ISOLATION OF TREPOrYEMA PALLIDUM FROM THE BLOOD DURING THE PRJMARY INCUBA- TION PERIOD OF HUMAN SYPHILIS

It was once the belief that syphilis remained a localized infection during the period of the primary chancre, and that by excision of the lesion, penetration and generalized dissemination of the infection from the portal of entry could be prevented. Although this cor.ception is. no longer held, the rate at which Treponema patlidum spreads from. the site of inoculation to invade the blood stream in the human subject remains unknown.     

CLINICAL EVALUATION OF FOUR RECOMBINANT TREPONEMA PALLIDUM ANTIGEN-BASED RAPID TESTS IN THE DIAGNOSIS OF SYPHILIS

Objective To assess the sensitivity,specificity,and feasibility of 4 recombinant Treponema pallidum antigen-based rapid tests in the diagnosis of syphilis.Methods A total of 970 outpatients were selected from the Sexually Transmitted Diseases Centre of Peking Union Medical College Hospital.Venous blood was collected and serum was extracted.T.pallidum antibodies in whole blood,anticoagulant whole blood,and serum were detected using 4 recombinant T.pallidum antigen-based rapid tests.T.pallidum haemagglutination test(TPHA) was considered as the gold standard for the detection of T.pallidum specific antibodies in serum.The sensitivities and specificities of four methods were analyzed.Results The sensitivities and specificities of Abbott Determine Syphilis TP test,SD-BIOLINE Syphilis 3.0 test,VISITECT-SYPHILIS test,and Syphicheck-WB test for serum specimens were 100% and 98.9%,95.7% and 98.0%,94.6% and 98.2%,68.1% and 98.9%;for whole blood were 74.1% and 99.5%,87.9% and 99.4%,73.2% and 99.7%,64.7% and 99.7%.The observed sensitivities of the 4 rapid diagnosis tests were not significantly different with TPHA(P>0.05).Conclusions The 4 rapid tests show good performance and characteristics in the diagnosis of syphilis.Furthermore,they are more sensitive for serum specimens than whole blood.     

ISOLATION OF TREPONEMA PALLIDUM FROM GUMMA OF SKELETAL MUSCLE WITH A REPORT OF FOUR CASES OBSERVED IN NORTH CHINA*

Cases of syphilitic gumma of skelctaI muscles are infrequently met with and cliniciains often mistake the disorder for neoplasm. The rarity of such manifestations justifies a report of four cases which we have observed in the Peiping Union Medical College during the past ten years. Furthermore, in one case, we were fortunate enough to isolate Trefonema pallidm in rabbits inoculated with the biopsy material. As far as we can learn from the literature available to us, Treponema pallidum has not been previously isolated from such cases.     

人主动脉内皮细胞的培养及免疫细胞化学研究

人主动脉内皮细胞体外长期培养在国内首次成功。内皮细胞培养10～15代,细胞倍增时间20h～25h,累积倍增数25～30。应用相差显微镜,扫描、透射电镜,Ⅷ因子相关表面抗原的免疫荧光抗体及血管紧张素转换酶的单克隆抗体,对培养的人主动脉内皮细胞进行形态学及免疫细胞化学的观察与研究。证明,长期培养后的多核内皮细胞是具有内皮细胞特点的变形内皮细胞。     

人胎盘线粒体DNA提取方法的探讨

本文介绍一种人有台盘ＤＮＡ的碱变性提取法。并对提取过程中应注意的问题和解决方法进行了分析和讨论。     

人类精浆特异性抗原p30的分离纯化

作者采用CM-Sephadex-C50,Sephadex-G100和DEAE-Sephadex-A50等柱层析方法,从人类精浆中分离纯化精浆特异性抗原p30,经聚丙烯酰胺凝胶电泳、SDS-凝胶电泳与免疫电泳等鉴定证实。     

从器官移植受者外周血分离出人类疱疹病毒6型

从３１例器官移植受者外周血单个核细胞（ＰＢＭＣｓ）中分离人类疤疹病毒６型（ＨＨＶ－６）。结果显示：１０例移植受者病毒分离阳性，９例经单克隆抗体鉴定证实为ＨＨＶ－６。     

红细胞在分离凝集素中的应用

红细胞是吸附凝集素的天然载体,利用红细胞分离凝集素是一种经济可行的方法。本文在探讨红细胞对植物凝集素吸附和解吸附的基础上,成功地从人胎盘组织中分离到了乳糖专一性凝集素。     

人胎盘绒毛叶神经生长因子纯化与活性鉴定的初步研究

人胎盘绒毛叶匀浆上清,在不同的pH值情况下,依次通过两个离子交换纤维素层析柱和SephadexG-100柱,分离纯化了神经生长因子(NGF)。其分子量约14500,3种主要组分的等电点分别为9.15、9.45和9.65。一个胎盘绒毛叶可获得1.0μg NGF提取物。用血浆凝血块法测NGF提取物的活性表明:纯化后的NGF比活力显著提高。     

三种提纯人外周血中T淋巴细胞的方法对比研究—纯度和收获率的分析

本文报道了用AET-E花环法,常规E花环法、尼龙毛过柱法提纯人外周血中T淋巴细胞的对比研究的结果,最终认定AET-E花环法是收获率高,纯度好的方法,并获得了一次免疫接种必需数量(1×10~7以上)的高纯度(95%左右)的T淋巴细胞免疫原,并对这几种方法的优缺点做了讨论。     

孕妇血清中早孕因子的纯化及其部分特性研究

采用DEAE纤维素(Whatman产)柱层析Sephadex G-100凝胶过滤、聚丙烯酰胺凝胶圆盘电泳(Disc-PAGE)、SDS-聚丙烯酰胺凝胶平板电泳(SDS-PAGE)对50例妊娠3～8周妇女混合血清中早孕因子(EPF)进行纯化提取及部分特性研究,并采用花结抑制试验(Ea-RIT)检测EPF活性,结果表明:(1)EPF存在于早孕妇女血清中;(2)早孕血清中EPF的活性形式主要为21.2KD、26.3KD的蛋白多肽;(3)EPF有明显的免疫抑制活性。     

人脐血间充质干细胞鉴定及用5-氮胞苷诱导后的生物学特性研究

目的探讨脐血间充质干细胞经体外扩增纯化表面CD标志的鉴定及在体外定向分化成心肌样细胞的诱导剂5一氮胞苷（5-aza）作用后的生物学特性。方珐健康妊娠产妇分娩的胎儿脐带血,分离单个核细胞进行培养、传代,取第3代以后细胞用流式细胞仪直接标记分析CD34、CD44、CD90细胞表面标志。10μmmol／L5-aza诱导4周后,进行透视电镜观察,并检测细胞肌球蛋白重链基因的表达。结果体外纯化扩增后的脐血间充质干细胞含有与骨髓来源的间充质干细胞相同的细胞表面标志,第3代以后细胞纯度较高,经5-aza体外诱导培养可形成心肌样细胞。结论脐血间充质干细胞经体外纯化扩增,第3代以后的细胞纯度较高,在体外经5-aza诱导定向分化为心肌样细胞后,其基本的生物学特性未发生改变。     

从人末梢血T细胞cDNA文库中筛选表达人白细胞介素4基因的克隆

白细胞介素4具有多种生物功能,是调节免疫应答的重要淋巴因子之一。本文从以表达型质粒pcD为载体的人末梢血T细胞cDNA文库中,筛选出人IL-4cDNA克隆pcD-X-IL-4。经限制性内切酶酶切及Southern杂交鉴定,结果表明,pcD-X-IL-4包含有人IL-4基因的片段;转染真核COS7细胞后,在细胞培养上清测出T细胞生长因子样活性,说明所含IL-4基因可表达人IL-4完整分子。     

颈椎骨折、脱位手术后并发肺栓塞死亡（附2例报告及文献复习）

目的：加强对颈椎骨折、脱位手术后并发肺栓塞的认识，增强对肺栓塞预防的重视。方法：对我科2例颈椎骨折、脱位手术后并发肺栓塞死亡病例分析。结果：本症通常发生于高能量损伤后的患者，肺血管摄影被认为是诊断的金标准，目前尚无有效救冶方法，其预防措施包括低分子肝素钠的应用和间歇性气压疗法（IPC）。结论：颈椎骨折、脱位手术后并发肺栓塞的原因与多因素相关，其早期诊断比较困难，关键在预防。     

人与大鼠补体C5b—9复合物的提取及相应抗体的制备

目的 提取人及大鼠血清补体Ｃ５ｂ－ｐ复合物并制备抗体。方法 利用兔红细胞能直接活化人补体旁路途径的特性首先获得人或大鼠补体攻击破裂的兔红细胞膜,然后用脱氧胆酸钠溶解后的经超速离心提取Ｃ５ｂ－９复合物。结果 纯化的Ｃ５ｂ－９复合物电镜负染观察有其独特的形态。