荧光分析法测定血清中微量硒

为测定血清中微量硒,较低温度消化后,用2,3-二氨基萘为试剂,以荧光法测定,取得了满意的结果。方法简便快速,结果稳定准确。血清中加入标准硒,8次测定的回收率为96.6％～102.4％,平均值±标准差为99.6±1.8％。     

硫酸亚铁铵光度法测定土槿皮浸取液中鞣质的含量

本文主要研究了在酒石酸钾钠介质中,用硫酸亚铁铵为显色剂光度测定鞣质的条件,拟定了土槿皮浸取液中鞣质含量的测定方法,10毫升95％乙醇对测定结果均无影响,测定结果与高锰酸钾法一致,回收率在98％～104％之间,标准偏差为1.61×10~(-2)变异系数为1.34％,大量样品测试表明,方法简单快速,易于掌握,结果满意。     

以金丝桃甙为标准品测定罗布麻浸膏中的总黄酮

以金丝桃甙为标准品,用722型光栅分光光度计测定罗布麻浸膏中总黄酮的含量。测定结果:新疆制药厂提供的白罗布森样品中水提浸膏总黄酮的含量为2.85％,醇提浸膏7.99％,醇不溶物0.28％;新疆库尔勒甘草厂水提浸膏为4.92％;江苏东台浸膏厂水提浸膏为3.57％,变异系数以3种不同浓度6次测定数值分别为1.09％,0.94％和0.63％,回收率平均值为97.4％。     

高效液相色谱法测定菟丝子中芦丁、槲皮素及山柰酚的含量

目的：测定菟丝子中芦丁、槲皮素和山柰酚的含量。方法：采用高效液相色谱法,梯度洗脱。结果：芦丁的回收率为99．04％,RSD＝1．12％;槲皮素的回收率为101．33％,RSD＝4．02％;山萘酚的回收率为98．68％,RSD＝1．25％。结论：方法准确可靠,可用于菟丝子药材中黄酮类成分的含量测定。     

UF-100全自动尿沉渣分析仪与尿沉渣镜检的对比测定

目的 探讨UF-100全自动尿沉渣分析仪检测尿中有形成分的临床应用价值。方法用UF-100全自动尿沉渣分析仪及常规镜栓分别对530例随机尿液标本进行检测,并比较结果的一致性。结果 UF-100与常规镜栓法相比,检测红细胞阳性符合率为89％,假阳性率为15．12％,假阴性率为11％;测定白细胞阳性符合率为92．31％,假阳性率为8．23％,假阴性率为7．69％;测定上皮细胞阳性率符合率为80．60％,假阳性率为14．78％,假阴性率为19．4％：测定小圆上皮细胞阳性符合率为50％,假阳性率为19．69％,假阴性率为50％;测定管型阳性符合率为66．67％,假阳性率为8．8％。假阴性率为33．3％;测定结晶阳性符合率为68．89％,假阳性率为7．23％,假阴性率为30.11％;测定类酵母菌阳性符合率为57．12％,假阳性率为0．39％,假阴性率为42．86％。结论 UF-100全自动尿沉渣分析仪对尿液中有形成分主要起过筛作用,由于尿液中有形成分复杂,细茵、结晶、类酵母等均会不同程度地影响RBC、WBC等的计数,对UF-100提示项目结晶、类酵母菌、病理管型阳性、细菌计数过高的标本以及肾病病人的标本,应使用显微镜检查法加以分析鉴别,以提高尿液标本的检验质量。     

滷水(鹽滷)中毒的研究(第三及第四部分)(摘要)

为了判定滷水中毒,根据本题研究第一及第二部分资料,并通过滷水中毒前后的家兔,采用其血清、全血以及腐败血液,用比色法测定其中的镁离子含量,进行比较观察,以确定是否滷水中毒,并观察中毒后血清、血液中镁离子的增加情况。经测定结果证明,正常家兔血清中镁离子含量平均为4.07mg％。滷水中毒后血清中镁离子含量,用一般比色测定结果平均为35.4mg％;光电比色测定结果平均为43.5mg％。正常全血中镁离子含量平均为5.58     

原子吸收法测定安神补脑液中砷和铅的含量

目的：建立测试安神补脑液中砷和铅含量的原子吸收方法,并进行方法学验证,确保测试方法结果准确、可靠。方法：采用原子吸收分光光度计测定,检测波长193．7nm;狭缝0．5nm;灯电流2mA。结果：本法测定砷的线性范围为2-20ng／ml,线性良好,相关系数为0．9991（n=5）,精密度和重现性良好,回收率在91．92％-98．51％,RSD为2．9％;铅的线性范围为1-10ng／ml,相关系数为0．9992（n=5）,精密度和重现性良好,回收率在98．39．92％-103．59％,1LSD为2．1％。结论：本方法操作简单、快速,测定效率高,精密度和重现性良好,灵敏度高,测定结果准确、可靠,适用于安神补脑液中砷和铅的含量测定。     

广西不同产地对叶百部的水溶性浸出物、水分及灰分测定

[目的]测定对叶百部的水溶性浸出物、水分、总灰分及酸不溶性灰分的含量.[方法]按《中华人民共和国药典》2010年版一部附录XA中的水溶性浸出物测定法、IX H中水分测定法(烘干法)和附录IX K中的灰分测定法测定.[结果]对叶百部水溶性浸出物含量为53.41％～80.48％,平均值69.61％;水分含量为6.17％～11.25％,平均值为8.41％;总灰分含量为3.61％～10.55％,平均值为6.19％;酸不溶性灰分含量为0.36％～1.85％,平均值为0.84％.[结论]所得结果可为制定该药材质量标准提供科学依据.     

消痔液中补骨脂素的含量测定

本文重点介绍采用紫外分光光度法测定消痔液中补骨脂素的含量。以此做为控制消痔液制剂的含量标准的依据。根据测定结果确定为每100ml消痔液中含补骨脂素为25～35mg。加样回收率为98.09％;SD=1.5537;CV=1.58％。     

石墨炉原子吸收光谱法测定水中微量铝

目的采用石墨炉原子吸收光谱法,加入重铬酸钾基体改进剂,测定水中微量铝。方法通过改进铝的灰化温度、原子化温度以及基体改进剂来测定水中铝的含量。结果本方法检出限为0．85μg/L,相关系数为0．9996;线性范围为0～100μg／L;相对标准偏为1．08％～1．4％,加标回收率为91％-102％。结论显示研究方法干扰少,灵敏度高,精密度和准确度均能满足测定要求,适合水样中铝的测定。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.