乙肝病毒X蛋白在肝癌转移中的作用

乙肝病毒X蛋白(HBx)是乙肝病毒感染相关性肝癌的重要致病因子,与原发性肝细胞癌(HCC)的发生与发展密切相关.研究表明,HBx也参与血管生成、HGW/c-met信号通路、基质金属蛋白酶(MMP)等相关因素而促进肝癌转移.加强HBx参与肝癌侵袭转移分子机制的研究,有助于完善HCC侵袭转移机制,以进一步提高肝癌的疗效.     

乙肝病毒X蛋白在肝癌转移中的作用

乙肝病毒X蛋白(HBx)是乙肝病毒感染相关性肝癌的重要致病因子,与原发性肝细胞癌(HCC)的发生与发展密切相关.研究表明,HBx也参与血管生成、HGW/c-met信号通路、基质金属蛋白酶(MMP)等相关因素而促进肝癌转移.加强HBx参与肝癌侵袭转移分子机制的研究,有助于完善HCC侵袭转移机制,以进一步提高肝癌的疗效.     

乙肝病毒X蛋白在肝癌转移中的作用

乙肝病毒X蛋白(HBx)是乙肝病毒感染相关性肝癌的重要致病因子,与原发性肝细胞癌(HCC)的发生与发展密切相关.研究表明,HBx也参与血管生成、HGW/c-met信号通路、基质金属蛋白酶(MMP)等相关因素而促进肝癌转移.加强HBx参与肝癌侵袭转移分子机制的研究,有助于完善HCC侵袭转移机制,以进一步提高肝癌的疗效.     

乙肝病毒X蛋白在肝癌转移中的作用

乙肝病毒X蛋白(HBx)是乙肝病毒感染相关性肝癌的重要致病因子,与原发性肝细胞癌(HCC)的发生与发展密切相关.研究表明,HBx也参与血管生成、HGW/c-met信号通路、基质金属蛋白酶(MMP)等相关因素而促进肝癌转移.加强HBx参与肝癌侵袭转移分子机制的研究,有助于完善HCC侵袭转移机制,以进一步提高肝癌的疗效.     

乙肝病毒X蛋白在肝癌转移中的作用

乙肝病毒X蛋白(HBx)是乙肝病毒感染相关性肝癌的重要致病因子,与原发性肝细胞癌(HCC)的发生与发展密切相关.研究表明,HBx也参与血管生成、HGW/c-met信号通路、基质金属蛋白酶(MMP)等相关因素而促进肝癌转移.加强HBx参与肝癌侵袭转移分子机制的研究,有助于完善HCC侵袭转移机制,以进一步提高肝癌的疗效.     

乙肝病毒X蛋白在肝癌转移中的作用

乙肝病毒X蛋白(HBx)是乙肝病毒感染相关性肝癌的重要致病因子,与原发性肝细胞癌(HCC)的发生与发展密切相关.研究表明,HBx也参与血管生成、HGW/c-met信号通路、基质金属蛋白酶(MMP)等相关因素而促进肝癌转移.加强HBx参与肝癌侵袭转移分子机制的研究,有助于完善HCC侵袭转移机制,以进一步提高肝癌的疗效.     

乙肝病毒X蛋白在肝癌转移中的作用

乙肝病毒X蛋白(HBx)是乙肝病毒感染相关性肝癌的重要致病因子,与原发性肝细胞癌(HCC)的发生与发展密切相关.研究表明,HBx也参与血管生成、HGW/c-met信号通路、基质金属蛋白酶(MMP)等相关因素而促进肝癌转移.加强HBx参与肝癌侵袭转移分子机制的研究,有助于完善HCC侵袭转移机制,以进一步提高肝癌的疗效.     

乙肝病毒X蛋白在肝癌转移中的作用

乙肝病毒X蛋白(HBx)是乙肝病毒感染相关性肝癌的重要致病因子,与原发性肝细胞癌(HCC)的发生与发展密切相关.研究表明,HBx也参与血管生成、HGW/c-met信号通路、基质金属蛋白酶(MMP)等相关因素而促进肝癌转移.加强HBx参与肝癌侵袭转移分子机制的研究,有助于完善HCC侵袭转移机制,以进一步提高肝癌的疗效.     

乙肝病毒X蛋白在肝癌转移中的作用

乙肝病毒X蛋白(HBx)是乙肝病毒感染相关性肝癌的重要致病因子,与原发性肝细胞癌(HCC)的发生与发展密切相关.研究表明,HBx也参与血管生成、HGW/c-met信号通路、基质金属蛋白酶(MMP)等相关因素而促进肝癌转移.加强HBx参与肝癌侵袭转移分子机制的研究,有助于完善HCC侵袭转移机制,以进一步提高肝癌的疗效.     

乙肝病毒X蛋白在肝癌转移中的作用

乙肝病毒X蛋白(HBx)是乙肝病毒感染相关性肝癌的重要致病因子,与原发性肝细胞癌(HCC)的发生与发展密切相关.研究表明,HBx也参与血管生成、HGW/c-met信号通路、基质金属蛋白酶(MMP)等相关因素而促进肝癌转移.加强HBx参与肝癌侵袭转移分子机制的研究,有助于完善HCC侵袭转移机制,以进一步提高肝癌的疗效.     

乙型肝炎病毒X蛋白在肝细胞癌微环境中的作用及其机制

乙型肝炎病毒（HBV）慢性感染是诱发肝细胞癌（HCC）的重要危险因素，而炎症的持续存在可形成一种适宜肿瘤存活 的微环境（TME），其X基因编码并表达的乙型肝炎病毒X蛋白（HbX）作为一个多功能调节蛋白，它通过与TME中的肿瘤细胞及 周围基质成分相互作用，促进HCC的发生和发展。本文通过对近期文献进行回顾总结，着重分析了HbX在HCC-TME中引发 HCC侵袭和转移的作用机制，包括参与癌细胞增殖与凋亡、抑制机体免疫、促进肝细胞自噬、诱导细胞外基质重塑以及调控干细 胞特性等方面，以期为临床HBV相关HCC提供新的治疗思路。     

HBV X蛋白和HCV核心蛋白在肝癌发生中的作用

原发性肝癌（HCC）是我国最常见的恶性肿瘤之一,乙型肝炎病毒（HBV）X蛋白和丙型肝炎病毒（HCV）核心蛋白可通过影响肝细胞内信号传导及细胞凋亡等机制,调节某些基因的转录及表达,参与HCC的发生,两者可能存在协同致癌作用。     

乙肝病毒X蛋白对肝细胞生物钟基因的影响及其意义

Objective:The aim of this study was to investigate the influence of hepatitis B virus X protein (HBx) on the clock genes in LO2 cells and its significance.Methods:A cell line LO2-HBx,Stably transfected with HBx gene,was established.The levels of mRNA and protein expression of CLOCK and BMAL1 were detected by real-time PCR and western blot.Results:The expression of CLOCK mRNA and protein were increased in cell line LO2-HBx (P<0.05),while the expression of BMAL1 mRNA and protein were decreased in cell line LO...     

Hepatitis B virus X protein in liver tumor microenvironment

Encoded by the hepatitis B virus, hepatitis B virus X protein (HBx) is a multifunctional, potentially oncogenic protein that acts primarily during the progression from chronic hepatitis B to cirrhosis and hepatocellular carcinoma (HCC). In recent decades, it has been established that chronic inflammation generates a tumor-supporting microenvironment. HCC is a typical chronic inflammation-related cancer, and inflammation is the main risk factor for HCC progression. The viral transactivator HBx plays a pivotal role in the initiation and maintenance of hepatic inflammatory processes through interactions with components of the tumor microenvironment including tumor cells and the surrounding peritumoral stroma. The complex interactions between HBx and this microenvironment are thought to regulate tumor growth, progression, invasion, metastasis, and angiogenesis. In this review, we have summarized the current evidence evaluating the function of HBx and its contribution to the inflammatory liver tumor microenvironment.     

Resveratrol helps recovery from fatty liver and protects against hepatocellular carcinoma induced by hepatitis B virus X protein in a mouse model

Resveratrol is a natural polyphenol that has beneficial effects across species and various disease models. Here, we investigate whether resveratrol is effective against hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC) using HBV X protein (HBx) transgenic mice. We found that resveratrol (30 mg/kg/d) has a therapeutic effect on HBx-induced fatty liver and the early stages of liver damage. Resveratrol decreased intracellular reactive oxygen species and transiently stimulated hepatocyte proliferation. Interestingly, resveratrol inhibited LXRα and downregulated the expression of the lipogenic genes, Srebp1-c and PPARγ. The decrease in Srebp1-c seems to further downregulate the expression of its target genes, Acc and Fas. In addition, resveratrol stimulated the activity of Ampk and SirT1. Thus, resveratrol has a pleiotropic effect on HBx transgenic mice in terms of the downregulation of lipogenesis, the promotion of transient liver regeneration, and the stimulation of antioxidant activity. Furthermore, at the later precancerous stages, resveratrol delayed HBx-mediated hepatocarcinogenesis and reduced HCC incidence from 80% to 15%, a 5.3-fold reduction. Resveratrol should be considered as a potential chemopreventive agent for HBV-associated HCC.     

The BH3-only protein BimL overrides Bcl-2-mediated apoptosis resistance in melanoma cells

Melanoma cells are characterized by apoptosis deficiency coinciding with reduced expression of the proapoptotic Bcl-2 protein Bim. An adenoviral vector was constructed with the Bim_L cDNA controlled by an inducible promoter. Highly efficient apoptosis induction and abrogated cell proliferation was seen in melanoma cells upon Bim_L overexpression. Loss of mitochondrial membrane potential, release of mitochondrial apoptogenic factors and caspase-9 processing indicated the activation of mitochondrial apoptosis pathways. Bim_L activated both Bax and Bak, as shown by siRNA knockdown and activation-specific antibodies. Of note, Bim_L overrode the apoptosis blockade by Bcl-2 overexpression or by Bax/Bak single knockdown. The high efficacy correlated to Bim_L interaction with all antiapoptotic Bcl-2 family members in melanoma cells, shown by co-immunoprecipitation analyses for Bcl-2, Bcl-x_L, Mcl-1 and Bcl-w. Thus, Bim_L reveals an outstanding proapoptotic potential in melanoma cells, and strategies for its re-expression appear of interest. These have been reported for B-Raf inhibitors, and their efficacy may be partly attributed to Bim_L.     

乙型肝炎病毒X蛋白与肝癌研究进展

X蛋白(HBx)被认为是乙型肝炎病毒(HBV)致原发性肝癌(HCC)的重要物质,具体机制不明,己成为研究的热点之一。近年来对HBx致肝癌机制的研究显示,HBx是一种多功能的病毒蛋白,有转录调控作用,对肝细胞内许多癌基因和/或抑癌基因的表达有直接或间接影响,可影响细胞的生存、增殖和凋亡,调节转录因子活性,促进细胞的侵袭和转移等,在肝癌形成中起重要作用。     

乙肝病毒X蛋白结合蛋白(HBXIP)在肝癌中的表达及定位

目的:研究乙肝病毒X蛋白结合蛋白(HBXIP)在人肝癌细胞中的表达和定位.方法:蛋白印迹法检测HBXIP在人肝癌细胞系中蛋白和基因的表达,激光共聚焦扫描显微镜检测内源HBXIP在BEL7402、HepG2肝癌细胞中的定位.结果:HBXIP在人肝肿瘤细胞系中蛋白和基因表达水平增高,激光共聚焦扫描显微镜观察发现HBXIP主要定位于BEL7402、HepG2细胞核中.结论:HBXIP在人肝癌细胞系中高表达,主要定位于肝癌细胞核内,可能是人肝癌重要的调节因子.     

乙肝病毒X蛋白促进肝细胞癌侵袭转移相关分子的作用机制

乙肝病毒X蛋白(HBx)是乙型肝炎病毒编码的病毒蛋白,在介导肝细胞癌(HCC)转移中起重要作用.新近研究发现,HBx可通过介导肿瘤转移相关分子,如基质金属蛋白酶(MMP)、血管内皮生长因子(VEGF)及尿激酶纤溶酶原激活因子(uPA)等表达,进而活化转移相关信号传导通路,促进HCC转移.