HPLC法测定复方益肾胶囊中淫羊藿苷的含量

目的：建立复方益肾胶囊中淫羊藿苷的含量测定方法,为复方益肾胶囊质量标准研究提供依据。方法：采用高效液相色谱法测定中药复方益肾胶囊中淫羊藿苷的含量。以Diamnosil C18（4.6 mm×250 mm,5μm）为色谱柱,乙腈-0.1%磷酸（25∶75）为流动相;检测波长为270 nm。结果：此方法线性良好,平均加样回收率为98.7%。结论：本方法精密度高,分离度良好,可用于中药复方益肾胶囊中淫羊藿苷的含量测定。     

HPLC法测定前列舒乐胶囊中淫羊藿苷的含量

目的:建立前列舒乐胶囊中淫羊藿苷含量测定方法.方法:采用高效注相色谱法,色谱柱为(Dia-monsil(TM)C18柱,流动相为乙腈-水(30:70);检测波长为270nm.结果:淫羊藿苷的线性范围为0.307～1.53μg,r=0.9999,回收率为99.22%,RSD(%)=1.12%(n=6).结论:本测定方法简便、准确、重复性好,可用于测定前列舒乐胶囊中淫羊藿苷的含量.     

HPLC测定前列舒乐胶囊中淫羊藿苷的含量

目的 建立HPLC测定淫羊藿中淫羊藿苷含量的方法.方法 采用BDS HYPERSIL C18色谱柱(4.6mm×25mm,5 μm);流动相:乙腈-水(30:70);检测波长:270nm;流速:1.0mL/min.结果 淫羊藿苷在0.506～2.024μg范围呈良好的线性关系,r=0.9996(n=5),平均回收率99.4%,RSD=1.08%(n=5).结论 该方法简便、准确、重现性好,可作为控制前列舒乐胶囊质量的方法.     

炙淫羊藿饮片稳定性初步研究

目的?考察炙淫羊藿饮片的稳定性。方法?对炙淫羊藿饮片中淫羊藿苷、宝藿苷I进行薄层色谱鉴别、水分、灰分以及酸不溶性灰分测定;以紫外分光光度法测定炙淫羊藿饮片总黄酮含量,以HPLC测定淫羊藿苷、宝藿苷I,考察炙淫羊藿饮片稳定性。结果? 室温留样和加速实验考核项目均符合要求。结论?炙淫羊藿饮片稳定性良好;高效液相色谱法测定炙淫羊藿中淫羊藿苷及宝霍苷I准确可靠,为炙淫羊藿的质量控制提供了科学依据。      

HPLC测定补肾壮骨颗粒中淫羊藿苷的含量

目的:补肾壮骨颗粒为我院院内制剂,淫羊藿是方中君药.文中采用HPLC建立补肾壮骨颗粒中淫羊藿苷含量的测定方法. 方法:采用HPLC法,色谱柱:Agilent HC-18(150mm×4.6mm,5μm),柱温:30℃,以乙腈-水(30∶ 70)为流动相,流速为1.0ml/min,检测波长270nm,测定了3批补肾壮骨颗粒中淫羊藿苷的含量. 结果:在HPLC色谱中,淫羊藿苷可与其他成分分离,且在0.0962～0.6734μg范围内与峰面积呈良好的线性关系,r2=1(P<0.001),样品平均回收率为94.4%,RSD为1.83%(n=6). 结论:该法分离效果好,分析快速、准确,灵敏度高、重现性好,可用于补肾壮骨颗粒中淫羊藿苷的含量测定.     

高效液相色谱法测定化瘀通冠颗粒中淫羊藿苷的含量

目的:建立化瘀通冠颗粒中主要有效成分淫羊藿苷的高效液相含量测定方法.方法:采用高效液相色谱法,色谱柱为Kromasil C18柱(4.6 mm×250 mm,5 μm),流动相为乙腈-水(26:74),流速为1.0 ml/min,检测波长为270 nm,柱温为室温.结果:淫羊藿苷进样量在0.019 49～0.389 80 μg范围内线性关系良好(r=0.999 8),平均回收率为100.03%.RSD为0.92%.结论:本方法简便可行、重复性好,可用于本制剂中有效成分淫羊藿苷的含量测定.     

安神补脑胶囊中淫羊藿苷含量测定方法的研究

目的:建立安神补脑胶囊中淫羊藿苷含量的测定方法。方法:采用高效液相色谱法,C_(18)柱(4.6 mm×250 mm,5μm);流动相:乙腈-0.1%三氟乙酸(30∶70);检测波长:270 nm;流速:1.0 m L/min。结果:淫羊藿苷在0.050 6~2.024 0μg范围内有良好的线性关系(r~2=0.999),平均回收率为96.4%,RSD=0.56%。结论:该方法准确、快速、简便,可用于安神补脑胶囊中淫羊藿苷的含量测定。     

HPLC法测定妇宁康胶囊中淫羊藿苷含量

目的:建立妇宁康胶囊中淫羊藿苷的含量测定方法.方法:色谱柱为Diamonisil(TM钻石)C18柱(150mm×4.6mm,5μm),流动相为甲醇一水一冰醋酸(60:40:0.3);检测波长270 nm;流速:1.0ml·min-1.结果:淫羊藿苷进样量在0:0315-0.189μg时呈良好的线性关系(r=0.9995).结论:本法操作简便,重现性良好.     

HPLC法测定二仙增精合剂中淫羊藿苷的含量

目的 建立二仙增精合剂中淫羊藿苷的含量测定方法.方法 采用高效液相色谱色谱法,色谱柱:Sinochrom ODS-bp(4.6 mm×250 mm,5μm);流动相:乙腈-水(体积比26∶74);柱温:30℃;检测波长:269nm;流速:0.9 mL·min-1.结果 淫羊藿苷在0.118～1.18 mg·mL-1之间...     

心叶淫羊藿化学成分研究(Ⅱ)

目的 对心叶淫羊藿 Epimedium brevicornum 全草进行化学成分研究.方法 利用硅胶柱色谱、高效液相色谱分离和纯化,通过理化方法及波谱数据分析鉴定其结构.结果 分离得到8个化合物,波谱鉴定为:5-羟基-2-异戊烯基苯酚-1-O-β-D-吡喃葡萄糖苷(Ⅰ)、4-羟基-2-异戊烯基苯酚-1-O-β-D-葡萄糖苷(Ⅰ)、5,7,4'-三羟基黄酮-7-O-a-L-鼠李糖苷(Ⅱ)、宝藿苷Ⅰ(baohuoside Ⅰ,Ⅳ)、宝藿苷Ⅱ(baohuoside Ⅰ,Ⅴ)、淫羊藿苷C(icariside C,Ⅵ)、淫羊藿苷(icariin,Ⅶ)和淫羊藿苷I(icariside Ⅰ,Ⅷ).结论 化合物Ⅰ为新化合物,命名为淫羊藿酚;化合物Ⅰ和Ⅱ系首次从该植物中分离得到.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.