腹腔镜下子宫肌瘤剔除术30例

目的探讨腹腔镜下子宫肌瘤剔除术的治疗效果及其影响因素。方法腹腔镜下对30例子宫肌瘤患者行子宫肌瘤剔除术,其中单发23例,多发7例。单发肌瘤中肌壁间肌瘤15例,浆膜下肌瘤6例,阔韧带肌瘤2例。结果30例患者均手术成功,术中出血（115±33）ml,手术时间（110±46）min,术后肛门排气时间（1．7±0．4）d,术后病率为0,术后住院天数为（5．2±1．7）d,无严重并发症。结论腹腔镜下子宫肌瘤剔除术是一种安全、可靠、创伤小的手术方法,术中出血及手术时间主要与肌瘤的生长部位和大小有关。     

腹腔镜下子宫肌瘤剔除术180例临床分析

目的探讨腹腔镜下子宫肌瘤剔除术的手术方法、适应证、术后疗效及其影响因素。方法回顾性分析腹腔镜下180例子宫肌瘤剔除术，其中单发肌瘤102例，多发肌瘤78例；单发肌瘤包括肌壁间肌瘤62例，浆膜下肌瘤36例，阔韧带肌瘤4例。结果除1例阔韧带肌瘤靠近子宫动脉中转开腹外，其余手术均获成功，术中出血（62±38）ml；手术时间（112±35）min；术后（3．8±2．8）d肛门排气；术后住院天数（5．8±0．8）d；无严重并发症。术中出血量和手术时间主要与肌瘤生长部位及其大小有关。结论腹腔镜下子宫肌瘤剔除术是目前较为理想、安全有效的保留子宫的手术方法。     

腹腔镜子宫肌瘤剔除术76例临床分析

目的：探讨腹腔镜下子宫肌瘤剔除术的临床应用价值及手术操作技巧。方法：对76例子宫肌瘤患者行腹腔镜下子宫肌瘤剔除术,明确临床应用价值,总结手术操作技巧。结果：76例手术全部成功,其中无蒂浆膜下肌瘤及壁间肌瘤65例,有蒂浆膜下肌瘤5例,阔韧带肌瘤4例,宫颈肌瘤2例。肌瘤直径3.5～10cm。平均手术时间（87±35）min,术中出血量（89±23）ml,术后肛门排气时间（22±6）h,术后体温最高38℃,2d后恢复正常,术后住院天数（5.2±1.5）d。结论：腹腔镜下子宫肌瘤剔除术是一种安全、有效的保留器官的手术方法,具有损伤小、出血少、恢复快等优点,术前严格掌握手术指征及术中良好的手术操作技巧是手术成功的关键。     

较困难的腹腔镜子宫肌瘤剔除术32例临床分析

目的 探讨较困难的腹腔镜子宫肌瘤剔除术（直径≥7 cm的较大肌瘤、多发子宫肌瘤致子宫如孕〉3个月、深层肌壁间肌瘤及特殊位置肌瘤）的可行性、安全性及手术技巧.方法 回顾性分析32例较困难的腹腔镜子宫肌瘤剔除术患者的临床资料,观察手术时间、术中出血量、术后并发症.结果 除2例由于肌瘤过多（6-15个）、过大（最大直径13 cm）,术中出血500-700 ml,辅以腹部小切口（5 cm）完成手术,余均在腹腔镜下完成.平均手术时间（112.7±35.2）min,术中平均出血量（145±60）ml,无严重术中、术后并发症.结论 较困难的腹腔镜子宫肌瘤剔除术安全可行,提高腹腔镜手术技巧是手术成功的关键.     

腹腔镜下子宫肌瘤剔除术的临床分析

目的:探讨腹腔镜下子宫肌瘤剔除术的手术方式、适应证及疗效。方法:对60例腹腔镜下子宫肌瘤剔除术的操作及疗效等方面进行分析,包括手术方法及疗效。结果:腹腔镜下子宫肌瘤剔除术成功59例,1例因盆腔广泛粘连腹腔镜下手术治疗操作困难而转开腹。术中出血50~150ml,平均100ml;手术时间35~180min,平均80min;术后住院时间4~6d,平均5d。术后无并发症及副损伤发生。结论:腹腔镜下子宫肌瘤剔除术创伤小、恢复快、术后病率低、住院时间短、腹部切口美观、盆腔粘连少、不影响妊娠,是一种保留子宫的理想术式,是具有良好应用前景的微创外科手术。     

腹腔镜下子宫肌瘤剔除术80例报告

目的 探讨腹腔镜下子宫肌瘤剔除术的手术要点及临床应用价值.方法 对80例临床拟诊为子宫肌瘤的患者行腹腔镜下子宫肌瘤剔除术.结果 80例手术均在腹腔镜下完成.术中见单发子宫肌瘤52例,多发性子宫肌瘤26例.子宫腺肌瘤2例;手术时闻平均100min,术中出血量平均90ml,术后住院天数平均5d,无手术并发症.结论 腹腔镜下子宫肌瘤剔除术是一种安全、可靠的手术方式,对年轻、要求保留子宫的患者是一种较为理想的微创手术治疗方法之一.     

腹腔镜子宫肌瘤剔除术30例临床分析

目的探讨腹腔镜子宫肌瘤剔除术不同部位的肌瘤的处理特点。方法对30例子宫肌瘤患者行腹腔镜子宫肌瘤剔除术。结果30例腹腔镜手术全部完成，其中1例病理报告为高分化平滑肌瘤，立即追加开腹行全宫切除术，单发性肌瘤24例，其中浆膜下肌瘤4例，肌壁间肌瘤14例，阔韧带肌瘤4例，宫颈肌瘤2例。多发性肌瘤6例，肌瘤数2～5个。肌瘤的直径1～13cm，术中出血10～100ml，术后住院平均3．5d。结论腹腔镜下子宫肌瘤剔除在选择好病例的基础上，对浆膜下肌壁间肌瘤，阔韧带肌瘤，宫颈肌瘤分别采用电切，分离，缝扎等术式，安全可靠，术后恢复快，尤其对保留子宫未生育妇女尤为重要。     

腹腔镜子宫肌瘤剔除术208例分析

目的探讨腹腔镜下子宫肌瘤剔除术的手术方法、技巧以及临床应用。方法对我院2003年8月-2008年12月208例腹腔镜子宫肌瘤剔除术患者的临床资料进行回顾性分析。结果208例患者共剔除肌瘤253个;腹腔镜下完成204例,4例中转开腹：平均出血量（82±88）ml;平均住院时间3．8d。结论腹腔镜下子宫肌瘤剔除术具有创伤小、术中出血少、术后恢复快及住院时间短的优势,子宫肌瘤的腹腔镜剔除手术无疑是一种保留器官的理想术式,随着腹腔镜手术技巧的提高和器械的更新改进,腹腔镜下子宫肌瘤剔除术将有更广阔的临床应用前景。     

经阴道子宫肌瘤剔除术36例临床分析

目的探讨经阴道子宫肌瘤剔除术的手术适应证及手术技巧。方法2006年12月-2007年12月，对36例子宫体部肌瘤（直径1cm-7cm）行经阴道子宫肌瘤剔除术，经阴道前或后穹隆横切口或阴道前后穹隆切口进盆腔，在近肌瘤部位的子宫壁上缝针，用丝线牵拉，使肌瘤暴露于阴道切口，常规剔除肌瘤，可吸收线分别缝合子宫及阴道切口。结果36例手术均成功，手术时间45min-150min，平均75min，术中出血量60-200ml，术后住院3d-5d，平均3．5d。1个月后B超检查未发现肌瘤残留，28例术后随诊3个月N12个月，平均6个月，无肌瘤复发。4例有生育要求患者妊娠。结论经阴道子宫肌瘤剔除手术安全、可靠，适用于子宫体部浆膜下或肌壁间肌瘤．术前充分评估，正确选择切口，对手术的安全性和手术成功较为重要。子宫翻出是手术难点，应用丝线缝拉子宫，使肌瘤暴露于阴道切口，剔除后子宫翻转容易，降低了手术难度。正确缝合瘤腔，可防止血肿形成和术后复发。     

经阴道子宫肌瘤切除术20例临床分析

目的探讨经阴道子宫肌瘤切除术的效果和安全性。方法探讨经阴道子宫肌瘤切除术患者的手术适应证、并发症、术后恢复情况和治疗效果。结果20例手术均获得成功，手术时间30—150min，平均69min，术中出血量60～200ml，平均92ml，术后住院2～6d，平均3．5d。1个月后B超检查未发现肌瘤残留。20例随诊6～30个月，平均18个月，肌瘤无复发。结论经阴道子宫肌瘤剔除术具有创伤小、恢复快等优点，适用于宫体部肌壁间及浆膜下肌瘤，阴道前穹窿纵切口的选择，降低了手术难度。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.