细胞外基质金属蛋白酶诱导因子与牙周炎的关系

细胞外基质金属蛋白酶诱导因子广泛地存在于人类各种肿瘤细胞和一些正常细胞中，能刺激基质金属蛋白酶的合成，促进细胞外基质的降解，在恶性肿瘤的浸润和转移、风湿性关节炎、心血管疾病、糖尿病和牙周炎的发生和发展中起着重要的作用。本文将就细胞外基质金属蛋白酶诱导因子及其与牙周炎关系的最新研究进展作一综述。     

细胞外基质金属蛋白酶诱导因子与牙周炎的关系

细胞外基质金属蛋白酶诱导因子广泛地存在于人类各种肿瘤细胞和一些正常细胞中,能刺激基质金属蛋白酶的合成,促进细胞外基质的降解,在恶性肿瘤的浸润和转移、风湿性关节炎、心血管疾病、糖尿病和牙周炎的发生和发展中起着重要的作用。本文将就细胞外基质金属蛋白酶诱导因子及其与牙周炎关系的最新研究进展作一综述。     

细胞外基质金属蛋白酶诱导因子与恶性肿瘤

细胞外基质金属蛋白酶诱导因子广泛地存在于人体各种肿瘤组织中诱导基质金属蛋白酶的产生,从而促进肿瘤的侵袭并在恶性肿瘤的生长、浸润、转移和诱导肿瘤组织的恶性潜能等方面起重要作用.下面就细胞外基质金属蛋白酶诱导因子的作用机制、调节以及与口腔颌面部肿瘤的关系作一.     

舌鳞癌组织细胞外基质金属蛋白酶诱导因子的表达及其与预后的关系

目的：研究舌鳞癌组织中细胞外基质金属蛋白酶诱导因子（EMMPRIN）的表达及其与舌癌患者临床病理特征和生存期之间的相关性。方法：应用SP免疫组织化学法,检测68例舌鳞癌组织及相应的癌旁组织中EMMPRIN的表达情况,并对68例舌鳞癌患者进行随访观察。应用非参数秩和检验检测两个独立样本的EMMPRIN的表达差异,应用SPSS 13.0软件包进行生存分析;应用Cox比例风险模型分析预后。结果：EMMPRIN在舌鳞癌组织中的表达阳性率高于相应的癌旁组织（P〈0.05）。舌鳞癌组织中EMMPRIN的表达与肿瘤大小和临床分期密切相关,与性别、年龄、淋巴结转移和肿瘤病理分化程度不相关。Cox比例风险模型多因素预后分析显示,EMMPRIN表达是影响舌鳞癌患者预后的独立因素。结论：EMMPRIN可以作为舌鳞癌预后判断的参考指标,并有望成为口腔癌生物治疗的潜在靶点。     

基质金属蛋白酶与头颈肿瘤的侵袭和转移

肿瘤的侵袭与转移是恶性肿瘤的主要生物学特征 ,也是恶性肿瘤患者的主要死因。从原位的增殖性肿瘤到侵袭转移癌的演变过程中 ,基质金属蛋白酶 (ｍａｔｒｉｘｍｅｔａｌｌｏｐｒｏｔｅｉｎａｓｅｓ ,ＭＭＰｓ)起到重要作用 ,它通过破坏基质的降解平衡而促进肿瘤细胞突破基底膜和细胞外基质 (Ｅｘｔｒａｃｅｌｌｍａｔｒｉｘ ,ＥＣＭ )构成的组织学屏障 ,向周围组织侵袭和远处组织转移。因此 ,ＭＭＰｓ近年已成为肿瘤侵袭和转移研究的热点。1　ＭＭＰｓ简介ＭＭＰｓ也称为基质素 ,此家族是一组Ｚｎ2 +依赖性蛋白酶 ,目前所知道脊椎动物的ＭＭ…     

基质金属蛋白酶与牙周膜细胞外基质代谢

基质金属蛋白酶在牙周膜细胞外基质代谢过程中发挥着关键性的调节作用，而牙周膜细胞外基质降解与合成的平衡是牙周组织改建的物质基础。本文就基质金属蛋白酶的一般特性、活性调节及对牙周膜细胞外基质代谢的影响进行了综述。     

基质金属蛋白酶与牙周膜细胞外基质代谢

基质金属蛋白酶在牙周膜细胞外基质代谢过程中发挥着关键性的调节作用,而牙周膜细胞外基质降解与合成的平衡是牙周组织改建的物质基础。本文就基质金属蛋白酶的一般特性、活性调节及对牙周膜细胞外基质代谢的影响进行了综述。     

基质金属蛋白酶在口腔鳞癌侵袭和转移中的作用

基质金属蛋白酶在癌细胞侵袭和转移过程中，通过破坯基质的降解平衡。本文就ＭＭＰｓ的活性机理及其在腔鳞癌侵袭转移中的作用进行综述。     

基质金属蛋白酶及其抑制剂与口腔肿瘤侵袭转移过程的研究进展

基质金属蛋白酶(MMPs)及其抑制物(TIMPs)在口腔肿瘤的侵袭转移过程中发挥重要作用,本文就其近年来的有关研究进展作一综述.     

基质金属蛋白酶在牙齿发育及牙源性肿瘤中的研究进展

不同的基质金属蛋白酶(MMP)在不同时空参与了牙体组织形成和矿化,它们各自的作用不同,机制各异;同时彼此间又相互作用,并受到其他因素的调节。MMPs对细胞外基质的降解作用和牙源性肿瘤的发生、侵润和转移密切相关。     

The temporal expression and localization of extracellular matrix metalloproteinase inducer (EMMPRIN) during the development of perio‐dontitis in an animal model

Liu L, Li C, Cai X, Xiang J, Cao Z, Dong W. The temporal expression and localization of extracellular matrix metalloproteinase inducer (EMMPRIN) during the development of periodontitis in an animal model. J Periodont Res 2010; 45: 541–549. © 2010 John Wiley & Sons A/S Background and Objective: We previously demonstrated extracellular matrix metalloproteinase inducer (EMMPRIN) was associated with the matrix metalloproteinases production of human periodontitis. The aim of this study was to investigate the temporal expression and localization of EMMPRIN during ligature‐induced periodontitis in rats. Material and Methods: Periodontitis was inducd in rats by placing a thread around the cervix of the first mandibular molar. Animals were killed 3, 7, 11, 15 or 21 d after ligation. Mandibles were processed for paraffin sections and stained with hematoxylin and eosin or picrosirius red. The distance from the amelocemental junction to the alveolar crest (ACJ–AC) and the area fraction (Area%) of collagen fibers were measured. EMMPRIN was examined by immunohistochemistry and quantified by positive cell counting. Correlation analyses were then performed. Results: Histologically, alveolar bone was gradually destroyed from day 3 to 11 and then stabilized. Collagen fibers were slightly dissociated on day 3 and extensively broken on day 7. They were reconstructed from day 11 to 21. EMMPRIN was localized predominantly in infiltrating cells and adjacent fibroblasts in interdental gingiva. The number of EMMPRIN‐positive cells increased on day 3, peaked on day 7 and then gradually subsided from day 11 to 21. Statistically, there was a moderate positive correlation regarding the ACJ–AC distance (r = 0.552, p < 0.01) and a strong negative correlation with the Area% of collagen fibers (r = ?0.808, p < 0.01). In gingival epithelium, the immunoreactivity was extremely strong in basal layer cells and sulcular epithelial cells in health. It was greatly enhanced in the inflamed conditions on days 3 and 7. In the interradicular bone, EMMPRIN was localized in the osteoclasts on days 3 and 7, as well as in the osteoblasts from day 11 onwards. Conclusion: The expression and localization of EMMPRIN are temporally varied during the development of periodontitis. In addition, the inflammation‐dependent expression of EMMPRIN might be involved in alveolar bone resorption and collagen breakdown.     

细胞外基质与涎腺腺样囊性癌的远处转移

目的 研究涎腺腺样囊性癌与细胞外基质的关系。方法 通过肿瘤手术标本的免疫组化染色和超微结构观察、细胞系体外粘附实验及人工重组基底膜侵袭实验，并采用整合素受体抑制剂精氨酸-天冬氨酸进行体外及荷瘤动物体内实验，观察其预防及治疗肺转移的效果。结果 肿瘤团索周围的基膜样物质呈多层、溶解或断裂现象。出现腺样囊性癌远处转移患者的肿瘤标本组织蛋白酶D免疫组化阳性反应率(75％)明显高于无转移患者(43．8％)。肺高转移涎腺腺样囊性癌细胞株对人工重组基膜的侵袭细胞数明显高于其相应的非高转移细胞系。精氨酸-天冬氨酸在体外能抑制肺高转移涎腺腺样囊性癌细胞对人工重组基膜的侵袭，在体内能显著延长涎腺腺样囊性癌实验性肺转移动物的生存期。结论 涎腺腺样囊性癌的远处转移与细胞外基质有密切关系。     

Expression of extracellular matrix metalloproteinase inducer and enhancement of the production of matrix metalloproteinase-1 in tongue squamous cell carcinoma

Recent studies have found that in addition to promoting cellular invasion, overexpression of metalloproteinase -1 (MMP-1) is associated with the initial stages of cancer development. Extracellular matrix metalloproteinase inducer (EMMPRIN), a transmembrane glycoprotein, has been reported to be highly expressed in tumor cells and induce production of MMPs from peritumor fibroblasts (PTFs) adjacent to the tumor cells. The expression of EMMPRIN in tongue squamous cell carcinoma (SCC) was investigated in this study. It was found that EMMPRIN was expressed at the cell membrane throughout the entire lesion in tongue SCC. Immunofluorescence staining localized EMMPRIN to the cell membrane in a highly invasive tongue SCC cell line (Tca 8113). EMMPRIN mRNA was expressed at a high level in Tca 8113, whereas MMP-1 mRNA was expressed in PTF but harder to be detected in Tca 8113. Co-culture of Tca 8113 with PTF stimulated production of MMP-1. EMMPRIN was highly expressed in tongue SCC, and could induce local production of MMP-1. These data indicate that EMMPRIN might play an important role in tongue SCC progression and invasion.     

Association of matrix metalloproteinase inducer (EMMPRIN) with the expression of matrix metalloproteinases-1, -2 and -9 during periapical lesion development

Objective

To evaluate the expression of matrix metalloproteinase inducer (EMMPRIN) and its correlation with the expression of matrix metalloproteinases (MMPs)-1, -2 and -9 during the development of periapical lesion in mice.

Methods

Periapical lesions were induced in the lower first molars of mice and after 7, 14, 21 and 42 days the mandibles were removed. The periapical lesions were measured by micro-computed tomography. The expression of EMMPRIN, MMPs-1, -2, and -9 genes were determined by real-time RT-PCR. The location and expression of EMMPRIN and MMPs were evaluated by immunohistochemistry.

Results

At 14 days, the periapical lesion area was higher than at 7 days. At 21 and 42 days no statistically significant bone loss was observed in comparison to 14 days. The control group showed discrete and occasional EMMPRIM, MMP-1, -2 and -9 immunostaining in the periodontal ligament fibroblasts. At 7, 14, 21 and 42 days intense immunoexpression was observed for EMMPRIN, MMPs-1, -2 and -9 in the region adjacent to the apical foramen. The EMMPRIN immunoexpression was higher at 7, 14, 21 and 42 days compared with the control. There was a positive correlation between gene expression of EMMPRIN and MMPs in the active phase of periapical lesion development.

Conclusion

There is a high expression of EMMPRIM mainly by the inflammatory infiltrate in the region adjacent to the apical foramen during periapical lesion development. Furthermore, the positive correlation with MMP-1, -2, and -9 during the first days after periapical lesion induction indicates that EMMPRIM may be involved in the active phase of periapical lesions development.     

盘状结构域受体1与基质金属蛋白酶2在唾液腺腺样囊性癌中的表达及意义

目的:检测唾液腺腺样囊性癌(salivary gland adenoid cystic carcinoma, SACC）组织中盘状结构域受体1（discoidin domain receptor 1,DDR1）与基质金属蛋白酶2（matrix metalloproteinase 2,MMP2）的表达,探讨两者在SACC中的表达意义及相关性。方法:应用SP免疫组织化学方法检测54例SACC标本和30例正常唾液腺组织中DDR1与MMP2的表达情况。采用SPSS13.0软件包对数据进行统计学分析。结果:DDR1在SACC中的阳性表达率为87.0%,显著高于正常唾液腺组织（10%,P<0.01）。DDR1的高表达与SACC的神经侵袭、淋巴结转移相关（P<0.05）。MMP2在SACC中的阳性表达率为68.5%,显著高于正常唾液腺组织（13.3%,P<0.01）。经Spearman等级相关分析,DDR1与MMP2呈现显著正相关（r=0.332, P<0.05）。结论:在SACC中,DDR1与MMP2共同高表达,与SACC的发生与发展相关。     

金属蛋白酶及其组织抑制剂在涎腺腺样囊性癌细胞株表达的分子生物学研究

为了研究金属蛋白酶（ｍｅｔａｌｏｐｒｏｔｅｉｎａｓｅ，ＭＭＰ）及其组织抑制剂（ｔｉｓｓｕｅｉｎｈｉｂｉｔｏｒｏｆｍｅｔａｌｏｐｒｏｔｅｉｎａｓｅ，ＴＩＭＰ）对涎腺腺样囊性癌细胞（ａｄｅｎｏｉｄｃｙｓｔｉｃｃａｒｃｉｎｏｍａ，ＡＣＣ）转移的影响，本研究应用斑点印迹杂交的分子生物学方法检测ＡＣＣ２细胞系及高转移细胞株ＡＣＣＭ中ＭＭＰ２、ＭＭＰ９和ＴＩＭＰ２的表达。结果显示ＭＭＰ２，ＭＭＰ９在ＡＣＣＭ表达较高，在ＡＣＣ２表达较低，而ＴＩＭＰ２在ＡＣＣ２表达较高，在ＡＣＣＭ表达较低，证实ＭＭＰ２，ＭＭＰ９促进转移的发生，而ＴＩＭＰ２则抑制转移的发生。提示ＭＭＰ与ＴＩＭＰ平衡关系可能是ＡＣＣ转移的关键机制之一。