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丛枝菌根及其在中药材栽培中的应用 总被引:1,自引:0,他引:1
本文对丛枝菌根的来源、作用、研究现状以及对丛枝菌根在中药栽培中的应用情况进行了介绍,指出借鉴作物中丛枝菌根真菌研究的诸多成果并将其运用于中药研究具有重要意义的。 相似文献
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目的:研究丛枝菌根侵染强度对三七生长和药效成分皂苷的影响;方法:在大田条件下,对三七种子接种丛枝菌根真菌Glomus mosseae,生长二年后,随机采集100株三七,分株测定菌根侵染强度、地下部生物量和皂苷含量;结果:随着丛枝菌根侵染强度的增加,三七地下部生物量、人参皂苷Rd和四种皂苷总含量均出现明显地先增加而后出现下降趋势。同时,随着丛枝菌根侵染强度的增加,三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1、人参皂苷Rd以及这四种皂苷的总产量均出现明显地先增加后下降的趋势。结论:丛枝菌根侵染强度与三七地下部生物量、皂苷含量及皂苷产量存在显著的相关性,具有低侵染增加,高侵染转而降低的现象。丛枝菌根适度侵染时三七有最大的生物量和最高的皂苷含量及皂甙产量。 相似文献
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目的比较接种和不接种丛枝菌根真菌对滇重楼根茎中重金属元素铅、镉、砷、铜、汞的影响。方法通过室温盆栽接种试验方法,采用微波消解-原子吸收光谱法测定并比较收获滇重楼根茎中重金属的差异。结果不同丛枝菌根真菌对滇重楼根茎中重金属量的影响存在差异。接种丛枝菌根真菌能部分地降低其根茎中重金属含有量,所测量值均低于《药用植物及制剂进出口绿色行业标准(WM2-2001)》和《中国药典》2010年版相应的重金属限量标准。结论人工接种丛枝菌根真菌可保证滇重楼临床用药的安全性。 相似文献
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同位素~(13)C标记的磷脂脂肪酸(PLFA)法,可以追踪有微生物参与的碳循环过程,并且揭示微生物在此过程中的功能,PLFA法在微生物生态研究中已经被广泛应用。通过查阅国内外相关文献,归纳总结了~(13)C标记的PLFA法在微生物介导的植物-土壤碳循环中的应用,包括植物残体的微生物分解、土壤激发效应、丛枝菌根、光合作用产物的微生物利用及其在甲烷氧化菌、浮游植物等特定微生物上的应用。在此基础上,对其在中药生态种植研究领域进行展望,包括丛枝菌根增加药用植物抗逆性、缓解连作障碍和非药用部位堆肥还田。 相似文献
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接种泡囊-丛枝菌根剂对人参产量的影响 总被引:4,自引:0,他引:4
本文报道了三年生人参苗接种泡囊-丛枝菌根剂的试验。结果表明人参对泡囊-丛枝菌根具有较强的依赖性,自然侵染率约为13.1%,接种菌根剂后侵染率提高至35.5%,能显著提高人参植株的存活率,以及产量和单支重。 相似文献
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目的:研究贵州地区栽培及野生半夏丛枝菌根及丛枝菌根真菌.方法:通过染色镜检法观察栽培及野生半夏根系,通过湿筛-蔗糖离心法分离半夏根围土壤中丛枝菌根真菌孢子,并对照Schenck & Pérez及一些近期分类文献的描述,鉴定到种.结果:半夏具有典型从枝菌根结构,从贵州省6个野生及栽培半夏根围土壤采集地中共分离到隶属3属的21种丛枝菌根真菌孢子,鉴定到种的有隶属3属的7种,其中球囊霉属5种,巨孢囊霉属1种,盾巨孢囊霉属1种,分别是摩西球囊霉、根内球囊霉、沙漠球囊霉、黑色球囊霉、聚丛球囊霉、白色巨孢囊霉、粟色盾巨孢囊霉.其中粟色盾巨孢囊霉为我国新纪录种,且为毕节地区栽培半夏的优势种.结论:贵州省野生及栽培半夏中丛枝菌根真菌具有丰富多样性,孢子种类、优势种均差异较大,如白色球囊霉仅出现于栽培半夏根围土壤中,黑色球囊霉仅出现于野生半夏根围土壤中,而摩西球囊霉和根内球囊霉在2种土壤中均有出现,且毕节地区半夏根围土壤中有专属性较强的丛枝菌根真菌,可为栽培半夏退化提供新的研究思路. 相似文献
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目的:研究浙麦冬根际菌根真菌多样性以及种植地土壤性状。方法:用湿筛法获得麦冬根际土壤的丛枝菌根真菌孢子,计数孢子密度、物种丰富度及各物种的出现频率;分析浙麦冬种植地土壤性状及金属含量和农药残留。结果:浙麦冬根际土壤中丛枝菌根资源丰富,孢子密度平均824.33个/100g土壤,球囊霉属、巨孢囊霉属和无梗囊霉属为优势类群;菌种丰富度平均为5。丛枝菌根真菌孢子的分布呈明显的季节性变化。种植土壤含有丰富的钙、镁、锌、铜、铬等金属元素,无农药残留。结论:浙麦冬根际含有丰富的球囊霉属、巨孢囊霉属和无梗囊霉属等菌根真菌,其分布具有明显的季节性;根际土壤含有丰富的钙、镁、锌、铜、铬等金属元素。 相似文献
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药用植物与丛枝菌根真菌的选择性侵染研究 总被引:1,自引:0,他引:1
目的 观察丛枝菌根真菌(AMF)对植物的侵染过程及其规律.方法 采用数码显微成像系统对侵染结构进行了观察,测定6种AMF对4种药用植物的侵染率;采用湿筛法对AMF孢子进行分离并拍照.结果 试验发现不同种AMF对同种植物的侵染率有较大差别,不同植物对相同菌种的选择也有较大差别.结论 摩西球囊霉、根内球囊霉等AMF对上述药用植物均具有较高的侵染率,因此可作为药用植物的丛枝菌根研究首选菌种. 相似文献
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《结合医学学报(英文版)》2014,(3)
Objective: Medical imaging techniques(MITs) provide various useful methods for acupuncture and moxibustion(AM) research.This article aims at summarizing and analyzing the application and developing trend of MITs in AM research at home and abroad, so that researchers could bring in some inspirations and evidence for further studies.Methods: Through Google Scholar searching, MIT- and AMrelated literature was selected and reviewed.It covers meridiansacupoints fundamental research, action mechanisms and clinical efficacy studies.Various MITs including nuclear imaging, functional magnetic resonance imaging(fMRI), optical and ultrasound(OU) imaging were analyzed in details.The current achievements of MIT application were summarized and analyzed, and the developing trend was evaluated.Results:(1) The application of MITs in AM research has been experienced the transformation from structural and macroscopic imaging to functional and molecular imaging; positron emission tomography(PET) with MRI marks the highest level of MITs.Besides, fMRI was used most frequently and studied most deeply, single-photon emission computed tomography(SPECT) and PET seemed have attracted more attention, and OU functional and molecular imaging were new, but promising.Optical molecular imaging might become the most promising method in AM central and peripheral mechanism research.(2) MIT research proved that Qi flows along meridians, and confirmed the effects of AM therapy.Studies of more in-depth mechanisms explaining central nervous system involved in AM analgesia and "DEQI" were carried out.(3) The subjects of functional brain imaging(fBI) studies have been gradually inclined to patients, not healthy volunteers as previously.The methods were still mainly based on observing immediate effects of single acupoint stimulation, and the experimental repeatability was poor which was affected by complicated factors.Conclusion: MITs are playing an important role in AM research.fBI and molecular imaging would contribute greatly to the research of AM mechanisms and meridian essence theory. 相似文献
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蒙古黄芪与膜荚黄芪种子形态特征及其鉴别方法的研究 总被引:3,自引:0,他引:3
目的为黄芪种子鉴定提供方法,为按中药材GAP原则制定相关标准操作过程(SOP)提供基础研究资料。方法采用肉眼直接观察、光学显微镜观察和电子显微镜进行扫描观察,比较蒙古黄芪和膜荚黄芪两种药用黄芪的种子形态特征和微观结构;通过萌发试验,比较二者的差异。结果通过肉眼和光学显微镜观察,两种黄芪种子的形态差异不明显,在电子显微镜下观察,两种黄芪种子的萌发孔形状、种脐和种皮的微观结构有明显差异;蒙古黄芪较膜荚黄芪种子硬实率高,萌发不整齐,萌发高峰滞后。结论在电子显微镜下能够对两种黄芪种子准确地做出鉴定,种脐、萌发孔、种皮的微观结构可作为鉴别两种黄芪种子的指标;种子硬实率和萌发动态规律可用于两种黄芪种子的辅助鉴别。 相似文献
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目的;观察中西医结合治疗小鼠柯萨奇B3(CVB3)病毒性心肌炎(VMC)的疗效。方法:腹腔接种CVB3病毒液以建立VMC模型。随机分为对照、黄芪、牛磺酸和辅助产Q10(CoQ10)单独用药组及黄芪分别加牛磺酸、CoQ10治疗组以及三药联合治疗组。观察了7组在死亡率、心电图、心肌中CVB3RNA含量及心肌形态学改变差别。结果:黄芪联合牛磺酸和CoQ10治疗可明显降低小鼠的死亡经及急性期异常心电图的必 相似文献
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《结合医学学报(英文版)》2023,21(4):354-360
Kaiy (medieval cautery) is an ancient method of heat therapy in traditional Persian medicine (TPM). Some of its important applications have been neglected during the medical revolution. Meanwhile, different treatment modalities that incorporate heat, including moxibustion, have progressed in traditional Chinese medicine. In this study, we reviewed the main TPM textbooks that were written specifically in the field of kaiy. We considered the traditional teachings in the context of contemporary information, gathered from the scientific literature about moxibustion and modern cauterization. Some surgical therapeutic indications of kaiy (e.g., debridement and coagulative procedures) have been advanced by the innovation of electro-cauterization. However, those therapeutic applications that were based on the TPM humoral theory for relieving body coldness or myofascial pains—which are similar to moxibustion usages—have not received the same attention. Apart from the broad similarities of kaiy and moxibustion as thermal therapies with similar indications, there is a striking correspondence between kaiy point mapping and acupoints. Therefore, further research on different kaiy aspects is recommended.Please cite this article as: Jaladat AM, Alizadeh Vaghasloo M, Atarzadeh F, Ayati MH, Kazemi AH, Akin E, Hashempur MH. Similarities and differences between kaiy in Persian medicine and moxibustion in Chinese medicine. J Integr Med. 2023; 21(4):354–360. 相似文献
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4种AM 真菌对苍术根茎生长及其挥发油成分的影响 总被引:1,自引:0,他引:1
目的:研究4 种AM 真菌对苍术根茎生长及其挥发油成分的影响。方法:采用盆栽试验,接种AM 菌剂,测定苍术地下部分生长状况和根茎挥发油成分的含量。结果:不同AM 真菌对苍术根茎的侵染率不同,接种AM 真菌能显著降低苍术根茎的折干率,而对根茎挥发油中5 种主要成分含量均无明显影响。结论:AM 真菌与植物之间具有选择性。不同AM 真菌对苍术地下部分的营养生长存在差异,但对苍术挥发油的品质无显著性影响(P>0.05)。 相似文献
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黄芪对自发性高血压大鼠动脉压力反射敏感性的影响 总被引:15,自引:0,他引:15
目的:评价黄芪的降压效应及其在降压过程中对压力反射敏感性(BRS)的影响。方法:12周龄自发性高血压大鼠(SHR)19只,采用不同剂量的黄芪注射液腹腔注射给药,每天1次,共8周。并连续定时观察血压变化,8周后测定BRS,并比较用药各组与不用药组血压、BRS的差异。结果:未用药组SHR血压自第3周开始升高(P<0.01),而给药的各组SHR血压无变化;经黄芪处理后的各组SHR的BRS明显比自然饲养的SHR高(P<0.01或P<0.05)。结论:黄芪可以改善SHR的BRS。 相似文献