The Effect of Itraconazole on the Vaginal Candidiasis under Different Immunity Conditions in Mice

To study the effect of itraconazole on the vaginal candidiasis caused by Candida under different immunity conditions, the fungal vaginitis model was established in female ICR mice by in- travaginal inoculation of suspension of C. albicans after the animal had been pretreated with estradiol or dexamethasone. Mice were divided at random into different groups and then treated with itracona- zole or IFN-γ given by gastrogavage. The burden of the fungus in the vaginal lavage fluids in the mice of the different groups was measured dynamically at different time points after the beginning of the drug treatment. The difference in the effect of itraconazole on the vaginal candidiasis between normal immune system group (group A) and control group (group D) was statistically significant (P<0.01). The difference in the efficacy of itraconazole among immunosuppressed group (group E), immuno-regulated group (group F) and the control group (group G) was statistically significant (P<0.01). But on the 5th, 6th, 7th, 9th, 11th day after the inoculation the average level of colony forming unit (CFU) of groups A, E and F showed no statistically significant difference (P>0.05). It is concluded that the efficacy of itraconazole in the treatment of the vaginal candidiasis under different immunity conditions (groups A, E and F) in mice were all good, but there was no difference in the anti-fungal effect of itraconazole among the three groups.     

Expression of IFN-γ and its receptor alpha in the peripheral blood of patients with chronic hepatitis C

Background It has been known that intra-cellualr immunity is important for defense against viral infections and this function lies with interferon gamma (INF-γ). Here we evaluated the role of IFN-γ system in the pathogenesis of chronic hepatitis C (CHC).Methods The levels of interferon gamma receptor alpha (IFNGRα) on the peripheral lymphocyte membrane were assayed with flow cytometry. The plasma concentrations of the cytokines IFN-γ and IL-10 in CHC patients and normal controls were assayed by enzume-linked-immunosorbent assay (ELISA). The samples were collected randomly from Xinjiang Autonomous Region, Zhejiang and the northern regions of Jiangsu Province in China. Results The levels of IFNGRα in CHC patients were significantly lower than that of normal controls (NC), especially among patients during the stable stage (P&lt;0.001), whereas there were no significant differences between CHC in active and stable stages. Among the patients of the three regions, there were no significant differences between patients from Xinjiang and Zhejiang provinces, but both had statistically significant difference compared with the patients from Jiangsu Province (P&lt;0.001). Plasma IFN-γ and IL-10 concentrations in CHC patients decreased significantly, IFN-γ in particular, but there were no significant differences in these levels between various stages of the disease. The IFN-γ/IL-10 (Th1/Th2 ) ratio in patients was reversed. Conclusion There may be defects in the IFN-γ system in chronic HCV infected subjects and a low immune response, which may play an important role in the persistence of HCV infection.     

Effect of NS398 on inducing apoptosis and down-regulating Bcl-2 protein in human osteosarcoma cell MG-63 line

Objective： This study investigated the effect of NS398 on anti-proliferation and the mechanism of inducing apoptosis of human osteosarcoma cell MG-63 line in vitro. Methods： Growth suppression was detected by MTT method. Special morphological changes of apoptosis were observed by transmission electron microscopy （TEM） and DNA fragments electrophoresis. The apoptotic rates were quantified by flow cytometry （FCM）. And Bcl-2 protein level were detected by Western Blotting assay. Results： Different concentration NS398 inhibited the cell growth. Through TEM and DNA electrophoresis, the special morphological changes were observed after 24, 48 and 72 h treatment with NS398. The apoptotic rates of MG-63 cells treated with NS398 were respectively, significantly higher than that of the control group（ P 〈0.01 ）. Western blot assay showed that NS398 reduced Bcl-2 protein expression. Conclusion： NS398 suppressed the proliferation of human osteosarcoma cell MG-63 line and induced apoptosis. The mechanism may be associated with down-regulation expression level of bcl-2 protein.     

Effects of Concurrent Use of rh-IFN-γ and Curcumin on the Anti-proliferative Capacity of HL-60 Cells

Cellproliferationisacomplexprocessinvolvingtheregulatedexpressionofmulti-geneproducts.Thereisgrowingevidencetosupporttheinvolvementofparacrinegrowthhormoneintheregulationofcellgrowth.Thetypicalofthesemoleculesaresecretoryproteinslikeinterferon(IFNs).Likemostcytokines,IFNsmustbindtospecifichighaffinitycellularreceptorstoexertbiologicaleffects.Theseeffectsin-cluderegulationofexpressionofspecificgenes,antiviralproperties,andinhibitionofcellgrowthandproliferation["2J.Re-cently,wereportedthatan…     

The expression of PPARγ in reflux esophagitis, Barrett esophagus and esophageal adenocarcinoma

目的 观察反流性食管炎、Barrett食管和食管腺癌中食管上皮细胞过氧化物酶体增殖物激活受体γ(PPARγ)的表达及细胞增殖的变化特点.方法 胃镜下取20例反流性食管炎(RE组)、20例Barrett食管(BE组)和6例食管腺癌(EA组)和20例慢性浅表性胃炎(正常对照组)的食管黏膜组织进行活检,免疫组化法和免疫印迹法测定各组黏膜组织食管上皮细胞PPARγ和增殖细胞核抗原(PCNA)的蛋白表达.结果 免疫组化法显示RE、BE、EA组PPARγ的阳性表达率分别为25%、30%、33%,均高于正常对照组的10%(P<0.05);免疫印迹法显示RE、BE、EA组的PPARγ蛋白吸光度比值分别为0.28±0.15、0.21±0.18、0.32±0.13,均高于正常对照组的0.08±0.06(P<0.05);RE、BE、EA组3组间的PPARγ表达差异无统计学意义(P>0.05).免疫组化法显示正常对照组、RE组、BE组、EA组PCNA的阳性表达率分别为25%、60%、75%、100%.结论 从正常食管到反流性食管炎、Barrett食管、食管腺癌,食管上皮的细胞增殖明显增加,伴随着PPARγ蛋白的表达增加,提示PPARγ可能参与了反流性食管炎、Barrett食管、食管腺癌发病过程中细胞增殖的调控.     

Expression of TRAIL and its receptors in primary hepatic carcinoma and apoptosis-inducing effect of HrsTRAIL on hepatoma cell line HepG2

Objective： To investigate the expression of tumor necrosis factor-related apoptosis-inducing ligand（TRAIL） and its receptors in primary hepatic carcinoma （PHC） and the apoptosis-inducing effect on hepatoma cell line HepG2. Methods： TRAIL and its receptors were detected by semiquantitive RT-PCR in 30 PHC and para-carcinoma tissues and two hepatoma cell lines of HepG2 and SMMC-7721. HepG2 cells were treated with human recombinant soluble TRAIL protein （HrsTRAIL） and then the viability of HepG2 cells was measured by microculture tetrazolium dye（MTT） assay and apoptosis index was demonstrated by fluorescence-activated cell sorting （FACS）. Results：TRAIL and its receptors were detectable in all PHC and para-carcinoma tissues and hepatoma cell line HepG2. TRAIL, death receptor 4 （DR4）, DR5, and decoy receptor 2 （DcR2） but not DcR1 were detectable in hepatoma cell line SMMC-7721. The expression patterns of TRAIL receptors in HepG2 were quite similar to PHC specimens. The semiquantitive results showed that the expression level of TRAIL and DcR were lower but DR was higher in hepatoma tissues than in para-carcinoma tissues. In PHC tissues, the expressions of DR were higher than DcR, while there was no difference in para-carcinoma tissues. HrsTRAIL had potent antitumor activity in a time- and dose-dependent manner. After co-incubations of the HepG2 cells in the presence of HrsTRAIL at concentration 1 000 ng/ml for 24 hours, the viability of HepG2 cells decreased to 45% and the apoptosis index reached 51%. Conclusion ：TRAIL and its receptors were expressed in both PHC tissues and para-carcinoma tissues but the expression levels were different. The lower expression of TRAIL in PHC tissues suggested that insufficient apoptosis occured in the development of PHC. High expression of DR in PHC tissues may be a self-defense mech- anism and may afford a theory of HrsTRAIL therapy for PHC. HrsTRAIL may be a potential cytotoxic drug for PHC, and it can kill majority of HepG2 cells, but minority of HepG2 were resistant to TRAIL-inducing apoptosis.     

Effects of allitridin on the expression of transcription factor T-bet mRNA of cytomegalovirus-induced myocarditis in mice

INTRODUCTIONT he cardiovascular disease myocarditis is character ized by inflammation and necrosis of cardiac mus cle. The disease ranges from transient inflammation to afulminant syndrome with manifestations that may includeheart failure, arrhythmias, and sudden death. This dis ease has been associated with various viral etiologies.Although enterovirus infections are recognized as a lead ing cause of myocarditis, at the present studies havedemonstrated that cytomegalovirus…     

The abnormal expression of E-cadherin in intrahepatic bile duct epithelia cells in biliary atresia and its relationship with apoptosis

The abnormal expression of E-cadherin in intrahepatic bile duct epithelia cells in biliary atresia and its relationship with apoptosis@黄磊$Dept Pediatr Surg,Tongji Hosp Huazhong Univ Sci Technol,Wuhan 430030     

Effects of Intrinsic Nitric Oxide on the Expression of Interleukin-4 and IFN-γ mRNA in the Bronchial and Lung Tissues of Sensitized Rats

Airway infiltration by inflammatory cells,particularly of eosinophils (Eos) and lymphocytes,is one of the features of asthma. Several mechanisms responsible for the attraction and localizationof inflammatory cells at site of allergic reactionhave been proposed. Among them, attention ispresently focused on the hypothesized switching ofCD4 T lymphocyte. Local inflammation of activated T cells (CD25 ) and ThZ--like cytokinesplay a very important role in the development ofasthmatic airway i…     

The effect of reactive oxygen species of inducing apoptosis on the hepatocacinoma in the rabbits

Objective: To study the effect of reactive oxygen species of inducing apoptosis on the heptocacinoma tissues following ischemia and reperfusion and perfusion hyperoxia liquid of hepatocarcinoma. Methods: The hepatocarcinoma animal models ware established by implantation of VX2 tumor constitution mass into the left middle lobe of liver of rabbits. The animals were subjected to 60 min clamp-induced ischemia of hepatic artery distributing in the left middle lobe followed by reperfusion atlh, Id, 3d and 7 d, respectively, and perfusion hyperoxia liquid (partial pressure of oxygen, PO2＞80 kPa) at the same time with reperfusion beginning. The concentration of MDA and NO ware tested. Apoptotic changes in the hepatocarcinoma and normal hepatic tissues were observed by means of HE staining and terminal de-oxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNED. Results: The concentration of MDA in normal hepatic tissues and hepatocarcinoma tissue increased followed ischemia and reperfusion especially for reperfusion 1 h (4. 61 ±0. 40, 3. 10±0. 23) and restored to normal level at reperfusion 7 d in normal hepatic tissues but still kept high concentration in the hepatocarcinoma tissue. Even though concentration of MDA in normal hepatic tissues is higher than that of before ischemia and reperfusion, no difference have been found after perfusion of hyperoxia liquid, and in the hepatocarcinoma tissue. the increasing of concentration of MDA was obvious after simply ischemia and reperfusion at reperfusion Id (4. 25 ± 0. 45). The concentration of NO in normal hepatic tissues increased for reperfusion 3 d and 7 d(18. 17± 0. 13, 17. 45±0. 23),while that of hepatocarcinoma tissue decreased at reperfusion 3 d(15. 95±043). After perfusion of hyperoxia liquid, the concentration of NO in normal hepatic tissues kept increasing and that decreased in the hepatocarcinoma tissues in all time point and reached the lowest level at reperfusion 1 d(14. 62±O. 45). The result demonstrated the changes of concentration of NO and MDA in the hepatocarcinoma tissues ware more obvious than that of normal hepatic tissues(P＜0. 01). Conclusion: Perfusion of hyperoxia liquid from hepatic portal vein can intensify ischemia and reperfusion injury but less so for normal hepatic tissues.     

The expression of P63 protein in some keratinocyte original tissues and cells

Objective： To examine the expression patterns of p63 in tissues of particular keratinocyte original hyperproliferate diseases and variety cell types for determining if P63 is the marker of proliferative potential keratinocytes. Methods： P63 protein was detected and analyzed by immunoreactivity method and Western blot in biopsy specimens of keratinocyte original disorders including squamous cell carcinomas SCC, basal cell carcinomas BCC, Bowen＇ s disease and other tissues or cells, such as psoriasis vulgaris, normal skin tissues, primary cultured keratinocytes, immortal HaCaT cells, and epidermoid carcinoma cells A431. Results： P63 protein was expressed in the nuclei of basal and suprabasal layer of the epidermis, germinative cells of sebaceous glands in normal epidermal. P63 was strongly and diffusely detected in the majority of tumor cells in BCC and poorly-differentiated SCC. In Bowen＇ s disease, p63 expresses are remarkable in all cell layers. In the psoriasis plaque epidermal, p63 expressed mainly in basal cells and part of spinous cells. P63 expressed more strongly in primary cultured keratinocytes than in A431 cells or HaCaT cells. Conclusion： P63 is a nuclei marker of undifferentiated keratinocytes with the proliferative potential and may disrupt the terminal differentiation. The overexpression of p63 reflects immaturity of the tumor cells. The immunohistochemical staining of p63 may be useful for investigating the origin and differentiation of tumor cells.[第一段]     

Construction of p53 antisense RNA expression vector and its effect on colon cancer cells

Ｃｏｎｓｔｒｕｃｔｉｏｎｏｆｐ５３ａｎｔｉｓｅｎｓｅＲＮＡｅｘｐｒｅｓｉｏｎｖｅｃｔｏｒａｎｄｉｔｓｅｆｅｃｔｏｎｃｏｌｏｎｃａｎｃｅｒｃｅｌｓＣａｏＪｉａｎｇ曹江，ＴｅｎｇＬｉｓｏｎｇ滕理送，ＺｈｅｎｇＳｈｕ郑树，ＣａｉＸｉｎｈａｎ蔡心涵ａｎｄＧｅ...     

Effect of Danshensu on expression of STAT1, PPARγ and hepatocyte growth factor in the liver of mice with streptozotocin-induced diabetes

目的 观察丹参素对链脲佐菌素(STZ)诱导的糖尿病小鼠肝脏组织中信号转导和转录活化因子1(STATl)、过氧化物酶体增殖物激活受体γ(PPARγ)以及肝细胞生长因子(HGF)表达的影响,探讨其对糖尿病小鼠肝脏保护作用的机制.方法 60只健康雄性C57BL/6小鼠中随机选取10只作为正常对照组,剩余50只小鼠按200 mg/kg一次性腹腔注射STZ500mg,72 h后尾尖取血测空腹血糖,血糖值大于20 mmol/L为糖尿病小鼠.40只糖尿病小鼠随机分为糖尿病模型组,丹参素低剂量组(15 mg/kg)、中剂量组(30mg/kg)、高剂量组(60mg/kg).丹参素组小鼠1次/d灌胃给药,连续给药12周,末次给药12 h后检测空腹血糖、胰岛素、糖基化血红蛋白(GHb)的水平,及天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)水平.用蛋白质印迹分析法检测小鼠肝脏组织中STAT1、PPARγ和HGF蛋白的表达.结果 (1)与正常对照组相比,糖尿病模型组及丹参素组的血糖及GHb水平均增高(P＜0.01),胰岛素水平降低(P＜0.01),丹参素各剂量组间血糖、胰岛素及GHb水平差异无统计学意义.(2)糖尿病模型组血清AST、ALT水平较正常对照组升高(P＜0.05);丹参素组血清AST、ALT水平较糖尿病模型组下降(P＜0.05);丹参素各剂量组间AST、ALT水平差异无统计学意义.(3)糖尿病模型组小鼠肝组织中STAT1表达高于正常对照组(P＜0.01),而PPARγ和HGF的表达则降低(P＜0.01);与糖尿病模型组比较,丹参素组的小鼠肝脏组织中PPARγ和HGF的表达上调(P＜0.01),STAT1表达减少(P＜0.01),且呈剂量依赖性.结论 丹参素对糖尿病肝损害的保护作用可能与上调糖尿病小鼠肝脏组织中PPARγ和HGF的表达,抑制STAT1介导的炎症反应有关.     

The effect of thyrotropin receptor antibodies on the proliferation of FRTL-5 cells and the expression of protooncogene c-fos mRNA.

ＴｈｅｅｆｅｃｔｏｆｔｈｙｒｏｔｒｏｐｉｎｒｅｃｅｐｔｏｒａｎｔｉｂｏｄｉｅｓｏｎｔｈｅｐｒｏｌｉｆｅｒａｔｉｏｎｏｆＦＲＴＬ５ｃｅｌｓａｎｄｔｈｅｅｘｐｒｅｓｉｏｎｏｆｐｒｏｔｏｏｎｃｏｇｅｎｅｃｆｏｓｍＲＮＡＦａｎｇＰｅｉｈｕａ方佩华，ＬüＭ...     

The effect of intracellular superoxide anion radical on the expression of hcl-2, p53 and c-Ha-ras in Eca-109 esophageal carcinoma cells

Oxygenfreeradicalsarenormalintracellularby--productofcellaerobicmetabolism,whichhavemanybiologicaleffects,andareassociatedwithmanyphysiologicalorpathologicalprocessinorganism['j.Oncogeneareimportantgenesincellproliferation,differentiation,andapoptosis.Theexpressionofoncogenestakespartintheregulationoftheprocessesabove.Inordertodisposethemolecularmechanismofoxygenfreeradicals'biologicaleffects,wehaveobservedtheinfluenceofOiontheexpressionofhcf--2.p53andc--Haarraswhichisassociatedwithapoptosisa…     

The effect of Nifedipine on the expression of type I collagen in gingival fibroblasts

Objective:To investigate the effect of nifedipine(calciumchannel blocker)on the expression of collagen in gingival fibroblasts invitro.Methods:Primarily gingival fibroblasts were cultured and incubated with various concentrations of nifedipine(108μg/L,360μg/L and 1200μg/L)for 5 days.Gingival fibroblasts were primarily cultured derived from nifedipine responders and non-responders in the presence of 360μg/L nifedipine.Enzyme-linked immunosorbent assay was used to evaluate the amount of type I collagen.Cell proliferation was measured by cell counting with evaluating MTT value.Results:The expressions of collagen and cell proliferation were significantly different among the high concentration groups and the others on the fifth day,especially higher in 360μg/L and 1200μg/L groups and also different among nifedipine responders and non-responders.Conclusion:The expression of collagen and cell proliferation may be concerned with the biological mechanism for gingival overgrowth.     

Expression of NF-kB in Schwann apoptosis in spinal cord following cells and its effect on motor neuron sciatic nerves injury in rats

Objective：To explore the expression of nuclear factor-kappa B （NF-kB） in Schwann cells （SCs） and its effect on motor neuron apoptosis in spinal cord following sciatic nerves injury in adult rats. Methods： Thirty-six adult Sprague-Dawley （SD） rats were divided randomly into normal control group （n=6）, and sciatic nerves crushing group （n= 30）, and the later was further equally randomized into 5 subgroups： 1, 3, 7, 14, and 21 d post-injury groups. The expression of NF-kB of normal and injured nerves were examined by immunohistochemistry staining, and the apoptosis of motor neurons in spinal cord of lumbar 4 to lumbar 6 （L4-L6） was investigated by terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling （TUNEL） assay. Both were qua.ntitated by image analysis. Results： In crushing group, except 21 d post-injury group, the expression of NF-kB was markedly higher than that in the normal control group （P〈0.05, P〈0. 01）. At 1 d after sciatic nerves crushing, the expression of NF-kB was obviously up-regulated, reached peak at 3 d, and recovered at 21 d. The same trend was observed in the time-course on motor neuron apoptosis after sciatic nerves injury. Correlation analyses revealed that motor neuron apoptosis was significantly and positively correlated with the expression of NF-kB following sciatic nerves injury （r= 0. 976 0, P〈0. 01）. Conclusion： After injury of sciatic nerves, the presence and up-regulation of NF-kB in SCs may be involved in motor neuron apoptosis in L4-L6 spinal cord.     

Effects of intrinsic nitric oxide on the expression of interleukin-4 and IFN-γ mRNA in the bronchial and lung tissues of sensitized rats

Summary To investigate the effects of intrinsic nitric oxide (NO) on the expression of interleukin-4 (IL-4) mRNA and interferon-γ (IFN-γ) mRNA in the airway inflammation of asthma, the rat models of asthmatic inflammaiton were established by sensitizing and then challenging the animals with ovalbumin. The 24 animals were randomly divided into control group, sensitized group, sensitized and L-Arg-treated group as well as L-NAME-treated group equally. By using in situ hybridization combined with compute physiological quantitative imaging analysis techniques, the influence of intrinsic NO on the expression of IL-4 mRNA and IFN-γ mRNA in the airway inflammatory cells was observed. In situ hybridization study demonstrated that IL-4 mRNA expression was obviously increased as compared with that in the control group, mainly distributed in the inflammatory cells in the submucous of airways in the sensitized group. The increase of intensity of IL-4 mRNA expression was positively correlated with the numbers of eosinophil (Eos) and lymphocyte (both with P < 0.05) in the sensitized group. There was no statistically difference in IFN-γ expression between the control group and the sensitized group. Imaging analysis showed that L-NAME could inhibit the expression of IL-4 mRNA (P < 0.05) and increase the expression of IFN-γ mRNA (P < 0.05), while L-Arg could increase the expression of IL-4 mRNA in inflammatory cells (P < 0.05). It was indicated that a suitable levels of intrinsic NO can influence the expression of IL-4 mRNA of Th2 lymphocytes and the expression of IFN-γ mRNA of Thl lymphocytes and in turn, promote the development of asthmatic airway inflammation.     

The effect of anastrozole on mRNA expression of oestrogen related gene in MCF-7 breast cancer cells

Objective： To look for additional markers of molecular biology response to anastrozole, a new aromatase inhibitor, on the growth and mRNA expression level of MCF-7 cell. Methods： We investigated the effect of anastrzole on growth and gene expression in the human breast cancer cell line MCF-7 and compared with the most widely used antiestrogen tamoxifen. We chose 4 genes to examine regulation of gene expression of estrogen regulated genes ： PR A, PR B, ErbB-2 and cyclin D1. Results： Compared with the tamoxifen, a statistically significant growth inhibition was observed with anastrozole. The PR A, PR B and cyclin D1 mRNA level in anastrozole treated cells was sigificantly below the level in tamoxifen treated cells （P〈0. 05）. They had agonistic effect on ErbB gene （P〉0. 05）. Conclusion： The third generation of aromatase inhibitors anastrozole exert more inhibit function in some expression of estrogen regulated genes than tomoxifen in MCF-7 cell line.