Effects of Xinfeng capsule on pulmonary function based on treg-mediated notch pathway in a rat model of adjuvant arthritis

Objective

To observe the impact of xinfeng xapsule (XFC) on pulmonary function in a rat model of adjuvant arthritis (AA) and to investigate the mechanism of action.

Methods

Forty rats were randomly divided into four groups of ten: normal control (NC); model control (MC); tripterygium glycosides tablet (TPT); and xinfeng capsule (XFC). Except for the NC group, AA was induced in all rats by intracutaneous injection of 0.1 mL Freund's complete adjuvant in the right paw on the 19th day. NC and MC groups were given (0.9%) physiological saline. The TPT and XFC groups were given TPT (10 mg/kg) and XFC (1.2 g/kg), respectively. Thirty days after administration, changes in paw edema (E), the arthritis index (AI), pulmonary function, levels of regulatory T-cells (Treg), ultrastructure of lung tissue, and expression of Notch receptors and ligands in lung tissue were observed.

Results

In the MC group, E and the AI were increased and pulmonary function significantly decreased; the structure of alveolar type-II cells was damaged; ratios of Treg in peripheral blood were reduced; and expression of Notch receptors such as Notch3 and Notch4 and ligands such as Delta1 in lung tissue were significantly increased whereas expression of Notch1, Jagged1 and Jagged2 were significantly decreased. After intervention with XFC, E and the AI were decreased; pulmonary function was enhanced; the structure of alveolar type-II cells was improved; and expression of Treg, Notch1, Jagged1, Jagged2 was elevated, whereas that of Notch3, Notch4 and Delta1 was reduced.

Conclusion

XFC can not only inhibit E and the AI and improve joint symptoms, it can also improve pulmonary function and reduce inflammation in lung tissue. These actions could be carried out through increases in the expression of Treg, Notch receptors (Notch1) and ligands (Jagged1, Jagged2), and reductions in the expression of Notch3, Notch4 and Delta1. These phenomena would reduce the deposition of immune complexes and the inflammatory response in lung tissue, thereby improving joint symptoms and pulmonary function.     

Use of Xinfeng capsule to treat abarticular pathologic changes in patients with rheumatoid arthritis

OBJECTIVE： To observe the influence of Xinfengcapsule（XFC） on abarticular pathologic changes（APCs） and other indices of patients with rheumatoid arthritis（RA） and explore the mechanism of action of XFC in improving such changes.METHODS： Three-hundred RA patients were divided randomly into a treatment group（n=150） and control group（n=150）. A normal control（NC）group（n=90） was also created. Changes in cardiac function, pulmonary function, anemia indices and platelet parameters of RA patients were measured.Curative effects of the two groups were compared,and comparison carried out with the NC group.RESULTS： In 300 RA patients, late diastolic peak flow velocity（A peak） was much higher（P〈0.01）and early diastolic peak flow velocity（E peak）, E/A,and left ventricular fraction shortening much lower（P〈0.01） than those in the NC group. Vital capacity（VC）, forced vital capacity in one second, forced vital capacity（FVC）, maximal voluntary ventilation（MVV）, maximal expiratory flow in 50% of VC（FEF50） and FEF75 were lowered remarkably（P〈0.05 or P〈0.01）. Platelet count（PLT）, plateletcrit（PCT） and mean platelet volume（MPV） increased markedly（P〈0.05 or P〈0.01）, and hemoglobin（Hb）level decreased significantly（P〈0.05）. After XFC treatment, the A peak and PLT and PCT were much lower（P〈0.05）, and E/A and the number of red blood cells as well as Hb level were much higher（P〈0.05）, as were FVC, MVV and FEF50（P〈0.05 or P〈0.01）, in the treatment group than those in the NC group. Total score of pain and swelling in joints,uric-acid level and high-sensitivity C-reactive protein level were much lower, and superoxide dismutase level as well as the number of CD4 ＋CD25＋ regulation T cells（Treg） and CD4＋CD25＋CD127- Treg were much higher（P〈0.05 or P〈0.01）in the treatment group than those in the NC group.CONCLUSION： RA patients with pathologic changes in joints also suffer from lower cardiac and pulmon     

新风胶囊对活动期类风湿关节炎抑郁患者的影响

[目的]观察中药新风胶囊(XFC)对活动期类风湿关节炎(RA)伴抑郁情绪患者的影响,并探讨其作用机制.[方法]40例RA患者按随机数字表分成2组,治疗组20例采用XFC,对照组20例采用风湿骨痛胶囊,观察2组的主要症状体征、生活质量、抑郁自评量表(SDS)标准分及实验室指标等的变化.[结果]XFC在改善症状体征、活动性指标等方面与风湿骨痛胶囊相似.但在改善SDS标准分、脾虚症状、生活质量积分、血清铁方面优于风湿骨痛胶囊对照组.[结论]XFC可改善RA患者抑郁情绪,提高生活质量.提高机体免疫功能、改善生活质量和贫血症状等整体调节是其可能作用机制.     

咳喘宁胶囊对慢性支气管炎患者肺功能和免疫功能影响的研究

目的：观察咳喘宁胶囊对慢性支气管炎患者肺功能和免疫功能的影响。方法：治疗组64例,口服该喘宁胶囊,对照组44例,用桂龙咳喘宁胶囊治疗。治疗前及疗程结后检测肺功能及免疫各项指标。结果：2组药物均能有效改善慢性支气管炎患者 的用力肺活量（FVC）、用力肺活量占预计值百分比（FVC％）、第1秒时间肺活量（FEV1）、第1秒时间肺活量占预计值百分比（FEV1％）、第1秒时间肺活量占用力肺活量比值与相应预计值百分比（FEV1／FVC％）（P＜0．05）或P＜0．01）,并能显著提高血清免疫球蛋白IgG、IgM（P＜0．05）。结论：咳喘宁胶囊可通过改善机体肺功能和体液免疫功能状态,对慢性支气管炎产生良好的治疗作用。     

肺力咳胶囊联合沙丁胺醇治疗慢性阻塞性肺疾病疗效及对患者肺功能的影响

目的:探讨肺力咳胶囊联合沙丁胺醇治疗慢性阻塞性肺疾病(COPD)疗效及对患者肺功能影响。方法:选取80例COPD患者为研究对象,根据不同治疗方法分为研究组和对照组,每组患者40例。对照组患者采取沙丁胺醇治疗,研究组患者采取肺力咳胶囊联合沙丁胺醇治疗。比较两组患者临床疗效和肺功能。结果:研究组患者的治疗总有效率高于对照组(P<0.05)。两组患者治疗后TNF-α、hs-CRP、IL-4、FEV₁、FVC、PEF均改善,且研究组患者各炎性指标均低于对照组,各肺功能指标均高于对照组,两组比较差异均具有统计学意义(P<0.05)。研究组和对照组不良反应发生率分别为7.5%和10%,两组比较差异无统计学意义(P>0.05)。结论:肺力咳胶囊联合沙丁胺醇治疗COPD疗效较好,可改善患者临床症状,减少气道炎性反应,改善肺功能。     

Effect of cold-dryness on pulmonary and immunologic function in chronic obstructive pulmonary disease model rats

OBJECTIVE： To study the effects of cold-dryness on pulmonary and immunologic function of peripheral T-lymphocytes in chronic obstructive pulmonary disease （COPD） model rats, and to provide references for the prevention and treatment of cold-dryness COPD in the Xinjiang region. METHODS： The COPD model was established with an elastase drip into the trachea combined with smoking. The cold-dryness COPD model was developed by stressing with a cold-dry environment. Success of the model was determined by observation of pathologic lung sections. Rats were sacrificed by exsanguination from the femoral artery and changes of peripheral blood CD4＋, CD8＋, and CD4＋/CD8＋ were detected by flow cytometryo Data were analyzed with SAS 11.5 statistical software. RESULTS： On the ninetieth day after ending the ex- periment, Peak expiratory flow in the cold-dryness COPD group was lower than that in the COPD and normal control groups （P〈0.01）. The time of inspiration in the cold-dryness COPD group was higher than that in the COPD and normal groups （P〈0.05）. Time of expiration （Te） in the cold-dryness COPD group was higher than that in the COPD and normal groups （P〈0.01）. 50% tidal volume expiratory flow （EFS0） in the cold-dryness COPD group was lower than that in the COPD and normal groups （P〈 0.01）, and EFS0 in the COPD group was lower than that in the normal group （P〈0.05）. CD4＋ content of peripheral blood in the cold-dryness COPD group was lower than that in the COPD and the normal groups （P〈0.05）. CD8＋ content in the cold-dryness COPD and COPD groups was higher than that in the normal control group （P〈0.01）, and CD8＋ content in the cold-dryness COPD group was higher than that in the COPD group （P〈0.01）. CD4＋/CD8＋ in the cold-dryness COPD group and the COPD group was lower than that in the normal control group （P〈0.01）, and CD4＋/CD8＋ in the cold-dryness COPD group was lower than that in the COPD group （P〈0.05）. CONCLUSION： In the cold-dryness COPD model, CD8＋ increased and CD4＋/CD8＋ decreased. Moreover, cold-dryness may aggravate this state. The effects of cold-dryness on pulmonary function main- ly manifested as prolongation of Te and decrease of EF50, which could be one of causes of cold-dryness environment in the northwest of China leading to COPD with region characteristics.     

Effect of cold-dryness on pulmonary and immunologic function in chronic obstructive pulmonary disease model rats

Objective

To study the effects of cold-dryness on pulmonary and immunologic function of peripheral T-lymphocytes in chronic obstructive pulmonary disease (COPD) model rats, and to provide references for the prevention and treatment of cold-dryness COPD in the Xinjiang region.

Methods

The COPD model was established with an elastase drip into the trachea combined with smoking. The cold-dryness COPD model was developed by stressing with a cold-dry environment. Success of the model was determined by observation of pathologic lung sections. Rats were sacrificed by exsanguination from the femoral artery and changes of peripheral blood CD4+, CD8+, and CD4+/CD8+ were detected by flow cytometry. Data were analyzed with SAS 11.5 statistical software.

Results

On the ninetieth day after ending the experiment, Peak expiratory flow in the cold-dryness COPD group was lower than that in the COPD and normal control groups (P<0.01). The time of inspiration in the cold-dryness COPD group was higher than that in the COPD and normal groups (P<0.05). Time of expiration (Te) in the cold-dryness COPD group was higher than that in the COPD and normal groups (P<0.01). 50% tidal volume expiratory flow (EF50) in the cold-dryness COPD group was lower than that in the COPD and normal groups (P<0.01), and EF50 in the COPD group was lower than that in the normal group (P<0.05). CD4+ content of peripheral blood in the cold-dryness COPD group was lower than that in the COPD and the normal groups (P<0.05). CD8+ content in the cold-dryness COPD and COPD groups was higher than that in the normal control group (P<0.01), and CD8+ content in the cold-dryness COPD group was higher than that in the COPD group (P<0.01). CD4+/CD8+ in the cold-dryness COPD group and the COPD group was lower than that in the normal control group (P<0.01), and CD4+/CD8+ in the cold-dryness COPD group was lower than that in the COPD group (P<0.05).

Conclusion

In the cold-dryness COPD model, CD8+ increased and CD4+/CD8+ decreased. Moreover, cold-dryness may aggravate this state. The effects of cold-dryness on pulmonary function mainly manifested as prolongation of Te and decrease of EF50, which could be one of causes of cold-dryness environment in the northwest of China leading to COPD with region characteristics.     

新风胶囊对佐剂性关节炎大鼠滑膜细胞及脾脏淋巴细胞超微结构作用的实验研究

目的：观察新风胶囊对佐剂性关节炎大鼠治疗作用及对滑膜细胞、脾脏淋巴细胞超微结构的作用。方法：将60只大鼠随机分为正常对照组、模型对照组、新风胶囊组和雷公藤组，每组15只，制备完全佐剂并分别向除正常对照组以外其余动物右后足跖皮内注射0．1ml致炎。电镜观察滑膜细胞、脾脏淋巴细胞超微结构变化（包括线粒体肿胀、空泡变、嵴突病变等），计算各组线粒体病变率。结果：与治疗前比较，新风胶囊组及雷公藤组治疗后右后足跖肿胀度显著降低（P＜0．05）；与模型对照组比较，新风胶囊组和雷公藤组治疗后模型大鼠关节滑膜细胞线粒体的病变率显著降低（P＜0．05）；新风胶囊组脾脏淋巴细胞线粒体病变率显著降低（P＜0．05），而雷公藤组脾脏淋巴细胞线粒病变率差异无显著性（P＞0．05）。与雷公藤组比较，新风胶囊组治疗后滑膜细胞及脾脏淋巴细胞线粒体病变率显著降低（P＜0．05）。结论：新风胶囊与雷公藤均能降低佐剂性关节炎大鼠病变关节的肿胀度，新风胶囊改善佐剂性关节炎大鼠滑膜和脾脏的超微结构病变的作用优于雷公藤，这可能是新风胶囊治疗作用的形态学基础。     

藏药然降多吉胶囊对大鼠佐剂性关节炎细胞因子及炎症介质的影响

目的 :观察藏药然降多吉胶囊对大鼠佐剂性多发性关节炎的影响以及该药的免疫调节活性。方法 :用弗氏完全佐剂造成大鼠多发性关节炎 ,观察足跖肿胀程度、全身病变、脾脏T淋巴细胞增殖功能、腹腔巨噬细胞白介素 1(IL-1)分泌活性、血清脂质过氧化和超氧化物歧化酶 (SOD-1)活性。结果 :藏药然降多吉胶囊灌胃给药对弗氏完全佐剂所致大鼠继发的关节肿胀有显著的抑制作用 ,降低多发性佐剂性关节炎大鼠脾T淋巴细胞增殖转化率 ,抑制血清脂质过氧化产物的形成 ,对腹腔巨噬细胞产生白细胞介素 1(IL-1)分泌活性亦有一定抑制作用。结论 :然降多吉胶囊对佐剂性关节炎有显著的治疗作用 ,其机理与抑制T细胞增殖以及白细胞介素 1、过氧化脂质产生有关。     

湿迪胶囊对大鼠佐剂性关节炎作用机制的研究

目的：观察湿迪胶囊对大鼠佐剂性关节炎(AA)的作用并初步探讨其作用机制。方法：SD大鼠,雄性,随机分为正常组(0.5% CMC-Na溶液)、模型组(0.5% CMC-Na溶液)、地塞米松组(0.45 mg·kg_-1)、白芍总苷组(150 mg·kg_-1)以及湿迪胶囊(以生药计)高(5 400 mg·kg_-1)、中(2 700 mg·kg_-1)、低(1 350 mg·kg_-1)剂量组；除正常组外,其余各组采用大鼠右后足跖皮内注射氟氏完全佐剂建立AA模型,并分别给予灌胃治疗,观察各组的足跖肿胀度,测定炎性肿胀足前列腺素E₂(PGE₂)含量及血清中丙二醛(MDA)、超氧化物歧化酶(SOD)含量,测定脾及胸腺指数,并观察踝关节的病理组织学变化。结果：湿迪胶囊高、中、低剂量组可剂量依赖性显著抑制大鼠右后足跖的原发性肿胀和左后足跖的继发性肿胀；与模型组比较,湿迪胶囊高、中、低剂量组SOD总活力明显升高,MDA含量明显下降,PGE₂含量降低；与模型组比较湿迪3个剂量组的胸腺和脾脏指数均有所提高,差异有显著意义；湿迪高、中、低剂量组均可明显减轻AA大鼠的病理变化。结论：湿迪胶囊能明显抑制滑膜组织的炎症,减轻细胞的损伤程度,因而对AA大鼠具有一定的治疗效果。     

利生康胶囊对免疫功能的影响

目的 研究利生康胶囊在发挥抗癌作用的同时对机体免疫功能的影响。方法 采用免疫器官质量法、碳粒廓清法、血清溶血素生成法和T_4,T_8,NK活性测定法,对给予利生康胶囊的肿瘤模型小鼠和对照组小鼠的免疫功能进行测定,并对结果进行分析。结果 利生康胶囊能提高小鼠脾指数、胸腺指数和碳粒廓清速率,能提高其血清溶血素(HC_(50))水平和活性,明显降低T_8活性。结论 利生康胶囊在发挥抗癌作用的同时,能提高机体的免疫功能。     

Effect of Jiaweiwumei decoction on regulatory T cells and interleukin-10 in a rat model of ulcerative colitis

OBJECTIVE:To investigate the effect of a decoction made from the Traditional Chinese Medicine Wumei pill,on regulatory T cells and interleukin-10(IL-10) in a rat model of ulcerative colitis induced by2,4,6-trinitrobenzene sulfonic acid(TNBS).METHODS:Rat ulcerative colitis was induced with TNBS.All modeled rats were randomly divided into six groups:normal control group;model group;sulfasalazine suppositories treatment group;and high,moderate,and low dosage of Jiaweiwumei decoction groups(12 rats each).Colon injury index was evaluated after 14 days.After peripheral blood lymphocyte separation,CD4 + T cells and CD4+/CD25+ T cell percentage was detected by flow cytometry.The content of IL-10 in serum and intestinal mucosa tissue was detected by sandwich enzyme-linked immunosorbent assay.RESULTS:Colon injury indices in the decoction groups were effectively reduced,compared with the model group(P 0.05).Compared with that of the control group,the CD4+/CD25+ to CD4+ T lymphocyte ratio of the model group was significantly lower,while the decoction treatment improved the CD4 +/CD25 + to CD4 + T lymphocyte ratio(P 0.05).The serum and mucosal IL-10 content of the model group was significantly lower(P 0.05) than that in the control group,while the decoction group had significantly higher serum and intestinal mucosal IL-10 content than that in the model group(P 0.05).The regulatory T cell content was negatively correlated with the colonic injury index(r = 0.68,P 0.05),and positively correlated with the content of serum IL-10(r = 0.87,P 0.05) and intestinal mucosal IL-10(r= 0.79,P 0.05).CONCLUSION:Jiaweiwumei decoction had significant effects on regulatory T cells and IL-10 in rats with TNBS-induced ulcerative colitis.     

Mechanism studies of Xinfeng capsule on improving cardiovascular function though toll-like receptor 4/nuclear factor kappa B pathway in a rat model of adjuvant arthritis

OBJECTIVE: To observe the impact of Xinfeng capsule(XFC) on cardiovascular function in adjuvant arthritis(AA) model rats and investigate the mechanism though toll-like receptor 4(TLR4)/nuclear factor kappa B(NF-κB) signaling pathway.METHODS: Seventy rats were randomly divided into seven groups: normal control(NC), model control(MC), tripterygium glycosides tablet(TPT),methotrexate(MTX), high, moderate and low dose XFC group. The administration began from day 19 after modeling for 30 day. Paw swelling, arthritic in-dex(AI), cardiac function indexes and myocardial pathological pattern were detected. The expression of TLR4, myeloid differentiation factor(My D) 88, interleukin-1 receptor-associated kinase(IRAK) 1, tumor necrosis factor receptor associated factor(TRAF) 6, NF-κB, tumor necrosis factor-alpha(TNF-α) proteins in myocardial tissue were determined by western blot method.RESULTS: Paw swelling and AI in MC group increased in MC group(P 0.01), and decreased in high and moderate dose XFC groups(P 0.01 or P 0.05). Left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),heart rate(HR) were elevated in MC group(P 0.01), and ± dp/dtmax and CI were reduced(P 0.01); while LVSP, LVEDP and HR declined and ±dp/dtmax, CI improved in high dose XFC group(P 0.05 or P 0.01). LVSP in high dose XFC group were reduced more than other treatment groups(P 0.05 or P 0.01). The improvements on LVEDP, dp/dt-max were superior to MTX and low dose XFC group, and the improvement on CI was better than low dose XFC group(P 0.05 or P 0.01). Myocardial fibers arranged irregular in MC group with intracellular edema and mitochondria damage. The modifications on myocardial structural were shown in each treatment group, but more prominent in TPT, high and moderate dose XFC group. The proteins of TLR4, My D88, IRAK1, TRAF6, NF-κB, TNF-αwere highly expressed in MC group, and those proteins declined in high and moderate dose XFC group(P 0.05 or P 0.01). High dose XFC group was superior to MTX and low dose XFC group on reducing TLR4, NF-κB, TNF-α(P 0.05).CONCLUSION: XFC can not only inhibit the excessive activation of TLR4/NF-κB signaling pathway and the increased inflammatory mediators, but also reduce the damage of myocardial tissue and cells.     

益肺康心胶囊对大鼠慢性肺源性心脏病模型MMP-1的影响

目的 探讨益肺康心胶囊对大鼠肺心病模型MMP-1 的影响.方法 将40 只Wistar 大鼠按体重随机分为空白组、肺心病模型组、治疗组、对照组4 组,每组各10 只.治疗组给予益肺康心胶囊2.6mg/kg,1 次/d;对照组给予硝苯地平2.7 mg/kg 灌胃,1 次/d.连续给药15 d,至检查前24 h 停药.分别于停药后的7 d、14 d、28 d 处死大鼠,测定肺组织MMP-1.结果 治疗组肺组织MMP-1 表达明显下降,第7 天、14 天、28 天分别为(0.231±0.017)ng/ml、(0.308±0.081)ng/ml 、(0.358±0.074)ng/ml,低于肺心病模型组[第7 天、14 天、28 天分别为(0.266±0.036)ng/ml 、(0.315±0.060)ng/ml 、(0.612± 0.091)ng/ml].结论 益肺康心胶囊可明显抑制大鼠肺心病模型MMP-1 的表达.

Abstract：

Objective To discuss the influence of Yifei-Kangxin capsule on MMP-1 in Wistar rats model with pulmonary heart disease. Methods 40 Wistar rats with pulmonary heart disease were averagely grouped as Normal Group, Model Group, Therapeutic Group and Comparative Group randomly by weight. The rats in Therapeutic Group were given Yifei-Kangxin capsule and the rats in Comparative Group were given Nifedipine. The course of treatment was 15 days. The rats were killed at the next 7th, 14th and 28th day separately to test MMP-1 in pulmonary tissue. Results After the treatment, the expression of MMP-1 in pulmonary tissue of the Therapeutic Group[7 d, 14 d, 28 d was(0.231±0.017)ng/m、(0.308±0.081)ng/m、(0.358±0.074)ng/m]was lower than the Model Group[7 d, 14 d, 28 d was(0.266±0.036)ng/m 、(0.315± 0.060)ng/m、(0.612±0.091)ng/m]. Conclusion Yifei-Kangxin capsule can inhibit the expression of MMP-1 in the rats model pulmonary heart disease.     

基于Th1/Th2免疫平衡探讨血栓通胶囊对视网膜静脉阻塞的疗效及机制

目的:探究血栓通胶囊对视网膜静脉阻塞(RVO)患者的治疗效果与作用机制。方法:回顾性选取84例RVO患者作为研究对象,依据治疗方案的不同分为对照组(n=42)与观察组(n=42),对照组采用常规抗血管内皮生长因子(VEGF)治疗,观察组则在对照组基础上给予血栓通胶囊治疗。对比两组患者治疗前后的黄斑中央视网膜厚度(CRT)、视力水平、眼部血流灌注面积、中医症状积分、免疫功能、综合疗效以及用药安全性。结果:治疗后1、3个月,观察组CRT小于对照组(P<0.05); 治疗后1、3个月,观察组最佳矫正视力明显高于对照组(P<0.05); 治疗后3个月,观察组视网膜浅层毛细血管区血流密度(SCP)、深层毛细血管区血流密度(DCP)面积明显增大(P<0.05),中心凹无血管区面积(FAZ)明显缩小(P<0.05),与对照组比较差异有统计学意义(P<0.05)。治疗后1、3个月,观察组中医症状积分明显低于对照组(P<0.05); 观察组治疗,总有效率明显高于对照组(P<0.05)。治疗后,观察组IgA、IgM、IgG、Th₁/Th₂水平均高于对照组(P<0.05)。两组患者在治疗过程中均无不良反应及不良事件发生。结论:血栓通胶囊治疗RVO疗效显著,其机制可能与调节Th₁/Th₂平衡有关。     

新风胶囊对佐剂关节炎大鼠肺功能及Notch信号传导通路的影响

目的：观察新风胶囊对佐剂关节炎（AA）大鼠足跖肿胀度（E）、关节炎指数（AI）、肺系数（LI）、肺功能变化、调节性T细胞（Treg）及肺组织Notch受体、配体表达的影响。方法：将84只大鼠随机分为正常对照（NC）组、模型（Model）组、甲氨喋呤(MTX)组、雷公藤多苷片（TGT）组和新风胶囊低剂量（XFC-L）组、新风胶囊中剂量（XFC-M）组、新风胶囊高剂量（XFC-H）组,每组12只,分别给动物右后足跖皮内注射弗氏完全佐剂（FCA）0.1mL致炎（除NC组外）,致炎后第19d开始给药。NC组及Model组均给予生理盐水,其余5组分别给予MTX、TGT、XFC。观察各组大鼠上述各指标的变化。结果：①与NC组相比,Model组大鼠E、AI、LI在1s内平均呼气流量（FEV1/FVC）、肺泡炎积分、Notch3、Notch4、Delta1表达明显升高（P<0.01）;用力肺活量（FVC）、25%肺活量的最大呼气流量（FEF25）、50%肺活量的最大呼气流量（FEF50）、75%肺活量的最大呼气流量（FEF75）、最大呼气中期流量（MMF）、用力最大呼气流量（PEF）、CD4+ T细胞、CD25+ T细胞、CD4+ CD25+ Treg、Notch1、Jagged1、Jagged2表达明显降低（P<0.01）。②与Model组相比,各治疗组大鼠体重、FVC、FEF25、FEF50、FEF75、MMF、PEF、Notch1、Jagged1、Jagged2及Treg的表达升高;E、AI、LI、FEV/FVC及Notch3、Notch4、Delta1的表达降低（P<0.05或P<0.01）。③与XFC-M组相比,其余各治疗组LI、肺泡炎积分及Notch3、Delta1升高;FVC、FEF25、FEF50、FEF75、MMF、PEF及Notch1、Jagged1、CD4+ T细胞、CD4+ CD25+ Treg降低（P<0.05或P<0.01）。结论：AA大鼠存在关节局部的炎症反应和肺功能的降低,通过相关分析得知肺功能降低与Notch信号传导通路的表达有关;新风胶囊能明显改善AA大鼠关节症状和肺功能,可能通过促进Delta1、Notch3、Notch4的表达、抑制Jagged1、Jagged2、Notch1表达,降低炎症反应和免疫复合物沉积,改善关节和肺部症状。     

黄连素合用环孢素A对小鼠皮肤移植排斥反应的影响

目的观察黄连素合用环孢素A抗同种异体小鼠皮肤移植排斥反应的影响,探讨其作用机制。方法以BALB/c小鼠为供体,以C57BL/6小鼠为受体,采用背-背皮肤移植手术法制备模型。将受体小鼠按随机数字表法分为模型组、黄连素组、环孢素A组、联合组,每组20只;另取20只健康C57BL/6小鼠作为假手术组。造模后黄连素组小鼠腹腔注射黄连素100 mg/kg,环孢素A组小鼠腹腔注射环孢素A注射液10 mg/kg,联合组腹腔注射黄连素100 mg/kg及环孢素A注射液5 mg/kg,假手术组和模型组小鼠腹腔注射等体积0.5%羧甲基纤维素钠。1次/d,连续10 d。记录受体小鼠移植皮片的存活时间;采用ELISA法检测血浆Th1类细胞因子[IL-2、γ-干扰素(interferon-γ,IFN-γ)]和Th2类细胞因子(IL-4、IL-10)水平;采用流式细胞术检测脾脏T淋巴细胞亚群CD4+CD25+比例。结果与模型组比较,各给药组受体小鼠移植皮片的存活时间延长(P<0.01);联合组移植皮片存活时间较黄连素组或环孢素A组延长(P<0.01)。与模型组比较,联合组血浆IL-2[(11.55±3.14)pg/ml比(19.85±2.42)pg/ml]、IFN-γ[(26.41±6.20)pg/ml比(57.23±10.15)pg/ml]水平降低,IL-4[(192.45±70.12)pg/ml比(61.09±21.61)pg/ml]、IL-10[(106.79±27.83)pg/ml比(40.08±11.23)pg/ml]水平升高(P<0.05),脾脏CD4+CD25+调节性T细胞比例[(7.65±2.42)%比(3.69±0.83)%]升高(P<0.01)。结论黄连素合用环孢素A通过促使Thl向Th2细胞的偏移、诱导免疫耐受、刺激CD4+CD25+调节性T细胞的表达等途径,抑制同种异体小鼠的皮肤移植排斥反应。     

蒙药五味风湿胶囊对佐剂性关节炎的作用及机制研究

目的:观察蒙药五味风湿胶囊对佐剂性关节炎的作用及其免疫调节机制.方法:取Wistar大鼠,分成正常组、佐剂性关节炎(AA)模型组、五味风湿胶囊低剂量组(0.2g.kg-1.d-1)、中剂量组(0.4g· kg-1.d-1)、高剂量组(0.8 g.kg-1.d-1)及忠伦-5汤组(原剂型1.68 g·kg-1·d-1),测定各组大鼠关节肿胀程度,关节组织白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、前列腺素(PGE2)及一氧化氮(NO)水平,测定血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)水平.通过细胞培养,考察药物对AA大鼠脾细胞增殖能力的影响,探讨该药干预下从大鼠腹腔巨噬细胞培养液对C57BL/6J小鼠胸腺细胞增殖能力的影响.结果:五味风湿胶囊可抑制AA大鼠关节组织IL-1β,TNF-α,PGE2,NO水平;提高血清SOD活性,但MDA未见显著变化;可抑制AA大鼠脾细胞增殖能力;抑制AA大鼠腹腔巨噬细胞培养液对C57BL/6J小鼠胸腺细胞增殖能力的影响.结论:五味风湿胶囊具有良好的抗佐剂性关节炎作用.其作用机制可能与抑制相关细胞因子,调节相关酶的活性及免疫器官细胞增殖等有关.     

肺泰胶囊联合母牛分枝杆菌对老年肺结核患者免疫功能的影响

目的：探讨肺泰胶囊联合母牛分枝杆菌对老年肺结核患者免疫功能的影响。方法选择2012年1月－2013年10月河北省胸科医院胸三科住院患者100例,按随机数字表法随机分为2组各50例。对照组采用常规抗结核方案（2HRZE/4HR）治疗;治疗组在对照组基础上加服肺泰胶囊和肌肉注射母牛分枝杆菌治疗。2组均治疗6个月后行痰涂片检测,外周血CD8＋、CD3＋、CD4＋ T细胞水平检测以及CD4＋/CD8＋比值测定,并评价临床疗效。结果治疗组治疗后CD3＋[(65.4±6.6)%比(58.1±6.2)%;t＝5.803, P＝0.000]、CD4＋[(38.6±5.7)%比(26.6±4.6)%;t＝11.836,P＝0.000] T 细胞水平以及CD4＋/CD8＋比值[(1.6±0.3)比(0.9±0.2);t＝14.329,P＝0.000]均较治疗前升高,而CD8＋ T细胞水平则低于治疗前[(25.6±4.2)%比(32.5±4.2)%;t＝8.355,P＝0.000];对照组CD3＋[(60.7±5.4)%比(58.3±6.2)%;t＝2.072, P＝0.000]、CD8＋[(30.2±4.2)%比(32.3±4.9)%;t＝2.331,P＝0.000] T细胞水平以及CD4＋/CD8＋比值[(1.1±0.2)比(0.9±0.2);t＝4.488,P＝0.000]与治疗前比较,差异均有统计学意义。治疗组治疗后CD3＋、CD4＋、CD8＋ T细胞水平及CD4＋/CD8＋比值与对照组治疗后比较,差异均有统计学意义（t值分别为3.920、11.966、5.573、10.700,P＜0.01）。治疗组治愈率为80.0%（40/50）,对照组为56.0%（28/50）,2组比较差异有统计学意义（χ2＝5.561,P＝0.018）。治疗后6个月,治疗组氮阴转率（72.0%比50.0%;χ2＝5.086,P＝0.024）和空洞愈合率（36.0%比16.0%;χ2＝5.198,P＝0.023）均显著高于对照组。结论肺泰胶囊联合母牛分枝杆菌可明显提高老年肺结核患者的免疫功能。