Binding of EGF1 Domain Peptide in Coagulation Factor Ⅶ with Tissue Factor and Its Implications for the Triggering of Coagulation

The binding function of EGF1 domain peptide with tissue factor(TF)and its ability of triggering coagulation were explored.The TF expression model in vitro was established by lipopolysaccha-ride induction.The affinity of EGFP-EGF1 and TF expressing cells was analyzed by fluorescence microscopy and flow cytometry(FCM).The affinity of EGFP-EGF1 and rat soluble TF was quantitated by surface plasmon resonance(SPR).The ability of EGFP-EGF1 in triggering coagulation was tested by prothrombin time assay.The FCM res...     

Association of coagulation factor Ⅶ with the risk of myocardial infarction in the Chinese

Objective To elucidate the association of plasma factor Ⅶ coagulant activity (FⅦc) w it h the risk of myocardial infarction (MI) and to assess the influence of factor Ⅶ gene MspI polymorphism and lipid metabolism on FⅦc in the Chinese.Methods A total of 137 patients with angiographically confirmed MI and 125 healthy indiv iduals were evaluated retrospectively. Plasma FⅦc was measured by one-sta ge prothrombin time, and FⅦ genotype was determined after MspI digestion of polym erase chain reaction-amplified genomic DNA. Serum lipid levels were assessed b y routine methods.Results MI patients had significantly higher levels of FⅦc (119.5%±22.7% vs 99. 9% ±21.8%, P<0.01) and total serum cholesterol (5.80±1.06 mmol/L vs 5.5 3±1.08 mmol/L, P<0.05) than controls, but only FⅦc independently co rrelated with the risk of MI (OR=1.04, P<0.01). There were no significant di fferences in FⅦ genotype or allele frequency between patients and controls ( P>0.05). Subjects with the Gln353 allele were associated with significantly lower FⅦc levels than Arg353 homozygotes (99.7%±19.3% vs 111.4%±24.6% , P<0.05). Serum triglyceride was positively correlated with plasma FⅦc in both control (r=0.25, P<0.01) and case (r=0.87, P<0.01) gro ups, but this correlation was restricted to Arg/Arg genotype (r=0.68, P <0.01). A significant correlation of total serum cholesterol with FⅦc onl y appeared in Arg/Arg homozygotes (r=0.17, P<0.01).Conclusions Our findings support the role of plasma FⅦc as a risk factor for MI in Chin es e. Plasma triglyceride and FⅦ gene MspI polymorphism are two independent d eter minants of FⅦc. Assay of this polymorphism will be helpful in determining who will benefit most from lipid-lowing therapy.     

Intrastriatal Gene Transfer of Vascular Endothelial Growth Factor Rescues Dopaminergic Neurons in a Rat Parkinson's Disease Model

Parkinson's disease (PD) is a chronic progressivegerontic disease, which is characterized by the degeneration of dopaminergic neurons in substantia nigra. Efforhas been made to find effective treatment to arrest oslow down the degeneration of dopaminergic neuronsOnce regarded as an endothelial cell-specific factorvascular endothelial growth factor (VEGF), in recenyears, was unexpectedly found to be a critical player inneurodegeneration, and have direct neurotrophic andneuroprotective effects…     

Effects of Liandou Qingmai Recipe(连豆清脉方) on Endothelin-1,Nitric Oxide,Interleukin-6 and Interleukin-10 Levels in Patients with Coronary Heart Disease

Objective:To observe effects of Liandou Qingmai Recipe(连豆清脉方) on endothelin-1(ET-1),nitric oxide(NO),interleukin-6(IL-6) and IL-10 levels in patients with coronary heart disease.Methods:Total 101 cases with coronary heart disease were randomly divided into a treatment group(n=45) treated by Liandou Qingmai Recipe and a standard treatment group(control group,n=56),with a normal group of 16 health persons set up.Changes of ET-1,NO,IL-6 and IL-10 levels were measured before treatment and after treatment for two weeks.And the data were analyzed by SPSS 16.0 statistic software.Results:Before treatment,the levels of ET-1,IL-6 and IL-10 levels were significantly higher and NO was significantly lower in the patients with coronary heart disease than those in the normal group(90.7±12.7 ng/L vs 41.8±13.5 ng/L,9.17±0.18 ng/L vs 1.10±0.08 ng/L,1.94±0.26 ng/L vs 1.09±0.06 ng/L,and 92.2±17.7 μmol/L vs 124.5±27.2 μmol/L;all P<0.05),with no significant differences in the levels of ET-1,NO,IL-6 and IL-10 between the treatment group and the control group(P>0.05);After treatment,ET-1 and IL-6 significantly decreased in the treatment group and the control group,and NO increased in the treatment group;And IL-6 level was significantly lower and NO level was higher in the treatment group than those in the control group(4.48±1.22 ng/L vs 5.13±1.85 ng/L,117.4±22.3 μmol/L vs 92.4±17.1 μmol/L;both P<0.05);There was a positive correlation between IL-6 and IL-10,and a negative correlation between NO and IL-10(r=0.142,r=-0.152;both P<0.05).Conclusion:Liandou Qingmai Recipe can decline IL-6,IL-10 and ET-1 levels,and raise NO level in patients with coronary heart disease on the basis of standard treatment,so as to inhibit endothelial inflammatory response,improve vascular endothelial function,with stronger anti-AS action;And vascular endothelial lesion is related with inflammatory response.     

Role of Coagulation Factor Ⅶ in Pathogenesis of Ischemic Heart Disease

To study the variation and significance of plasma coagulation factor Ⅶ (FⅦ) in differ ent kinds of ischemia heart disease (IHD) and examine its relation with plasma lipid and gene polymorphism. FⅦa was determined with one stage clotting assay by using a recombinant soluble tissue factor (rsTF). FⅦc was measured with one stage clotting assay. FⅦag was quantified with an enzyme-linked immunosorbent assay (ELISA). Polymorphism was analyzed with PCR-urea-polyacrylamide gel electrophoresis. Our results showed that FⅦa in stable angina (SA),unstable angina (UA), obsolete and acute myocardial infraction (OMI, AMI) patients was higher than those of normal group with the differences being significant within any two groups. FⅦag in UA, OMI and AMI was higher than those in SA and normal groups. There were positive correlations between FⅦa and serum triglycerides, FⅦa and FⅦc, FⅦc and FⅦag. FⅦ-323 0/10 bp polymorphism analysis was performed in 60 patients and 0/10 bp polymorphism was found in 5 cases. FⅦc and FⅦag were much lower in cases of 0/10 bp groups than those in cases of 0/0 bp groups. It is concluded that there was activation of extrinsic coagulation pathway in every kind of IHD to different extent. FⅦa was the risk factor in the development of IHD, and more sensitive in reflecting the severity of cardiovacutar disease than FⅦc or FⅦag. FⅦa was higher in OMI, which may be one of the risk factors of re-infraction. Serum triglyceride may indirectly lead to the development of IHD by increasing the level of FⅦa. FⅦ-323 0/10 bp polymorphism was present in Chinese patients with IHD and it was correlated with the level of FⅦc, FⅦag in plasma. 10 bp allelomorphic gene was a protective factor against thrombogenesis.     

缺血性心脏病与β-纤维蛋白原基因bcl-I多态性和血浆纤维蛋白原水平的关系

目的:探讨β纤维蛋白原基因bcl-I多态性与血浆纤维蛋白原水平及缺血性心脏病发病机制的关系。方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对57例心肌梗塞组和50例健康对照组进行β-纤维蛋白原基因bcl-I多态性分析;采用组织凝血活酶法测定血浆纤维蛋白原水平。结果:酶切后可见在2 500 bp和1 100 bp+1 400 bp碱基对位置分别发现2个等位基因B1和B2。携带B1等位基因心肌梗塞患者血浆纤维蛋白原水平(3.28±0.94)g.L-1明显高于健康对照组(2.74±0.54)g.L-1(P<0.05),携带B2等位基因心肌梗塞患者血浆纤维蛋白原水平(3.97±1.02)g.L-1明显高于健康对照组(3.25±0.61)g.L-1(P<0.05);B2等位基因频率明显增高(P<0.05);B2基因携带者血浆纤维蛋白原水平明显高于同组内B1基因携带者血浆纤维蛋白原水平(P<0.05)。结论:血浆纤维蛋白原水平升高与心肌梗塞有关联;β-纤维蛋白原基因bcl-I多态性与血浆纤维蛋白原水平及心肌梗塞有关联,β-纤维蛋白原基因bcl-I多态性可能是缺血性心脏病危险因素及遗传易感标志之一。     

干细胞移植治疗缺血性心脏病

缺血性心脏病目前尚缺乏有效的治疗手段,大量的动物实验和小规模临床研究显示,干细胞移植可能有助于改善心脏功能,但在安全性方面仍有争议.     

老年冠心病患者肿瘤坏死因子-β基因多态性的研究

目的　对老年冠心病 (CHD)患者肿瘤坏死因子 - β (TNF - β)基因多态性进行分析 ,探讨其在冠心病发病中的作用。方法　用PCR法测定 4 0例CHD患者及 3 0例对照者的TNF - β等位基因频率并进行比较。 结果　TNF - β等位基因频率分布 ,两组之间比较差异无显著性意义 ( P >0 0 5 )。结论　TNF - β基因多态性在CHD发病机理中可能不起重要作用     

心包脂肪在缺血性心脏病中作用及机制的研究进展

缺血性心脏病是世界范围内高发病率和高死亡率的疾病之一,持续的缺血最终导致心肌梗死。心包脂肪作为邻近心脏的组织结构,可能通过内分泌或旁分泌作用参与缺血性心脏病的发生和发展。缺血性心脏病患者心外膜脂肪组织的体积、厚度以及密度均发生明显改变,且与疾病的不良风险因素和严重程度密切相关。心包脂肪中的免疫细胞及其分泌产物分别通过不同的方式参与缺血性心脏病。本文主要对心包脂肪在缺血性心脏病患者诊断和预后评价中的作用以及心包脂肪参与缺血性心脏病发生、发展的机制进行综述。     

缺血性心脏病与β-纤维蛋白原基因bcl-I多态性和血浆纤维蛋白原水平的关系

目的：探讨β纤维蛋白原基因bcl-I多态性与血浆纤维蛋白原水平及缺血性心脏病发病机制的关系。方法：应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对57例心肌梗塞组和50例健康对照组进行β-纤维蛋白原基因bcl-I多态性分析；采用组织凝血活酶法测定血浆纤维蛋白原水平。结果：酶切后可见在2 500 bp和1 100 bp+1 400 bp碱基对位置分别发现2个等位基因B1和B2。携带B1等位基因心肌梗塞患者血浆纤维蛋白原水平（3.28±0.94）g·L^-1明显高于健康对照组（2.74±0.54）g·L^-1（P<0.05），携带B2等位基因心肌梗塞患者血浆纤维蛋白原水平（3.97±1.02）g·L^-1明显高于健康对照组（3.25±0.61）g·L^-1（P<0.05）；B2等位基因频率明显增高（P<0.05）；B2基因携带者血浆纤维蛋白原水平明显高于同组内B1基因携带者血浆纤维蛋白原水平（P<0.05）。结论：血浆纤维蛋白原水平升高与心肌梗塞有关联；β-纤维蛋白原基因bcl-I多态性与血浆纤维蛋白原水平及心肌梗塞有关联，β-纤维蛋白原基因bcl-I多态性可能是缺血性心脏病危险因素及遗传易感标志之一。     

KDR基因rs2305948、rs2239702多态性与缺血性中风、冠心病痰瘀证凝血功能的关联性研究

目的探讨激酶插入结构域受体(KDR)基因多态性与缺血性中风(IS)痰瘀证、冠心病(CAD)痰瘀证及凝血功能的关联性。方法共纳入缺血性中风痰瘀证患者550例、冠心病痰瘀证患者550例、正常对照人群550例,分为3组。采用MassARRAY SNP基因分型实验技术进行基因分型检测,使用实时荧光定量PCR(RT-PCR)方法检测外周血KDR基因mRNA表达水平,通过凝固法检测血浆中凝血功能指标。采用PLINK软件进行遗传关联分析,采用拟和优度χ~2检验进行哈温平衡(HWE)分析。基因型频率分布的组间比较,采用χ~2检验。应用多因素非条件Logistic回归模型对各遗传模型(加性模型、显性模型、隐性模型)与疾病的关联进行分析,采用线性回归分析各位点多态性与凝血标志物水平的关联。结果 IS痰瘀证患者的KDR基因mRNA表达水平显著低于对照组(P<0.001),而CAD痰瘀证患者的KDR基因mRNA表达水平显著高于对照组(P=0.007)。KDR基因rs2305948、rs2239702多态性与IS痰瘀证、CAD痰瘀证发生风险的关联差异无统计学意义(均为P>0.05)。KDR基因rs2305948多态性与IS痰瘀证患者的D二聚体(D-D)、凝血酶原时间活动度(PTA)的相关性具有统计学意义(P<0.05),KDR基因rs2239702多态性与IS痰瘀证患者的活化部分凝血酶原时间(APTT)显著相关(P<0.05)。KDR基因rs2305948多态性与CAD痰瘀证患者APTT水平的相关性具有统计学意义(P<0.05),rs2239702多态性与CAD痰瘀证患者凝血酶时间(TT)水平显著相关(P<0.05)。结论 KDR基因表达水平可能影响IS痰瘀证、CAD痰瘀证的发生,且KDR基因rs2305948、rs2239702遗传多态性可能影响IS痰瘀证、CAD痰瘀证的凝血功能。     

重组凝血因子Ⅶ表达和合成机制的研究进展

血友病是由于人体内缺乏相应凝血因子造成的凝血障碍,目前主要利用基因重组技术在不同细胞中表达重组凝血因子Ⅶ(Ⅶ因子)从而对血友患者出血进行治疗。为有效提高重组Ⅶ因子的产量及活性,已有研究者尝试利用不同真核表达系统合成重组Ⅶ因子,如BHK细胞、CHO细胞、昆虫细胞和鱼胚胎等。同时,不同外源功能性基因对重组Ⅶ因子活性和翻译后修饰对表达产量的影响也通过不同方法进行探究。本文从Ⅶ因子在凝血途径中的作用、重组表达重组Ⅶ因子和翻译后修饰对合成的影响3个方面对重组Ⅶ因子表达和合成机制研究进行了综述。     

曲美他嗪治疗老年性缺血性心脏病心力衰竭的临床疗效

目的：观察曲美他嗪治疗老年性缺血性心脏病心力衰竭的疗效。方法：对我院107例缺血性心脏病心力衰竭患者在常规治疗基础上应用曲美他嗪进行治疗。结果：经过治疗患者所有指标均有显著改善,与治疗前比较,差异有统计学意义（P〈0．05）,其中显效75例（70．1％）,有效28例（26．2％）,无效4例（3．7％）,总有效率为96．3％,无明显不良反应。结论：在常规治疗基础上,应用曲美他嗪治疗老年性缺血性心脏病心力衰竭疗效确切,心脏功能明显改善,临床耐受性好。     

补体因子H基因~(1277)T/C多态性与早发冠心病的相关性研究

目的:探讨补体因子H(CFH)基因1277T/C多态性与早发冠心病的相关性。方法:选择早发冠心病患者(男<55岁,女<60岁)125例、非冠心病对照组126例,收集研究对象临床资料,检测生化指标。采用聚合酶链反应-连接酶检测反应(PCR-LDR)分析CFH基因1277T/C多态性。结果:早发冠心病组吸烟、高血压病及糖尿病比例较对照组明显增多(P<0.05)。早发冠心病组与对照组CFH基因1277T/C中TT、TC、CC基因型频率分布差异无统计学意义(χ2=0.081,P>0.05)。结论:CFH基因1277T/C多态性可能与早发冠心病的易感性无关。