带状疱疹患者外周血T淋巴细胞亚群及NK细胞分析

对５０例带状疱疹患者外周血Ｔ淋巴细胞亚群及自然杀伤细胞（ＮＫ）用流式细胞仪进行检测,并与３０例正常人对照,结果带状疱疹组ＣＤ３下降不明显（Ｐ〉０．０５）,ＣＤ４下降明显（Ｐ〈０．０１）,ＣＤ８明显升高（Ｐ〈０．０１）,ＣＤ４／ＣＤ８下降明显（Ｐ〈０．０１）,ＮＫ细胞升高（Ｐ〈０．０１）,提示患者辅助性Ｔ细胞减少,抑制性Ｔ细胞增另,存在免疫抑制现象。     

带状疱疹患者T细胞亚群及相关细胞因子的检测

目的 了解T细胞免疫在带状疱疹发病中的作用,探讨带状疱疹发生的免疫学机制.方法 用流式细胞仪技术和酶联免疫吸附法分别对病例组与对照组T细胞亚群表面标志CD3、C1M和CD8的表达情况.血清Th免疫相关因子白介素(IL)-2、IL-4和肿瘤坏死因子(TNF)-α水平进行分析.结果 带状疱疹患者CD4比例显著降低,CD3比例亦降低,CD8比例升高,CD4/CD8比值下降;血清IL-2水平明显下降,TNF-α水平下降,IL-4水平明显升高.结论 带状疱疹患者体内存在T细胞免疲功能异常.尤其CD4+T细胞的比例降低可能是疾病发生、发展的主要免疫学改变.     

带状疱疹患者细胞免疫功能的检测及意义

带状疱疹是一种常见的病毒性皮肤病。许多资料表明其与细胞免疫功能紊乱有关1,2。笔者通过测定带状疱疹患者的外周血T淋巴细胞亚群,试图对带状疱疹患者的免疫状态作进一步的探讨。临床资料实验组50例患者均为2001年1月~2003年12月我科住院或门诊患者。其中男23例,女27例,年龄22     

带状疱疹患者T细胞亚群的检测

目的:检测带状疱疹患者外周血T细胞亚群表达水平。方法:采用流式细胞仪检测116例带状疱疹患者及80名健康对照者外周血T细胞亚群。结果:带状疱疹患者外周血CD3~+T细胞、CD4~+T细胞均显著高于健康人群,患者不同年龄组间、不同病程组间CD3~+T细胞、CD4~+T细胞无明显差异。结论:带状疱疹患者CD3~+T细胞、CD4~+T细胞升高,提示特异性细胞免疫得以重建。     

带状疱疹患者外周血T淋巴细胞亚群及NK细胞的测定

为了探讨带状疱疹(HZ)患者的细胞免疫功能,对36例急性期HZ患者的外周血T淋巴细胞亚群及自然杀伤细胞(NK)进行了测定,现将结果报告如下。资料与方法1.病例选择　选择本院门诊未经治疗的急性期HZ初诊患者36例,其中男22例,女14例;年龄19～82岁,平均55.44岁。所有病例为寻常性,均有     

白癜风患者外周血T细胞亚群的检测

目的了解各型期白癜风患者的细胞免疫功能状态。方法采用链菌素亲生物素-碱性磷酸酶链接法(SAP法)检测125例白癜风患者外周血T淋巴细胞亚群,同时以50例健康体检者做对照。结果寻常型进展期患者CD3,CD4水平低于正常对照组,CD8则升高(P<0.01);稳定期泛发性及限局性CD8升高,CD4/CD8比值下降(P<0.05);散发性CD4下降(P<0.01);节段型及肢端性各项检测结果无统计学差异。病期在1年以内的寻常型患者CD3,CD4低于病期超过1年者(P<0.01)。结论各型期白癜风患者存在不同程度的细胞免疫功能异常。     

带状疱疹患者细胞介导免疫激活状态研究

带状疱疹患者细胞介导免疫激活状态研究劳力民孙国均张行杨骅细胞介导免疫（ＣＭＩ）在水痘－带状疱疹病毒（ＶＺＶ）感染恢复过程中起了重要作用［１］，但其间机理尚不十分清楚。我们测定了３０例带状疱疹患者血清新喋呤水平作为单核细胞／巨噬细胞系统激活指标，血清白...     

带状疱疹患者外周血CD+4T细胞表面CD28的表达

目的 研究带状疱疹患者外周血CD3+T、CD4+T及CD4+T细胞表面CD28的表达,探讨CD4+T细胞活化功能状态在带状疱疹发病中的意义.方法 利用流式细胞仪检测35例带状疱疹患者外周血CD3+T、CD4+T及CD4+T细胞表面CD28的表达,以20例正常人作为对照,并分别与患者的临床特征进行比较.结果 状疱疹患者外周血CD3+T细胞、CD4+T细胞及CD4+CD28+T细胞的比率较正常对照组显著降低;不同年龄组和不同病程组患者外周血CD4+T细胞和CD4+CD4+T细胞的比率差异有统计学意义;但不同病情组的表达差异无统计学意义.结论 状疱疹患者外周血CD4+T细胞表面的CD28表达下调,通过影响CD4+T细胞活化,可能参与带状疱疹的发生.     

带状疱疹患者细胞免疫功能的测定

带状疱疹是由水痘带状疱疹病毒（ＶＺＶ）感染引起的疱疹性疾病。其发病与机体的免疫状态特别是细胞免疫功能降低有关［１］。为了更准确地了解带状疱疹患者细胞免疫功能的变化,我们对３０例带状疱疹患者外周血Ｔ淋巴细胞亚群、自然杀伤细胞（ＮＫ）、白细胞介素２受...     

Relationship between prior knowledge about herpes zoster and the period from onset of the eruption to consultation in patients with herpes zoster

Herpes zoster (HZ) is a common internal infection caused by latent varicella zoster virus. Emergence of antiviral chemotherapy has changed the treatment of HZ dramatically, but the effects of such therapy are documented only in patients who started treatment within 72 h of HZ onset of the eruption. There have been few studies addressing the question of factors that determine early attendance of patients at a clinic. We questioned 256 patients with acute HZ about: (i) date from onset of the eruption to first clinic visit; and (ii) their prior knowledge of HZ. We found a tendency that patients who already knew about HZ had consulted dermatology clinics earlier (P < 0.05). People most commonly obtained information about the disease from friends and family members who had previously had HZ, but not from the Internet or other mass media. Our results indicate that patient education is important for early attendance at dermatology clinics, which in turn, should result in the improved outcome of antiviral chemotherapy and prevention of postherpetic neuralgia.     

带状疱疹309例临床分析

目的：探讨带状疱疹（Hz）的发病特点、治疗与疱疹后遗神经痛（PHN）的相关性。方法：回顾性分析309例患者的一般资料、临床表现、实验室检查及临床转归。结果：病程及PHN的发生与患者年龄、初诊时间、治疗用药相关。结论：早期治疗、合理用药对缩短病程及防止PHN发生起一定的作用。     

老年带状疱疹60例临床分析

目的 探讨老年带状疱疹的临床特点。方法 选择近6年来我院皮肤科门诊老年带状疱疹60例，中青年带状疱疹81例，就其前驱症状、皮疹分布、后遗神经痛及疗效方面进行比较。结果 老年组有前驱症状者明显高于中青年组（P〈0.01），出现三叉神经受累（P〈0．05）、血疱大疱（P〈0．01）及后遗神经痛（P〈0．01）的几率增高。结论 老年人带状疱疹要早诊断，早治疗，对无激素禁忌证者，应早期适量使用激素。     

无环鸟苷及干扰素治疗单纯疱疹和带状疱疹的疗效观察

47例单纯疱疹及带状疱疹病人随机分为无环鸟苷治疗组(12例),干扰素治疗组(21例)及对照治疗组(14例)。三组在年龄及发病时间上经统计学处理无显著性差异(P>0.05)。结果表明:每日肌注5万单位干扰素比外用3％无环鸟苷软膏(或0.1％无环鸟苷药水)和对照治疗组,能明显缩短病程,减轻症状(P<0.001)。     

Atypical herpes zoster in a patient with AIDS

Objective To report a case of persistent infection by varicella-zoster virus (VZV) in an HIV-infected patient who presented with atypical, hyperkeratotic viral lesions and a partial response to acyclovir. Methods Viral changes found on histopathological examination; definitive diagnosis of VZV infection was established by viral culture. Results Lesions of chronic hyperkeratotic herpes zoster responded partially to acyclovir therapy, and complete resolution was achieved with intravenous foscarnet. Absence of visceral involvement. Conclusion Prolonged acyclovir therapy for herpes virus infections in HIV-seropositive patients may represent an important pathogenic factor for the appearance of atypical clinical forms associated with drug-resistant viral strains.     

45例带状疱疹并脑膜脑炎的回顾性研究

目的：探讨带状疱疹并发脑膜脑炎的相关因素及发病机制。方法：汇集了山东省12家省市级大医院皮肤科及神经内科住院患者的资料，共有45例，从临床及实验室等方面进行了总结分析。结果：认为年龄不是主要因素，各种原因导致的免疫抑制是重要因素，颅神经尤其是三叉神经眼支受累引起脑炎占首位，其次是肋间神经。结论：带状疱疹并发脑膜脑炎的机制是由于VZV直接侵入脑组织，也存在免疫机制，因此在治疗中要合理的运用抗疱疹病毒药物、糖皮质激素及对症治疗，一般预后还是好的。     

头面部带状疱疹患者的临床与肌电图分析

目的 :　探讨头面部带状疱疹患者的临床表现与不同年龄组肌电图特征。方法 :　对门诊确诊的 6 8例头面部带状疱疹患者按不同年龄段的临床表现与肌电图特征进行分析。结果 :　患者发病前诱因以发热、感冒、过度劳累为多 (6 4 % ) ;发病以先有神经痛而后发疹者为最多见 (72 % ) ;在肌电图检测中 ,瞬目反射异常阳性率最高 (5 9% ) ,其次为异常自发活动 (37% )、混合肌肉动作电位 (CMAP)异常 (2 1% )、MCV异常 (12 % ) ;在上述四项检测中 ,除CMAP异常中各年龄组均无显著性差异 (P >0 .0 5 )外 ,其余三项中均以 >6 0岁年龄组最高 ,4 1～ 6 0岁组次之 ,≤ 4 0岁组最低 ,其间的差异均有显著性意义 (P <0 .0 1)。结论 :　肌电图是检查头面部带状疱疹神经肌肉是否受累的主要手段 ,其中以瞬目反射异常的阳性率最高。     

Study on the T-helper cell 1/2 cytokine profile in blister fluid of patients with herpes zoster and its clinical significance

Herpes zoster (HZ) is a Varicella zoster virus infection disease. Previous studies have presumed the connection between development of HZ and involvement of cellular immunity in peripheral blood. However, whether cellular immunity plays a role in the local skin lesion has not been addressed. To explore the levels of T-helper cell (Th)1/Th2 type cytokine profiles in the blister fluid of the skin lesions from the patients with HZ and its role in pathogenesis, we used the cytometric bead array kit to compare the levels of cytokines (interleukin [IL]-2, tumor necrosis factor [TNF]-α, IL-10 and IL-4) in blister fluid from 46 patients with those from the suction blister fluids from 20 volunteers without any infectious disease (the control group). The results indicated that the levels of Th1 cytokines, IL-2 and TNF-α in the blister fluid from the patients' skin lesions were significantly lower than those from the control group, whereas the levels of Th2 cytokines IL-10 and IL-4 were significantly higher than those in the control group. Moreover, significant variation of the levels of Th1/Th2 cytokines (IL-2, TNF-α, IL-10 and IL-4) in the blister fluid from the HZ patients' lesions was also observed among different stages of the disease. It is concluded that a cytokine imbalance was present in the local lesions of patients with HZ during disease development. Our data suggested that the Th immunity was associated with disease activity, which may play an important role in the pathogenesis of HZ.     

Incidence of serum antibody titers against varicella zoster virus in Japanese patients with herpes zoster

Herpes zoster is an internal reactivation of varicella zoster virus following establishment of latent infection in the dorsal root ganglia during primary infection, which presents as chickenpox. Therefore, serologically, herpes zoster patients already have anti‐varicella zoster virus immunoglobulin G at the onset of disease. Hence, positive serum antibody does not confirm the diagnosis of herpes zoster. We retrospectively investigated the incidence of varicella zoster virus‐specific complement fixation in 865 zoster patients at initial presentation to a dermatology clinic. As a result, 66% of patients showed negative complement fixation, with patient numbers decreasing as titer increased. Paired complement fixation tests conducted within a short period showed a marked elevation in titer, and complement fixation titer gradually decreased after a year. Furthermore, incidence showed no correlation with patient age. These observations indicate that the complement fixation titer at first visit is mainly influenced by the duration from onset to presentation at clinic. Our findings indicate that a positive complement fixation result by single‐point testing confirms at least recent onset of herpes zoster, while paired tests can confirm disease when primary tests are negative.