蒙药别冲-15的抗炎作用研究

目的:研究别冲-15的抗炎作用。方法:用二甲苯致小鼠耳肿胀,鸡蛋清致小鼠足肿胀,二甲苯致小鼠耳毛细血管通透性增高模型,观察别冲-15对小鼠耳肿胀、足肿胀以及耳毛细血管通透性增高的影响。结果:别冲-15明显抑制小鼠耳肿胀,足肿胀和耳毛细血管通透性增高。结论:别冲-15有明显的抗炎作用。     

蒙药托雷-15治疗类风湿性关节炎

运用蒙药托雷-15治疗30例类风湿性关节炎患者，治愈率达到86.7%。     

HPLC测定阿拉探阿如日-15（色日刀-15味散）中胡椒碱的含量

目的：测定阿拉探阿如日-15味散中胡椒碱的含量。方法：采用HPLC法测定阿拉探阿如目-15味散中胡椒碱的含量。结果：胡椒碱峰的分离效果较好,平均回收率为100．49％,RSD为1．7％。结论：本方法准确、重现性好,可作为阿拉探阿如日-15味散的含量控制方法。     

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??OBJECTIVE To isolate and purify 2??,3??,24-trihydroxy-12-alkene-28-ursolic acid from the roots of Actinidia eriantha Benth, establish its determination method, and compare the contents in different extracted parts and samples from different sources. METHODS An HPLC-PDA method was established for the content determination. The contents of 2??,3??,24-trihydroxy-12-alkene-28-ursolic acid in the roots of Actinidia eriantha Benth from five different sources and different extracted parts were determined by standard curve method. RESULTS The content of 2??,3??,24-trihydroxy-12-alkene-28-ursolic acid was higher in the samples from Shouning county of Fuzhou city and Yunhe county of Lishui city while lower in those from Yongjia county of Wenzhou city and Lishui city. And in different extracted parts, the content was the highest in dichloromethane part, lower in ethanol part, and the lowest in methanol part. CONCLUSION The method is stable and accurate with good reproducibility and can be used for the determination of 2??,3??,24-trihydroxy-12-alkene-28-ursolic acid in the roots of Actinidia eriantha Benth. The content of 2??,3??,24-trihydroxy-12-alkene-28-ursolic acid is different in Actinidia eriantha Benth samples from different sources, which is the highest in dichloromethane part.     

蒙药托雷-15治疗类风湿性关节炎30例

类风湿性关节炎为临床常见病。笔者近几年来用自制的治疗类风湿性关节炎的专方托雷 - 1 5治疗本病 ,效果良好 ,现报告如下。一般资料本组观察治疗 30例 ,其中男性 1 7例 ,女性 1 3例 ;1 8～ 35岁者 6例 ,36～ 5 5岁者 1 6例 ,5 6～ 75岁者 8例 ;病程 1～ 30年。患者化验类风湿因子均为阳性或强阳性 ,60 %的患者有小关节不同程度的变形或肿胀、红肿 ,压痛明显。治疗方法配方 :草乌、诃子、珊瑚、沉香、磁石、苦参等 1 5种药物加工成药粉 ,过 1 0 0～ 1 2 0目筛 ,打成黄豆大小水丸备用。治疗时视病情 ,重者早晚服 7～ 1 3粒 ,温水送服 ;轻者晚…     

15-PGDH�յ�ҩ����ת�����ٰ�MCF-7/ADRϸ����ҩ���ü�����

??OBJECTIVE To examine the expression of 15-hydroxyprostaglandin dehydrogenase(15-PGDH) in human multidrug-resistant breast cancer line MCF-7/ADR and to explore the reversal effect and mechanism of 15-PGHD induction drugs on MCF-7/ADR cells. METHODS The RT-PCR and Western blot were used to detect 15-PGDH, COX-2 mRNA and protein expression in MCF-7 and MCF-7/ADR cells. PGE₂ levels in supernatant of cells were determined by ELISA assay. Anti-proliferation effect and chemotherapy sensitivity to ADM of 15-PGDH induction drugs (indomethacin, ibuprofen and pioglitazone, dexamethasone) on breast cancer cells were assayed by MTT method. Cell apoptosis was detected by Hochest 33258 stain assay. RESULTS Compared with MCF-7 cells, the 15-PGDH expression was significantly decreased, COX-2 expression was significantly increased and PGE₂ levels in cell supernatant were increased in MCF-7/ADR cells. 15-PGDH induction drugs (indomethacin, ibuprofen and pioglitazone, dexamethasone) increased 15-PGDH expression or both reduced COX-2 expression, and finally reduced PGE₂ levels in MCF-7/ADR cells. Effect of chemosensitivity and apoptosis induction of ADM was enhanced and multidrug resistance was partially reversed when co-treated with 15-PGDH induction drugs. CONCLUSION The expression of 15-PGDH is decreased in human multidrug-resistant breast cancer line MCF-7/ADR. 15-PGDH induction drugs could increase chemosensitivity, promote apoptosis and reverse resistance of MCF-7/ADR cell, the mechanism might related to the influence of PGE₂ level by regulated the expression of 15-PGDH and COX-2.     

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??Endogenous neurotoxin 1-methyl-6,7-dihydroxy-1,2,3,4 -tetrahydroisoquinoline (salsolinol,Sals), an endogenous dopamine metabolite, were shown to be toxic to dopaminergic neurons in vitro as well as in vivo, and was known to be involved in the pathogenesis of Parkinson??s disease (PD). Sals is a more realistic model for selective toxicity to nigral dopaminergic neurons, and mimic the natural course of PD that develops slowly, allowing the brain to adapt to progressive damage. Sals lead to neurotoxicity in dopaminergic cells through induction of oxidative stress and apoptotic dopaminergic cell death,which made it as an important tool drugs to study the pathogenesis of PD.     

HPLC法测定朱木匝-15中大黄酚含量

目的:建立HPLC法对朱木匝-15中大黄酚进行含量测定。方法:采用天河C_(18)柱(250mm×4.5mm,5μm)。以乙腈-水-冰乙酸(65:35:1)为流动相,检测波长为437nm,流速为1mL·min~(-1),柱温为40℃。结果:线形范围0.016μg～0.40μg(r=0.9996),平均含量为0.9886mg·g~(-1),RSD为0.44%。结论:本法可作为朱木匝-15中大黄酚含量测定的一种准确、灵敏、可行的方法。     

苦参-15味散灌肠治疗慢性溃疡性结肠炎

<正> 我们用蒙药苦参-15味散灌肠治疗20例慢性溃疡性结肠炎,其疗效明显优于其它中西药治疗。现报告如下。 1 临床资料本组20例,其中男性13例,女性7例;年龄最小者15岁,最大者70岁,平均年龄34岁,多发年龄为30～40岁。病程半年以上3例,1～3年12例,4～6年3例,7～10年2     

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??OBJECTIVE To identify the metabolites of imperatorin in rat urine, feces and bile after oral administration as well as the transformation products of imperatorin after incubation with rat liver microsomes with HPLC-QTrap-MS technology. METHODS The combination use of HPLC-QTrap-MS scanning mode including multiple ion monitoring-information dependent acquisition-enhanced product ion (MRM/MIM-IDA-EPI)mode, precursor scan-enhanced resolution-information dependent acquisition-enhanced product ion (PREC-ER-IDA-EPI)mode and enhanced product ion (EPI) mode were performed for the identification of the metabolites. Based on the simultaneous appearance of [M+H]⁺ and [M+NH₄]⁺ in the spectrum of PREC, the molecular weight could be unambiguously identified. The structures of compounds were then identified by the fragment ions generated from these three modes. RESULTS With the HPLC-QTrap-MS method, 32 metabolites in urine sample, 14 metabolites in faces sample, 6 metabolites in bile sample and 17 transformation products from the rat liver microsomes sample were detected. CONCLUSION Imperatorin is metabolized mainly in liver and excreted through kidney. The metabolic profiles of imperatorin in vivo and in vitro have good correlation.     

清火栀麦片联合重组人白介素-2治疗慢性期布鲁氏菌病患者48例疗效观察

目的:观察清火栀麦片联合重组人白介素-2治疗慢性期布病的临床疗效。方法:90例慢性期布病患者随机分成2组,其中对照组42例,给予利福平0.6 g,1次/d;多西环素0.1 g,2次/d;6周为1个疗程。观察组48例,在对照组的基础上加用重组人白介素-240万IU,皮下注射,3次/周,6周为1个疗程,同时加用清火栀麦片,4粒/次,3次/d,治疗6周后观察治疗效果。结果:观察组患者近期治愈率为37.5%,基本治愈率为33.3%,好转率为20.8%,无效率为8.4%;而对照组患者近期治愈率为21.3%,基本治愈率为16.7%,好转率为19.5%,无效率为42.5%,前者疗效明显好于后者,2组间比较差异有统计学意义(P0.05)。2组患者在治疗前,检测的血清IL-1β、IL-6、5-HT及TNF-α含量比较,差异无统计学意义,而组间治疗后比较,观察组明显低于对照组,P0.05,观察组疗效比对照组好。结论:清火栀麦片联合重组人白介素-2治疗慢性期布病患者具有较好的临床疗效,值得推广。     

首批重组人血白蛋白蛋白质含量RS2国家标准品的研制

目的 研制一种重组人血白蛋白蛋白质含量RS2国家标准品,用于重组人血白蛋白等产品的蛋白质含量测定。方法 选取重组人血白蛋白产品作为原料,经质量浓度9 g·L^-1 NaCl溶液稀释后除菌过滤分装。在8家实验室按经确认和验证的方法进行协作标定并开展稳定性监测。结果 8家实验室采用包括高效液相色谱法(HPLC)、福林酚法(Lowry)、紫外测定法(UV)、2,2′-联喹啉-4,4′-二羧酸法(BCA)等测定本批标准品,蛋白质含量实验室间均值为1 961(Min 1 904,Max 2 063)μg·mL^-1,95%置信区间(1 917,2 016)μg·mL^-1;实验室间标定结果均值标准差52.89,标准误18.70,相对标准偏差(RSD)为2.70%。2~8 ℃持续对该标准品进行稳定性监测19个月,蛋白质含量未发生趋势性变化,建议本品按2~8 ℃保存和运输。结论 重组人血白蛋白蛋白质含量RS2国家标准品符合相关规定,可以发放用于重组人血白蛋白相关的质量控制。     

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??OBJECTIVE To establish the quality control system for the recombinant human GLP-1 analogue fusion protein.METHODS The potency of the fusion protein was determined by luciferase reporter gene assay. The purity was analyzed by non-reduced SDS-PAGE and SEC-HPLC respectively. RP-HPLC was used for the peptide mapping. ELISA was used to analyze the identification of the final products. The molecular mass and peptide mass spectra were analyzed by LC-ESI-MS technique. Other control tests were performed according to the requirements in the Chinese Pharmacopoeia (Volume ??, 2010 edition). RESULTS Control tests were performed on three different lots of bulks and final products of recombinant GLP-1 analog fusion protein by the developed methods. The results showed that all the indexes met the requirements in the Guideline for Quality Control of Recombinant DNA Products for Human Use and Chinese Pharmacopoeia (Volume ??, 2010 edition). The molecular weight of the recombinant human GLP-1 analogue fusion protein was 71 361.0, which was in conformity with the theoretical value. CONCLUSION The developed methods and standard may assure the safety, effectiveness, and controllability of the recombinant human GLP-1 analogue fusion protein, which might be used for the routine quality control of products of the same kind.     

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??OBJECTIVE To determine glycoprotein content in recombinant human albumin from different expression systems with different methods. METHODS Recombinant human albumin samples from Saccharomyces cerevisiae expression system, Pichia pastoris expression system, Oryza sativa expression system as well as plasma derived human albumin were investigated by phenol sulfuric acid method, HPLC peak area method and ConA combining elution HPLC method. RESULTS For 10 batches of samples expressed in pichia pastoris expression system, the total contents of mannose were 2.7 mg·g(Pro)^-1 (A manufacturer, n=4) and 1.7 mg·g(Pro)^-1 (B manufacturer, n=6), respectively. The HPLC peak area percentages of ConA binding protein in recombinant human albumin from Pichia pastoris expression system were the highest, which showed 2.65% (A manufacturer) and 0.71% (B manufacturer)respectively, the peak area percentage of ConA binding protein in Oryza sativa expression system was 0.05% (E manufacturer, n=3), and the ConA binding protein peak area of plasma derived human albumin was 0.01% (W manufacturer). The RESULTS of ConA binding and elution analysis with HPLC method for Quantitative determination of ConA binding protein showed that the ConA binding protein contents in the samples from pichia pastoris expression system were much higher: 27.58 mg·g(Pro)^-1 (A manufacturer), 21.48 mg·g(Pro)^-1(B manufacturer), 32.02 mg·g(Pro)^-1(C manufacturer); the ConA binding protein content in the sample from Saccharomyces cerevisiae expression system was lower, 2.29 mg·g(Pro)^-1(D manufacturer); the ConA binding protein content in the samples from oryza sativa expression system was the lowest, 1.27 mg·g(Pro)^-1 (E manufacturer); the plasma derived human albumin ConA binding protein content was 31.16 mg·g(Pro)^-1 (S manufacturer). CONCLUSION In terms of the RESULTS of the samples and METHODS involved in this study,there were glycosylated or glycol forms of protein in all recombinant human albumin samples from different expression systems; the glycosylated protein content in samples of Pichia pastoris expression system is higher than Saccharomyces cerevisiae expression system, while the glycoformed protein in samples of Oryza sativa expression system is the lowest. Plasma derived human albumin also contains glycoprotein or glycosylated protein.     

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??OBJECTIVE To introduce the procedure of developing reference standards of fluconazole impurities using fluconazole impurity H as an example and reveal a special problem for establishment of national reference standards. METHODS Firstly, the structure of fluconazole impurity H was validated by infrared, mass spectrum and nuclear magnetic resonance method. Secondly, its purity was determined using the related substances test of fluconazole in European Pharmacopoeia 8.0 (EP8.0) and Chinese Pharmacopeia (2010 version, Volume 2, ChP2010). Then the major impurities determined by the above two HPLC systems were analyzed by LC-MS method. Finally, the content of fluconazole impurity H, its water content and inorganic impurities were determined by quantitative nuclear magnetic resonance (qNMR) method, Karl Fischer titrimetry method and residue on ignition method respectively. RESULTS The structure of fluconazole impurity H was identical with that in EP 8.0. The contents of water and inorganic impurities were 0.05% and 0.04%, respectively. It was found that fluconazole impurity H would be partially degraded into fluconazole impurity G in water solution, which resulted in the inaccuracy of the related substances test. The content of impurity H was 99.5% by qNMR method. CONCLUSION Due to the structural characteristics of fluconazole impurity H, the mass balance method, which is the routine method for determination of the content of reference standards, is not suitable for fluconazole impurity H. In the circumstances, qNMR method can be used as a complementary method for the content determination of reference standards.     

In Vitro Evaluation of Bacopa monniera Extract and Individual Constituents on Human Recombinant Monoamine Oxidase Enzymes

Bacopa monniera is a traditional Ayurvedic medicinal plant that has been used worldwide for its nootropic action. Chemically standardized extract of B. monniera is now available as over the counter herbal remedy to enhance memory in children and adults. Considering the nootropic action of B. monniera, we evaluated the effect of clinically available B. monniera extract and six of B. monniera constituents (bacoside A3, bacopaside I, bacopaside II, bacosaponin C, bacosine, and bacoside A mixture) on recombinant human monoamine oxidase (MAO) enzymes. The effect of B. monniera extract and individual constituents on human recombinant MAO‐A and MAO‐B enzymes was evaluated using MAO‐Glo^TM assay kit (Promega Corporation, USA), following the instruction manual. IC₅₀ and mode of inhibition were measured for MAO enzymes. Bacopaside I and bacoside A mixture inhibited the MAO‐A and MAO‐B enzymes. Bacopaside I exhibited mixed mode of inhibition with IC₅₀ and Ki values of 17.08 ± 1.64 and 42.5 ± 3.53 µg/mL, respectively, for MAO‐A enzyme. Bacopaside I is the major constituent of B. monniera, which inhibited the MAO‐A enzyme selectively. Copyright © 2014 John Wiley & Sons, Ltd.     

借鉴国际标准 提高管理水平——关于医院管理质量认证标准的比较研究

文章通过对ISO9001标准、英国保柏“医院管理以及医疗服务质量”评估体系标准、JCI国际医院认证标准的特点进行简要比较，旨在探讨适合于中国医院管理、提高医疗服务质量、保障患者权益方面、实施国标化战略的思路与方法。     

五倍子鞣酸对照品的制备及质量标准研究

目的:制备五倍子鞣酸精制品（对照品）及制定质量标准。方法:应用大孔高分子吸附树脂精制五倍子鞣酸;采用HPLC及LC/MS图谱研究五倍子鞣酸精制品的质量。结果:五倍子鞣酸精制品含量≥98%,没食子酸含量<0.2%,具稳定的HPLC特征图谱。精制收得率64%。并制定相应的质量标准。结论:以大孔高分子吸附树脂精制五倍子鞣酸可作为鞣酸含量测定法（中国药典2000年版一部附录XB）的替代法的对照品应用。     

五倍子鞣酸对照品的制备及质量标准研究

目的:制备五倍子鞣酸精制品(对照品)及制定质量标准。方法:应用大孔高分子吸附树脂精制五倍子鞣酸;采用HPLC及LC/MS图谱研究五倍子鞣酸精制品的质量。结果:五倍子鞣酸精制品含量≥98%,没食子酸含量<0.2%,具稳定的HPLC特征图谱。精制收得率64%。并制定相应的质量标准。结论:以大孔高分子吸附树脂精制五倍子鞣酸可作为鞣酸含量测定法(中国药典2000年版一部附录XB)的替代法的对照品应用。     

合欢花饮片标准汤剂质量标准评价的建立

目的:制备合欢花饮片标准汤剂,测定合欢花标准汤剂中槲皮苷的含量,并建立指纹图谱,以进行质量研究。方法:依照标准汤剂的制备要求,制备15批不同产地的合欢花饮片标准汤剂,以槲皮苷作为定量检测指标,计算转移率与出膏率,并建立其UPLC特征图谱分析方法。结果:通过对15批合欢花标准汤剂进行测定,槲皮苷转移率为72.33%～41.51%,出膏率为26.6%～23.4%;并用中药色谱指纹图谱相似度评价系统软件进行特征图谱分析,标定了其中5个共有峰,对15批合欢花饮片标准汤剂分别进行了相似度评价,其相似度均大于0.90。结论:合欢花饮片标准汤剂制备规范,测定方法精密度、稳定性和重复性良好,可为合欢花配方颗粒的质量控制提供参考。