Muscle tissue oxygenation, pressure, electrical, and mechanical responses during dynamic and static voluntary contractions

Dynamic muscle contractions have been shown to cause greater energy turnover and fatigue than static contractions performed at a corresponding force level. Therefore, we hypothesized that: (1) electro- (EMG) and mechanomyography (MMG), intramuscular pressure (IMP), and reduction in muscle oxygen tension (rTO₂) would be larger during dynamic (DYN) than intermittent static (IST) low force contractions; and that (2) oxygen tension would remain lower in the resting periods subsequent to DYN as compared to those following IST. Eight subjects performed elbow flexions with identical time-tension products: (1) DYN as a 20° elbow movement of 2 s concentric and 2 s eccentric followed by a 4 s rest; and (2) IST with a 4 s contraction followed by a 4 s rest. Each session was performed for 1 min at 10 and 20% of the maximal voluntary contraction (MVC). The force, bipolar surface EMG, MMG, IMP, rTO₂ were measured simultaneously from the biceps brachii, and the data presented as the mean values together with the standard error of the means. Comparison of the corresponding time periods showed the EMG_rms and MMG_rms values to be larger during DYN than IST (concentric phase: DYN vs IST were 14.2 vs 9.4, and 22.0 vs 15.9%_max−EMG_rms; eccentric phase: in DYN, the MMG was ~1.5 and ~2.0-fold IST at 10 and 20%MVC, respectively). In contrast, the IMP of the concentric phase in DYN was lower than in IST (2.3 vs 29.5 and 10.9 vs 42.0 mmHg at 10 and 20%MVC, respectively), and a similar picture was seen for the eccentric phase. However, no differences were seen in rTO₂ in either the contraction or the rest periods. In a prolonged rest period (8 s) after the sessions, DYN but not IST showed rTO₂ above baseline level. In conclusion, rTO₂ in DYN and IST were similar in spite of major differences in the MMG and EMG responses of the muscle during contraction periods. This may relate to the surprisingly lower IMP in DYN than IST.     

Intramuscular pressure and tissue oxygenation during low‐force static contraction do not underlie muscle fatigue

Aim: To test the hypothesis that time‐wise increase in intramuscular pressure (IMP) and subsequent decrease in muscle tissue oxygenation (TO₂) results in muscle fatigue development during a non‐exhaustive, low‐force contraction evidenced by changes in electromyogram (EMG) and particular mechanomyogram (MMG). Methods: Seven subjects performed static elbow flexion at 10% maximal voluntary contraction (MVC) for 10 min (10% MVC_{10 min}). Surface EMG, MMG, IMP and TO₂ measured by near‐infrared spectroscopy was recorded from m. biceps brachii during 10% MVC_{10 min} and during 5% MVC test contractions of 1 min duration performed before 10% MVC_{10 min}, 10 and 30 min post‐exercise. EMG and MMG were analysed for root mean square (rms) and mean power frequency (mpf). Results: During 10% MVC_{10 min} MMGrms increased from initial level of 0.04 ± 0.01 to 0.11 ± 0.07 m s⁻² in the last minute and MMGmpf and EMGmpf decreased from 34.9 ± 8.2 to 21.3 ± 3.8 Hz and from 71.7 ± 10.9 to 61.7 ± 10.0 Hz respectively. Similar changes were present in 5% MVC test contractions 30 min post‐exercise. Initially, TO₂ decreased by 6.9 ± 6.5% of resting level but returned to rest within 1 min. IMP remained constant during the contraction after an initial fourfold increase from resting level of 12.2 ± 10.4 mmHg. Conclusions: IMP was anticipated to increase with time of contraction due to e.g. increased muscle water content; but this was not confirmed. Consequently, muscle blood flow was unlikely to be impeded with contraction time, which may account for the maintenance of TO₂. Thus, decreased TO₂ did not underlie either acute or long‐term muscle fatigue development evidenced by changes in EMG and particular MMG variables.     

Intramuscular pressure and tissue oxygenation during low-force static contraction do not underlie muscle fatigue

AIM: To test the hypothesis that time-wise increase in intramuscular pressure (IMP) and subsequent decrease in muscle tissue oxygenation (TO(2)) results in muscle fatigue development during a non-exhaustive, low-force contraction evidenced by changes in electromyogram (EMG) and particular mechanomyogram (MMG). METHODS: Seven subjects performed static elbow flexion at 10% maximal voluntary contraction (MVC) for 10 min (10% MVC(10 min)). Surface EMG, MMG, IMP and TO(2) measured by near-infrared spectroscopy was recorded from m. biceps brachii during 10% MVC(10 min) and during 5% MVC test contractions of 1 min duration performed before 10% MVC(10 min), 10 and 30 min post-exercise. EMG and MMG were analysed for root mean square (rms) and mean power frequency (mpf). RESULTS: During 10% MVC(10 min) MMGrms increased from initial level of 0.04 +/- 0.01 to 0.11 +/- 0.07 m s(-2) in the last minute and MMGmpf and EMGmpf decreased from 34.9 +/- 8.2 to 21.3 +/- 3.8 Hz and from 71.7 +/- 10.9 to 61.7 +/- 10.0 Hz respectively. Similar changes were present in 5% MVC test contractions 30 min post-exercise. Initially, TO(2) decreased by 6.9 +/- 6.5% of resting level but returned to rest within 1 min. IMP remained constant during the contraction after an initial fourfold increase from resting level of 12.2 +/- 10.4 mmHg. CONCLUSIONS: IMP was anticipated to increase with time of contraction due to e.g. increased muscle water content; but this was not confirmed. Consequently, muscle blood flow was unlikely to be impeded with contraction time, which may account for the maintenance of TO(2). Thus, decreased TO(2) did not underlie either acute or long-term muscle fatigue development evidenced by changes in EMG and particular MMG variables.     

Differential responses in intramuscular pressure and EMG fatigue indicators during low- vs. high-level isometric contractions to fatigue

This study investigated changes in intramuscular pressure (IMP) and surface electromyographic (EMG) parameters (mean frequency of the power spectrum, f_mean; and signal amplitude denoted as root mean square, RMS) during contractions to fatigue at 25 and 70% of maximal voluntary contraction (MVC). Parameters were recorded simultaneously from the vastus lateralis muscle during knee extension. A significant decrease in f_mean occurred with time at both contraction levels; however, the rate of decline (slope) was greater at 70% MVC. RMS increased throughout the contractions at both levels, with the relative increase being significantly greater for 25% MVC. IMP increased during 25% MVC but did not change during the 70% MVC contraction. IMP at rest was significantly higher post-contractions than it was pre-contractions at 25% MVC (21.1 vs. 8.0 mmHg, P < 0.01) and 70% MVC (13.7 vs. 8.6 mmHg, P < 0.01). Consequently, post-contraction IMP was higher at 25% MVC than at 70% MVC (P < 0.01). IMP changes throughout the fatiguing contractions correlated negatively with f_mean and positively with RMS at both MVC levels; however, these correlations were better at 25% MVC. The extent of intramuscular water accumulation is discussed as a major cause of the difference in IMP changes between 25% and 70% MVC. Significant differences in the rate of change for all parameters between high vs. low contraction levels may suggest a common mechanism governing changes in IMP and EMG fatigue indicators.     

Development of muscle fatigue as assessed by electromyography and mechanomyography during continuous and intermittent low-force contractions: effects of the feedback mode

The goal of the present study was to investigate the significance of low-force continuous or intermittent static contraction and feedback mode (visual or proprioceptive) on the development of muscle fatigue as assessed by electromyography (EMG) and mechanomyography (MMG). Visual (force control) and proprioceptive (displacement control) feedback was investigated during intermittent (6 s contraction, 4 s rest) and continuous static contractions at 10% and 30% of the maximum voluntary contraction (MVC). Mean force, force fluctuation, rating of perceived exertion and root mean square (RMS) and mean power frequency (MPF) of the EMG and MMG signals were analysed. The general pattern for MMG RMS and EMG RMS values and the rating of perceived exertion was an increase with contraction time, while the EMG MPF values decreased (P<0.05). The increase in RMS values was generally more pronounced for the MMG compared with the EMG, while the decrease in MPF values was more consistent for the EMG compared with the MMG signal. During the intermittent contractions, the main effect was on MPF for both EMG and MMG. Lower force fluctuation and larger rating of perceived exertion (P<0.05), greater slopes of EMG and MMG RMS and MPF values versus time were observed with proprioceptive feedback compared with visual feedback. The findings suggest that (1) the EMG and MMG signals give complementary information about localised muscle fatigue at low-level contraction: they responded differently in terms of changes in the time and frequency domain during continuous contraction, while they responded in concert in the frequency domain during intermittent contractions, and (2) the different centrally mediated motor control strategies used during fatiguing contraction may be dependent upon the feedback modality. Electronic Publication     

EMG and MMG of synergists and antagonists during relaxation at three joint angles

The aim of the study was to estimate the influence of force changes during relaxation from maximal voluntary contraction (MVC) of elbow flexors on electrical (EMG) and mechanical (MMG) activity of synergists and anatgonists at different joint angles. Repeated studies were conducted on 22 young female students to estimate the EMG and MMG activity of the biceps brachii (BB), brachioradialis (BR), and triceps brachii (TB) muscles during relaxation from MVC at an optimal angle (the angle at which a subject achieved MVC=Lo), as well as at angles that were smaller (Ls=Lo–30°) and bigger (Ll=Lo+30°). Four testing sessions consisted of 2-s or 3-s MVC at each angle with simultaneous recording of EMG and MMG signals from BB, BR, and TB muscles. The EMG/MMG probes were used to record EMG and MMG signals. The results showed that the slow relaxation rate was related to a percentage decrease of the EMG amplitude (as measured by root mean square; EMG RMS) of the synergists, while the fast relaxation was related to the EMG RMS of the antagonist, independent of the joint angle. The MMG amplitude (MMG RMS) increased gradually during slow and fast relaxation (compared to the MMG RMS at MVC), indicating a bigger muscle oscillation during relaxation; the changes were related to the muscle tested and joint angle. It was found that, during the slow relaxation, the MMG RMS reflects the force amplitude changes in the BB muscle and the velocity of force changes in the BB and TB muscle (but not in the BR). During the fast relaxation, the MMG amplitude reflects a change in muscle force in the TB and BB muscles (but not in the BR), and the velocity of force changes in the synergists (not in the antagonist). The different contribution of the force and velocity of force changes during slow and fast relaxation to the MMG signal in the three muscle tested can be related to the different deactivation time of each muscle. In conclusion, the present results indicate that MMG recordings might be useful to measure the fast relaxation of individual muscle during voluntary contraction, but this needs be tested on isolated muscle.     

Mechanomyogram and force relationship during voluntary isometric ramp contractions of the biceps brachii muscle

The aim of the present study was to examine the non-stationary mechanomyogram (MMG) during voluntary isometric ramp contractions of the biceps brachii muscles using the short-time Fourier transform, and to obtain more detailed information on the motor unit (MU) activation strategy underlying in the continuous MMG/force relationship. The subjects were asked to exert ramp contractions from 5% to 80% of the maximal voluntary contraction (MVC) at a constant rate of 10% MVC/s. The root mean squared (RMS) amplitude of the MMG began to increase slowly at low levels of force, then there was a slight reduction between 12% and 20% MVC. After that, a progressive increase was followed by a decrease beyond 60% MVC. As to the mean power frequency (MPF), a relatively rapid increase up to 30% MVC was followed by a period of slow increment between 30% and 50% MVC. Then temporary reduction at around 50% MVC and a further rapid increase above 60% MVC was observed. The interaction between amplitude and MPF of the MMG in relation to the MU activation strategy is discussed for five force regions defined on the basis of their inflection points in the RMS-amplitude/force and MPF/force relationships. It was found that the MMG during ramp contractions enables deeper insights into the MU activation strategy than those determined during traditional separate contractions. In addition, this contraction protocol is useful not only to ensure higher force resolution in the MMG/force relationship, but also to markedly shorten the time taken for data acquisition and to reduce the risk of fatigue. Accepted: 31 August 2000     

Mechanomyogram from the different heads of the quadriceps muscle during incremental knee extension

To investigate the time- and frequency-domain responses of mechanomyograms (MMGs) during the progressive fatigue induced by intermittent incremental contractions, a surface MMG was obtained from the three muscle heads of the quadriceps muscle in seven subjects while they performed isometric knee extensions lasting 7.6 min. Isometric intermittent incremental contractions started at 1% of the maximal voluntary contraction (MVC) for 3 s, with a 3-s relaxation period in between each contraction, and the contraction level was increased by 1% of MVC for every contraction (by 10% of MVC per min) up to exhaustion. Separate contractions with sufficient rest periods were also conducted to serve for the MMG characteristics without fatigue. The integrated MMG (iMMG) was linearly related to force in all of the muscles when fatigue was not involved. With regard to the incremental contractions, the relationship exhibited an ascending-descending shape, but the behavior was not the same for the individual muscle heads, especially for the rectus femoris muscle. A steep increase in the median frequency of MMG from around 60% of MVC corresponded to a decrease in iMMG. These results suggest that analysis of MMG in the time- and frequency-domain during an incremental protocol is a useful way of characterizing the motor unit recruitment strategy and fatigue properties of individual muscles.     

The surface mechanomyogram as a tool to describe the influence of fatigue on biceps brachii motor unit activation strategy. Historical basis and novel evidence

The surface mechanomyogram (MMG) (detectable at the muscle surface as MMG by accelerometers, piezoelectric contact sensors or other transducers) is the summation of the activity of single motor units (MUs). Each MU contribution is related to the pressure waves generated by the active muscle fibres. The first part of this article will review briefly the results obtained by our group studying the possible role of motor unit recruitment and firing rate in determining the characteristics of the MMG during stimulated and voluntary contractions. The second part of this article will study the MMG and EMG during a short isometric force ramp from 0 to 90% of the maximal voluntary contraction (MVC) in fresh and fatigued biceps brachii. The aim is to verify whether changes in motor unit activation strategy in voluntarily fatigued muscle could be specifically reflected in the time and frequency domain parameters of the MMG. MMG-RMS vs. %MVC: at fatigue the MMG-RMS did not present the well known increment, when effort level increases, followed by a clear decrement at near-maximal contraction levels. MMG-MF vs. %MVC: compared to fresh muscle the fatigued biceps brachii showed an MF trend significantly shifted towards lower values and the steeper MF increment, from 65 to 85% MVC, was not present. The alteration in the MMG and EMG parameters vs. %MVC relationships at fatigue seems to be related to the impossibility of recruiting fast, but more fatigable MUs, and to the lowering of the global MUs firing during the short isometric force ramp investigated.     

Blood pressure, heart rate and EMG in low level static contractions

The purposes of the present investigation were: to evaluate a possible use of changes in the mean spectral frequency (MSF) of the EMG power spectra as a measure of reflex cardiovascular responses originating from the muscles during static exercise; and to study the relation between muscle fibre composition, EMG, and the cardiovascular response. Heart rate (HR), arterial blood pressure (BP), myoelectric signal (EMG), and intramuscular temperature (Tm) were measured during prolonged static contractions in five healthy male subjects (25-44 yrs). Two studies were performed. In study I constant EMG contraction muscle force in the first 5 s of the knee-extensor contraction was set to 20% MVC, (maximal voluntary contraction), and in the rest of the 5 min contraction the myoelectric signal was kept constant by visual feedback from an oscilloscope. In study II, (constant force contraction) two 1 h 7% MVC isometric contractions of the elbow-flexors and extensors were performed on two separate days. During the 5 min constant EMG contraction, the force fell from 20 to 11% MVC, mean BP (MAP) increased from 97 +/- 5 to 120 +/- 4 mmHg (P less than 0.01), and the EMG MSF decreased from 87 +/- 16 to 66 +/- 9 Hz (P less than 0.01). The decrease in MSF was strongly correlated to the increase in MAP (r = 0.96, P less than 0.01). The intramuscular temperature showed a small increase from 34.3 degrees C to 35.3 degrees C (P less than 0.01). During the 1 h constant force contraction involving m. triceps, MAP increased from 104 +/- 10 to 120 +/- 12 mmHg, with a simultaneous decrease in the EMG MSF from 96 +/- 11 to 70 +/- 19 Hz and an increase in the EMG amplitude (247% of the initial value). In the contractions involving m. biceps, however, both MAP and EMG MSF remained almost unchanged, but EMG amplitude increased (197% of the initial value). Very modest changes in HR were observed: 63 +/- 6 to 66 +/- 6 beats min-1 and 61 +/- 5 to 59 +/- 7 beats min-1 in the contractions involving m. triceps and m. biceps, respectively. The intramuscular temperature increased simultaneously, 1.3 degrees C and 0.7 degrees C in m. triceps and m. biceps, respectively. The results from the constant EMG contractions indicate the existence of a common 'trigger' for both the increase in BP and the decrease in EMG MSF; and the extracellular [K] is put forward as a candidate.(ABSTRACT TRUNCATED AT 400 WORDS)     

Spectral moments of mechanomyographic signals recorded with accelerometer and microphone during sustained fatiguing contractions

The aim of this study was to analyse the trends of the first three power spectral moments of the mechanomyogram (MMG) signal recorded by a microphone (MMG_MIC) and an accelerometer (MMG_ACC) during sustained contractions. MMG signals were recorded from the biceps brachii muscle in 14 healthy male subjects during a 3 min isometric elbow flexion at 30% of the maximal voluntary contraction. MMG absolute and normalised root mean square (RMS), mean power frequency (MNF), power spectral variance (Mc₂), and skewness (μ₃) were computed. For both MMG_MIC and MMG_ACC, absolute and normalised RMS and Mc₂ increased while MNF and μ₃ decreased with contraction time (P<0.001). The rates of change of RMS over time were significantly correlated (P<0.001) for MMG_MIC and MMG_ACC but not correlated for spectral moments. The coefficient of variation of RMS was higher for MMG_MIC than for MMG_ACC, while the opposite was observed for μ₃ (P<0.05). It was concluded that higher order spectral moments of the MMG signal change during sustained contraction, indicating a complex modification of the shape of the power spectrum and not just scaling of the bandwidth. This is most likely due to the additional motor unit recruitment with fatigue and to the non-linear summation of motor unit contributions to the signal. Moreover, the characteristics of MMG signals recorded with microphones and accelerometers have important differences, which should be taken into account when comparing results from different studies.Submitted to: Medical and Biological Engineering and Computing     

Non-invasive assessment of single motor unit mechanomyographic response and twitch force by spike-triggered averaging

A method for non-invasive assessment of single motor unit (MU) properties from electromyographic (EMG), mechanomyographic (MMG) and force signals is proposed. The method is based on the detection and classification of single MU action potentials from interference multichannel surface EMG signals and on the spike-triggered average of the MMG (detected by an accelerometer) and force signals. The first dorsal interosseous (FDI) and abductor digiti minimi (ADM) muscles were investigated at contraction levels of 2% and 5% of the maximum voluntary contraction (MVC) force. A third contraction was performed by selective activation of a single MU with surface MU action potential visual feedback provided to the subject. At 5% MVC, the mean (±standard error) single MU MMG peak-to-peak value was 11.0±1.8 mm s⁻² (N=17) and 32.3±6.5 mm s⁻² (N=20) for the FDI and AMD muscles, respectively. The peak of the twitch force was, at the same contraction livel, 7.41±1.34 mN and 14.42±2.92 mN, for the FDI and ADM muscles, respectively. The peak-to-peak value of the MMG was significantly different for the same MU at different contraction levels, indicating a non-linear summation of the single MU contributions. For the FDI muscle, the MMG peak-to-peak value of individual MUs was 21.5±7.8 mm s⁻², when such MUs were activated with visual feedback provided to the subject, whereas, for the same MUs, it was 11.8±3.8 mm s⁻², when the subject maintained a constant force level of 2% MVC. The method proposed allows the non-invasive assessment of single MU membrane and contractile properties during voluntary contractions.     

Electrical and mechanical response of finger flexor muscles during voluntary isometric contractions in elite rock-climbers

To determine the differences between rock-climbers and controls in finger flexor (FF) motor units (MUs) features and activation strategy, eleven climbers and ten controls volunteered for the study. After maximal voluntary contraction (MVC) assessment, five levels of isometric contractions at 20, 40, 60, 80 and 100% MVC were performed. During contractions, electromyogram (EMG) and mechanomyogram (MMG) were recorded, from which the root mean square (RMS) and mean frequency (MF) were calculated. Climbers showed significantly higher MVC. EMG RMS was statistically higher in climbers than in controls from 60 to 100% MVC. In climbers MMG RMS increased up to 80% MVC, whereas in controls it increased only up to 60% MVC. MMG MF was higher in climbers than in controls from 60 to 100% MVC (P < 0.05). EMG–MMG combined analysis revealed significant differences in MU activation strategy between the two groups. The results are compatible with a shift of climbers’ muscles toward faster MUs.     

Effect of step and ramp static contractions on the median frequency of electromyograms of back muscles in humans

The purpose of this study was to compare the electromyogram median frequency (MF) values from two contraction modes (ramp vs step) at different force levels of eight back muscles. A group of 20 healthy male subjects stood in a dynamometer with the trunk in a vertical position and performed trunk extension contractions using the displayed L5/S1 extension moment as visual feedback. The electromyogram (EMG) signals from four pairs of back muscles were collected at 4,096 Hz using active surface electrodes during two 7 s static ramp contractions ranging from 0% to 100% of the maximal voluntary contraction (MVC) and two 5 s static step contractions performed at five forces (10%, 20%, 40%, 60% and 80% MVC). The root mean square (RMS) and MF of the EMG signals corresponding to 250 ms windows were computed at each force level for both contraction modes. The RMS from the ramp contractions were significantly higher than from the step contractions in six muscles. The corresponding MF showed a significant (α=0.05) contraction mode×force interaction in four muscles. A significant contraction mode main effect was obtained in four muscles having higher MF during step than during ramp contractions. These differences were more obvious (10–15 Hz) and more frequent at the lower (10%, 20% and 40% MVC) forces. It was suggested that mechanisms not related to motor unit recruitment might influence MF in contraction modes. These unknown mechanisms contaminate any possible relationship between the MF measurements and muscle composition. Electronic Publication     

Blood pressure response to low level static contractions

Summary The present study re-examines the 15% MVC concept, i.e. the existence of a circulatory steady-state in low intensity static contractions below 15% of maximal voluntary contraction (MVC). Mean arterial blood pressure was studied during static endurance contractions of the elbow flexor and extensor muscles at forces corresponding to 10% and 40% MVC. Mean value for endurance time at 10% MVC was significantly longer for flexion [111.3 (SD 56.1) min] than for extension [18.1 (SD 7.5) min;n = 7]. At 40% MVC the difference in mean endurance time disappeared [2.3 (SD 0.7) min for elbow flexion and 2.3 (SD 0.7) min for elbow extension]. Mean arterial blood pressure exhibited a continuous and progressive increase during the 10% MVC contractions indicating that the 15% MVC concept would not appear to be valid. The terminal blood pressure value recorded at the point of exhaustion in the 10% MVC elbow extension experiment was identical to the peak pressure attained in the 40% MVC contraction. For the elbow flexors the terminal pressor response was slightly but significantly lower at 10% MVC [122.3 (SD 10.1) mmHg, 16.3 (SD 1.4) kPa] in comparison with 40% MVC [130.4 (SD 7.4) mmHg, 17.4 (SD 1.0) kPa]. When the circulation to the muscles was arrested just prior to the cessation of the contraction, blood pressure only partly recovered and remained elevated for as long as the occlusion persisted, indicating the level of pressure-raising muscle chemoreflexes. Based on blood pressure recordings obtained during the occlusion, it is suggested that the slight reduction in terminal pressor response seen in the 10% MVC elbow flexion experiment was due to a reduced chemoreflex drive characteristic of a slow twitch muscle group during prolonged low force contractions.     

Non-uniform mechanical activity of quadriceps muscle during fatigue by repeated maximal voluntary contraction in humans

To determine the non-uniform surface mechanical activity of human quadriceps muscle during fatiguing activity, surface mechanomyogram (MMG), or muscle sound, and surface electromyogram (EMG) were recorded from the rectus femoris (RF), vastus lateralis (VL), and vastus medialis (VM) muscles of seven subjects during unilateral isometric knee extension exercise. Time- and frequency-domain analyses of MMG and of EMG fatigued by 50 repeated maximal voluntary contractions (MVC) for 3?s, with 3-s relaxation in between, were compared among the muscles. The mean MVC force fell to 49.5 (SEM 2.0)% at the end of the repeated MVC. Integrated EMG decreased in a similar manner in each muscle head, but a marked non-uniformity was found for the decline in integrated MMG (iMMG). The fall in iMMG was most prominent for RF, followed by VM and VL. Moreover, the median frequency of MMG and the relative decrease in that of EMG in RF were significantly greater (P?) than those recorded for VL and VM. These results would suggest a divergence of mechanical activity within the quadriceps muscle during fatiguing activity by repeated MVC.     

Comparison of maximal unilateral versus bilateral voluntary contraction force

The aim of the present study was to determine whether the difference in maximal voluntary contraction (MVC) forces between unilateral (UL) and bilateral (BL) contractions could be due to a difference in the timing of MVC peak force production between legs during BL MVC, or due to the maximal voluntary activation level (VAL) in each leg between UL and BL MVC. Thirteen active men (28 ± 6 years) volunteered to participate in an experimental session requiring the performance of MVC with right, left and both knee extensor muscles. An ergometer equipped with two strain gauges was used to assess the timing of MVC peak force production of each leg during BL MVC. Transcutaneous electrical stimulation was used to investigate VAL and electromyography (EMG) activity. The present results showed that the sum of the right and left UL MVC was higher compared to BL MVC force, i.e., a BL deficit of force. The timing of MVC peak force production of each leg during BL MVC was not different, but BL MVC force was lower than the sum of BL MVC_R and BL MVC_L force (i.e., maximal force production of each leg during BL MVC). No changes of EMG activity, M-wave amplitude, VAL, and peak doublet between UL and BL contractions were observed. The difference found in the production of force during BL MVC cannot be explained by the timing of force production of each leg.     

Mechanomyographic responses during voluntary ramp contractions of the human first dorsal interosseous muscle

The aim of this study was to examine the mechanomyogram (MMG) and force relationship of the first dorsal interosseous (FDI) muscle as well as the biceps brachii (BB) muscle during voluntary isometric ramp contractions, and to elucidate the MMG responses resulting from the intrinsic motor unit (MU) activation strategy of FDI muscle with reference to the MMG of BB muscle. The subjects were asked to exert ramp contractions of FDI and BB muscle from 5% to 70% of the maximal voluntary contraction (MVC) at a constant rate of 10% MVC/s. In FDI muscle, the root-mean-squared amplitude (RMS) of the MMG decreased slowly with force up to 21%, and then a progressive increase was followed by a relatively rapid decrease beyond 41% MVC. The RMS/%MVC relationship in BB muscle consisted of an initial slow increase followed by a rapid increase from 23% MVC and a progressive decrease beyond 61% MVC. With respect to the mean power frequency (MPF), FDI muscle demonstrated no obvious inflection point in the MPF/%MVC relationship compared with that in BB muscle. Namely, the MPF of FDI muscle increased linearly through the force levels exerted. In contrast to FDI muscle, the MPF/%MVC relationship in BB muscle was decomposed into four specific regions: (1) a relative rapidly increase (<34% MVC), (2) a slow increment (34–53% MVC), (3) a temporary reduction (53–62% MVC), and (4) a further rapid increase (>62% MVC). The different MMG responses between FDI and BB muscles are considered to reflect the fact that the MU activation strategy varies among different muscles in relation to their morphology and histochemical type. Namely, the rate coding of the MUs plays a more prominent role in force production in relatively small FDI muscle than does MU recruitment compared with their respective roles in the relatively large BB muscle.     

Sonomyographic responses during voluntary isometric ramp contraction of the human rectus femoris muscle

This paper aims to investigate the relationship between torque and muscle morphological change, which is derived from ultrasound image sequence and termed as sonomyography (SMG), during isometric ramp contraction of the rectus femoris (RF) muscle, and to further compare SMG with the electromyography (EMG) and mechanomyography (MMG), which represent the electrical and mechanical activities of the muscle. Nine subjects performed isometric ramp contraction of knee up to 90% of the maximal voluntary contraction (MVC) at speeds of 45, 22.5 and 15% MVC/s, and EMG, MMG and ultrasonography were simultaneously recorded from the RF muscle. Cross-sectional area, which was referred to as SMG, was automatically extracted from continuously captured ultrasound images using a newly developed image tracking algorithm. Polynomial regression analyses were applied to fit the EMG/MMG/SMG-to-torque relationships, and the regression coefficients of EMG, MMG, and SMG were compared. Moreover, the effect of contraction speed on SMG/EMG/MMG-to-torque relationships was tested by pair-wise comparisons of the mean relationship curves at different speeds for EMG, MMG and SMG. The results show that continuous SMG could provide important morphological parameters of continuous muscle contraction. Compared with EMG and MMG, SMG exhibits different changing patterns with the increase of torque during voluntary isometric ramp contraction, and it is less influenced by the contraction speed.     

The influence of torque and velocity on erector spinae muscle fatigue and its relationship to changes of electromyogram spectrum density

The influence of contraction force and velocity during isokinetic contractions on the development of fatigue in the erector spinae muscle was studied. Seven male subjects performed a series of 250 contractions at 25% and 50% of their isometric maximal voluntary contraction (MVC) at 40 and 80°·s^–1. Fatigue defined as a decrease of the contractile capacity of the muscles was studied by means of a 15-s maximal test-contraction following the exercise. Both the initial force and the force decrement during the test-contraction were studied. Surface electromyogram (EMG) signals of the main tracts of the erector spinae muscle were recorded. The frequency content was studied by calculating the zero-crossing rate for the signals obtained during dynamic contractions and by means of fast Fourier transformation for the test contraction. After the 50% MVC dynamic contractions the initial force during the postexercise test-contraction was significantly lower than after the 25% MVC contractions. No significant influence of contraction velocity on fatigue development was found. The force decrement during the test-contraction did not depend on the experimental conditions. The EMG amplitude indicated that the subjects were better able to relax their muscles during the counter movement (flexion) at high forces and high velocities compared to the other experimental conditions. The frequency content of the EMG signals during the dynamic contractions and the postexercise test-contraction showed only very weak relationships with fatigue. Therefore, spectrum EMG parameters as determined in the present study do not seem suitable as indicators of muscle fatigue as a consequence of dynamic contractions of trunk extensor muscles.