重症急性胰腺炎的早期预测及综合疗法

目的 探讨重症急性胰腺炎（SAP）的早期预测与治疗。方法 本组125例均行手术治疗，1997年3月～2000年3月71例SAP，按照APACHE Ⅱ标准诊断为SAP，2001年3月-2004年3月54例急性胰腺炎（AP）先行抗坏血酸（ascorbic acid，AA）水平预测SAP，并行综合疗法。其中50例（93％）发展成SAP作为治疗组。结果 研究表明，治疗组能减少并发症，降低死亡率。缩短住院天数。结论 依照AA预测SAP及早行综合疗法非手术治疗SAP，有潜在的临床应用价值。     

重视重症急性胰腺炎继发感染的早期诊断与治疗

目的：评估重症急性胰腺炎（severe acute pancreatitis，SAP）的早期诊断与治疗。方法：2000年1月-2004年12月对我院16例SAP继发感染的病人，根据治疗方法不同分为两组：手术治疗组和非手术治疗组。对两组的病死率及相应并发症发生率进行回顾性分析。结果：SAP继发感染手术组病死率为8．3％（1例），并发症为25％（3例）；非手术组病死率为50％（2例），并发症为100％（2例）。结论：提高对SAP继发感染早期诊断的认识和及时手术治疗。有望提高SAP继发感染的诊治水平。     

重症急性胰腺炎32例临床诊治分析

目的：提高对重症急性胰腺炎（SAP）的认识,探讨重症胰腺炎的有效治疗方案。方法：回顾性分析我院2002～2007年收治的32例重症急性胰腺炎的临床资料。结果：本组32例,治愈27例,死亡5例。行非手术治疗25例,死亡4例;行手术治疗7例,死亡1例。休克和多器官功能衰竭是死亡的主要原因。结论：重症急性胰腺炎急性反应期宜行综合措施的非手术治疗。暴发性急性胰腺炎,有胆道梗阻或胆管炎的患者应及时手术治疗。     

复方丹参在重症急性胰腺炎非手术治疗中的作用

目的：探讨复方丹参注射液综合性非手术治疗重症急性胰腺炎（SAP）的疗效。方法：对1997年5月至2000年2月收治的SAP28例，随机分为两组，对照组13例应用抗休克，抑制胰液分泌，全胃肠外营养，联合使用抗生素的综合治疗，治疗组15列在此基础上加用复方丹参注射液，结果：对照组死亡例（7．69％），严重并发症5例（38．5％），中转手术2例（15．4％），治疗组并发症1例（6．67％），两组在死亡＋并发症方面有显著性差异（P＜0．05），结论：复主丹参注射液是SAP综合性非手术治疗中不可缺少的药物之一。     

非手术治疗39例重症急性胰腺炎临床分析

目的探讨重症急性胰腺炎（SAP）非手术治疗的方法及效果。方法回顾分析我科1998年9月～2004年3月采用非手术方法治疗SAP39例的临床效果。结果本组病例中，4例内科治疗无效转手术治疗（其中1例死亡），3例死亡，32例治愈，治愈率82．05％（32／39）。结论对SAP患者采取包括中药在内的综合性非手术治疗具有较好的治疗效果。     

重症急性胰腺炎治疗干预研究

目的探讨重症急性胰腺炎（Severe acute pancreatitis，SAP）手术干预的指征和方法。方法回顾性分析2000年1月-2006年2月治疗出院的38例SAP病例，包括7例暴发性胰腺炎（Fulminant acute pancreatitis，FAP），其中非手术治疗28例（73.7％）。手术干预治疗10例（26.3％）。结果总存活率73．7％（28／38），非手术治疗组71．5％（20／28），手术组80％（8／10）。结论个体化综合性治疗SAP是安全有效的，但当继发明显感染、胆道梗阻或出现FAP时应结合重症加强治疗及时采取个体化手术。     

76例急性重症胰腺炎治疗的临床体会

目的：探讨急性重症胰腺炎（SAP）的诊断、治疗原则及方法,以提高患者的治愈率,减少病死率及并发症的发生。方法：回顾性分析我院76例SAP的诊断和治疗方法及其对病死率和并发症发生率的影响。结果：76例中,治愈58例（76．3％）、死亡18例（23．7％）。手术治疗组24例,死亡8例（33．3％）;非手术治疗组52例,死亡10例（19．2％）。本病的预后与早期诊断及治疗方法的选择密切相关。结论：彩色B超、CT检查有助于SAP的早期诊断,并对急性重症胰腺炎患者在综合治疗的基础上实施个体化治疗。可提高治愈率。     

重症急性胰腺炎60例综合治疗分析

目的 总结重症急性胰腺炎的临床综合治疗效果。方法 回顾性分析1990～2003年本科收治的60例重症急性胰腺炎，在治疗上分两个阶段。第一阶段1990～1997年以手术治疗为主（手术组）28例，第二阶段1998～2003年以非手术治疗为主（保守组）32例。结果 手术组：治愈20例，死亡8例，治愈率71.4%;保守组：治愈30例，死亡2例，治愈率93.8%。结论 早期手术并不能降低病死率；以非手术治疗为主的综合治疗能提高重症急性胰腺炎的治疗效果。     

内科综合治疗重症胰腺炎36例临床分析

重症急性胰腺炎（SAP）是指急性胰腺炎伴脏器功能衰竭,或出现胰腺坏死、脓肿或假性囊肿等局部并发症者,或两者皆有.它是一种危重急腹症,病情凶险,并发症多,病死率高.目前对SAＰ的治疗尽管意见不一,但早期非手术治疗,后期手术处理胰腺或胰周坏死感染的“个体化方案和综合治疗体系”正被广泛接受.本院自2000年10月～2005年3月间内科综合治疗36例SAP,现总结如下. 　　……     

18例急性坏死性胰腺炎的保守治疗

目的：探讨急性坏死性胰腺炎（SAP）保守治疗的疗效。方法：回顾性总结1995－2000年收治的18例SAP患者行早期非手术治疗的结果和需着重注意的事项。结果：18例病人死亡2例,需后期行感染灶引流的仅3例,平均住院时间为50．2天。结论：SAP患者早期宜行严密监测下的保守治疗,初期治疗要强调改善胰腺的微循环。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.