共查询到20条相似文献,搜索用时 62 毫秒
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目的:完善灯盏生脉胶囊的薄层鉴别.方法:采用薄层鉴别以野黄芩苷、五味子乙素为对照品。结果:供试品色谱中,在与对照品色谱相应的位置上,显相同颜色的荧光斑点。结论:方法专属、斑点清晰、分离度好,可用于灯盏生脉胶囊的薄层鉴别。 相似文献
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Methadyl acetate was metabolized by microsomal preparations of rat liver to yield nor-methadyl acetate and 6-(dimethylamino)-4,4-diphenyl-3-heptanol. The identification and separation of these three compounds was established by TLC, using iodoplatinate spray as a visualizing agent. 相似文献
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Yamakawa K Nishitani K 《Yakushigaku zasshi. The Journal of Japanese history of pharmacy》2003,38(1):28-41
The separation of organic compounds has been carried out using the method of distillation and recrystallization since the 17th century.1st separation technology innovation (1950s to 1960s). After World War II, the science of separating organic compounds evolved to the use of paper or column chromatography. 2nd separation technology innovation (1960s to 1980s). In the 1960s, thin-layer chromatograpy (TLC) was developed. TLC is a convenient analytical technique for organic compounds. Separation methods for organic compounds using column chromatography and preparative TLC were carried out as practical applications. Gas chromatography (GC) was also introduced in the 1960s. GC is a useful analytical method for a mixture of various volatile organic compounds. Many GC instruments with new kinds of sensors were developed. Gas chromatography of nonbenzenoid organometallic compounds and metal complexes was successfully conducted by the author. 3rd separation technology innovation (1980s to 1990s). In the 1980s, high-performance liquid-chromatography (HPLC) was developed. HPLC analytical methods were useful for various kinds of organic compounds. In the 1990s, LC-MS and LC-NMR instruments were introduced for biomedical products. 4th separation technology innovation (1990s to present). Several new ionization methods for mass-spectrometry (electrospray, matrix-aided lazer desorption ionization, etc) are applied for proteins and biomedical products. Several new separation technologies (supercritical field chromatography, capillary electrophoresis, field-flow fractionization, etc.) are now being developed. 相似文献
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Y Okumura T Takei M Sakamoto T Ishikura Y Fukagawa 《The Journal of antibiotics》1979,32(10):1002-1010
The high response of our isolate of Streptomyces griseoviridus was exploited to provoke the synthesis of new viridogrisein homologues by adding various amino acids to the culture medium in an attempt to replace the alanine, sarcosine, leucine and phenylsarcosine moieties of virdogrisein. Among the amino acids added, L- and DL-alpha-amino-n-butyric acid and L-methionine gave new TLC spots which we named neoviridogriseins I and III and neoviridogrisein VII, respectively. The structures of neoviridogriseins I and III were elucidated: In both compounds, the alanine moiety of viridogrisein is replaced by a L-alpha-amino-n-butyric acid residue; furthermore, in neoviridogrisein I, the allo-hydroxy-D-proline is replaced by D-proline. 相似文献
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S R Sun 《Journal of pharmaceutical sciences》1978,67(10):1413-1415
A sensitive fluorescence--TLC densitometric procedure was developed for the specific determination of diazepam (I) and its two metabolites, desmethyldiazepam (II) and oxazepam (III), in serum. After extraction from serum with benzene, the compounds were separated by TLC and converted with a sulfuric acid spray to greenish-yellow fluorescence spots with Rf values of 0.72, 0.43, and 0.17, respectively. Quantitation of the TLC plate was accomplished by scanning with a densitometer at 380 nm. The sensitivities of the assay were 18 (I), 6 (II), and 5 (III) ng/ml of serum. This procedure was also was applicable to other 1,4-benzodiazepines in biological fluids. 相似文献
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目的建立定性鉴别紫草总色素β-环糊精包合物有效成分的薄层色谱(TLC)法和测定左旋紫草素含量的高效液相色谱(HPLC)法。方法采用TLC法进行定性鉴别,展开系统为环己烷-甲苯-乙酸乙酯-甲酸(5∶5∶0. 5∶0. 1,V/V/V/V);采用HPLC法测定包合物中有效成分左旋紫草素的含量,流动相为甲醇-0. 025 mol/L磷酸(82∶18,V/V)。结果 TLC鉴别中,供试品溶液与对照品溶液在相应位置上显相同颜色斑点,且斑点清晰,阴性对照无干扰;HPLC图中,供试品溶液与对照品溶液色谱出峰时间一致,分离度好,阴性对照无干扰,含量测定中有效成分的线性关系良好,平均加样回收率为102. 09%,RSD为1. 87%(n=6)。结论该方法操作简便、结果准确、专属性强、重复性好,可用于该产品的质量控制。 相似文献
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Solid-supported reagents for multi-step organic synthesis: preparation and application 总被引:1,自引:0,他引:1
Ley SV Baxendale IR Brusotti G Caldarelli M Massi A Nesi M 《Il Farmaco; edizione pratica》2002,57(4):321-330
Since the early days of combinatorial chemistry solid-phase organic synthesis has been the method of choice for the production of large libraries. Solution-phase synthesis is again gaining importance especially for the synthesis of parallel arrays of smaller, focussed libraries containing single compounds with high degrees of purity. In the field of solution-phase library generation, the use of solid-supported reagents, catalysts and scavengers is emerging as a leading strategy, combining the advantages of both solid-phase organic synthesis (e.g. allowing the employment of an excess of reagent without the need for additional purification steps) and solution-phase chemistry (e.g. the ease of monitoring the progress of the reactions by applying LC-MS, TLC or standard NMR techniques). An account of some of the most recent advances in this area of research will be presented. 相似文献
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目的:考查玉叶清火胶囊中玉叶金花的鉴别方法。方法:通过改变提取方法、洗脱剂、展开剂选择薄层色谱法(TLC)中的最佳实验条件。结果:经大孔树脂处理,20%~30%之间的乙醇洗脱,以三氯甲烷-甲醇-水(13:6:2)10℃以下放置分层的下层溶液为展开剂得到的薄层色谱图中,玉叶金花与对照药材色谱在相应的位置上,显相同颜色的斑点,且斑点清晰,分离效果好,阴性对照无干扰。结论:实验证明此方法简便、快捷,能准确鉴别出玉叶清火胶囊中的玉叶金花。 相似文献
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目的:建立胃乐舒颗粒的质量控制方法。方法:采用薄层鉴别法(TLC)对制剂中主要药味延胡索、旋覆花、山楂、厚朴进行定性鉴别;以HPLC法测定延胡索中延胡索乙素的含量:色谱柱为Agilent SB-C18(4.6 mm×250 mm,5 μm),流动相为乙腈-0.1%磷酸溶液(45:55,磷酸溶液用三乙胺调pH到6),检测波长为226 nm。结果:在薄层色谱中,各供试品色谱,在与对照品或对照药材相应的位置上显相同颜色的斑点,阴性对照无干扰;延胡索乙素在0.050~0.657 μg范围内线性关系良好,r=1.000 0。结论:该方法操作简单,重复性好,能够有效地控制胃乐舒颗粒的质量。 相似文献
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Hosomi K Okuno A Umetani Y Araya T Matsuyama K Haginaka J Mifune M Saito Y 《Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan》2004,124(9):587-598
We studied the color change of phenothiazines and metal-containing drugs after compound formation, followed by use of FT-Raman spectrocopy to observe any structural changes in them. When 6 phenothiazines (thioridazine hydrochloride, prochlorperazine maleate, levomepromazine maleate, chlorpromazine phenolphthalinate, fluphenazine maleate and perphenazine fendiate) formed compounds with natural aluminum silicate, the color change was accompanied by a shift of FT-Raman signals. These changes could be attributed to the structural changes of phenothiazines. This present observation can be then used in advance to avoid coloration of phenothiazines during preparative procedures with metal-containing drugs such as antacids. 相似文献
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Sixteen visualizing agents, thirteen of which were a group of alkacemetric indicators were used for the detection of selected drugs (being phenolic compounds): adrenaline, dopamine, phenylephrine, metaraminol, fenoterol, and bithionol. Visualizing effects for the drugs investigated after their TLC separation were estimated on silicagel (A), mixture silica gel/kieselguhr (B), and on polyamide (C). The best separations and the most positive visualising effects were obtained on adsorbent A, and the least on absorbent C. On adsorbent A, the most profitable detectability equal to 100 ng, was obtained also for adrenaline, dopamine and fenoterol with the application of basic solution of bromocresol green and brilliant cresyl blue as visualizing agents. 相似文献
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目的:对金果饮的质量标准进行制定。方法:对玄参、西青果、陈皮建立薄层色谱的鉴别方法;采用高效液相色谱法测定橙皮苷的含量。用Agilent SB C18柱(4.6mm×250mm,5μm),以甲醇-6%乙酸溶液(35∶65)为流动相;检测波长为283nm,流速1mL·min-1。结果:薄层色谱鉴别中,供试品在与对照药材色谱相应的位置上,显示相同颜色的斑点,斑点清晰,分离效果好,阴性无干扰;含量测定法中,橙皮苷在36.35~726.95ng(r=0.9999,n=5)范围内呈良好的线性关系,平均加样回收率为98.58%(RSD为1.85%)。结论:建立的方法简便、准确、专属性强、重复性好,能有效用于金果饮的质量控制。 相似文献
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Misiuk W Kuźmicka L Mielech K Puzanowska-Tarasiewicz H 《Acta poloniae pharmaceutica》2001,58(6):421-425
Iron (III) chloride and potassium hexacyanoferrate (III) have been tested as reagents for the determination of promazine hydrochloride and perazine. The methods are based on the oxidation of phenothiazines by FeCl3 and K3[Fe(CN)6] in perchloric acid medium. The optimal conditions for the formation of oxidation products of promazine and perazine were examined. The absorption spectra in the UV-VIS region were recorded. 相似文献
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目的拓宽薄层鉴别检测途径,提高中药成方制剂中的薄层鉴别增订率。方法将各药材的样品溶液点于薄层板上,展开后,进行不同方式的增荧光反应研究。结果药材中多种无检测信息的成分呈现出不同颜色的荧光,斑点清晰,检测灵敏度提高,信息量增大。结论本研究有效拓宽了薄层鉴别检测途径,使多种无检测信息的中药材,能够被高灵敏度、准确地进行定性和定量控制。 相似文献
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Structure of the Products of Reactions of Cotarnine and Hydrastinine with CH Acidic Compounds and Acetic Anhydride The products resulting from reactions of cotarnine and hydrastinine with CH acidic compounds are cyclic derivatives with substituents at position 5. The reaction of carbinolamines with acetic anhydride causes ring opening. Cyclic ?O-acetylcotarnine”? was identified as the opened-chain acetylcotarninacetic acid. 相似文献