骨桥蛋白与血管损伤的研究进展

骨桥蛋白(osteopontin,OPN)是最早从骨基质分离出的一种磷酸化糖蛋白,富含唾液酸.因其介导骨组织细胞与骨基质的连接,参与骨基质矿化和重吸收过程而得名.OPN作为一种新型的细胞因子,骨桥蛋白参与多种生命现象,如骨代谢、肿瘤的生长转移、炎症与免疫等,近年来的研究表明OPN在大鼠血管损伤后新生内膜中的表达明显上调,而且它能促进血管平滑肌细胞、外膜细胞的迁移,被认为是血管损伤修复过程中重要的始动因素[1].本文对OPN的基本性征以及它在血管损伤中的作用予以综述.     

骨桥蛋白与血管再狭窄的关系

骨桥蛋白(osteopontin,OPN)是细胞外基质中一种重要的功能性蛋白,因其介导骨细胞与骨基质的连接、参与骨基质矿化和重吸收过程而得名,它在巨噬细胞、内皮细胞、血管平滑肌细胞(VSMC)及上皮细胞等中也有表达.作为一种新型的细胞因子,OPN参与多种生命现象,如骨代谢、肿瘤的生长转移、炎症与免疫等,尤其在组织的损伤与修复中受到广泛的关注.近年来的研究表明OPN在血管损伤后新生内膜中的表达明显上调,而且它能促进VSMC和外膜细胞的迁移,被认为是血管损伤修复过程中重要的始动因素[1].本文对OPN的基本特征以及它在血管再狭窄中的作用予以综述.     

骨桥蛋白与血管损伤的研究进展

骨桥蛋白(osteopontin,OPN)是最早从骨基质分离出的一种磷酸化糖蛋白,富含唾液酸。因其介导骨组织细胞与骨基质的连接,参与骨基质矿化和重吸收过程而得名。OPN作为一种新型的细胞因子,骨桥蛋白参与多种生命现象,如骨代谢、肿瘤的生长转移、炎症与免疫等,近年来的研究表明OPN在大鼠血管损伤后新生内膜中的表达明显上调,而且它能促进血管平滑肌细胞、外膜细胞的迁移,被认为是血管损伤修复过程中重要的始动因素[1]。本文对OPN的基本性征以及它在血管损伤中的作用予以综述。1OPN的分子生物学特征OPN分子量约66kD,富含天冬氨酸、丝氨酸和…     

骨桥蛋白的制备及功能研究

目的　探讨骨桥蛋白 (OPN)对血管平滑肌细胞是否具有促黏附及趋化作用。方法　从大鼠源性血管平滑肌细胞的细胞培养基中纯化OPN ,制备家兔抗OPN多克隆抗体。用酶联免疫吸附及迁移分析方法测定OPN对血管平滑肌细胞的影响。结果　骨桥蛋白能够促进血管平滑肌细胞发生黏附 ,且其促黏附功能呈浓度依赖性。黏附率在实验开始后 90～ 1 2 0min区段达到最高。与对照相比 ,OPN刺激血管平滑肌细胞的迁移距离明显增加。结果　骨桥蛋白是血管平滑肌细胞的黏附、迁移活化因子     

骨桥蛋白与心血管系统疾病

骨桥蛋白是一种内分泌性非胶原型糖基化磷蛋白,可由破骨细胞、血管平滑肌细胞、血管内皮细胞、巨噬细胞等多种细胞表达,其表达受多种因素的影响.正常动脉壁及主动脉瓣膜中骨桥蛋白几乎不表达,动脉粥样硬化、血管及主动脉瓣膜钙化和新生内膜形成时,其水平显著升高.     

骨桥蛋白与血管再狭窄

骨桥蛋白（OPN）是细胞外基质中一种重要的功能性蛋白，研究表明OPN在血管损伤后新生内膜中的表达明显上调，而且它能促进血管平滑肌细胞、外膜细胞的迁移，被认为是血管损伤修复过程中重要的始动因素，参与血管再狭窄形成，在动脉粥样硬化、血管再狭窄及动脉钙化发生过程中起着重要作用，应用骨桥蛋白及其抑制剂可能有助于血管再狭窄的防治。     

骨桥蛋白与心血管系统疾病

骨桥蛋白是一种内分泌性非胶原型糖基化磷蛋白，可由破骨细胞、血管平滑肌细胞、血管内皮细胞、巨噬细胞等多种细胞表达，其表达受多种因素的影响。正常动脉壁及主动脉瓣膜中骨桥蛋白几乎不表达，动脉粥样硬化、血管及主动脉瓣膜钙化和新生内膜形成时，其水平显著升高。     

骨桥蛋白在类风湿关节炎发病机制中的作用

骨桥蛋白（osteopontin，OPN），是一种早在1979年从骨细胞外基质分离出的磷酸化糖蛋白，又称早T淋巴细胞活化因子1（Eta—1），后来逐渐发现OPN表达于各种细胞，包括破骨细胞、巨噬细胞、活化的T细胞、上皮细胞、内皮细胞、平滑肌细胞，且存在于各种组织中包括骨、肾脏、胎盘、平滑肌、分泌性上皮。OPN参与多种生物学过程。包括细胞的趋化、聚集、黏附、增殖和迁移、免疫调节、信号转导、骨的再吸收、伤口愈合、组织修复及动脉粥样硬化、肾脏疾病和肿瘤的发生。最近发现OPN分别通过整合素αv、β3和CD44受体增加巨噬细胞表达白细胞介索（IL）-12，抑制IL-10表达，在自身免疫性疾病的发展中起了重要作用。本文就其在类风湿关节炎（rheumatoid arthritis，RA）中可能的作用机制作一综述。     

骨桥蛋白及其在动脉粥样硬化中的作用

骨桥蛋白（osteopontin，OPN）是细胞外基质中一种重要的糖蛋白，骨组织中大量存在，其他组织中也有少量的表达，但正常血管壁中几乎不表达。1986年以前曾被称为早期T淋巴细胞活性1（early T lymphocyte activation，Eta-1）、分泌性磷蛋白1（secrected phosphoprotein 1，SSP-1）、骨唾液酸蛋白1（bone sialoprotein 1，BSP-1），自从被命名为OPN以来，已相继克隆了大鼠、小鼠、猪、牛、鸡和兔的OPN cDNA。近来研究发现在血管壁动脉粥样硬化处的内皮细胞和平滑肌细胞内OPN mRNA呈高表达状态。它在动脉粥样硬化中的作用已经引起人们的关注。本文就OPN及其在动脉粥样硬化中的作用作一综述。     

内皮祖细胞对血管紧张素Ⅱ诱导的血管平滑肌细胞表型转化的影响

目的 观察内皮祖细胞对血管紧张素Ⅱ诱导的血管平滑肌细胞表型转化的影响.方法 采用6%羟乙基淀粉沉降法和密度梯度离心法分离人脐血单个核细胞,EGM-2细胞培养基进行培养,诱导单个核细胞贴壁向内皮祖细胞分化.采用荧光显微镜双染色、流式细胞术鉴定内皮祖细胞,间接免疫荧光检测血管平滑肌细胞标志物平滑肌α-肌动蛋白、钙调节蛋白的表达,采用逆转录聚合酶链反应和免疫印迹检测早期内皮祖细胞条件培养液、晚期内皮祖细胞条件培养液以及人脐静脉内皮细胞条件培养液对血管紧张素Ⅱ诱导的血管平滑肌细胞收缩表型标志基因平滑肌α-肌动蛋白以及合成表型标志基因骨桥蛋白表达变化的影响.结果 与对照组比较,血管紧张素Ⅱ(10-6mmol/L)诱导血管平滑肌细胞增殖48 h后,平滑肌α-肌动蛋白mRNA和蛋白表达明显减少,而骨桥蛋白mRNA和蛋白表达明显增加,提示血管平滑肌细胞从收缩表型向合成表型转化;与血管紧张素Ⅱ组比较,早期内皮祖细胞条件培养液、晚期内皮祖细胞条件培养液以及人脐静脉内皮细胞条件培养液处理后均不同程度抑制血管紧张素Ⅱ诱导的平滑肌α-肌动蛋白表达减少和骨桥蛋白表达增加,其中以早期内皮祖细胞条件培养液的抑制效果最明显.结论 内皮祖细胞能够抑制血管紧张素Ⅱ诱导的血管平滑肌细胞从收缩表型向合成表型转化.     

Effects of Simvastatin on Ion Channel Currents in Ventricular Myocytes from Acute Infarcted Heart of Normocholesterolemic Rabbits

Objectives To investigate the effects of simvastatin on membrane ionic currents in left ventricular myocytes of rabbit heart suffering from acute myocardial infarction （ AMI）, so as to explore the ionic mechanism of statin treatment for antiarrhythmia. Methods Forty-five New Zealand rabbits were randomly divided into three groups： AMI group, simvastatin intervention group （ Statin group） and sham-operated control group （CON）. Rabbits were infarcted by ligation of the left anterior descending coronary artery after administration of oral simvastatin 5 mg · kg^-1·d^-1 （Statin group） or placebo （AMI group） for 3 days. Single ventricular myocytes were isolated enzymatically from the epicardial zone of the infracted region 72 h later. Whole cell patch clamp technique was used to record membrane ionic currents, including sodium current （INa）, L-type calcium current （Ica-L） and transient outward potassium current （Ito）. Results （1） There was not significant difference in serum cholesterol concentration among three groups. （2） The peak INa current density （at -30 mV） was significantly decreased in AMI group （ -25.26±5.28, n = 13 ）, comparing with CON （ - 42. 78± 5.48, n = 16）, P 〈 0. 05, while it was significantly increased in Statin group （ - 39.83 ±5.65 pA/pF, n = 12） comparing with AMI group, P 〈0. 01 ; The peak Ica-L current density （ at 0 mV） was significantly decreased in AMI group （ -3. 43 ±0. 92 pA/pF, n = 13） comparing with CON （ -4. 56 ±1.01 pA/pF, n = 15）, P 〈0. 05, while it was significantly increased in Statin group （ -4. 18±0. 96 pA/pF, n = 12） comparing with AMI group, P 〈0. 05; The Ito current density （ at ＋ 60 mV） was significantly decreased in AMI group （ 11.41 ± 1.94 pA/pF, n = 13 ） comparing with CON （17.41 ±3.13 pA/pF, n = 15）, P 〈0. 01, while it was significantly increased in Statin group （16. 11 ± 2. 43 pA/pF, n = 14） comparing with AMI group, P 〈 0. 01. Conclusions AMI induces signific     

Risk Factors and Relation Between Clinical,Biochemical Marker and Stenosis Extent of Coronary Artery in Young Adults With Acute Myocardial Infarction

Objectives To analyze risk factors and the relation between clinical, biochemical marker and the stenosis extent of coronary artery in patients below the age of 45 years with acute myocardial infarction （AMI）. Methods A retrospective investigation was performed on 92 patients below the age of 45 with AMI at the First Affiliated Hospital of Medical School of Xi′an Jiaotong University in 2003-2007. The etiology, morbidity, risk factors, clinical features and results of coronary angiography were studied. Various clinical and biochemical markers were assessed to find out what were associated with the stenosis extent of coronary artery. Meanwhile, the differences between one-vessel disease （group A） and two-vessel or multi-vessel disease （group B） patients with AMI were comparatively analyzed. Results Risk factors analysis revealed that a history of cigarette smoking, metabolic disorders and abusive drinking were mainly found in young AMI patients below the age of 45 years, and metabolic disorder mainly consists of decreased high-density lipoprotein cholesterol （HDL-C） and hypertriglyceridemia. AMI in patients below the age of 45 years account for 10.3% of all AMI. Angiographically, the incidence of one-vessel affected was most frequent in the young adults （73.75 %）. The most committed vessel was LAD （80.00 %）. A higher incidence with history of hypertension and diabetes or impaired glucose tolerance was found in group B, but a history of preceding angina 1 month earlier was more frequently found in group A. Improved Genisi scores of coronary angiography was lower in group A than in group B（7.49±3.63 vs 15.08±6.08）. Correlation analysis showed that log（LDL-C/HDL-C）（r= 0.238, P= 0.037）, TC/HDL-C （r= 0.232, P= 0.046） were directly correlated with angiographic scores, and HDL-C（r= -0.202, P= 0.042）was inversely correlated. Multielement gradual linear regression analysis showed log（LDL-C/HDL-C）, TC/HDL-C were associated with the extent of stenosis of coronary artery. Fur     

一例急性心肌梗死合并血小板减少症患者的PCI策略及抗血小板治疗

1 病例资料 患者男性,58岁,因劳累再发胸痛7d入院,高脂血症及2型糖尿病史3年,23年前服用阿司匹林半月导致血小板减少,停用后血小板数目在50×109／L左右波动,未行诊治。3年前患急性下壁心肌梗死,回旋支植入2枚支架,右侧冠脉植入2枚支架（支架类型不详）,服用阿司匹林100mg／d、氢氯吡格雷75mg／d,共20d,血小板降至20×109／L,咨询医生停药。无胸痛,检测血小板波动于40×109／L～50×109／L。     

Increasing Uric Acid Concentration in Elderly With Ischemic Cardiomyopathy

Objectives Plasma uric acid （UA） concentration was suspected to elevate in elderly with ischemic cardiomyopathy （ICM）. Methods We analyzed the data of 235 elderly aged 60 years and older with coronary heart disease： silent myocardial ischemia or angina pectoris confirmed by angiography. Among these patients, 154 had ICM defined as left ventricular end-diastolic diameter （LVDd） male 〉 55 mm, female 〉 50 mm （mean. 63.51 ± 7.70 mm） measured by echocardiography. Difference in UA was analyzed between patients with and without ICM. Results There was significant increase of UA in ICM compared with non-ICM （432.82 ± 143.05 umol/L vs 361. 06 ± 137.35 umol/L, P 〈 0. 05 ） ; and UA was positively related to LVDd （ r = 0. 25, P 〈 0. 05 ）. Conclusions There was significant increase of UA in elderly with ICM due to longterm silent myocardial ischemia and angina pectoris. Moreover, UA was positively related to LVDd. （ S Chin J Cardiol 2009; 10（4） ： 212 -215）     

主动脉内球囊反搏治疗急性广泛前壁心肌梗死合并室间隔穿孔一例

室间隔穿孔是急性心肌梗死少见但却非常严重的机械并发症之一,常发生于急性心梗的早期1～2周,占急性心梗患者1％～3％[1].其预后差,死亡率极高.主动脉内球囊反搏（IABP）是目前国内应用较多的心脏辅助装置,能够提高疗效,提高生存率.本病例在药物治疗基础上,早期植入IABP,使患者心功能得到一定的改善,度过危险期,赢得了最佳的抢救时机。     

The Utility of B-type Natriuretic Peptide to Predict Prognosis of Acute Myocardial Infarction Patients Complicated With Cardiogenic Shock Treated With Intra-aortic Ballon Counterpulsation

Objectives To assess the prognostic value of B-type natriuretic peptide （BNP） in severe AMI patients treated with intra-aortic ballon counterpulsation（IABP）. Methods A total of 42 AMI patients with cardiogenic shock were retrospectively studied. BNP plasma level was recorded in the 24th hour and 4th day after myocardial infarction. The different mortality were compared among patients with different BNP levels. Results With aggressive treatment, 20 patients survived short term hospitalization. Plasma concentration of BNP in dying patients is much higher than in survivals（1369 ± 353 vs 651 ± 302 pg/ml. P〈 0.01）.Patients with BNP higher than 1474 pg/mL had a mortality of 92.9 %. Conclusions Elevated BNP level in AMI patients with cardiogenic shock treated with IABP is highly associated with poor prognosis.     

Effects of Bradykinin B2 Receptor Blockade on Infarct Size and Hemodynamics after Myocardial Infarction in Enalapril-treated Rats

Objectives To study the effects of bradykinin （BK） B2 receptor blockade on infarct size and hemodynamics after myocardial infarction （MI） in rats with angiotensin-converting enzyme （ACE） inhibition therapy. Methods MI was produced by ligating the left coronary artery. The effects of enalapril （ 500 μg/kg·day）, enalapril （ 500 μg/kg · day） with BK B2 receptor antagonist Hoe-140 （500 μg/kg · day）, angiotensin Ⅱ （Ang Ⅱ） type 1 （AT1） receptor antagonist losartan （3 mg/kg · day） on infarct size, left ventricular systolic pressure （ LVSP）, cardiac output index （CI） and stroke volume index （SVI） were observed in rats after MI. Treatments were started on the 2nd day after MI and continued for another 6 weeks. Results Enalapril reduced infarct size and improved CI and SVI compared with the untreated MI group （ P 〈 0. 05 ）, and these effects of enalapril were significantly blunted by concomitant treatment with Hoe-140 （P 〈 0. 05）. Losartan was less effective than enalapril. LVSP were unchanged in the three treatment groups. Conclusions BK can reduce infract size and improve hemodynamics in rats following MI. The cardioprotective effects of ACEI partly result from the action of BK exerted through the B2 receptor.     

Changes of Clinical Features of Aortic Dissection Over Ten Years

Objectives To investigate changes of clinical features of aortic dissection （AD） in recent years in Guangzhou. Methods Retrospective analysis of consecutive patients with AD over 10 years in Guangdong Cardiovascular Institute. Demographic features and outcomes in cases presenting with acute dissection were compared between patients admitted in two five-year periods. Results 339 patients with AD were admitted during the recent ten years. Their mean age was 55.7 ± 11.2 years. Male verse female ratio was 4.8. Surgery was performed in 37 % of cases with acute type A and 7 % with acute type B dissection. Apart from a fast increase in number of patients admitted each year, there were no significant changes in demographic characteristics over these ten years. Survival of patients with acute dissection showed a trend of improvement, though it had not been statistically confirmed. Conclusions The number of admitted patients with AD has been fast increasing in Guangzhou. These patients were about ten years younger than previously reported by foreign researchers. Surgery was much less frequently performed than it was in western countries. Survival in acute cases showed a trend of improvement. These data support urgent improvement in prevention and management of aortic dissection.     

两次食管静脉曲张硬化治疗后均发生化脓性胆囊炎1例报告

<正>1病历资料男性,患者,41岁,2007年因乙型肝炎肝硬化、食管胃底静脉曲张破裂出血于外院行内镜下食管静脉曲张硬化治疗术,术后患者出现化脓性胆囊炎,经保守治疗后病情缓解。该患者2013年8月因再次"呕血、黑便1 d"入住威海市立医院,本次住院前曾行B超检查:肝硬化,门静脉高压,脾静脉、肠系膜上静脉、下腔静脉肝后段栓塞,胆囊多发结石,胆囊壁厚。住院后随即行相关化验检查,血常规:白细胞(WBC)4.7×109/L,中性粒     

非酒精性脂肪性肝病的饮食治疗

随着高脂肪高热量膳食的盛行、生活节奏的加快和多坐少动生活方式的流行,肥胖、糖尿病及其相关非酒精性脂肪性肝病（Nonalcoholic fatty liver diseases,NAFLD）已成为愈来愈重要的慢性非传染性流行病。在重度肥胖症患者中,NAFLD的发病率高达90%,其中20%～30%为非酒精性脂肪性肝炎（NASH）,高达8%患者已发展至肝硬化阶段。