Fractionation techniques such as temperature rising elution fractionation or crystallization analysis fractionation fail to fractionate the ethylene–propylene (EP) copolymers, which are a component of high‐impact polypropylene (hiPP), according to their chemical composition. High‐temperature high‐performance liquid chromatography separates blends of EP‐copolymers and polypropylene. The elution volume of the EP‐copolymers depends linearly on the average content of ethylene. The separation according to the chemical composition was hyphenated with size exclusion chromatography. In this way, the relationship between the distribution with regard to chemical composition and molar mass, that is, the full chemical heterogeneity of hiPP, was revealed for the first time. 相似文献
ABSTRACT: In the present work, 500 and 50,000 porcine zonae pellucidae were solubilized using Lithium-3,5-diiodosalicylate. The zona antigens were purified by immunoaffinity chromatography (IAC) on immobilized antizona immunoglobulin G (IgG). The antizona-IgG was raised by immunization of female rabbits with 500 heat-solubilized porcine zonae. Four antigens could be detected following IAC: ZP I/1 (Mr = 42,000), ZP II/1 (Mr = 67,000), ZP II/2 (Mr = 32,000), ZP III/1 (Mr = 17,000). In a parallel experiment, 50,000 zonae were solubilized in a similar manner and the mixture was analyzed by high-pressure liquid chromatography (HPLC) using a protein column. Altogether, 9 protein peaks that contained the antigens ZP I/1, ZP II/1, ZP II/2, and ZP III/1 could be detected following HPLC. The carbohydrate composition is characteristic for O-glycosidic-glycoproteins. ZP II/1 and ZP II/2 are probably in close association within the zona. Based on the reaction of the antigens with antibodies induced by intact and heat-solubilized zonae, it is postulated that only ZP I/1 and ZP II/l are expressed on the surface in intact zonae. 相似文献
A method of microgel chromatography of proteins on a Sephadex G-75 (superfine) column with a working volume of 110–150 l is described. By means of the method molecular weight fractionation of proteins can be carried out within the range 10–7–10–8 g in the course of 45–60 min.Microchemistry Room, Laboratory of Physiological Mechanisms of Memory Control, Institute of Experimental Medicine, Academy of Medical Sciences of the USSR, Leningrad. (Presented by Academician of the Academy of Medical Sciences of the USSR A. N. Klimov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 83, No. 5, pp. 635–636, May, 1977. 相似文献
A size exclusion chromatography (SEC)‐gradient method is developed allowing poly(n‐butyl acrylate‐stat‐acrylic acid)s to be separated with respect to content of acrylic acid over the complete composition range. After setting up the chromatographic method, samples that, according to the amounts of charged monomers, are expected to have identical chemical composition are compared. The chromatograms reveal differences in elution volume, which, by 1H NMR spectroscopy, can be partially traced back to differences in polymer composition. In addition, samples of similar compositions prepared in different solvents exhibit differences in chemical composition distribution.
Two‐dimensional chromatographic methods were developed using LC‐CC in the first and SEC in the second dimension. These methods were applied for the investigation of PS‐b‐PI diblock copolymers synthesized by different approaches: sequential living anionic polymerization and coupling of living precursor blocks. The first dimension separates according to the individual block length of PS or PI blocks, whereas the second dimension separates with respect to the total molar masses of components. 2D‐LC analysis provides information on the purity of the reaction products, the presence of by‐products, the chemical compositions and the molar masses of all product components. The accuracy and selectivity of 2D‐LC is discussed.
Chitosan is obtained by N-deacetylation of chitin, which is the second most abundant natural biopolymer. In this study, an improved preparation and characterisation of a chitosan-based immunoaffinity chromatography (IAC) column were performed. The immunoaffinity adsorbent was prepared by covalently coupling monoclonal antibody (mAb) against methandrostenolone (MA) to glutaraldehyde cross-linked chitosan (chitosan CL). Scanning electron micrograph of chitosan CL indicated that the shape of the particles was spherical with a diameter range from about 300 to 500 µm. Infrared spectral analysis suggested that the immunoaffinity adsorbent was successfully prepared by coupling antibody with chitosan CL. For chromatographic extraction, 90% methanol was selected as eluant. Under optimum conditions, the maximum binding capacity of the IAC column was 3900 ng MA/mL adsorbent. The average recovery of 50, 250 and 500 ng of MA standards from IAC columns was 96.9% with a relative standard deviation among columns of 1.48%. After eight uses, the extraction recovery of the IAC column remained 82.1%. To further verify the effect of matrices on the extraction efficiency, the IAC columns were challenged with MA-fortified animal tissue and feed samples. Recoveries were 84.9–87.1%, demonstrating the effectiveness of these IAC columns for sample clean-up in MA residue determination. 相似文献