��������ɳ����������������������Ʊ�������

??OBJECTIVE To select excipients and optimize preparation for the ambroxol hydrochloride/levosalbutamol sulfate dry powder inhalation. METHODS Drug and different excipients in the same ratio were prepared by spray drying to obtain a certain particle size as drug particles or carriers for dry powder inhalation, respectively. Study and compare the parameters of powders of the dry powder inhaler, such as the angle of repose, moisture content, and emptying rate, etc, to screen the excipient of the vectors. Afterward, Box-Behnken design method was adopted to optimize of preparation parameters. RESULTS When lactose-high branched cyclodextrin compounds were used as composite carriers, their powder properties meet the requirements of dry powder inhaler, such as the repose angle was 39.69 degrees, the bulk density was 0.37 g??mL^-1, the tap density was 0.66 g??mL^-1, the evacuation rate of drug-carrier powders in capsules was 93.12%, and the water content was 0.10% and the simulated lung deposition rate was 14.63%. The preparation parameters, such as sample concentration, inlet air temperature, nozzle diameter parameters, theirs values were 50 mg??mL^-1, 110 ??, 1 mm, respectively, were opmized by Box-Behnken design method, which could obtain the carrier particle size of 30.33 ??m. CONCLUSION Lactose-high branched cyclodextrin complexes can be used as carrier for levosalbutamol sulfate-ambroxol hydrochloride compound dry powder inhaler, which could meet the requirements of dry powder inhaler by spray-drying preparation method.     

�ǵ����-ЧӦ�淨�Ż�������-�DZ���-��-��������������Ʊ�����

??OBJECTIVE To prepare the inclusion complex of Lignum dalbergia odorifera oil with hydroxyl-??-cyclodextrin(HP-??-CD), and to optimize the preparation process of it. METHODS The inclusion complex was prepared by the stirring-freeze-dry method. The preparation process was optimized by central composite design-response surface method (CCD-RSM),with the colligation score which was calculated by the yield of inclusion, the utilization rate of volatile oil and the content of trans-nerolidol as index. The inclusion complex was verified by phase-solubility method, DSC,UV and microscopical identification. RESULTS The optimum inclusion technology was: inclusion solvent 5% ethanol, stirring rate 500 r??min^-1, HP-??-CD to volatile oil 33??1, inclusion temperature 42 ??,inclusion time 2.5 h. The formation of inclusion complex can change the solubility, optical and thermodynamic properties of volatile oil. CONCLUSION The preparation process of inclusion complex of Lignum dalbergia odorifera oil with HP-??-CD optimized by CCD-RSM is reasonable and feasible, and provide a reliable experiment basis for its application.     

�������ܳ����õ�º��Ĵ����Ż��о�

??OBJECTIVE To optimize the formulation of iodoform paste for root canal filling of deciduous teeth. METHODS Using multi-indicators comprehensive scoring method, which takes the delicate degree, lubricity, difficulty of root canal filling, and difficulty of removal as performance indicators, the matrix type was screened, and the formulation of paste was optimized by orthogonal experimental design. The content of iodoform in the optimized paste was determined by UV spectrophotometry. RESULTS Dimethicone was chosen as the paste matrix by comprehensive scoring of indicators. The results of orthogonal test showed that the ratio of iodoform and zinc oxide was the main factor affecting the performance of the paste, and the optimal formulation of paste was as follows:the ratio of iodoformto zinc oxide 3??7, clove oil 0.5%, and the ratio of powder to liquid 70??30. The linear range of the calibration curve for iodoform in the optimal paste was 48.75-146.22 ??g??mL^-1, A=6.781 3C-0.051 6(r=0.999 6); and the average recovery rate was 99.83% (n=9). CONCLUSION The optimal iodoform paste has a simple preparation, fine and smooth for root canal filling of deciduous teeth. The assay method is accurate and reliable for the quality control of iodoform paste.     

AB-8�����֬������ɫ������ɫ�յĹ����о�

??OBJECTIVE To study the purification process of anthocyanins from purple Solanum tuberosum by AB-8 macroporous resin combined with alcohol precipitation method. METHODS Using River John Blue genotype purple Solanum tuberosum as raw material, purple Solanum tuberosum anthocyanin crude extract was obtained by leaching method. Then, the purification process of the crude extract was studied by using static adsorption (stirring, loading concentration, loading pH, eluent concentration, eluent pH) and dynamic adsorption (loading flow rate and elution flow rate), respectively, taking adsorption rate, resolution rate and color value as the evaluation indexes. On the basis of the single factor experiment, orthogonal optimization analysis was carried out to get the final optimized process condition. RESULTS The optimum conditions for the purification of AB-8 macroporous resin were as follows stirring, loading concentration 0.500 mg??mL^-1, loading pH 2, ethanol concentration 70% and elution pH 1. The column flow rate was 1 mL??min^-1, and the elution flow rate was 1 mL??min^-1. Under this condition, the color value of anthocyanin was able to reach 75.40, which was 8.43 times higher than that before purification. CONCLUSION The purification method has high feasibility and can improve the purity and color value of anthocyanins, which laids a foundation for the market development and application of anthocyanins of purple Solanum tuberosum.     

�᳦��������ζ���΢���Ʊ������о�

??OBJECTIVE To study the preparation process of pH-dependent delayed colon targeting pellets of astragalus polysaccharide. METHODS Colon targeting agents of APS pellets were produced by extrusion-spheronization and fluid bed coating method and the best preparation technology was chosen by response surface optimization method. RESULTS The ratio of pill core:APS powder-avicel-tannic acid-carboxymethylcellulose sodium was 25??15??8??2, the wetting agent was water, the rate of extrusion was 60 r??min^-1, the rate of spheronization was 1 400 r??min^-1, and the time of spheronization was 4 min. The best fluid bed coating condition was as follows the fan frequency was 29.50 Hz, the pressure of spray gun was 0.70 kg??cm^-2, the rate of coating flow was 3 mL??min^-1, and the colon coating weight was 15%.The release degree of pill for simulated gastric fluid in 2 h was 0%, The release degree of pill for artificial intestinal fluid in 3 h was less than 5%. The release degree of pill for artificial colon fluid in 2 h was release completely. CONCLUSION The preparation method can be qpplied to the preparation of APS pellets, it??s simplicity of operator and had good reproducibility, it can be applied to the industrial production.     

扎那米韦吸入粉雾剂理化性质及其初步稳定性考察

 目的 研究扎那米韦吸入粉雾剂的理化性质并考察其稳定性。方法 采用喷雾干燥法制备扎那米韦吸入粉雾剂,考察其吸湿性及临界相对湿度;观察其粒径分布、粉末形态;热重（TGA）法测定其水分;检测其排空率和沉积率;对制剂进行加速实验和长期实验以考察其稳定性。 结果 所得扎那米韦制剂的临界相对湿度为67%,平均空气动力学径小于5 μm,样品所含水分小于5%,排空率均大于90%,肺部沉积率均大于30%;稳定性实验中各指标在观察期内无明显变化。 结论 所制扎那米韦吸入粉雾剂适合肺部吸入给药,且具有较好的稳定性。     

�ߴ�����΢��-�����������Ʊ������������о�

??OBJECTIVE To optimize the preparation process of osthole microcapsules-temperature-sensitive gel and set up its quality standard.METHODS Using gelling temperature as the indicator,P407, P188 and the concentration of propylene glycol were investigated by single factor test,and orthogonal experiment was conducted to optimize the preparation process of osthole microcapsules-temperature-sensitive gel.Osthole content was determined by HPLC.The quality standard of osthole microcapsules-temperature-sensitive gel was established.RESULTS The optimal formulation of osthole microcapsules-temperature-sensitive gel was as followsP407-P188-propylene glycol=18%??1%??15%.Osthole contentin the osthole microcapsules-temperature-sensitive gel should not be less than 31.77 ??g??mL^-1, and the gelling temperature should be 36-37 ??.CONCLUSION Osthole microcapsules-temperature-sensitive gel prepared in this study has reasonable composition,simple preparation process, and stable quality standards,indicating a hopeful application prospect.     

温性经筋通贴膏醇提和制剂工艺优化研究

目的优化温性经筋通贴膏的醇提工艺和制剂配方工艺,使其发挥更好的临床疗效。方法采用L₉(3⁴)正交试验法,以乙醇浓度,乙醇倍量,提取时间,提取次数为考察因素,以干膏得率为评价指标,优选最佳提取工艺条件;采用混合型骨架,用L₉(3⁴)正交试验法,以NP-700、高岭土、甘羟铝、酒石酸为考察因素,以成型凝胶贴膏的初黏力、持黏力、剥离强度、综合感官为评价指标,优选出制剂最佳配方。结果温性经筋通贴膏最佳醇提工艺是以6倍量的70%乙醇提取2次,每次2 h;优化的制剂配方是NP-700∶高岭土∶甘羟铝∶酒石酸=100∶80∶8∶0.8。结论优选出的工艺稳定可行,适合制剂生产操作,可作为温性经筋通贴膏的提取工艺和制剂配方工艺。     

��ѹ����糡��������ȡ¹���е����ʡ����ǵĹ����Ż�

??OBJECTIVE To optimize the extraction proces of protein and polysaccharides from Antler Velvet using high intensity pulsed electric fields. METHODS With water as solvent, electric field intensity, pulse number and solid-liquid ratio as investigation parameters, an L₉(3⁴) orthogonal test was used to determine the optimal process for simultaneous extraction of protein and polysaccharides from Antler Velvet. RESULTS When the extraction by high intensity pulsed electric fields was conducted at the solid-liquid ratio of 1??30 g??mL^-1 and electric field intensity of 20 kV??cm^-1, with pulse number of 10, the protein extraction rate reached 12.32%, and polysaccharides extraction rate could reach 3.25%. CONCLUSION The optimized process can effectively and simutaneously extract protein and polysaccharides from Antler Velvet.