A Study on Protective Action of Astragalus Injection(黄芪注射液)in Ouabain-Induced Cardiac Toxicity of Guinea Pigs

ＡｓｔｒａｇａｌｕｓＩｎｊｅｃｔｉｏｎ（ＡＩ）ｈａｓｂｅｅｎｗｉｄｅｌｙｕｓｅｄｔｏｔｒｅａｔｃｈｒｏｎｉｃｃｏｎｇｅｓｔｉｖｅｈｅａｒｔｆａｉｌｕｒｅ（ＣＨＦ）．Ｒｅｃｅｎｔｌｙ,ＹＡＮＧｅｔａｌ．ｈａｖｅｒｅｐｏｒｔ ｅｄｔｈａｔＡＩｗａｓｃｌｉｎｉｃａｌｌｙｅｆｆｅｃｔｉｖｅｉｎｄｅｃｒｅａｓｉｎｇｔｈｅｉｎｃｉｄｅｎｃｅｏｆｄｉｇｉｔａｌｉｓｍ（1）．ＡｌｔｈｏｕｇｈＡＩｈａｓｂｅｅｎｃｌａｉｍｅｄｔｏｉｎｈｉｂｉｔｄｉｇｉｔａｌｉｓｍ,ｉｔｓｍｅｃｈａ ｎｉｓｍｏｆａｎｔｉ ｄｉｇｉｔａｌ…     

Clinical Study on Protective Effect of Astragalus Injection Plus Ligustrazin in Patients with Myocardial Ischemia Reperfussion Injury

Objective: To investigate the mechanism of protective effect of Astragalus (As) plus ligustrazine (Lt) against myocardial ischemia reperfusion injury (MIRI) during open heart surgery of cardiopulmonary bypass, and the treating principle of MIRI in TCM.Methods: Twenty-four patients were randomly divided into three treated groups and treated with As injection, Lt injection and both (As + Lt) respectively, 6 in each group, and the remainder 6 were taken as control group. Blood samples of them were collected before anesthesia (T1), 10 minutes after occlusion of aorta (T2), 10 minutes(T3) and 30 minutes(T4) after the release, and end of operation (about 180 minutes after release, T5) respectively to test the levels of aspartate aminotransferase ( AST ), lactate dehydrogenase (LDH), creatine kinase (CK), MB isoenzyme of CK (CK-MB), malondialdehyde (MDA) and the activity of Superoxide dismutase ( SOD ), nitric oxide ( NO ), nitric oxide synthetase (NOS).Results: The levels of AST, LDH, CK were reduced in the treated groups, showing significant difference (P< 0.05 or P < 0. 01 ) as compared with those in the control groups. Among them the effect in the AS + Lt group was the best. And NO activity in As group at T5 and As + Lt group at T4, T5 was higher than that in the control group.Conclusion : The mechanism of MIRI is Qi deficiency and blood stasis in TCM, its treating principles should be promoting Qi and removing blood stasis. According to the authors’ study, combined As + Lt therapy is more effective in protecting against MIRI than that using the 2 medicines separately.     

黄芪（Astragalus）

又名 Beg kei, Bei Qi, Buck Qi, Huang Qi, Huang qi, Hwanggi, Membranous Milk Vetch, Milk Vetch, Mongolian Milk, Ogio 植物名Astragalus membranaceus ; A. mongholicus.活性成分与药理作用黄芪的药用部位是根，含有40种以上皂苷(如黄芪苷)，此外还有黄酮类、多糖类、微量矿物质、氨基酸和香豆素类。黄芪是一种抗氧剂，抑制自由基的产生，增加超氧化物歧化酶，减少脂质过氧化。黄芪具增强免疫系统、肝和心血管系统的作用，通过干扰素的增效作用改善免疫应答。     

Observation on Efficacy of Astragalus Injection in Treating Diabetic Nephrotic Edema

Ａｔｔｈｅｍｉｄ ｌａｔｅｓｔａｇｅｏｆｄｉａｂｅｔｉｃｎｅｐｈｒｏｐａ ｔｈｙｍａｓｓｉｖｅｐｒｏｔｅｉｎｕｒｉａ，ｈｙｐｏｐｒｏｔｅｉｎｅｍｉａａｎｄａｎａｓａｒｃａｗｏｕｌｄｄｅｖｅｌｏｐｗｈｉｃｈｉｓｎｅｐｈｒｏｔｉｃｓｙｎ ｄｒｏｍｅ．Ｄｉｕｒｅｔ     

Clinical study on effect of Astragalus Injection (黄芪注射液) and its immuno-regulation action in treating chronic aplastic anemia

Objective:To observe the clinical effect of Astragalus Injection(黄芪注射液,AI) and its immuno-regulatory action in treating chronic aplastic anemia(CAA).Methods:Sixty patients with CAA were randomly assigned to two groups equally,both were treated with Stanozolol three times a day,2 mg each time through oral intake,but AI was given additionally to the patients in the treated group once a day via intravenous dripping.All were treated for 15 days as one therapeutic course and the whole medication lasted for more than 4 months totally,with follow-up adopted.The clinical effi cacy was estimated and the changes of T-lymphocyte subsets in peripheral blood as well as the serum levels of tumor necrosis factor-α(TNF-α) and interleukin-2(IL-2) were observed.Results:The total effective rate in the treated group was 83.3%(25/30),which was higher than that in the control group 66.7%(20/30),showing significant difference between them(P<0.05).Levels of hemoglobin,WBC,reticular cell and platelet were elevated in both groups after treatment,but the improvement was signif icantly better in the treated group than that in the control group with respect to the former three indexes(P<0.05).The level of CD4 increased and that of CD8 decreased signifi cantly after treatment in the treated group(P<0.05),which showed significant difference as compared with those in the control group(P<0.05).Levels of serum TNF-α and IL-2 lowered after treatment in both groups,but signifi cance only showed in the treated group(P<0.05).The degree of proliferation in bone marrow got raised signifi cantly and the percentage of non-hemopoietic cells reduced signifi cantly in the treated group after treatment,also showing significant difference to those in the control group(P<0.05).Conclusion:AI could promote the recovery of hemopoietic function,which might be through improving T-lymphocyte subsets and reducing the release of negative regulatory factors such as TNF-α and IL-2 to alleviate the inhibition on hemopoietic function.     

Study on the Toxicity-reducing andEfficacy-enhancing Action of ChineseMedicine in Radio-chemotherapy

Malignant tumor seriously jeopardizes the health of mankind, and its incidence tends to increase gradually. According to the latest statistics, there are about 1. 8 to 2 million new malignant tumor patients per year in China. Since most patients     

A Study of the Mechanism of Yinzhihuang（菌栀黄）Injection in the Treatment of Infantile Hepatitis Syndrome

ＡＳｔｕｄｙｏｆｔｈｅＭｅｃｈａｎｉｓｍｏｆＹｉｎｚｈｉｈｕａｎｇ（菌栀黄）ＩｎｊｅｃｔｉｏｎｉｎｔｈｅＴｒｅａｔｍｅｎｔｏｆＩｎｆａｎｔｉｌｅＨｅｐａｔｉｔｉｓＳｙｎｄｒｏｍｅＤｏｎｇＹｏｎｇ－ｓｕｉ（董永绥）；ＨｕａｎｇＺｈｉ－ｈｕａ（黄志华）；...     

Study on Antiplatelet Mechanism of Ligusticum Wallichii Injection

ＳｔｕｄｙｏｎＡｎｔｉｐｌａｔｅｌｅｔＭｅｃｈａｎｉｓｍｏｆＬｉｇｕｓｔｉｃｕｍＷａｌｌｉｃｈｉｉＩｎｊｅｃｔｉｏｎＬａｉＦｕ－ｓｈｅｎ（赖福生），ＳｈｉＹｉｎ－ｍｉａｎ（史荫绵），ＣｈｕＺｈｏｎｇ－ｌｕ（储钟禄），ｅｔａｌ（ＮａｎｊｉｎｇＨｏｓｐｉ...     

Effect of Astragalus Injection on Levels of Blood Selenium and Immunity Function in Children with Viral Myocarditis

Objective: To observe the effect of Astragalus Injection (AI) on levels of blood selenium (Se)and cytokines, and T cellular immune function with viral myocarditis (VM) in children. Methods: Eighty children with VM were randomly divided into 2 groups. The control group consisted of 38 patients, to whom conventional therapy, including energy mixture, vitamin C and coenzyme Q10, etc. were given. The treated group (n=42), to whom combination therapy of conventional therapy and AI were given. The levels of blood Se and cytokine, including interleukin-1 (IL-1), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and also evaluation of T lymphocyte subsets and cardiac function were observed. Results: The results showed that after treatment, the levels of blood Se were significantly higher (P＜0.01), while IL-1, IL-6 and TNF-αwere significantly lower (P＜0.01) than those before treatment in the control group. The left ventricular end diameter (LVED) were significantly decreased (P＜0.01), left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) were significantly increased than those before treatment in the treated group(P＜0.01, P＜0.05). T lymphocyte subsets got normalized (P＜0.01), and compared with the control group, the difference was significant (P＜0.01). Conclusion: Astragalus membranaceus possesses anti-viral effect, adjusts the balance of cytokine and T cellular immunity, and improves the clinical manifestation and cardiac function. It is an effective approach in treating viral myocarditis.     

Effect of Astragalus Injection on serious abdominal traumatic patients’ cellular immunity

Objective: To explore the change of serious abdominal traumatic patients’ cellular immunity and the effect of Astragalus Injection (AI) on it.Methods: Sixty-three serious abdominal traumatic patients were randomly assigned into two groups, the conventional group and the treated group, patients in the conventional group were given conventional treatment, while others in the treated group were given conventional treatment as the basis, with AI 20 ml was added into 250 ml of 5% glucose solution given through intravenous dripping, and then on the first day and 14th day, their T cell activated antigens as well as that of 10 healthy subjects were monitored.Results: On the first day, in the conventional group and treated group, the levels of CD ₃ ⁺ , CD ₄ ⁺ , CD ₄ ⁺ /CD ₈ ⁺ , CD ₁₆ ⁺ , CD ₆₉ ⁺ and CD ₃ ⁺ /homologous leucocytic antigen-DR (HLA-DR⁺) were apparently lower than those in the healthy group (P<0.05), while the CD ₈ ⁺ was significantly higher than that in the healthy group (P<0.05), and there was no significant difference between the conventional group and the treated group (P>0. 05); on the 14th days, the levels of CD ₃ ⁺ , CD ₄ ⁺ , CD ₄ ⁺ /CD ₈ ⁺ , CD ₁₆ ⁺ , CD ₆₉ ⁺ and CD ₃ ⁺ /HLA-DR⁺ of the treated group got closed to healthy subject value, and got even higher than those of conventional group (P<0.05); CD ₈ ⁺ got close to that of healthy subjects, while obviously lower than that of conventional group (P<0.05).Conclusion: After serious abdominal trauma, cellular immunity lowered, auxiliary use of AI was beneficial to the restoration of cellular immunity.     

Effects of Intraventricular Injection of Calmodulin Antagonist （Trifluoperazine） on Pregnancy in Rats

Injection of TFP (a specific antagonist of CaM) into the lateral ventricles of the rat brain on the fourth day of pregnancy causes marked antifertility effect in 83% of the rats (0% in the control group). Examination with the pontamone blue reaction for the implantation elucidated that the antifertitity effect was due to the blockage of implantation of the follicle. Injection of TFP into the lateral ventricles of the brain markedly reduced the concentration of CaM in hypothalamus, ovary and uterus. Serum progesterone was also reduced. However, injection glven on day 7 was ineffective to terminate the pregnancy. Injection given on certain time of pregnancy was able to reduce the cellular CaM content and to cause antifertitity. This finding demonstrated that CaM played an important rote during the course of pregnancy.     

Effect of Astragalus Injection on Serious Abdominal Traumatic Patients’ Cellular Immunity

After serioustrauma,thei mmunefunction ofpatients was inhibited, particularly the cellulari mmunity function,not only increased the infec-tionincidence rate,but also,after the infectionoccurrence,was apt toleadto sepsis and multi-ple organ dysfunction syndromes ( MODS) ,which was the main cause of deathfor traumaticpatientsin thelate stage(1).The present study,onthe basis of superiority of Astragalus injection(AI) in actingto strengthen and regulatethei m-munityfunction,triesto explorethe ef…     

ClinicalControlled Study of Shenmai Injection(参麦注射液)and Aminophylline on DiaphragmFatigue

Objective: To compare the therapeutic effect of Shenmai injection (SM) and aminophylline (AP) on diaphragm fatigue in chronic obstructive pulmonary disease ( COPD ).Methods: Sixty-four COPD patients with respiratory failure and diaphragm fatigue were randomly divided into 2 groups. The SM group (n=33) was treated with oxygen inhalation, anti-infection agents, and SM injection (SM 40ml + 10% glucose 100ml). The AP group (n=31) was treated by the same comprehensive treatment but with AP 0. 25g instead of SM. The initial time of diaphragm fatigue disappearance, 24 hrs maintaining effect and the arterial blood gas analysis were observed.Results: The effect in 30 min and maintaining effect in 24 hrs in SM group were better than those in AP group. In both groups, PaO₂ increased and PaCO₂ decreased.Conclusion: The therapeutic effect of SM on COPD patients with respiratory failure and diaphragm fatigue was better than those of AP.     

Clinical Study on Effect of Shenmai Injection in Treating Congestive Heart Failure

Objective: To observe the therapeutic effect of Shenmai Injection (SI) in treating congestive heart failure (CHF). Methods: The changes in cAMP, cGMP, serum cardiac troponin T (cTnT, a specific marker reflecting myocardial injury), creatine kinase (CK) and creatine kinase isoenzyme (CK-     

Clinical Study on Effect of Shenfu Injection(参附注射液)in Treating Senile Cerebral Infarction

Objective:To explore the clinical effectand mechanism of Shenfu Injection(SFI)intreating senile cerebral infarction.Methods:Eighty-two aged patients with cerebral in-farction were randomly divided into the trea-ted group treated with SFI and the controlgroup treated with Venoruton.The clinicaleffect was observed,the hemorrheologic and     

Clinical Study on Shenmai Injection in Promoting Postoperative Recovery in Patients of Breast Cancer

Clinical Study on Shenmai Injection in Promoting Postoperative Recovery in Patients of Breast Cancer@刘 鹏 @曹迎明 @乔新民 @杨德启 @周 波 @邵 晖     

A Clinical Study on the Effects and Mechanism of Xuebijing Injection(血必净注射液)in Severe Pneumonia Patients

Objective: To observe the effects of Xuebijing Injection (血必净注射液) in patients with severe pneumonia, and to explore the mechanism. Methods: Eighty cases of severe pneumonia are randomly assigned to the Xuebijing treatment (forty cases) and the control group (forty cases), with the same routine therapy provided in both groups. Clinical effective rates, inflammatory factors and organ function were observed in both groups. Results: The effective rate was higher in Xuebijing group than that of the control group (80.0% vs. 67.5%, P<0.05). As compared with the control group, the LDH, α1-AG, α1-AT levels and the peak body temperature decreased markedly with the Xuebijing treatment going, and the secretion of TNF-α, IL-6, IL-8 was suppressed in Xuebijing group; but no significant difference was found in leptin level. Conclusion: Xuebijing Injection may show a protective effect in patients with severe pneumonia. The mechanism is possibly with the decreased secretion of TNF-α, IL-6, and IL-8.     

Experimental Study on Effect of Angiogenesis with Shenmai Injection

Objective:To study the effect of Shenmai in-jection(SMI)on angiogenesis.Methods:Effect ofSMI on proliferation of fetal bovine serum(FBS)induced bovine aortic endothelial cell(BAECs)and human hepatocarcinoma cell line SMMC-7721     

Effect of Astragalus Injection on Left Ventricular Remodeling in Aged Patients with Acute Early-stage Myocardial Infarction

Objective: To observe the effect of Astragalus Injection (AI) on left ventricular remodeling in aged patients with acute myocardial infarction (AMI). Methods: Patients with AMI were randomly divided into the AI group (46 cases) treated with AI and the control group (46 cases) treated conventionally. Left ventricular end-diastolic volume index (LVEDVI), left ventricular end-systolic volume index (LVESVI), anterior endocardial segmental length (ASL) and posterior endocardial segmental length (PSL) were all assessed by echocardiogram after 1 week and 4 weeks treatment. The cardiac systolic and diastolic functions were detected by nuclide gating cardiac blood pool imaging at the 4th week. Results: After four weeks' treatment, no obvious change of LVEDVI, LVESVI and ASL in the AI group was found, but these indexes increased significantly in the control group, with significant difference shown between the two groups (P<0. 05). As compared with the control group, the left ventricular ejection fraction (LVEF),     

A Clinical and Experimental Study on the Protective Effects of Jiangzhi Tongmai Fang on Endothelial Injury

31 cases of atherosclerosis (AS) were treated with Jiang Zhi Tong Mai Fang (降脂通脉方,formula ofJZTMF),and its effect was compared with 30 cases treated with Iovastatin in the control group.Clinically,the JZTMF formula showed an effect of regulating blood lipids,and therefore it was anti-atherosclerotic.The mechanism is,probably,restoration of the function of endothelial cells (EC) byincreasing the synthesis of 6-keto-PGF_(1α) and decreasing the release of endothelin (ET) as evidenced inthe experimental study.