翼核果中化学成分的研究Ⅰ

本文从广西产翼核果（ＶｅｎｔｉｌａｇｏｌｅｉｏｃａｒｐａＢｅｎｔｈ．）根茎的抗炎有效部位碱提取酸沉淀中分离得到了６个化合物，其中４个蒽醌类化合物：大黄素（１），大黄素－８－Ｏ－β－Ｄ－葡萄糖甙（ｅｍｏｄｉｎ－８－Ｏ－β－Ｄ－ｇｌｕｃｏｓｉｄｅ）（２），大黄素－６，８－二甲醚（ｅｍｏｄｉｎ－６，８－ｄｉｍｅｔｈｙｌｅｔｈｅｒ）（３），１－羟基蒽醌（１－ｈｙｄｒｏｘｙａｎｔｈｒａｑｕｉｎｏｎｅ）（４），１个酮类化合物：１，６－二羟基－３－甲基酮－８－羧酸（ｃａｌｙｘａｎｔｈｏｎｅ）（５），及１个异黄酮类化合物：鸢尾甙元（ｔｅｃｔｏｒｉｇｅｎｉｎ）（６）．其中化合物（２），（５）为本种植物中首次分离得到，（６）为本属植物中首次分离得到。     

蛇毒中鞣花酸类及三萜类成分研究

自蛇毒（ＤｕｃｈｅｓｎｅａｉｎｄｉｃａＦｏｃｋ）全草中分离出６个化合物，其中，２个为新化合物，分别命名为蛇莓甙Ａ（ｄｕｃｈｅｓｉｄｅＡ）和蛇莓甙Ｂ（ｄｕｃｈｅｓｉｄｅＢ），经化学及光谱（ＵＶ，１Ｒ，１ＨＮＭＲ，１３ＣＮＭＲ，ＮＯＥＤＳ和ＭＳ）分析确定，蛇莓甙Ａ的结构为３＇－Ｏ－甲基－鞣花酸－４－Ｏ－β－Ｄ－吡喃木糖甙（Ⅰ），蛇莓甙Ｂ的结构为３＇－Ｏ－甲基－鞣花酸－４－Ｏ－α－Ｌ－呋喃阿拉伯糖甙（Ⅱ）。另外４个化合物为已知三萜类化合物：３β－羟基－乌苏烷－１２－烯－２８－羧酸（Ⅲ），２α，３β，１９α－三羟基－乌苏烷－１２－烯－２８－羧酸（Ⅳ），２α，３β，１９α－三羟基－乌苏烷－１２－烯－２８－羧酸－２８－Ｏ－β－Ｄ－吡喃葡萄糖甙（Ⅴ），２α，３α，１９α－三羟基－乌苏烷－１２－烯－２８－羧酸－２８－Ｏ－β－Ｄ－吡喃葡萄糖甙（Ⅵ）。其中化合物Ⅳ，Ⅴ和Ⅵ为首次从该属植物中分离得到。     

早开堇菜的有效成分(英文)

首次报道了自堇菜科植物早开堇菜（ＶｉｏｌａｐｒｉｏｎａｎｔｈａＢｇｅ．）全草中分离到三个香豆素类化合物。根据理化性质与光谱数据（ＩＲ．ＭＳ．１ＨＮＭＲ．１３ＣＮＭＲ．２ＤＮＭＲ）推定，化合物Ⅲ的结构为６－羟基，７－［（６－Ｏ－乙酰基－β－Ｄ－葡萄糖基）－Ｏ－］－香豆素［６－ｈｙｄｒｏｘｙ，７－［（６’－Ｏ－ａｃｅｔｙｌ－β－Ｄ－ｇｌｕｃｏｐｙｒａｎｏｓｙｌ）－ｏｘｙ－］－ｃｏｕｍａｒｉｎ］是新化合物，命名为早开堇菜甙（ｐｒｉｏｎａｎｔｈｏｓｉｄｅ）。化合物Ⅰ、Ⅱ分别为已知化合物七叶内酯（ｅｓｃｕｌｅｔｉｎ）和菊艺甙（ｃｉｃｈｏｒｉｉｎ）。     

金不换山酮成分的研究Ⅲ一个新山酮甙的结构测定

从民族药金不换（ＶｅｒａｔｒｉｌｌａｂａｉｌｌｏｎｉｉＦｒａｎｃｈ）根中分离到多种酮化合物，经化学及光谱法测定，其中之一为２，３，４，７－四甲氧基酮－１－Ｏ－β－Ｄ－木糖基（１→６）－β－Ｄ－葡萄糖甙（Ⅰ），是新的酮甙。     

女贞子化学成分的研究(Ⅱ)

继前报，作者又从女贞子（ＬｉｇｕｓｌｒｕｍＬｕｃｉｄｕｍＡｉｌ．）乙醇提取物的石油醚萃取部分和乙酸乙酯萃取部分分离鉴定了４个化合物．化合物Ⅵ为１９α－羟基－３－乙酰乌索酸（１９α－ｈｙｄｒｏｘｙ－３－ａｃｅｔｙｌ－ｕｒｓｏｌｉｃａｃｉｄ），化合物Ⅸ为对羟基苯乙醇－β－Ｄ－葡萄糖甙（ｐ－ｈｙｄｒｏｘｙｐｈｅｎｅｔｈｙｌ－β－Ｄ－ｇｌｕｃｏｓｉｄｅ），化合物Ｘ为芹菜素－７－Ｏ－β－Ｄ－葡萄糖甙（ｃｏｓｍｏｓｓｉｎ），化合物Ⅺ为甘露醇（ｍａｎｎｉｔｏｌ）．其中化合物Ⅵ为一新天然产物．     

东北淫羊藿活性成分研究

自东北淫羊藿（ＥｐｉｍｅｄｉｕｍｋｏｒｅａｎｕｍＭａｋａｉ）的地上部分，分离得到４个单体化合物，根据理化数据和光谱分析鉴定为：金丝桃甙（ｈｙｐｅｒｉｎ）（１），淫羊藿甙－Ⅱ（ｉｃａｒｉｓｉｄⅡ）（２）．淫羊藿甙－Ⅰ（ｉｃａｒｉｓｉｄⅠ）（３）和淫羊藿甙（ｉｃａｒｉｉｎ）（４）．其中，金丝桃甙在本种植物中首次分离得到，同时，我们又利用１Ｈ－１Ｈｃｏｓｙ和１３Ｃ－１Ｈｃｏｓｙ谱，对原文献中化合物２部分Ｃ信号的归属进行了修正，另外，药理实验结果表明：淫羊藿甙（ｉｃａｒｒｉｎ）对氢化可的松造成的小鼠阳虚有一定的拮抗作用．     

A New Phenylethanoid Glucoside from Buddleia officinalis

从密蒙花（Ｂｕｄｄｌｅｉａｏｆｆｉｃｉｎａｌｉｓ）中分离得到一个新苯乙醇甙，通过波谱分析，确定新化合物结构为β（３′，４′二羟基，６′Ｎ吡啶盐酸盐基苯基）乙基ＯαＬ吡喃鼠李糖基（１→３）βＤ（４″Ｏ咖啡酰基）呋喃葡萄糖甙，命名其为密蒙花新甙Ｂ。     

耳草属植物的化学研究I.黄毛耳草中环烯醚萜甙的分离和鉴定

从中药黄毛耳草（Ｈｅｄｙｏｔｉｓｃｈｒｙｓｏｔｒｉｃｈａ）中分离鉴定出１０个环烯醚萜类化合物，其中８个已知物为：车叶草甙（ａｓｐｅｒｕｌｏｓｉｄｅ，１），鸡屎藤甙甲酯（ｓｃａｎｄｏｓｉｄｅｍｅｔｈｙｌｅｓｔｅｒ，２），车叶草酸（ａｓｐｅｒｕｌｏｓｉｄｉｃａｃｉｄ，３），去乙酰车叶草酸（ｄｅａｃｅｔｙｌａｓｐｅｒｕｌｏｓｉｄｉｃａｃｉｄ，４），马钱子素（ｌｏｇａｎｉｎ，５），去乙酰车叶草甙（ｄｅａｃｅｔｙｌａｓｐｅｒｕｌｏｓｉｄｅ，６），乙酰鸡屎藤甙甲酯（ａｃｅｔｙｌｓｃａｎｄｏｓｉｄｅｍｅｔｈｙｌｅｓｔｅｒ，７），６β羟基京尼平（６βｈｙｄｒｏｘｙｇｅｎｉｐｉｎ，８）；２个新化合物命名为耳草甙（ｈｅｄｙｏｓｉｄｅ，９），６′乙酰车叶草甙（６′ａｃｅｔｙｌａｓｐｅｒｕｌｏｓｉｄｅ，１０）。除车叶草甙外均为首次从该植物得到，６β羟基京尼平为首次从天然界发现     

女贞子化学成分的研究（Ⅱ）1）

继前报，作者又从女贞子（Ｌｉｇｕｓｔｒｕｍ Ｌｕｃｉｄｕｍ Ａｉｔ。）乙醇提取物的石油醚萃取部分和乙酸乙酯萃取部分分离鉴定了４个化合物。化合物Ⅵ为１９ａ－羟基－３－乙酰乌索酸（１９ａ－ｈｙｄｒｏｘｙ－３－ａｃｅｔｙｌ－ｕｒｓｏｌｉｃ ａｃｉｄ），化合物Ⅸ为对羟基苯乙醇－β－Ｄ－葡萄糖甙（ｐ－ｈｙｄｒｏｘｙｐｈｅｎｅｔｈｙｌ－β－Ｄ－ｇｌｕｃｏｓｉｄｅ），化合物Ｘ为芹菜素－７－Ｏ－β－Ｄ－葡萄糖甙（     

The Chemical Constituents from the Roots of Bupleurum chinense DC.

从北柴胡（ＢｕｐｌｅｕｒｕｍｃｈｉｎｅｎｓｅＤＣ．）根的乙醇提取液中分离得到五个化合物，经理化性质及波谱解析分别鉴定为水仙甙（１）、腺苷（２），尿苷（３）、ａ菠甾醇葡萄糖甙（４）及木糖醇（５）。均为首次从北柴胡中分离得到。     

Two new anthraquinone glycosides from the roots of Rheum palmatum

Two new anthraquinone glycosides, named 1-methyl-8-hydroxyl-9,10-anthraquinone-3-O-β-D-(6'-O-cinnamoyl)glucopyranoside (1) and rhein-8-O-β-D-[6'-O-(3'-methoxyl malonyl)]glucopyranoside (2), have been isolated from the roots of Rheum palmatum, together with seven known compounds, rhein-8-O-β-D-glucopyranoside (3), physcion-8-O-β-D-glucopyranoside (4), chrysophanol-8-O-β-D-glucopyranoside (5), aleo-emodin-8-O-β-D-glucopyranoside (6), emodin-8-O-β-D-glucopyranoside (7), aleo-emodin-ω-O-β-D-glucopyranoside (8), and emodin-1-O-β-D-glucopyranoside (9). Their structures were elucidated on the basis of chemical and spectral analysis.     

藤梨根化学成分的研究

A number of constituents were isolated from the root of Actinidia chinensis Planch. Seven of them were indentified as physcion (emodin-6-methyl ether) (T₁), emodin (frangala-emodin) (T₂), emodin-8-β-D-glucoside (T₃), questin (emodin-8-methyl ether) (T₅), citreorosein (ω-hydroxyemodin) (T₆), emodic acid (T₇) and β-sitosterol (T₄).Comparison of the ¹HNMR data of emodin (T₂) and its 8-glucoside (T₃) shows that the aromatic proton experiences a downfield shift of about 0.5 ppm upon glycosidation of an ortho phenolic group, a fact which can be used to advantage for the allocation of the glycosidic linkage.     

白蔹的化学成分(Ⅱ)

目的研究白蔹的化学成分。方法利用各种色谱法进行分离,根据理化性质和波谱分析鉴定化合物结构。结果从白蔹的氯仿和乙酸乙酯萃取部分又分离得到7个已知成分,分别鉴定为五味子苷(1)、大黄酚(2)、大黄素甲醚(3)、大黄素-8-O-β-D-吡喃葡萄糖苷(4)、poriferast-5-en-3β,7α-diol(5)、棕榈酸(6)、齐墩果酸(7)。结论化合物4和5为首次从该植物中分离得到。     

Comparative analyses of chromatographic fingerprints of the roots of Polygonum multiflorum Thunb. and their processed products using RRLC/DAD/ESI-MS(n)

The dried roots of Polygonum multiflorum Thunb. (Heshouwu) and their processed products (Zhi-heshouwu) are widely used in traditional Chinese medicine, yet their therapeutic effects are different. Previous investigations focused mainly on the differences between Heshouwu and Zhi-heshouwu in the contents of several known compounds. In this study, a rapid resolution liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (RRLC/DAD/ESI-MS(n)) method was developed for the comparative analysis of the components of Heshouwu and Zhi-heshouwu. A total of 23 compounds were identified or tentatively characterized. We found that 16 batches of Heshouwu and 15 batches of Zhi-heshouwu samples shared eight compounds, including gallic acid; 3,5,4'-tetrahydroxylstilbene-2,3-di-O-glucoside, CIS-2,3,5,4'-tetrahydroxylstilbene-2-O- β-D-glucoside, trans-2,3,5,4'-tetrahydroxylstilbene-2-O- β-D-glucoside, emodin-8-O- β-D-glucoside, physcion-8-O- β-D-glucoside, emodin, and physcion. Nevertheless, the relative amounts of gallic acid, emodin, and physcion were very high in Zhi-heshouwu samples compared to those in Heshouwu samples. Six compounds disappeared after processing and were unique for Heshouwu: catechin, flavanol gallate dimer, polygonimitin B, emodin-1-O-glucoside, emodin-8-O-(6'-O-malonyl)-glucoside, and physcion-8-O-(6'-O-malonyl)-glucoside. Three compounds were unique for Zhi-heshouwu: hydroxymaltol, 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one, and 5-hydroxymethyl furfural. These results suggest that the types and relative amounts of the chemical components of Heshouwu and Zhi-heshouwu are different.     

白蔹的化学成分研究(Ⅱ)

目的 研究白蔹的化学成分。方法 利用各种色谱法进行分离,根据理化性质和波谱分析鉴定化合物结构。结果 从白蔹的氯仿和乙酸乙酯萃取部分又分离得到7个已知成分,分别鉴定为五味子苷（1）、大黄酚（2）、大黄素甲醚（3）、大黄素- 8-0-β-D-吡喃葡萄糖苷（4）、poriferast-5-en-3β,7α-diol（5）、棕榈酸（6）、齐墩果酸（7）。结论 化合物4和5为首次从该植物中分离得到。     

黄药的化学成分研究

目的研究黄药(Rhamnus crenatus)的化学成分。方法用硅胶正相色谱及聚酰胺色谱技术进行分离纯化，根据理化性质、光谱数据进行结构鉴定。结果从黄药地上部分的95%乙醇提取物的乙酸乙酯部分中分离得到了5个化合物：黄药苷(1)、torachrysone (2)、大黄素(3)、大黄素-1-O-β-D-葡糖苷(4)和β-谷甾醇(5)。结论化合物1为新化合物，其余化合物均为首次从该植物中分离得到。     

首乌藤的化学成分

目的分离和鉴定首乌藤中的化学成分。方法利用硅胶柱色谱、Sephadex LH-20柱色谱法等进行分离纯化,并通过理化常数测定和光谱分析鉴定了其化学结构。结果分离得到9个化合物,分别鉴定为:大黄素甲醚(physcion,1)、大黄素-8-甲醚(questin,2)、2,5-二甲基-7-羟基色原酮(2,5-dimethyl-7-hydroxychromone,3)、大黄素(emodin,4)、儿茶素(catechin,5)、大黄素甲醚-8-O-β-D-葡萄糖苷(physcion-8-O-β-D-glucopyranoside,6)、决明蒽酮-8-O-β-D-葡糖苷(torachrysone-8-O-β-D-glucopyranoside,7)、大黄素甲醚-8-O-β-D-葡萄糖苷(emodin-8-O-β-D-glucopyranoside,8)、2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(2,3,5,4′-tetrahydroxystil-bene-2-O-β-D-glucopyranoside,9)。结论化合物3、7为该种植物中首次分离得到的化合物。     

Lipoxygenase inhibitory constituents from rhubarb

Phytochemical study on the ethanol extract of rhubarb led to the isolation of fifteen compounds, including five anthraquinones: chrysophanol (1), physcion (2), emodin (7), chrysophanol-8-O-beta-D: -glucopyranoside (9) and emodin-8-O-beta-D: -glucopyranoside (15), and ten stilbenes: desoxyrhaponticin (3), rhaponticin (4), resveratrol (5), desoxyrhapotigenin (6), rhapontigenin (8), piceatannol-3'-O-beta-D: -glucopyranoside (10), piceid (11), epsilon-viniferin (12), ampelopsin B (13) and isorhaponticin (14). Their structures were identified by comparing the physicochemical data with those of published papers. Among the isolated compounds, stilbene derivatives (3-6, 8 and 10-14) showed remarkable inhibitory effect on lipoxygenase with IC(50) values ranging from 6.7 to 74.1 microM. The inhibition kinetics analyzed by Lineweaver-Burk plots found that they were competitive inhibitors with the linoleic acid at the active site of lipoxygenase. In addition, stilbenes exhibited significantly free radical scavenging activity against ABTS(.+) with trolox equivalent activity capacity (TEAC) values ranging from 1.16 to 4.64. Whereas, anthraquinone derivatives (1-2, 7, 9 and 15) neither inhibited lipoxygenase nor scavenged free radical ABTS(.+). These results indicated that stilbene derivatives were considerate to be mainly lipoxygenase inhibitor and free radical scavenger constituents of rhubarb.     

塔黄的化学成分研究

目的对塔黄的化学成分进行研究。方法通过各种柱色谱对其成分进行分离纯化,通过波谱解析进行结构鉴定。结果从中分离得到8个化合物,分别鉴定为:大黄素(1),大黄酚(2),大黄素甲醚(3),1,8-二羟基-2,3-甲氧基-6-甲基蒽醌(4),大黄酚葡萄糖苷(5),大黄素葡萄糖苷(6),β-谷甾醇(7),胡萝卜素(8)。结论所有化合物均为首次从该植物中分离得到。     

Neuroprotective effects of emodin-8-O-beta-D-glucoside in vivo and in vitro

Emodin-8-O-beta-D-glucoside extracted from the traditional Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc is widely used to treat acute hepatitis possibly by antioxidative mechanisms. The present study was designed to investigate whether emodin-8-O-beta-D-glucoside exerted neuroprotective effects on the focal cerebral injury induced by ischemia and reperfusion in vivo and on the neuronal damage induced by glutamate in vitro, and to study the possible mechanisms. Male Wistar rats were used to establish the model of ischemia and reperfusion. The behavioral test was performed and the cerebral infarction area was assessed in the brain slices stained with 2% 2,3,5-triphenyl tetrazolium chloride to evaluate the neuroprotective effects of emodin-8-O-beta-D-glucoside. Superoxide dismutase (SOD) activity, total antioxidative capability and malondialdehyde (MDA) level in the brain tissue were determined with spectrophotometrical methods to probe the primary mechanisms of emodin-8-O-beta-D-glucoside. In vitro, the neuroprotective effects of emodin-8-O-beta-D-glucoside were tested in the cultured cortical cells of fetal rats exposed to glutamate. Emodin-8-O-beta-D-glucoside concentration in plasma and brain tissue was also measured to examine distribution of emodin-8-O-beta-D-glucoside in the brain. The results showed that the treatment of rats with emodin-8-O-beta-D-glucoside reduced the neurological deficit score and the cerebral infarction area, increased SOD activity and total antioxidative capability, and decreased MDA level in the brain tissue in dose-dependent way. Emodin-8-O-beta-D-glucoside also inhibited the neuronal damage induced by glutamate. Besides, emodin-8-O-beta-D-glucoside was able to penetrate blood-brain barrier and distribute in the brain tissue. These findings demonstrate that emodin-8-O-beta-D-glucoside is able to provide neuroprotection against cerebral ischemia-reperfused injury and glutamate induced neuronal damage through exerting antioxidative effects and inhibiting glutamate neurotoxicity.