傣药比比娘中白花丹醌的分离

从傣药比比娘的根中分离得到一个单体,经初步鉴定为白花丹醌,文献报道白花丹醌有抗菌祛痰等作用。     

RP-HPLC测定不同产地白花丹中白花丹醌的含量

 目的分析不同产地白花丹药材中白花丹醌含量的差异。方法采用Kromasil C₁₈柱(4.6mm×200mm,5μm),柱温:30℃,流动相:甲醇-水(65∶35),检测波长:213nm,流速:1mL·min^-1。结果白花丹醌在0.0104～0.3328μg,峰面积与进样量具有良好的线性关系,回归方程为Y=-22.32+9527.9X,r=0.9999,平均回收率为98.7%。结论本法简便、准确、重复性好,可用于控制白花丹药材质量。     

RP-HPLC测定民族药材白花丹不同药用部位中白花丹醌的含量

目的：建立白花丹药材中白花丹醌的含量测定方法并考察白花丹不同药用部位中的含量。方法：色谱条件：Kromasil C₁₈柱(4.6 mm×200 mm,5 μm),柱温30 ℃。流动相甲醇-水（65∶35）,检测波长213 nm,流速1 mL·min^-1。结果：白花丹醌在0.020 8～0.104 μg,峰面积与进样量具有良好的线性关系,回归方程Y=-14.29+9 138.9 X,r= 0.999 9,平均回收率为98.7%。白花丹各部位中白花丹醌的含量分别为：根0.394%,茎0.050%,叶0.031%。结论：本法简便、准确、重复性好,可用于控制白花丹药材质量。     

HPLC同时测定白花丹中白花丹醌及香草酸的含量

目的:建立同时测定白花丹中白花丹醌和香草酸含量的方法,为药材的质量评价提供科学依据。方法:采用高效液相色谱法,以甲醇-0.15%磷酸为流动相进行梯度洗脱,检测波长为260 nm,流速为1.0 ml/min。结果:白花丹醌在9.95~199μg范围内与峰面积值呈现良好的线性关系,r=0.9998,平均回收率为99.52%;香草酸在10.1~202μg范围内与峰面积值呈现良好的线性关系,r=0.9997,平均回收率为99.58%;白花丹中白花丹醌平均含量为0.0310%,香草酸平均含量为0.0124%。结论:本法简便、准确,灵敏度高,重复性好,可用于控制白花丹药材的质量。     

瑶药猛老虎不同药用部位中白花丹醌的含量测定

目的：测定瑶药猛老虎不同药用部位中白花丹醌含量。方法：采用HPLC法测定,色谱柱为依利特Hypersil ODS（4．6mm×250mm,μm）,流动相为甲醇-水（65：35）,检测波长213nm。结果：白花丹醌在O．01～0．32μg范围内具有良好的线性关系,回归方程为Y=18211600X＋10434．57758,r=0．99946（n=6）,平均加样回收率101．7％,RSD=2．43％。结论：猛老虎根中白花丹醌含量最高为0．324％。所用方法重现性好,结果准确可靠。     

HPLC测定不同采收途径及不同部位白花丹中白花丹醌的含量

目的:分析不同采收途径及不同部位的白花丹中白花丹醌含量,寻找富集白花丹醌的药用部位和白花丹的最佳采收途径。方法:采用HPLC法测定不同采收途径白花丹和不同部位中白花丹醌的含量:色谱柱为Hypersil ODS(25cm×4.6mm,25μm),流动相为甲醇-水(65∶35),流速为1.0mL/min,检测波长为213nm,柱温30℃。结果:白花丹醌在0.01μg～0.32μg含量范围呈线性关系,Y=18211600X+10434.57758,r=0.99946,RSD=1.28%(n=6);新鲜的白花丹根部、茎部及叶中白花丹醌的含量分别为0.026%、0.006%和0.014%;干燥的白花丹根部、茎部及叶中白花丹醌的含量分别为0.324%、0.082%和0.174%;枯萎的白花丹茎部、叶及穗中白花丹醌的含量分别为0.002%、0.001%和0.003%。结论:干燥组白花丹中白花丹醌含量高于新鲜组和枯萎组,白花丹根部的白花丹醌含量最高,其次是穗,叶、茎。本法简便、准确,灵敏度高,重复性好,可用于控制白花丹药材的质量。     

白花丹醌的研究进展

近年来天然药物及其提取物在预防和治疗方面的作用引起了医学界研究者的广泛关注,白花丹的提取物——白花丹醌在治疗肝纤维化方面及其他领域成为研究者的首选之一。最近的研究表明白花丹醌在癌症治疗方面是理想的重药,鉴于其用途极大,为了进一步临床前制剂开发研究,就白花丹醌的提取、药理作用以及综合利用作如下述说。     

白花丹醌的研究进展

近年来天然药物及其提取物在预防和治疗方面的作用引起了医学界研究者的广泛关注,白花丹的提取物——白花丹醌在治疗肝纤维化方面及其他领域成为研究者的首选之一。最近的研究表明白花丹醌在癌症治疗方面是理想的重药,鉴于其用途极大,为了进一步临床前制剂开发研究,就白花丹醌的提取、药理作用以及综合利用作如下述说。     

不同栽培条件下白花丹根、茎、叶中白花丹醌的含量测定

 目的 对家种不同栽培条件下白花丹药材根、茎、叶中的白花丹醌进行含量测定,为建立白花丹野生变家种的栽培技术规范提供实验数据。方法 采用高效液相色谱法测定家种药材根、茎、叶中白花丹醌的含量。结果 在熟土和黏土中生长的药材中白花丹醌的含量高于沙土、生土;施加K肥和N肥的药材中白花丹醌的含量高于施加P肥的药材,在全光照条件下生长的药材白花丹醌含量最高;不同繁殖方式中,根、茎和叶中白花丹醌的含量变化不大。结论 课题组前期研究成果表明,白花丹醌为白花丹药材抗癌和抗炎等生物活性的主要有效成分,因此,从白花丹醌含量的角度考虑,白花丹药材的最佳栽培条件为：土壤为黏土,采用扦插苗,生长过程中施加一定的N肥和K肥,光照条件为全光照,在此条件下可获得优质高产的白花丹药材。     

傣药比比蒿(白花丹)的研究概况

本文较为全面的介绍了傣药比比蒿在傣医和中医中的临床应用,并阐述了它的化学成分、药理作用和开发前景。     

白花丹化学成分的研究

目的:研究白花丹Plumbago zeylanicaLinn.地上部分的化学成分。方法:对白花丹95%乙醇提取物的乙酸乙酯部分进行色谱分离,通过理化性质和波谱分析鉴定化合物的结构。结果:从白花丹中分离得到9个化合物,分别鉴定为白花丹醌(Ⅰ),isoshinanolone(Ⅱ),白花丹酸(Ⅲ),β-谷甾醇(Ⅳ),对羟基苯甲醛(Ⅴ),反式桂皮酸(Ⅵ),香兰子酸(Ⅶ),2,5-二甲基-7-羟基-色原酮(Ⅷ),3-吲哚甲醛(Ⅸ)。结论:化合物Ⅴ,Ⅶ,Ⅷ和Ⅸ为首次从白花丹属植物中分离得到。     

RP—HPLC测定海南产胡椒根中胡椒碱的含量

目的：采用RP—HPLC首次建立了海南产胡椒根中胡椒碱的含量测定方法。方法：分析柱采用Eclipse XDB-C18（4．6＊ 150mm,5um）色谱柱,流动相为甲醇-水（V：V=75：25）;流速：1．0mL／min;检测波长：343nm。结果：胡椒碱的线性范围分别为0．1～1．0mg／ml;相关系数为0．9997。平均加样回收率分别为9．4％,98．7％,100．9％;RSD为2．1％,1．6％,2．3％（n=9）。结论：采用RP—HPLC同时测定海南产胡椒根中胡椒碱的含量,该法简单、可靠,可作为控制胡椒根药材质量的参考标准。     

HPLC法测定马先蒿属三种药用植物中桃叶珊瑚苷的含量

目的 建立马先蒿属3种植物中桃叶珊瑚苷的测定方法。方法 药材用乙醚脱脂后，甲醇提取，Varian C18柱；流动相：甲醇-水(15：85)；检测波长：204nm；流速：0．5mL／min。结果 桃叶珊瑚苷在太白参、扭盔马先蒿、短茎马先蒿中的含量分别为0．90％，1．94％，0．95％；回收率分别为102．3％，98．2％，97．6％。结论 马先蒿属植物具有较好的药用开发前景，并为其质量评价提供参考。     

反相高效液相色谱法测定新疆不同产地大蒜中蒜氨酸的含量

目的建立高效液相色谱测定大蒜中蒜氨酸含量的方法。方法采用Z irchrom ODS柱,以甲醇-水(3∶7)为流动相,流速0.5 m l.m in-1,检测波长为214 nm。结果蒜氨酸在2.26~22.6μg呈良好的线性关系,相关系数r=0.999 1,方法灵敏、准确、重复性好,平均回收率98.7%。日内精密度RSD为1.3%,日间精密度RSD为3.4%。结论实验表明该法简便,快速,适用性好。     

Evaluation of anti-inflammatory potential of Pavetta indica Linn. leaf extract (family: Rubiaceae) in rats

The anti-inflammatory potential of methanol extract of Pavetta indica Linn. leaves (Family: Rubiaceae) was evaluated against several models of inflammation such as carragenin, histamine and dextran induced pedal inflammation in rats. The extract showed 48.41%, 41.10% and 24.22% inhibition respectively; when compared to that of control animals. The effect was comparable with that of the standard drug indomethacin, a standard non-steroidal anti-inflammatory drug. Simultaneous subplantar administration of the extract and carrageenin in a mixture helps in differentiating true anti-inflammatory action from an apparent anti-inflammatory effect due to counter-irritant activity. The methanol extract also effectively and significantly reduced cotton pellet induced granuloma. The percentage of inhibition was 62.78 at the dose 500 mg/kg, thereby suggesting its activity in the proliferative phase of the inflammatory process.     

薄层扫描法测定人体血液中牛磺酸的含量

目的：对人血中牛磺酸的含量测定研究,方法：用薄层扫描法,结果：方法简便、精密度高、重视性好,灵敏度可达40ng,平均回收率为98．97％,结论：适合大批量样品分析,可用作体内药代动力学研究中牛磺酸的检测。     

Central nervous system stimulatory action from the root extract of Plumbago zeylanica in rats

The effects of a 50% ethanol extract of the root of Plumbago zeylanica (P. zeylanica) were investigated on locomotor behaviour and central dopaminergic activity in rats. The effects on the ambulatory behaviour were assessed along with the levels of dopamine (DA) and its metabolite homovanillic acid (HVA) in the striatum after a single oral dose (100, 200 and 300 mg/kg body weight) of the extract. The extract significantly increased the spontaneous motility in animals. The ambulatory and rotatory behaviour in the treated groups were higher than in the control group (p < 0.05). There were marked differences in the ambulatory behaviour between 100 and 300 mg/kg, indicating that the responses were stimulatory and dose-dependent. The stereotypic behaviour which is characteristic of a dopamine agonist showed biphasic effects. However, there was no significant difference between the groups (p > 0.05). The results showed that the extract of the root of P. zeylanica specifically enhanced the spontaneous ambulatory activity without inducing stereotypic behaviour. The neurochemical estimations revealed elevated levels of DA and HVA in striatum compared with the control rats (p < 0.01). The levels were higher for the 100 mg/kg treated group than the other groups. The levels declined by increasing the dosage of the extract to 200 mg/kg and 300 mg/kg, however, these levels remained higher than the control group. The relationship between motor activity and levels of dopamine are not parallel. These behavioural and biochemical results indicated stimulatory properties of the extract of the root of P. zeylanica, which may be mediated by dopaminergic mechanisms in the rat brain.     

An investigation into the biochemical basis of the observed hyperglycaemia in rats treated with ethanol root extract of Plumbago zeylanica

The effects of the ethanol extract of the root of Plumbago zeylanica on key enzymes of glycolysis and other biochemical parameters were studied in the rat. The results show that thigh muscle hexokinase, phosphofructokinase, pyruvate kinase and lactate dehydrogenase activities were significantly reduced (p < 0.05) by 12.07%, 51.02%, 24.32% and 25.16% respectively in rats treated with the ethanol extract of Plumbago zeylanica when compared with the controls. Serum pyruvate and lactate were significantly lowered in the experimental rats by 23.64% and 46.29%, respectively. The difference between the supernatant protein means was not statistically different (p > 0.05) suggesting the preservation of protein synthesis in the muscle of the extract‐treated rats. The reduction in the activities of the key enzymes of glycolysis and its end‐products suggests a reduction in flux across the glycolytic pathway in the extract‐treated rats. This may be a result of impaired delivery to, and utilization of, glucose by the peripheral tissue, thus substantiating the reported hyperglycaemia in the extract‐treated rats. Copyright © 1999 John Wiley & Sons, Ltd.