瑶药扁担藤化学成分的研究

 目的 研究我国瑶族植物药扁担藤[Tetrastigma planicaule(Hook.) Gagnep.]的化学成分。方法 采用色谱技术进行分离,根据化合物的理化性质和光谱数据鉴定其结构。结果 从扁担藤茎的体积分数95%乙醇提取物中分离得到8个化合物,分别鉴定为stigmast-4-en-6β-ol-3-one(1),7α-hydroxysitosterol(2),古柯二醇(3),水杨酸(4),香草酸(5),丁香酸(6),原儿茶酸(7),glycerol-2-(3-methoxy-4-hydroxybenzoic acid) ether(8)。结论 所有化合物均为首次从该植物中分离得到,其中化合物1~3,5~8为首次从该属植物中分得。化合物8为首次从天然产物中分离得到。     

骆驼刺的化学成分研究

 目的研究骆驼刺[Alhagi pseudalhagi(M.B.)]的化学成分。方法采用反复硅胶柱色谱法、SephadexLH-20柱色谱法等进行分离纯化,并通过化合物的理化性质和波谱数据进行结构鉴定。结果从骆驼刺体积分数70%乙醇提取物中分离得到10个化合物,其中4个异黄酮类化合物:芒柄花素(1),3′,7-二羟基-4′-甲氧基异黄酮(2),3′,7-二羟基-4′,8-二甲氧基异黄酮(3),红车轴草素(4);2个黄酮类化合物:柽柳素(5),异槲皮苷(6);2个酚酸类化合物:水杨酸(7),香草酸(8);β-谷甾醇(9),胡萝卜苷(10)。结论化合物1～4,7,8均为首次从该属植物中分离得到。     

刮筋板的化学成分和抗肿瘤活性研究

 目的 研究刮筋板 Excoecaria acerifclia F. Didr. 的化学成分和抗肿瘤活性。 方法 采用硅胶柱色谱, Sephedex LH - 20 分离化学成分 ; 根据波谱数据和理化性质确定化学结构;四甲基偶氮唑蓝（ MTT ） 法测定化合物对人肝癌细胞系 ( human hepatocarcinoma cell line ) HepG2 的 体外抑制作用,用半数抑制浓度 (IC₅₀) 评价其抗肿瘤活性。 结果 分离鉴定 6 个化合物,分别为：秦皮苷（ 1 ）、异落叶松脂醇 9-<>O -β-D- 葡萄糖苷（ 2 ）、 (-)-5 ′ -methoxyisolariciresinol 3a- O-β-<>D-glucopyranoside （ 3 ）、没食子酸（ 4 ）、 isostrictiniin （ 5 ）、 3-<>O- 阿魏酰基奎尼酸甲酯（ 6 ）;化合物 1 , 2 , 4 , 6 对体外培养肿瘤细胞的 IC₅₀ 分别为 14.71 , 26.52 , 34.91 , 65.42 μmol·L^-1 ; 在测定浓度范围内剂量依赖关系良好。 结论 所有化合物均为首次从该植物中得到;首次研究发现化合物 1 对 HepG 2 具有一定的抗肿瘤活性。     

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??OBJECTIVE To study the chemical constituents from the leaves of Jiangxi genuine medicinal material Chimonanthus nitens Oliv. METHODS The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20, ODS column chromatography, semi-preparative HPLC, and so on. Their structures were elucidated on the basis of physiochemical properties and spectral data. RESULTS Ten compounds were isolated and elucidated as uracil (1), 6,7-dimethoxycoumarin (2), 6,7,8-trimethoxycoumarin (3), p-hydroxybenzoic acid ethyl ester (4), 1??1 mixture of two diastereomers identical with (3RS,6RS)-2, 6-dimethyl-octa-1,7-dien-3,6-diol (5 and 6), kaempferol (7), isofraxidin (8), scopoletin (9), and loliolide (10). CONCLUSION Compounds 1, 3-6 and 10 are isolated from this plant for the first time and compounds 1, 4-6 are isolated from this genus for the first time.     

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??OBJECTIVE To study the anti-inflammatory active constituents of Mangifera indica L. seed kernel. METHODS The compounds were isolated by silica gel column chromatography, Sephadex LH-20 column chromatography and ODS column chromatography. Their structures were elucidated by spectral analyses and physicochemical properties. The anti-inflammatory activities of selected isolated compounds were evaluated as inhibitory activities against lipopolysaccharide(LPS)-induced nitric oxide(NO) production in RAW264.7 cell lines by Griess reaction. RESULTS Eight compounds were obtained from n-butanol fraction of Mangifera indica L. seed kernel,whose structures were elucidated as 4-O-ethylgallic acid(1), gallic acid(2), citric acid(3), 1,2,3,4,6-penta-O-galloylglucose(4),1,3,6-tri-O-galloylglucose(5), hyperoside(6), quercetin-3-O-rhamnopyranoside(7), and mangiferin(8). Compounds 1, 2 and 4-8 exhibited potent inhibitory effect on NO production in LPS-induced macrophages, especially compounds 6 and 8 showed the best inhibitory activity with IC₅₀ values of 16.5 and 19.5 ??mol??L^-1, respectively. CONCLUSION Compounds 1 and 3-8 were obtained from Mangifera indica L. seed kernel for the first time. Compounds 1, 2 and 4-8 are the anti-inflammatory active constituents of Mangifera indica L. seed kernel. Compounds 6 and 8 are the most potent anti-inflammatory active constituents.     

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??OBJECTIVE To study the chemical constituents of Magnolia biondii.Pamp. METHODS The compounds were isolated and purified by Diaion HP-20, Toyopearl HW-40, silica gel column chromatography and semi-preparative HPLC and so on. The structures were elucidated on the basis of spectral data and physiochemical properties. RESULTS Fourteen compounds were isolated and identified as 4-O-??-D-glucopyranosylvanillic acid(1), tachinoside(2), methyl 4-hydroxy-3-methoxybenzoate(3), caffeic acid(4), 3,4,5-trimethoxyphenyl-??-D-glucopyranoside(5), benzyl-O-??-D-glucopyranoside(6), benzyl-O-??-D-galactopyranoside(7), syringin(8), vanillic acid glucosyl ester(9), vanillic acid(10), 1??-(3,4-dihydroxycinnamoyl)cyclopentane-2??,3??-diol(11), scopolin(12),7-methoxycoumarin-6-O-??-D-glucopyranoside(13), and scopoletin(14). CONCLUSION Compounds 1-9 and 11-13 are isolated from this plant for the first time.     

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??OBJECTIVE To study the chemical constituents from the roots of Rubus crataegifolius Bge. METHODS The compounds were isolated and purified by column chromatography and their structures were elucidated by spectroscopic METHODS. RESULTS Ten compounds were isolated and identified as(+)-catechin(1),3,4,5-trihydroxybenzoic acid(2),oleanolic acid(3),ursolic acid(4),ducheside B(5),dehydrodicatechin A(6),daucosterol(7),p-hydroxybenzoic acid(8),??-sitosterol linoleate(9), and euscaphic acid(10). CONCLUSION Compounds 1-10 are obtained from this plant for the first time.     

绵毛酸模叶蓼中2个新的蔗糖桂皮酸酯类化合物

采用硅胶柱色谱、ODS柱色谱和半制备高效液相等多种色谱分离技术,从绵毛酸模叶蓼的乙醇提取物中分离得到11个化合物,分别为7个蔗糖桂皮酸酯类化合物,3个苯丙素类化合物和1个内酯类化合物.根据一维、二维核磁共振及高分辨质谱等波谱数据鉴定了该系列化合物的结构,分别鉴定为(1,3-O-di-p-coumaroyl)-β-D-f...     

青龙衣酚酸类化学成分研究

目的:研究核桃楸(Juglans mandshurica Maxim)未成熟外果皮中酚酸类成分。方法:青龙衣95%乙醇提取物的乙酸乙酯部位采用反复硅胶柱色谱、Sephadex LH-20、ODS柱色谱和半制备HPLC等进行分离纯化,通过HR-ESI-MS和NMR波谱数据鉴定化合物结构。结果:从中分离得到15个化合物,分别鉴定为对苯二酚(1)、2,5-二甲基间苯二酚(2)、咖啡酸(3)、香草酸(4)、丁香酸(5)、原儿茶酸(6)、对羟基苯甲酸(7)、2-羟基-4-甲氧基-3,6-二甲基苯甲酸(8)、3′-O-(E-4-coumaroyl)-quinic acid(9)、5′-O-(E-4-coumaroyl)-quinic acid(10)、3,3′-二甲氧基鞣花酸(11)、阿魏酰乳酸二甲酯(12)、没食子酸乙酯(13)、(S)-3-羟基-1,5-二苯基-1-戊酮(14)、Z-P-coumaryl-hexacosanoate(15)。结论:化合物8~15为首次从该植物中分离得到。     

光叶珙桐化学成分的研究

目的:研究光叶珙桐 Davidia involucrata var.vilmoriniana 枝叶的化学成分。方法:采用薄层色谱,硅胶柱色谱,Sephadex LH-20凝胶柱色谱等方法进行分离纯化,使用波谱分析技术和理化常数对照等方法对得到的化合物进行结构鉴定。结果:从光叶珙桐的醋酸乙酯部位分离得到7个化合物,分别为没食子酸(3,4,5-trihydroxy-benzoic acid1),鞣花酸(ellagic acid2),顺丁烯二酸(maleic acid3),槲皮素(quercetin4),3-O-β-D-吡喃半乳糖基-槲皮素苷(quercetin-3-O-β-D-galactoside5),2α,3α,19α,23-tetrahydroxyurs-12-en-28-oic acid-28-O-β-D-glucopyranosylester(6),短叶苏木酚酸乙酯(ethyl brevifolin carbosylate7)。结论:化合物1~7均为首次从该植物中分离得到。