Therapeutic effect of prolonged hyperbaric oxygen in adjuvant arthritis of the rat.

Development of adjuvant disease in the rat is suppressed if hyperbaric oxygen is administered within one day after the inoculation of an oil suspension of Mycobacterium tuberculosis and continued for 16 to 17 days. Although 10 to 40% of the rodents developed mild arthritis after discontinuance of therapy, most remained symptom-free for at least 50 days. Oxygen administered after arthritis is advanced still exerted a significant curative effect. Possible mechanisms underlying the therapeutic action of oxygen are discussed.     

联合高压氧治疗肝硬化门脉高压疗效观察

目的通过观察肝硬化门脉高压食管胃底静脉曲张患者经高压氧治疗后门静脉系统血流动力学和肝功能的变化,尝试探索高压氧在改善肝功能、预防肝硬化食管静脉曲张再出血维持治疗中的作用.方法2003年1月～2004年12月期间,36例肝炎后肝硬化食管静脉中～重度曲张的住院患者,随机分为3组:高压氧组12例,内科常规联合高压氧治疗2周,每日1次,每次2小时;心得安组12例,心得安10mg及单硝酸异山梨酯20mg,分别为每日3次及2次口服,连续2周;对照组12例内科常规治疗2周.运用彩色多普勒血管超声检测3组患者治疗前后门静脉直径(PV)、脾静脉直径(SV)和门静脉平均血流速度(Vmean)及脾脏厚度的变化,并计算门静脉血流量.同步检测3组患者治疗前后肝功能,前白蛋白(PA)、凝血酶原时间(PT)及吲哚氰绿15min潴留率(ICG15)的变化.结果高压氧组与心得安组血流动力学指标均有不同程度改善,门静脉血流量减少,门脉压力降低;而高压氧组肝功能改善明显优于心得安组及对照组.结论高压氧对防治肝硬化门脉高压食管静脉曲张再出血及改善肝功能具有潜在的治疗作用.     

Galanin in adjuvant arthritis in the rat

OBJECTIVE: To study the concentration changes of galanin in the ankles and spinal cord and to detect the distribution of galanin in different tissues in arthritic rats. METHODS: Adjuvant arthritis was induced by intradermal injection of Mycobacterium butyricum in Freund's incomplete adjuvant at the base of the tail. The concentrations of galanin were measured by radioimmunoassay (RIA) and the distributions of galanin were detected by immunoelectron microscopy (iEM). RESULTS: Measurements were taken on Day 28 after injection. RIA results showed that the concentration of galanin was significantly lower in the ankles and spinal cords of rats with adjuvant arthritis compared to controls. Our iEM results showed a heterogeneous distribution of galanin labelling in different cells and tissue compartments. In arthritic rats, we observed decreased galanin labelling in the sciatic nerve and in macrophage-like cells in the synovial membrane and increased labelling in monocyte lineage cells, polymorphonucleated lineage cells in the bone marrow, fibroblasts in the periosteum, osteoclasts and osteocytes, and lower labelling in osteoblasts compared to controls. CONCLUSION: Galanin is involved in the response to inflammation in adjuvant arthritis and might play a role in the regulation of inflammation and nociception. These findings are in accordance with a biological role of galanin in the development of inflammatory arthritis.     

Studies on the effect of low dose methotrexate on rat adjuvant arthritis

Adjuvant arthritis in rats was induced by the intradermal administration of Freund's complete adjuvant. When these immunized rats were treated orally with low doses of methotrexate (150-600 micrograms/kg/week) a statistically significant suppression of paw inflammation was observed. This low dose of methotrexate was comparable to that used in the treatment of human rheumatoid arthritis (RA). Our results are the first demonstration of the efficacy of low dose methotrexate in an animal model of human RA.     

Incadronate在大白鼠佐剂性关节炎中的关节保护作用

目的 研究二膦酸盐对大白鼠佐剂性关节炎的关节炎症、肿胀以及结构破坏的治疗作用。方法 40只雌性Lewis大白鼠随机分为5组，其中4组通过接种乳酪分枝杆菌诱发关节炎。取接种组中3组自接种后15～42d给予incadronate不同剂量每日1次皮下注射。通过观测关节炎表现，并结合放射学和组织学等方法，分析药物的治疗作用。结果 在incadronate的三种治疗剂量中，大剂量组较小剂量组对关节炎症的抑制作用和关节的保护作用更强。组织学显示，经药物治疗，关节软骨及其下面的骨质结构得到保护；破骨细胞数量减少，并呈现出功能不活跃的彤态。结论 Incadronate可抑制大白鼠佐剂性关节炎的关节炎症、肿胀，保护关节软骨及其下面的骨质结构。提示二膦酸盐类药物可作为类风湿关节炎的辅助治疗药物。     

Therapeutic effect of recombinant vaccinia virus expressing the 60-kd heat-shock protein on adjuvant arthritis

Objective. To analyze the effects of recombinant viruses for 2 heat-shock proteins in the treatment of adjuvant arthritis. Methods. Virus vaccinia recombinant for mycobacterial heat-shock protein 65 (hsp65-VV) and human hsp60 (hsp60-VV) were administered to rats during different stages of adjuvant arthritis. Arthritis score and immunity to the recombinant virus were analyzed. Results. When delivered at the pre-arthritis stage, both constructs ameliorated arthritis; greater protection was observed with hsp60-VV. A specific T cell response to the recombinant proteins was detected. Furthermore, hsp60-VV displayed a clear therapeutic effect on established arthritis. Conclusion. Our results suggest novel avenues of therapeutic intervention in autoimmune arthritis associated with immunity to hsp60.     

Cellular adhesion molecules in rat adjuvant arthritis

Objective. To examine adhesion molecule expression during the progression of inflammation in a rheumatoid arthritis model of adjuvant-induced arthritis (AIA) in rats. Methods. Immunohistochemical analysis was used to determine the distribution of the following adhesion molecules: lymphocyte function–associated antigen 1 (LFA-1; CD11a/CD18), Mac-1 and p150/95 (CD11bc/CD18), intercellular adhesion molecule 1 (ICAM-1), and CD44 in tissue sections from the ankle joints of rats with AIA. Control animals and those with AIA were killed at intervals over a 54-day period after injection with mineral oil and Mycobacterium butyricum, respectively. Results. CD44 and LFA-1 were expressed on lymphocytes, macrophages, and synovial (ST) lining cells. CD44 expression on macrophages was found to be increased compared with control animals by day 18, and was significantly increased by day 41. CD44 expression on lymphocytes significantly increased earlier, on days 11–18. Increased LFA-1 expression on macrophages occurred late, on day 41. LFA-1 expression on lymphocytes was significantly increased on days 25, 47, and 54. ST lining cells exhibited two distinct periods of increased expression, one early, on days 11–25 and one later, on days 41–54. CD11b/c was expressed on macrophages and ST lining cells, showing a significant increase on AIA rat ST lining cells compared with control animals on day 4. No differences in ICAM-1 expression on endothelial cells between rats with AIA and controls were found on any of the days examined. Conclusion. CD44 expression is up-regulated on macrophages and lymphocytes during the early development of AIA, while LFA-1 expression is up-regulated later in the development of AIA. The up-regulation of CD44 and LFA-1 at different times in the development of AIA suggests an important role for these adhesion molecules in establishing and sustaining an inflammatory response in the AIA joint.     

高压氧治疗糖尿病足的疗效研究

目的 探讨高压氧治疗糖尿病足的临床疗效.方法 选取100例糖尿病足患者按照区组随机化分为高压氧组,对照组,各50例.对照组给予糖尿病饮食、降血糖、局部创面处理、抗感染等常规治疗,高压氧组在常规治疗基础之上同时予以高压氧治疗,高压氧治疗压力为0.20 ～0.25MPa,患者吸纯氧60 min,每天1次,共6个疗程.观察治疗前、后空腹血糖、血液流变学、患侧下肢动脉血流峰值的变化情况.结果 治疗后,与对照组相比,高压氧组空腹血糖较低[(5.1±0.8)mmol/L vs.(7.2±0.7)mmol/L,P=0.011];血液流变学得到改善(t=2.381 ～3.854,P均＜0.05);患侧下肢动脉血流峰值较低(t=3.169 ～9.899,P均＜0.05);糖尿病足皮损明显改善[(2.11±1.34) cm2vs.(3.65±1.65)Cm2,P=0.021].高压氧组总有效率和治愈率分别为98％和78％;对照组总有效率和治愈率为76％和48％,差异有统计学意义(P＜0.05).结论 高压氧治疗糖尿病足疗效优于常规治疗,是糖尿病足治疗的有效途径.     

Penetration and effect of topically applied dimethylsulfoxide or indomethacin on adjuvant arthritis in the rat

The present study, using ¹⁴C-DMSO, established the systemic and local load and distribution of topically applied DMSO in adjuvant arthritic rats. Under equivalent conditions, the antiinflammatory effects (systemic and local) of topical DMSO treatments were compared with a topical treatment of a control vehicle or of indomethacin, a known effective antiinflammatory agent. No significant systemic or local antiinflammatory effect of topical DMSO was seen in the adjuvant arthritic rats. Indomethacin, applied topically, had a significant systemic antiinflammatory effect; however, no significant local antiinflammatory effect of indomethacin was observed.