瘦素抵抗与载脂蛋白E基因多态性对儿童血脂的影响

目的 探讨瘦素(leptin)及瘦素抵抗与载脂蛋白(apoprotein,Apo)E基因多态性对肥胖儿童脂质代谢的影响,为肥胖所致相关疾病提供早期预防措施.方法 选择2009年7月至2010年10月在本院门诊就诊、住院确诊及体检时发现的儿童肥胖症患儿99例(研究组)和健康儿童76例(对照组),年龄均为6～14岁.对研究组儿童,再根据瘦素水平分为瘦素抵抗型组 (>9.21 ng/mL,n=71)和瘦素敏感型(<9.21 ng/mL,n=28).对研究组和对照组儿童禁食12 h后抽取外周静脉血,测定血清血脂水平[总胆固醇(total cholesterol,TC),甘油三酯(triglyceride,TG),高密度脂蛋白(high density lipoprotein,HDL)-C,低密度脂蛋白(low density lipoprotein,LDL)-C)].采用放射性免疫分析法测定血清瘦素水平.采用聚合酶链式反应-限制性片段多态性(polymerase chain reaction restriction fragment length polymorphism,PCR-RFLP)及聚丙烯酰胺凝胶电泳法测定儿童载脂蛋白E基因型.测定两组儿童身高及体重,计算体重指数(body mass index,BMI).结果 与对照组儿童比较,研究组儿童存在血脂代谢紊乱及血清瘦素水平增高,72%肥胖症儿童存在瘦素抵抗.肥胖儿童中瘦素抵抗组血清总胆固醇、甘油三酯和低密度脂蛋白-C显著高于瘦素敏感组,高密度脂蛋白-C显著低于瘦素敏感组.本实验共检出4种载脂蛋白E基因型,分别为E3/3型,E4/3型,E2/3和E4/2型.与健康儿童比较,肥胖儿童ε4等位基因频率增高.携带ε4等位基因个体中,瘦素抵抗型个体与瘦素敏感型个体比较,具有较高的血清总胆固醇、甘油三酯和低密度脂蛋白-C浓度.非携带ε4个体中,瘦素抵抗型个体与瘦素敏感型个体比较,具有较高的血血清总胆固醇、低密度脂蛋白-C浓度,和较低的高密度脂蛋白-C浓度.携带ε4且瘦素抵抗型个体与非携带ε4且瘦素抵抗型个体比较,血清总胆固醇、甘油三酯和低密度脂蛋白-C浓度升高.结论 肥胖症儿童存在瘦素抵抗,其载脂蛋白E基因多态性存在变化,二者可明显影响血脂水平,临床上表现为携带ε4且瘦素抵抗型个体更易发生血脂代谢紊乱.     

北京市城区学龄儿童载脂蛋白E多态性与血脂谱水平的关系

目的 了解载脂蛋白E（ApoE)基因多态性与儿童血脂谱水平的关系。方法 采用聚合酶链反应－限制性内切酶图谱分析法,对307名7－11岁在校儿童进行血脂谱水平测定及ApoE基因多态性检测.结果 307名儿童最常见基因型E3／3检出率为54．7％,其他基因型依次为E4／3（23．8％）、E4／4（9．1％）、E3／2（8．1％）、E4／2（3．9％）、E2／2（0．3％）,不同性别基因型构成的差异无显性;常见等位基因E3频率为70．7％,E4、E2分别为23．0％和6．4％,其中E4频率明显高于国内其他报道,E2频率接近于国内报道;男童E4／3、E3／3基因型总胆固醇（TC,4．19、4．29、3．41mmol/L)、低密度脂蛋白－胆固醇（LDL－C）、载脂蛋白AI（ApoA I)、载脂蛋白B（ApoB)水平高于E3／2基因型,E4／2基因型TC（4．28和3．42mmol/L)、ApoA I水平高于E3／2基因型,女童不同基因型血脂谱水平的差异无显性;高胆固醇组与正常组儿童ApoE-Hha I位点基因型分布比较差异无显性;ApoE2可使TC水平降低0．377mmol/L,LDL-C水平降低0．329mmol/L。结论 ApoE基因多态性与男童血清胆固醇水平相关,E2等位基因携带TC、LDL－C、ApoB水平最低。     

ApoE基因多态性与中国人群MS相关性Meta分析

目的 探讨载脂蛋白E(ApoE)基因多态性与中国人群代谢综合征(MS)各组分的相关性.方法 通过计算机文献检索(中国生物医学文献数据库、中国期刊全文数据库、Medline、Pubmed等数据库)、文献追溯和人工检索的方法,收集1998-2012年公开发表的有关ApoE基因多态性与中国人群MS各组分相关性研究的文献,确定纳入、排除标准,所纳入的文献均为病例-对照研究;运用Meta分析软件Review Manager 4.2对各文献数据进行处理,以病例组及对照组ApoE基因型分布的OR值为效应指标,计算合并OR值及95％ CI,以漏斗图检验人选文献的偏倚,改变统计模型进行敏感性分析.结果 最终共有25篇文献进入系统评价,Meta分析结果表明,高尿酸血症病例与对照组比较,等位基因E2、E3及基因型E2/E2、E3/E3的分布差异均有统计学意义,OR值分别为0.46、1.73、0.05、1.60;其他组分(胰岛素抵抗及糖尿病、肥胖、高血压、血脂异常)病例与对照组的不同等位基因和基因型分布也均有统计学意义.结论 ApoE基因多态性与MS各组分均相关,而不同组分疾病表现在不同的基因多态性上;ApoE基因多态性是患MS疾病的危险因素之一.     

Apolipoprotein E genotypes of fertile and infertile men

Lipid components of spermatozoa have an important role in the functional activity of this cell. The protein, apolipoprotein E (apoE), has a central role in lipid transport. The aim of this study was to investigate the distribution of APOE genotypes, ?3?3, ?3?4, and ?2?3, and the corresponding alleles in fertile and infertile males, and to assess the semen parameters from the patients carrying the different alleles. In addition, the levels of cholesterol, phospholipid, and triacylglycerol in spermatozoa, isolated by PureSperm gradient and from seminal plasma in samples from infertile males was compared with respect to the APOE genotype. APOE genotypes were determined by PCR-RFLP on DNA extracted from peripheral blood leucocytes in 108 fertile and 107 infertile men. There was a significant difference between the distribution of APOE genotypes in fertile as compared to infertile males (χ²?=?9.1, df?=?2, p?=?0.011). The presence of genotype ?3?4 conferred a 3.82 risk factor for male infertility {Odds ratio?=?3.82 (1.46-10, p?=?0.006)}. Our findings showed that the distribution of APOE genotypes and alleles differed between fertile and infertile individuals and may be a risk factor for male infertility. We suggest that the effects of APOE genotypes may be linked to differences in the efficacy of the expressed apoE isoforms in promoting sperm maturation during epididymal transit.     

绝经女性血清瘦素水平与代谢综合征关系

目的 探讨绝经女性血清瘦素与代谢综合征(MS)关系。方法 对沈阳市某社区106名绝经女性进行人体测量、血清瘦素及MS指标测定。结果 因子分析抽出2个公因子,累计方差贡献率为60.7%;核心因子为瘦素-体脂肪,包括瘦素、体脂肪含量、体质指数、腰围;另一因子为MS因子,包括腰围、血压、空腹血糖、甘油三酯、高密度脂蛋白胆固醇。体脂肪含量和收缩压是预测瘦素的独立变量(R²=0.607,P<0.01);瘦素浓度随MS成分数的增多而升高,MS≥3项组瘦素浓度为(11.33±4.51)ng/mL、2项组为(9.21±4.70)ng/mL,均高于≤1项组的(5.54±3.46)ng/mL(均P<0.05);高瘦素水平组(>9.2 ng/mL)MS的危险度是低瘦素水平组(≤9.2 ng/mL)的4倍(OR=4.00,95% CI=1.34~11.97,P<0.05)。结论 绝经女性瘦素与体脂肪含量、收缩压呈正相关,高瘦素水平可增大MS的危险度。     

脂质代谢与妊娠高血压疾病的研究进展

内皮细胞损伤是妊娠期高血压疾病一系列病理生理变化的关键环节,脂代谢与内皮损伤直接相关,因此,推测与脂代谢有关的关键物质脂蛋白脂酶、载脂蛋白E可能在妊娠期高血压疾病中起一定作用.     

长寿与非长寿者子代血脂水平及与apoE基因多态性的关系

目的　探讨遗传基因与血脂谱水平差异的关系。方法　对比分析 7个维吾尔族长寿家系的 86名子孙与 9个维吾尔族非长寿家系的 6 2名子孙的血脂谱水平和apoE等位基因频率分布差异 ,以及apoE基因多态性与血脂谱水平的关系。结果　调整年龄、工作性质后 ,长寿家系子孙不论男女 ,血脂谱中与动脉硬化呈正相关的指标均低于非长寿家系子孙 ,而与动脉硬化呈负相关的指标均高于非长寿家系子孙。其中男性甘油三酯 (1 49± 0 14)mmol/L、apoB(0 83± 0 0 4)g/L均显著低于非长寿家系子孙的 (2 11± 0 17)mmol/L和 (1 0 2± 0 0 5 )g/L ,高密度脂蛋白 胆固醇 /低密度脂蛋白 胆固醇 (0 74± 0 0 4)显著高于非长寿家系子孙 (0 5 9± 0 0 5 ) ,女性apoA/apoB(1 74± 0 0 8)显著高于非长寿家系子孙 (1 42± 0 0 9)。长寿家系子孙中等位基因ε4的相对频率 (5 8)显著低于非长寿家系子孙(16 1)。进一步分析发现 ,ε4等位基因携带者血清中总胆固醇、低密度脂蛋白 胆固醇、apoB水平显著高于其他等位基因携带者。结论　长寿家系子孙血脂谱综合指标优于非长寿家系子孙 ,apoE等位基因多态性可以部分地解释形成这种差异的原因     

Apolipoprotein E polymorphism and plasma lipid, lipoprotein, and apolipoprotein levels in Italian children.

We have investigated the effect of apolipoprotein (apo) E polymorphism on serum lipid, lipoprotein, and apolipoprotein levels in a sample of 195 children, aged 8-11 years, from Sezze, Central Italy. The relative frequencies of e2, e3, and e4 alleles were 0.062, 0.867, and 0.072, respectively. Variation at the apo E gene locus explained 5.1% of the sample variance in serum total cholesterol levels, 7.6% in low-density lipoprotein (LDL) cholesterol levels, 7.3% in apo B levels, and 14.1% in high-density lipoprotein-apo E (HDL-E) levels. The effect of the e2 allele was to lower levels of total cholesterol, LDL-cholesterol, and apo B and to raise levels of HDL-E, while the effect of the e4 allele was the opposite. Variation at the apo E gene locus was not associated with differences in serum triglyceride, HDL-cholesterol, or apo AI levels. The effects of common apo E polymorphisms and genetic variation associated with the PvuII RFLP of the apo B gene on serum apo B levels were additive, explaining 11.3% of the phenotypic variance in this sample. When the effect of apo E polymorphism on serum lipid traits was estimated in boys and girls separately, variation at the apo E gene locus explained 10.4, 13.3, 13.3, and 13.5% of the phenotypic variance in serum total cholesterol, LDL-cholesterol, apo B, and HDL-E levels, respectively, in boys, while in girls only the effect on HDL-E levels (19.3%) reached statistical significance. This study has demonstrated that genetic variations at the apo E locus contribute to the determination of serum lipid, lipoprotein, and apolipoprotein levels in youths and that the effects are gender specific.     

ApoE基因多态性与多因素作用致冠心病研究

目的 探讨冠心病的主要危险因素及载脂蛋白E(ApoE)基因多态性与其它各危险因素之间的交互效应。方法 选择90例冠心病患者(CHD组)及90例非冠心病患者(对照组)，在Logistic回归分析的基础上，分析各因素的比值比以及ApoE基因多态性与其它危险因素之间的交互作用。结果 多因素Logistic回归结果显示，高血压史、高血脂史、肺炎衣原体(Cpn)感染、巨细胞病毒(CMV)感染和ApoE基因多态性为冠心病的危险因素，比值比OR分别为5.04，3.64，2.37，3.51，3.33。ApoE基因多态性与高血压史、高血脂史、Cpn感染、CMV感染间交互作用指数分别为1、73，1.55，1.28，1.20，归因交互百分比分别为40.64％，33.25％，19.01％和15.15％。结论 高血压史、高血脂史、Cpn感染、CMV感染和ApoE基因多态性可能是冠心病的主要危险因素，且ApoE基因多态性与其它危险因素在致冠心病的关系中存在正交互作用。     

Genetic influences on age-related change in total cholesterol,low density lipoprotein-cholesterol and triglyceride levels: Longitudinal apolipoprotein E genotype effects

This study addressed the possible influence of apolipoprotein E (apo E) genotype on age-related changes in total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), and triglyceride (TG) levels in older males. Apo E is a component of LDL, is a ligand for the LDL receptor, and apo E genotype has been consistently associated with variation in mean levels of TC and LDL-C, and also appears to influence TG levels. Using male twins followed longitudinally between mean ages of 48 and 63 years, the change in TC, LDL-C, and TG over time for individuals with the ?3?3 and the ?3?4 genotypes was contrasted. At exam 1 mean TC and LDL-C levels were lower in the ?3?3 group than in the ?3?4 group, but at exam 3 mean TC and LDL-C levels were significantly higher in the ?3?3 group than in the ?3?4 group. The rate of change in TC and LDL-C with age differed significantly between ?3?3 and ?3?4 groups. Results for TG were not statistically significantly. These findings suggest that the apo E genotype effects on risk of coronary artery disease may be age-dependent. This study demonstrates the value of longitudinal studies in refining models for genetic risk factors for disease. © 1994 Wiley-Liss, Inc.     

广西巴马人群血脂与Apo CⅠ基因rs4420638位点多态性关系

目的 探讨载脂蛋白CⅠ(Apo CⅠ)基因的rs4420638位点多态性与广西巴马人群血脂的关系。方法 2008年4-8月从广西巴马县选取健康个体369人作为研究对象,用TaqMan MGB实时荧光PCR技术对研究对象进行基因位点分型,比较巴马人群等位基因频率及血脂水平与对照组的差异。结果 巴马组G等位基因频率为8.3%,低于对照组的13.9%(P=0.002),巴马组高密度脂蛋白胆固醇(HDL-C)水平为1.46 mmol/L,明显高于对照组的1.11 mmol/L(P<0.01);巴马组rs4420638位点G等位基因携带人群血清总胆固醇(TC)水平为4.59 mmol/L,低于对照组的5.23 mmol/L(P<0.05),A等位基因携带人群HDL-C水平为1.46 mmol/L,高于对照组的1.13 mmol/L(P<0.01)。结论 Apo CⅠ基因rs4420638位点多态性可能是影响巴马人群血脂水平的因素。     

吞噬细胞介导的不同脂肪乳剂的脂质过氧化以及维生素E的抗氧化作用

评估维生素E在体外的抗氧化、预防脂质过氧化的作用,比较各种不同类型脂肪乳剂对氧自由基产生及脂质过氧化的影响。取18倒健康志愿静脉血,采用Boyum法提取多核白细胞(PMN)并在体外培养PMN。实验分单纯PMN组,Intralipid组(100％LCT),Vasolipid组(50％LCT,50％MCT)Structolipid组(63％LCT,37％MCT)。各组均分为静止和刺激(用热灭活白色粘株菌刺激PMN)两种状态。采用NBT还原试验测定氧自由基产生量。根据以上分组在体外孵育PMN,并再分为添加Vit E组和无Vit E组。采用比色法-LPO-586测定脂质过氧化的终产物Malonaldehyde(MDA)和4-hydroxyalkenal来计算脂质过氧化程度。刺激组的氧自由基产生量明显高于静止组(0．795±0．180vs.0.405±0．090,P<0．001)。脂肪乳剂可明显降低各组的NBT值,脂肪乳剂浓度与NBT值之间呈负线性相关(r=-0．83,P<0．001)。三组不同脂肪乳剂之间,其NBT值无统计学差异(P>0.05)。刺激组的脂质过氧化产物明显高于静止组(3．15±1.12vs1．34±0．72,P<0.001)。各脂肪乳剂组的脂质过氧化产物明显高于单纯PMN组,而Intratipid组脂质过氧化值高于Vasolipid组和Structolipid组,差异有显性。隧着PMN孵育时间的延长,各组的脂质过氧化值增加,6小时后接近最大值。在体外,维生素E可明显抑制脂质过氧化的产生,8μg／d的维生素E可抑制70-80％PMN所致的脂质过氧化。脂肪乳剂在体外可增加由吞噬细胞介导的脂质过氧化,所增加的脂质过氧化可通过添加维生素E而得到有效的抑制。     

学龄儿童载脂蛋白CⅢ基因变异与血脂谱的关系研究

为了解载脂蛋白CⅢ基因多态性与学龄儿童血脂谱变异的关系 ,对 30 8名在校儿童 (7～ 11岁 )进行血脂水平测定及载脂蛋白CⅢ基因SacⅠ位点多态性检测 (PCR RFLP方法 )。结果表明载脂蛋白CⅢ基因SacⅠ位点杂合突变型 (+ - )检出率为 48 7% ,纯合突变型 (+ +)检出率为 7 5 % ,其等位基因 (+)频率为31 8% ,高于上海 (12 % )及白种人 (6 % ) ,与日本人 (34 % )接近 ,提示遗传变异有人群及种族差异 ;不同基因型儿童血脂水平比较显示 ,纯合突变基因型儿童的TG水平高于野生型 (P <0 0 5 ) ;高甘油三酯组 (+ +)基因型频率为 30 0 % ,高于对照组 6 7% (P <0 0 5 ) ;ApoCⅢ SacⅠ位点 (+)等位基因可使TG水平升高0 0 31mmol L。结果提示 ,载脂蛋白CⅢ基因SacⅠ位点突变与儿童甘油三酯血症水平升高相关     

绝经后妇女胰岛素抵抗与性激素关系研究进展

绝经后妇女性激素的改变与胰岛素抵抗的产生有关。雄激素、性激素结合球蛋白参与了胰岛素抵抗的形成 ,高水平的雌激素可引起胰岛素抵抗 ,生理剂量的雌激素可提高绝经后妇女的胰岛素敏感性 ,降低胰岛素抵抗 ,孕激素与胰岛素抵抗的关系尚需进一步研究 ,该文就近年来绝经后妇女胰岛素抵抗与性激素变化关系的研究进展作以综述。     

新疆维吾尔族载脂蛋白E基因多态性及其与血脂关系的研究

目的：探讨新疆维吾尔族载脂蛋白Ｅ（ａｐｏＥ）基因多态性及其与血脂的关系。方法：采用聚合酶链反应结合限制性片段长度多态性分析（ＰＣＲ－ＲＦＬＰ）检测１６３名维吾尔族和１５０名汉族对照给的ａｐｏＥ基因型,其中１０１名维吾尔族,１４０名汉族测定了血脂和脂蛋白含量。结果：维吾尔族ａｐｏＥ基因型分布以Ｅ３／３为最常见,其次为Ｅ３／４。汉族以Ｅ３／３最常见,其次为Ｅ２／３。ａｐｏＥ三种常见等位基因频率,维吾尔族Ｅ４相对频率显著高于汉族。但维、汉两民族血清中总胆固醇及低密度胆固醇均以Ｅ４基因携带者最高,Ｅ２携带者最低。结论：ａｐｏＥ三种常见等位基因频率分布存在民族差异。但ａｐｏＥ基因多态性与血脂的关系无民族差异。     

Variation at the lipoprotein lipase and apolipoprotein AI-CIII gene loci are associated with fasting lipid and lipoprotein traits in a population sample from Iceland: Interaction between genotype,gender, and smoking status

The effects of polymorphisms in the lipoprotein lipase (LPL) gene (HindIII and S447X) and in the apolipoprotein (apo) AI-CIII gene cluster (G75A and C1100T) on levels of fasting plasma triglycerides, apoCIII, high density lipoprotein cholesterol (HDL-C), and apoAI were examined in 315 healthy men and women from Iceland. Non-smoking and smoking men and women were examined separately because of the strong effects of smoking status and gender on lipoproteins. For the LPL gene, there were no significant associations between plasma traits and genotypes of the S447X polymorphism, but the LPL-HindIII polymorphism was associated with significant effects on levels of all traits, with the effect of genotype on triglycerides and apoAI being modulated by smoking status, (genotype × smoking interaction, P < .02). The H- allele was generally associated with slightly lower levels of apoCIII, with a lowering effect on triglycerides only in smokers and with a raising effect on ApoAI in non-smoking and smoking men and in non-smoking women. For the apoCIII C1100T polymorphism, smoking and non-smoking men with one or more T alleles had levels of triglycerides roughly 10% higher than those with only the C allele (P = .004 overall). In the non-smoking men, nonlinear additive effects were observed with combinations of genotypes at the LPL and apoAI-CIII loci, with the HDL-C and apoAI raising effect associated with the A75 allele and H- allele seen only in those men with both alleles, and the apoCIII raising effect associated with the H+ and T alleles seen only in those with both alleles. Thus, variations at both of the LPL and apoAI-apoCIII loci influence levels of triglycerides, apoCIII, HDL-C, and apoAI, but these effects are strongly modulated by smoking and are different between men and women. The mechanisms for these interactions between smoking or gender and genes are unknown, but future studies should take such interactions into account. Genet. Epidemiol. 14:265–282,1997. © 1997 Wiley-Liss, Inc.     

Association between dietary omega-3 fatty acid intake and depression in postmenopausal women

BACKGROUND/OBJECTIVESThis study aimed to analyze the association between dietary omega-3 fatty acid intake and depression in postmenopausal women using data from the Korea National Health and Nutrition Examination Survey (KNHANES) VI.SUBJECTS/METHODSThe KNHANES is a cross-sectional nationwide health and nutrition survey. Dietary data, including omega-3 fatty acids, were assessed using the 24-h recall method. Depression was evaluated using a survey questionnaire. The association between dietary omega-3 fatty acids and depression was evaluated using multivariate logistic regression analysis. Depression, according to the dietary omega-3 fatty acid intake, was expressed as the odds ratio (OR) with a 95% confidence interval (CI). A total of 4,150 postmenopausal women were included in the analysis.RESULTSIn the fully-adjusted model, the group with the highest dietary omega-3 fatty acid intake significantly showed lower prevalence of depression than the group with the lowest intake (OR, 0.52; 95% CI, 0.33–0.83); a significant linear trend was detected (P for trend = 0.04). According to the dose-response analysis using cubic restricted spline regression, this association was linear and monotonic (P for non-linearity = 0.32).CONCLUSIONSIn this study, the dietary omega-3 fatty acid intake in postmenopausal women was inversely proportional to depression in a dose-response manner. Large cohort studies are needed to verify the causality between omega-3 fatty acids and depression in Korean postmenopausal women.     

单纯性肥胖儿童脂代谢与LEPR及ApoE基因多态性的相关性研究

目的探讨单纯性肥胖儿童脂代谢与LEPR及ApoE基因多态性的相关性。方法常规苯酚-氯仿法抽提基因组DNA,用聚合酶链式反应-限制性片断长度多态性（PCR-RFLP）及聚丙烯酰胺凝胶电泳法分析135例单纯性肥胖儿童及84例健康儿童LEPR基因的第20外显子和ApoE基因的第4外显子基因型及等位基因变异频率,并测定两组儿童血清甘油三酯（triglyceride,TG）、总胆固醇（total choles-terol,TC）、高密度脂蛋白胆固醇（high density lipoprotein cholesterol,HDLC）、低密度脂蛋白胆固醇（lowdensity lipoprotein cholesterol,LDLC）浓度,并测定两组儿童身高和体重,按公式计算其体重指数（bodymass index,BMI）。结果本实验检出3种LEPR基因第20外显子的基因型,分别为：A/A型、A/G型、G/G型;4种ApoE基因第4外显子的基因型,分别为：E3/3型、E4/3型、E2/3型、E4/2型,未检出E2/2、E4/4纯合型。与健康儿童相比,单纯性肥胖儿童LEPR基因第3057位核苷酸G→A突变频率及ApoE基因第3934位核苷酸T→C（ε4）突变频率增高（P〈0.05）。与非ε4携带ApoE基因型个体比较,携带ε4基因的G/A型肥胖儿童的血TG,LDLC浓度升高,BMI增高;携带ε4基因的A/A型单纯性肥胖儿童血TC,TG,LDLC浓度升高,BMI亦增高,血HDLC下降,差异有显著意义（P〈0.05）。结论本地区健康及单纯性肥胖儿童存在LEPR基因第20外显子及ApoE基因第4外显子,存在基因多态性变化。LEPR基因与ApoE基因变异,可以明显影响儿童血脂及BMI,且有一定的协同作用。     

A 12-week worksite health promotion program reduces cardiovascular risk factors in male workers with the apolipoprotein E2 and apolipoprotein E3 genotypes,but not in apolipoprotein E4 genotype

Worksite health promotion programs focusing on diet and lifestyle modification have been shown to improve health outcomes in workers. The purpose of this study was to investigate whether a 12-week worksite health promotion program shows different response of cardiovascular risk factors in subjects according to apolipoprotein E (Apo E) genotype and obesity level in 141 male Korean industrial workers. We hypothesized that the health changes of a 12-week intervention may not be the same within Apo E genotypes in nonobese and obese subjects. They received 5 face-to-face meetings based on their health profiles. In obese group carrying Apo E3 genotype, body mass index, body fat (%), waist circumference, waist-hip ratio, and systolic blood pressure were decreased, as well as intakes of energy (P = .000) and carbohydrate (P = .005). High-density lipoprotein cholesterol (P = .004) level was improved in individuals with the Apo E2 genotype. These beneficial effects were only observed in individuals with the Apo E2 or Apo E3 genotype. Multiple linear regression revealed that obesity was strongly correlated with waist circumference (P = .002), plasma total cholesterol (P = .037), and changes in dietary cholesterol intake (P = .011) in individuals with the Apo E3 genotype, whereas only changes in dietary fat intake (P = .044) was correlated in those with the Apo E4 genotype. Overall, the results of this study suggest that a health promotion program can be a useful method of improving cardiovascular risk factors and dietary intake in industrial workers with certain genotypes only. Therefore, further research is needed to develop a tailored, long-term worksite health promotion program based on genetic background.     

内蒙古包头市区肥胖儿童ApoE基因多态性及血脂水平

目的研究单纯性肥胖儿童ApoE基因多态性及血脂水平。方法检测来自本地区两所中学12～14岁60例肥胖儿童(60例对照)血脂,采用PCR限制性内切酶片段长度多态性(PCR-RFLP)技术分析ApoE基因多态性。结果本实验共检出4种ApoE基因型,分别是E3/3、E3/2、E3/4、E2/4,未检出E2/2、E4/4型。肥胖组与对照组等位基因ε2、ε3、ε4频率分布不均,ε3发生频率最高。与健康组儿童比较,肥胖组儿童ε4等位基因发生频率增高,差异有统计学意义(P﹤0.05)。肥胖组TC、TG、LDL-C水平由低到高依次为E2/X﹤E3/3﹤E4/X,HDL-C水平E2/X﹥E3/3,差异均有统计学意义(P﹤0.05)。对照组TC水平差异同肥胖组,LDL-C水平E2/X﹤E3/3,TG、HDL-C水平差异无统计学意义。结论 ApoE基因多态性影响肥胖儿童血脂水平。