腹腔镜下远端胃癌根治与D2淋巴结清扫安全性及可行性分析

目的：探讨腹腔镜下远端胃癌根治与D2淋巴结清扫的效果及安全性。方法收集80例远端胃癌患者,40例行腹腔镜下远端胃癌根治术的患者为研究组,40例采用开腹手术治疗的患者为对照组,对比分析两组手术指标(切口长度、手术时间、术中出血)、术后恢复情况(排气时间、下床活动时间、镇痛次数、并发症)及肿瘤根治范围等,评价腹腔镜胃癌根治的效果和安全性。结果研究组手术时间显著长于对照组,但切口长度、术中出血量均明显少于对照组,差异有统计学意义( P<0．05)。两组肿瘤近端及远端切缘、淋巴结清扫数量比较,差异无统计学意义(P>0．05)。研究组术后镇痛次数、肛门排气时间、下床活动时间明显少于对照组,差异有统计学意(P<0．05);除肺部感染外,两组其他并发症发生率差异无统计学意义(P>0．05)。结论腹腔镜下远端胃癌根治与D2淋巴结清扫虽然手术时间较长,操作复杂,但能够达到开腹手术相同的胃癌切除标准和淋巴清扫范围,且术后恢复方面有明显优势,值得应用。     

全腹腔镜与腹腔镜辅助远端胃癌根治术的近期疗效对比分析

目的:比较全腹腔镜与腹腔镜辅助远端胃癌根治术治疗胃癌的近期治疗效果。方法:回顾性分析本院2015年4月-2016年3月收治的行腹腔镜远端胃癌根治术患者48例,所有患者均由同组医生手术,将其分为全腹腔镜组18例和腹腔镜辅助组30例,比较两组患者的手术时间、淋巴结清扫数目、术中出血量、切口长度、术后排气时间、疼痛评分、住院时间及并发症。结果:全腹腔镜组手术时间明显长于腹腔镜辅助组,比较差异有统计学意义(P0.05);全腹腔镜组术中出血量、切口长度及术后疼痛评分均低于腹腔镜辅助组,比较差异均有统计学意义(P0.05);两组淋巴结清扫数目、术后排气时间、术后住院时间及术后并发症比较,差异均无统计学意义(P0.05)。结论:全腹腔镜具有术中出血量少、术后疼痛评分低等优点,有助于患者术后的早期恢复,而且由于术中切口长度小,美容效果更好,患者更容易接受。因此,全腹腔镜远端胃癌根治术值得临床推广和应用。     

腹腔镜与开腹治疗进展期远端胃癌的疗效及安全性对比

目的比较通过腹腔镜辅助下与传统开腹施行进展期远端胃癌根治术的安全性及近、远期疗效。方法回顾性分析2010年1月至2015年2月安徽医科大学第二附属医院收治的远端胃癌患者107例临床资料,其中55例行腹腔镜辅助下远端胃癌根治术,52例行开腹远端胃癌根治术。比较两组手术安全性、术后恢复相关指标及术后1、2、3、5年生存率。结果两组患者均无手术死亡病例,腹腔镜组手术时间（282.7±43.6）min长于开腹组的（217.6±29.8min）,差异有统计学意义（P＜0.05）,切口长度、术中出血量、肛门排气时间、止痛剂注射次数、住院时间、术后C反应蛋白水平均低于开腹组（ P＜0.05）;腹腔镜组并发症发生率（7.27％）与开腹组（21.15％）相比差异有统计学意义（P＜0.05）;两组淋巴结清扫数目及1、2、3、5年生存率对比差异无统计学意义（ P＞0.05）。结论腹腔镜辅助下胃癌根治术比开腹手术耗时长,但能达到与开腹手术相同的淋巴结清扫范围,且具有安全性高、创伤小、失血少、术后恢复快等优点。     

腹腔镜辅助D2根治术与开腹手术治疗进展期胃癌的疗效对比研究

目的 对比应用腹腔镜技术与传统开腹治疗进展期胃癌的安全性及短期疗效.方法 选择铜陵市人民医院2009年1月至2015年12月进行手术治疗的273例进展期胃癌患者,根据手术方式分为两组,92例行腹腔镜下胃癌D2根治术为腔镜组,181例行开腹胃癌D2根治术为开腹组,对比分析两组患者术中及术后康复指标(术中出血量、手术时间、术后胃肠功能恢复时间、术后住院时间)、手术并发症率及手术效果指标(淋巴结清扫数目、肿瘤远近切缘距离).结果 腹腔镜组与开腹组相比,全胃切除手术时间、术后胃肠功能恢复时间、术后住院时间、术后并发症发生率的差异有统计学意义(P<0.05),远端胃切除时间、术中出血量、淋巴结清扫数目及肿瘤远近切缘距离的差异无统计学意义(P>0.05).结论 腹腔镜技术与开腹手术治疗进展期胃癌的短期疗效与手术风险无明显差异,腹腔镜手术具有术后恢复快、并发症少的优势.     

腹腔镜辅助与开腹远端胃癌根治术的临床对比研究

目的比较腹腔镜辅助与开腹远端胃癌根治术的手术效果。方法选取2009年1月～2011年1月我院行腹腔镜辅助远端胃癌根治术患者28例为研究对象,设立为腹腔镜组,另选择同期开腹手术病例28例作为对照组,比较两组的手术时间、术中出血量、平均术中清扫淋巴结数、住院时间、术后并发症发生率。结果腹腔镜组手术时间明显长于对照组（P〈0．05）,腹腔镜组平均术中出血量、平均住院时间、平均术中清扫淋巴结数、术后并发症发生率明显少于对照组（P〈0．05）。结论腹腔镜辅助远端胃癌根治术具有术中出血少、术后恢复快、并发症少等优点,值得推广和应用。     

腹腔镜右半结肠癌根治术54例临床分析

目的：探讨腹腔镜右半结肠癌根治术的可行性疗效。方法：回顾分析我院54例行腹腔镜右半结肠癌根治术患者及32例行开腹右半结肠癌根治术患者的临床资料,比较2组患者术中术后情况。结果：腹腔镜组手术时间大于开腹组,术中出血量,术后肛门排气时间、术后住院时间小于开腹组,差异有统计学意义（P＜0．05）,而淋巴结清扫数量、并发症例数与开腹组相比,差异无统计学意义（P＞0．05）。结论：腹腔镜右半结肠癌根治术安全可行,具有患者创伤小,术后康复快的优点,根治效果可达到开腹手术的水平。     

腹腔镜胃癌根治术治疗胃癌的疗效和安全性研究

目的探讨腹腔镜胃癌根治术治疗胃癌的疗效和安全性。方法选取该院2010年12月—2013年5月期间收治的96例胃癌患者,随机均分为对照组和实验组,对照组患者行传统开腹胃癌根治术,实验组患者行腹腔镜胃癌根治术。比较两组患者手术时间、术中出血量、切口长度、淋巴结清扫枚数、术后住院时间和并发症发生率的差异。结果实验组手术时间、术中出血量、切口长度、术后住院时间以及并发症发生率均显著低于对照组,两组比较差异有统计学意义（P〈0.05）。实验组与对照组淋巴结清扫数目差异不大,组间比较差异无统计学意义（P〉0.05）。结论腹腔镜胃癌根治术具有出血少、切口小、恢复快等优点,临床疗效优于传统开腹手术,值得临床推广。     

腹腔镜与开腹宫颈癌根治术的临床对比分析

目的探讨腹腔镜与传统开腹宫颈癌根治术的治疗效果。方法回顾分析30例Ⅰa～Ⅱb期行腹腔镜下宫颈癌根治术患者与同期33例开腹手术患者的临床资料。结果腹腔镜组与开腹组手术时间、术中出血量、术后肛门排气时间、下床活动时间、住院天数、术后伤口感染发生率等差异均有统计学意义（P〈0．01或P〈0．05）,而清扫淋巴结数目差异无统计学意义（P〉0．05）。结论腹腔镜下宫颈癌根治术是安全可行的,且具有手术切口小、创伤小、术后恢复快等优点。     

3D腹腔镜手术与开腹手术切除胃部胃肠间质瘤的疗效观察

【摘要】 目的 探讨3D腹腔镜手术与开腹手术切除治疗胃部胃肠间质瘤的疗效。方法 收集2014年3月~2017年3月来院就诊的胃肠道间质瘤患者100例,随机分为研究组与对照组,每组各50例。研究组采取3D腹腔镜手术治疗,对照组采取开腹手术治疗,观察对比两种手术方案的手术情况、术后恢复情况、血清IL 6与CRP水平以及疼痛评分。结果〓两组患者手术时间比较,差异无统计学意义（P＞005）;研究组患者排气时间、术中出血量、住院时间等与对照组比较均有明显减少（P＜005）;研究组患者恢复进食时间、恢复活动时间、胃管放置时间、引流管放置时间等与对照组比较均有显著减少（P＜005）;研究组并发症发生率均低于对照组（P＜005）;研究组血清IL 6与CRP水平以及疼痛评分均显著低于对照组（P＜005）。结论 3D腹腔镜手术治疗胃部胃肠间质瘤相对于传统的开腹手术疗效更显著,手术过程中出血量少,疼痛轻,且患者术后恢复快,预后情况更佳,可在临床推广应用。     

腹腔镜胃癌D2根治术在进展期胃癌中的应用探讨

目的探讨腹腔镜胃癌D2根治术在进展期胃癌中的近期疗效。方法选择自2010年1月~2013年12月我院收治的胃癌患者中开腹54例按照常规的开腹手术进行胃切除术(开腹组),而50例行腹腔镜胃癌D2根治术(腹腔镜组),记录并比较术中、术后情况及术后并发症及病死率情况。结果腹腔镜组术中出血量、手术时间、住院时间及术后胃肠功能恢复时间均短于开腹组(P<0.05),两组胃癌术后病死率比较,差异无统计学意义(P>0.05)。结论与传统开腹远端胃癌D2根治术相比,腹腔镜手术对于进展期胃癌的治疗能达到同样的根治性效果,与此同时其具有手术时间短、切口小、出血少、恢复快、安全可靠等优点,值得临床推广应用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.