine atoms substituted on the biphenyl moiety. Aroclor‐1254 is a mixture of more than 60 PCB congeners. Previous studies have provided the evidence that PCBs have severe negative effects on reproductive functions, but the effects of PCBs on spindle assembly during mouse oocyte maturation in vitro have not been reported. In the present study, female ICR mouse immature oocytes were cultured in M2 medium with 1 and 10 μg mL^?1 Aroclor‐1254 separately in vitro. The percentage of germinal vesicle breakdown (GVBD) and the first polar body extrusion were recorded. The results showed no significant difference in the percentage of GVBD or the first polar body extrusion between control oocytes and Aroclor‐1254‐treated oocytes. Further studies showed that the normal localization of γ‐tubulin and Aurora‐A kinase was interfered and α‐tubulin assembling into spindle was affected when mouse oocytes were exposed to Aroclor‐1254. The length of spindle from 10 μg mL^?1 Aroclor‐1254‐treated oocytes was longer than that from control oocytes, and the spindle area in the Aroclor‐1254‐treated groups were decreased. Furthermore, the percentage of DNA damage in cumulus cells revealed an increase after exposed to Aroclor‐1254. These results will provide the important reference for the prevention of reproductive disorders caused by PCBs. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1652–1662, 2016.     

Tretinoin-loaded lipid-core nanocapsules decrease reactive oxygen species levels and improve bovine embryonic development during in vitro oocyte maturation

In vitro oocyte maturation (IVM) protocols can be improved by adding chemical supplements to the culture media. Tretinoin is considered an important retinoid in embryonic development and its association with lipid-core nanocapsules (TTN-LNC) represents an innovative way of improving its solubility, and chemical stability, and reducing its toxicity. The effects of supplementing IVM medium with TTN-LNC was evaluated by analyzing production of reactive oxygen species (ROS), S36-phosphorilated-p66Shc levels and caspase activity in early embryonic development, and expression of apoptosis and pluripotency genes in blastocysts. The lowest concentration tested (0.25 μM) of TTN-LNC generated higher blastocyst rate, lower ROS production and S36-p66Shc amount. Additionally, expression of BAX and SHC1 were lower in both non-encapsulated tretinoin (TTN) and TTN-LNC-treated groups. Nanoencapsulation allowed the use of smaller concentrations of tretinoin to supplement IVM medium thus reducing toxic effects related with its use, decreasing ROS levels and apoptose frequency, and improving the blastocyst rates.     

未刺激的未成熟卵体外培养在多囊卵巢综合征患者中的应用

目的探讨未刺激的未成熟卵体外培养成熟（IVM）治疗难治性多囊卵巢综合征（PCOS）患者的疗效。方法2005年10月至2006年10月本中心对50例难治性PCOS患者65个周期的IVM治疗。结果9个周期取消,取消率为13．85％;65个周期共获卵1338枚,平均每周期20．6枚,培养48h后总成熟率为68．5％。正常受精率为70．2％,卵裂率为91．1％,每穿刺周期和移植周期的临床妊娠率分别为37．5％和40．6％,28例临床妊娠中,6例多胎,多胎妊娠率为21．4％。结论未经刺激的IVM技术用于难治性PCOS是一种有效的方法,可获得与常规体外受精-胚胎移植（IVF-ET）相似的临床妊娠率。     

Chlorpyrifos and endosulfan affect buffalo oocyte maturation,fertilization, and embryo development in vitro directly and through cumulus cells

This study was undertaken to examine the effect of 10 different levels (0, 0.005, 0.01, 0.02, 0.05, 0.1, 0.5, 1.0, 2.0, and 4.0 μg/mL) of two pesticides (chlorpyrifos and endosulfan) on buffalo oocyte viability, maturation, fertilization, and developmental competences in vitro. Studies were conducted to test the development of oocytes cultured with pesticides during maturation, fertilization, and during different embryo development stages. We also conducted experiments to test the hypotheses that the effects of these pesticides are hormones and somatic cells mediated. We observed a dose dependant decline in viability and developmental competence rates of oocytes. Chlorpyrifos and endosulfan had a negative impact on oocytes at 0.02 and 0.1 μg/mL levels, respectively. These pesticides reduced the oocyte nuclear maturation by a direct effect on oocytes, cumulus cell‐mediated action, and by blocking the action of hormones. Chlorpyrifos was found to be more ovotoxic and embryotoxic than endosulfan. This study will provide information on dose‐response relationship and risk assessment in domestic buffaloes. © 2009 Wiley Periodicals, Inc. Environ Toxicol 26: 57–67, 2011.     

Effects of lindane on oocyte maturation and preimplantation embryonic development in the mouse

Lindane, an organochlorine insecticide, is suspected of preimplantation embryonic toxicity based on in vitro experiments with bovine and murine embryos. To verify this hypothesis in vivo we tested lindane for developmental alterations during early embryonic cleavage in the mouse. Two treatment schedules were tested: three daily doses of 15 or 25mg/kg b.w. lindane were orally administered to female mice either before mating or immediately after mating. Morphologic alterations (lysis or fragmentation of blastomeres, developmental arrest) of two-cell embryos and morulae were evaluated by inverted microscopy. In addition, cytologic abnormalities and cell proliferation delay, possibly induced during the first four cleavage cycles, were evaluated by fluorescent microscope analysis of the number and morphology of blastomere nuclei. A statistically significant increase of degenerating two-cell embryos was induced by exposure of preovulatory oocytes to the highest tested lindane dose. Early cleavage embryos exposed to the same dose showed a lower average number of blastomeres per morula, as well as a 40% reduction of the mitotic index with respect to matched controls. However, mean values in individual litters were variable and litter analysis did not show a lindane-related effect. One possible mechanism for the observed effects could be the recently demonstrated inhibitory action of lindane on gap junction-mediated cell communication between oocyte and cumulus cells. A comparison between human exposure levels and experimental doses based on measured and predicted blood concentrations suggests that there are ample margins of safety for human embryonic development at the present exposure levels.     

双酚A对大鼠卵泡体外生长和卵母细胞成熟的影响

目的观察双酚A(BPA)对大鼠卵泡体外生长发育及卵母细胞成熟的影响。方法采用大鼠卵泡体外长期培养方法,从12～14d龄的雌性大鼠卵巢中机械性分离腔前卵泡(140～170μm),隔天分别换一半含BPA 0,50,100和150μmol·L-1的培养液,连续培养10d。倒置相差显微镜下观察卵泡发育的形态,计算卵泡存活率、有腔卵泡形成率和卵丘-卵母细胞复合体(COC)排出率,测定卵泡直径,显微镜下观察卵泡的排卵情况以及卵母细胞成熟情况,计算生发泡(GV)、生发泡破裂(GVBD)和第一极体(PB)的形成率;分别于培养2,6和10d时采用磁性酶联免疫法测定培养基中雌二醇和孕酮的分泌量。结果正常对照组卵泡在10d培养过程中,大多数正常对照组卵泡都经历了腔前卵泡、有腔卵泡以及成熟卵泡阶段。与正常对照组相比,BPA 100和150μmol·L-1组的卵泡存活率、有腔卵泡形成率、COC排出率、GVBD率以及PB率均明显降低(P<0.05)。BPA 50μmol·L-1组培养10d时的卵泡直径以及培养6和10d时BPA100和150μmol·L-1组卵泡和卵母细胞的直径均明显降低(P<0.05);与正常对照组相比,BPA 100和150μmol·L-1组卵泡膜细胞和颗粒细胞的增殖受到明显抑制(P<0.05)。与正常对照组相比,BPA 100和150μmol·L-1组在培养6和10d时雌二醇和孕酮含量均显著减少(P<0.01)。结论 BPA 100和150μmol·L-1可抑制大鼠卵泡的生长和卵母细胞的成熟。     

Reproductive toxicity of Aroclor-1254: Effects on oocyte, spermatozoa, in vitro fertilization, and embryo development in the mouse

Polychlorinated biphenyls (PCBs) have been reported to adversely affect reproduction in laboratory and wild animals. The present study was undertaken to determine the toxic potential of Aroclor-1254 (A-1254) on in vitro fertilizing ability of oocytes and epididymal sperm and on preimplantation embryo development in the mouse. A-1254 was added to the IVF medium at concentrations of 0.01, 0.1, 1.0, and 10.0 μg/mL. Cumulus masses containing the oocytes were obtained from superovulated B6D2F1 mice and were placed in the culture medium containing A-1254 to which epididymal sperm, capacitated in a medium without A-1254, were added. The IVF rate was assessed 20 to 24 h after insesemination. A-1254 significantly reduced the mean percent ova fertilized even at 0.1 μg/mL. Incubation of the cumulus masses in various concentrations of A-1254 for 6 h, followed by insemination with sperm capacitated in the presence of A-1254, also significantly reduced the IVF rate. Capacitation of sperm in A-1254-containing medium, followed by co-culture with untreated oocytes, failed to affect the IVF rate. No significant effect on sperm motility was observed following exposure to 1 and 10 μg/mL of A-1254. Estradiol-17 β also reduced the IVF rate, however, the effect of A-1254 was more severe compared to the estradiol treatment. Furthermore, addition of A-1254 to the embryo culture medium was associated with a significant decrease in embryo growth at 48 h and 96 h. These results demonstrate adverse effects of A-1254 on oocytes, IVF, and embryonic development in the mouse.     

The effect of glucocorticoids on mouse oocyte in vitro maturation and subsequent fertilization and embryo development

Increased glucocorticoid levels, due to medical therapy or stress-related, may affect reproduction via the hypothalamus–pituitary-axis or directly at the oocyte level. We examined the effects of natural (corticosterone) or synthetic (dexamethasone) glucocorticoids on mouse oocyte maturation and underlying changes in extracellular signal-regulated kinase (ERK) phosphorylation patterns. Fertilization and progression up to the blastocyst stage were also evaluated. Oocytes were exposed to corticosterone or dexamethasone (0, 0.25, 2.5, 25 or 250 μM) for 17 h during in vitro maturation. After maturation, ERK-1/2 activation in oocytes was assessed by SDS–PAGE and immunoblotting, and fertilization and developmental capacity were examined in vitro. Corticosterone exposure during oocyte maturation significantly decreased progression to metaphase II, fertilization and embryo development at the highest concentration. Corticosterone caused a concentration-dependent inhibition of ERK-1/2 activation, with the highest concentration resulting in considerable inhibition of oocyte ERK-1/2 phosphorylation and no blastocyst development. In contrast, dexamethasone had no effect on maturation, fertilization and cleavage, and no effect was seen on ERK-1/2 phosphorylation. Based on these in vitro findings, high glucocorticoid levels may have consequences for subsequent development, although a short exposure to physiologic or stress-related glucocorticoid levels may not represent a hazard to meiosis progression of the oocyte.     

继发性不孕患者卵泡液25-（OH）D水平与IVF卵母细胞发育关系的研究#br#

目的　探讨继发性不孕患者卵泡液25-（OH）D水平与体外受精（IVF）卵母细胞发育及临床结局的关系。方法　选取继发性不孕且需行体外受精（IVF）的192例患者作为研究对象,根据继发性不孕原因分为4组：输卵管因素组58例、子宫内膜异位症组45例、多囊卵巢综合征组47例、卵巢储备不足组42例。根据卵泡液25-（OH）D水平聚类分析分为2组,A组121例,B组71例。采用酶联免疫吸附测定（ELISA）法检测卵泡液25-（OH）D水平;采用化学发光免疫分析法测定外周血激素水平,包括雌二醇、孕酮、黄体生成素（LH）、卵泡刺激素（FSH）;行阴道彩超测量卵泡数及卵泡直径;在培养第3天（D3）及第5天（D5）进行胚胎评估。分析A组和B组卵泡液中25-（OH）D水平与IVF卵母细胞发育（IVF正常受精率、D3优质胚胎形成率、D5优质囊胚形成率）及临床结局（临床妊娠率）的关系。结果　4个不同病因组间年龄、体质量指数（BMI）、雌二醇、孕酮、LH、FSH及卵泡液25-（OH）D水平比较差异无统计学意义。B组卵泡液25-（OH）D水平显著高于A组（P＜0.05）;B组HCG日大卵泡数及雌二醇、孕酮水平较A组显著升高（P＜0.05）;B组IVF正常受精率、D3优质胚胎形成率、D5优质囊胚形成率、临床妊娠率均显著高于A组（P＜0.05）。结论　在继发性不孕行IVF治疗的女性中,卵泡液25-（OH）D高水平更易产生大卵泡及优质胚胎,其临床妊娠率也较高。     

NADPH oxidase participates in the oxidative damage caused by fluoride in rat spermatozoa. Protective role of α‐tocopherol

Fluorosis, caused by drinking water contaminated with inorganic fluoride, is a public health problem in many areas around the world. The aim of this study was to evaluate oxidative stress in spermatozoa caused by fluoride and NADPH oxidase in relationship to fluoride. Four experimental groups of male Wistar rats were administered with deionized water, NaF, at a dose equivalent to 5 mg fluoride kg^?1 per 24 h, NaF plus 20 mg kg^?1 per 24 h α‐tocopherol, or α‐tocopherol alone for 60 days. We evaluated several spermatozoa parameters in the four groups: standard quality analysis, superoxide dismutase (SOD) activity, the generation of reactive oxygen species (ROS), NADPH oxidase activity, TBARS formation, ultrastructural analyses of spermatozoa using transmission electron microscopy and in vitro fertilization (IVF) capacity. After 60 days of treatment, urinary excretion of fluoride was not modified by α‐tocopherol. Spermatozoa from fluoride‐treated rats exhibited a significant increase in the generation of ROS, accompanied by a significant increase in NADPH oxidase activity. The increase in ROS generation was significantly diminished by diphenylene iodonium, an inhibitor of NADPH oxidase activity. In contrast, a decrease in the generation of ROS, an increase in SOD activity and the prevention of TBARS formation process were observed in spermatozoa of rats exposed to fluoride plus α‐tocopherol. Finally, α‐tocopherol treatment prevented the IVF incapacity observed in the spermatozoa from fluoride‐treated rats. These results suggest that NADPH oxidase participates in the oxidative stress damage caused by subchronic exposure to fluoride. Copyright © 2010 John Wiley & Sons, Ltd.     

Oxidative stress as a damage mechanism in porcine cumulus‐oocyte complexes exposed to malathion during in vitro maturation

Malathion is one of the most commonly used insecticides. Recent findings have demonstrated that it induces oxidative stress in somatic cells, but there are not enough studies that have demonstrated this effect in germ cells. Malathion impairs porcine oocyte viability and maturation, but studies have not shown how oxidative stress damages maturation and which biochemical mechanisms are affected in this process in cumulus‐oocyte complexes (COCs). The aims of the present study were to determine the amount of oxidative stress produced by malathion in porcine COCs matured in vitro, to define how biochemical mechanisms affect this process, and determine whether trolox can attenuate oxidative damage. Sublethal concentrations 0, 750, and 1000 µM were used to evaluate antioxidant enzyme expressions, reactive oxygen species (ROS production), protein oxidation, and lipid peroxidation, among other oxidation products. COCs viability and oocyte maturation decreased in a concentration‐dependent manner. Malathion increased Cu, Zn superoxide dismutase (SOD1), glutathione‐S‐transferase (GST), and glucose 6 phosphate dehydrogenase (G6PD) protein level and decreased glutathione peroxidase (GSH‐Px) and catalase (CAT) protein level. Species reactives of oxygen (ROS), protein oxidation and Thiobarbituric acid reactive substances (TBARS) levels increased in COCs exposed to the insecticide, but when COCs were pre‐treated with the trolox (50 µM) 30 min before and during malathion exposure, these parameters decreased down to control levels. This study showed that malathion has a detrimental effect on COCs during in vitro maturation, inducing oxidative stress, while trolox attenuated malathion toxicity by decreasing oxidative damage.     

正常形态前向运动精子总数对体外受精受精率的影响

目的：探讨正常形态前向运动精子总数对体外受精（ IVF）受精率的影响。方法选取第1次进行常规体外受精－胚胎移植周期治疗的108对不孕夫妇分为A组、B组和C组。 A组为正常形态前向运动精子总数0～0．4×106／mL,B组为正常形态前向运动精子总数0．4～1．8×106／mL,C组为正常形态前向运动精子总数＞1．8×106／mL。对取卵前半年内的平均正常形态前向运动精子总数和体外受精受精率的数据进行回顾性统计分析,分析正常形态前向运动精子总数和体外受精受精率之间的关系。结果 C组的体外受精受精率为91．02％显著高于A组60．38％和 B 组76．06％的体外受精受精率,差异有统计学意义（P＜0．01）。结论正常形态前向运动精子总数大于0．4×106时,可获得稳定的体外受精率。     

An investigation on the correlation between drug dissolution properties and the growth behaviour of granules in high shear mixer

Objectives The aim of this study was to investigate the correlation between the growth behaviour and in‐vitro dissolution rate of water‐insoluble drugs prepared with high‐shear wet granulation. Methods Granules containing nimodipine, microcrystalline cellulose, low‐substituted hydroxypropylcellulose and aqueous solution of hydroxypropylcellulose were prepared and the effects of independent process variables, including impeller speed and liquid‐to‐solid ratio were taken into consideration. The mean granule size, granule‐size distribution (GSD), porosity and surface properties were monitored at different kneading times to identify the granule‐growth mechanisms simultaneously. A computer‐based method was applied to simulate the dissolution behaviour of polydisperse granules based on the GSD data. Key findings The in‐vitro dissolution rate of drug was high for the early stages of granulation and sharply decreased when coalescence and consolidation of granules started, approaching a flat and low level when granules were sufficiently consolidated. The simulated dissolution results were in agreement with experimental observations and were significantly affected by the GSD, porosity and surface properties of granules during the granulation process. Moreover the GSD was directly related to the granule‐growth behaviour and mechanisms. Conclusions In general, it was concluded that the dissolution properties of nimodipine basically correlated with the growth behaviour of granules in a high‐shear mixer. The simulation method based on GSD can be used as a convenient and rapid way to predict the dissolution properties for formulation development and granulation optimization.     

胚胎移植技术对体外受精-胚胎移植后异位妊娠的影响

目的 探讨体外受精-胚胎移植(IVF-ET)后异位妊娠(EP)发生的风险.方法 对我院2005年1月至21307年6月接受IVF-ET的500例临床资料进行回顾性分析.结果 IVF-ET后EP发生率为7.07%,移植困难的胚胎移植(ET)女性EP的发生明显高于无移植困难妇女.超声引导与否及移植管放置位置(1.1～2.0)cm对EP的发生无明显影响(P>0.05).结论避免困难移植可有效减少EP的发生.     

Lactational fenvalerate exposure permanently impairs testicular development and spermatogenesis in mice

Fenvalerate, a widely used synthetic pyrethroid insecticide, has been associated with poor semen quality in human being. However, little is known about the effects of lactational fenvalerate exposure on testicular development and spermatogenesis. The purpose of the present study was to investigate the effects of maternal fenvalerate exposure during lactation on testicular development and spermatogenesis in male offspring. Maternal mice were administered with fenvalerate (60 mg/kg) by gavage daily from postnatal day (PND) 0 to PND21. Lactational fenvalerate exposure markedly decreased the absolute and relative weights of testes and increased the number of apoptotic cells in testes of pups at weaning. Histological examinations showed abnormal seminiferous tubules with large vacuoles or complete spermatogenic failure in testes of fenvalerate-treated mice at weaning. Additional experiment showed that lactational fenvalerate exposure markedly reduced mRNA and protein levels of testicular P450scc, a testosterone (T) synthesis enzyme. Consistent with down-regulation of testicular P450scc, the level of serum and testicular T at weaning was significantly decreased in pups whose mothers were exposed to fenvalerate during lactation. Although the expression of testicular P450scc and serum and testicular T in adulthood restored to control level, the decreased weight of testes and histological changes were irreversible. Importantly, the percentage of mature seminiferous tubules (stages VII and VIII) and the number of spermatozoa were obviously decreased in adult male mice whose mothers were exposed to fenvalerate during lactation. Taken together, these results suggest that lactational fenvalerate exposure permanently impairs testicular development and spermatogenesis.     

促性腺素释放素类似物联合体外受精治疗子宫内膜异位症不孕症

目的 :探讨不孕症合并子宫内膜异位症病人应用促性腺素释放素类似物 (GnRH A)联合体外受精 (IVF)的妊娠效果。方法 :5例子宫内膜异位症 ,其中 3例为子宫肌腺病。先使用GnRH A治疗3～ 6mo ,待月经来潮后采用促性腺激素超排卵治疗 ,卵泡成熟后采卵 ,常规体外受精 胚胎移植 (IVF ET)。结果 :发现排卵期血催乳素 (PRL)增高 ,治疗后 5例妊娠 ,住院保胎 ,3例足月妊娠分娩 ,2例流产。结论 :子宫内膜异位症合并不孕症应用GnRH A治疗 3～ 6mo后IVF效果较好。     

不同丙泊酚载药体系中游离药物浓度与体外溶血性的关系

考察了静脉注射用丙泊酚脂肪乳、聚氧乙烯蓖麻油胶束、微乳、混合胶束体系中游离药物浓度与体外溶血性的关联.用HPLC法测定游离丙泊酚的浓度,建立了UV法测定溶血百分率的方法.结果表明,载药脂肪乳、聚氧乙烯蓖麻油胶束、微乳、混合胶束体系透析后游离药物浓度(μg/ml)分别为19.54±0.6、33.5±2.8、107.3±3.9、151.1±13.1,溶血百分率(%)分别为4.6±0.4、20.0±0.4、55.7±4.0、89.O±O.8,而四者空白组的溶血百分率(%)则分别为1.5、6.1、0、31.在各载药体系中,由丙泊酚引起的溶血百分率在3.1%～58%范围内与游离药物浓度具有良好的线性关系(r=0.9650).     

1例体外受精-胚胎移植后先天性肺结核病报道并文献复习

目的：探讨体外受精-胚胎移植后妊娠分娩的新生儿发生先天性肺结核的临床特点及诊治策略。方法：分析1例体外受精-胚胎移植后妊娠分娩先天性肺结核的临床资料,并通过检索“体外受精-胚胎移植后新生儿先天性肺结核”相关文献,总结其临床特点和诊治方案。结果：患儿,女,系体外受精-胚胎移植术后妊娠35+2周剖宫产娩出,患儿生后29 d因“反复发热、气促7 d”入院,伴咳嗽、心率快、肝脏肿大表现。经血液及痰液病原微生物DNA高通量基因分别检测确诊为先天性肺结核,给予异烟肼、利福平、环丝氨酸、利奈唑胺抗结核治疗25 d后病情好转出院。检索后共纳入国内外相关文献12篇,包括18例患儿,结合本病例共纳入19例患儿。患儿起病年龄（37.4±28.2）d,常见起病临床表现依次为呼吸窘迫16例（84.2%）、发热9例（47.4%）、呼吸暂停6例（31.6%）、咳嗽5例（26.3%）,其中呼吸暂停均发生于出生胎龄<32 周患儿,发热和咳嗽主要发生在矫正胎龄>34 周患儿。17例（89.5%）患儿接受四联及以上药物治疗,治愈或明显好转16例（84.2%）。结论：体外受精-胚胎移植后妊娠分娩新生儿先天性肺结核起病晚,病情进展快,临床表现多样且不典型,诊断困难,易漏诊;体外受精-胚胎移植后妊娠分娩的新生儿生后晚期出现呼吸困难且抗感染治疗无效者,需警惕先天性肺结核,痰、血液病原微生物DNA高通量检测有助于病原诊断     

黄素化颗粒细胞survivin蛋白的表达与卵巢反应性的相关研究

目的探讨Survivin蛋白在人卵巢黄素化颗粒细胞表达与超排卵周期卵巢反应性的关系。方法收集32例行体外受精-胚胎移植（IVF-ET）患者的卵巢黄素化颗粒细胞,根据取卵时卵泡发育数不同将卵巢反应性分为高反应组（n=7）、中反应组（n=20）及低反应组（n=5）。应用流式细胞仪Annex-inV-PI双染法检测颗粒细胞凋亡;采用免疫细胞化学方法检测黄素化颗粒细胞Survivin蛋白的表达,分析它们与卵巢反应性的关系。结果黄素化颗粒细胞的凋亡率与HCG日E2水平呈负相关（r=0.637,P〈0.01）,高反应组、中反应组和低反应组颗粒细胞凋亡率分别为（8.8±2.7）%、（11.8±4.1）%及（19.4±5.8）%,3组比较差异有统计学意义（P〈0.01）;黄素化颗粒细胞Survivin蛋白的表达HCG日E2水平呈正相关（P〈0.01）,高反应组、中反应组和低反应组Survivin蛋白的表达分别为2.591±0.167、2.44±0.229及2.016±0.140,差异有统计学意义（P〈0.01）。结论黄素化颗粒细胞Survivin蛋白的表达水平可反映卵巢储备功能与超促排卵过程中卵巢反应性。