Guanylate cyclase activity was examined in the choroid plexus and isolated cellular fractions including crude synaptosomes of the rat cerebral cortex. Under conditions of low Mn2+ (0.5 mM) the enzyme activity was depressed in preparations (homogenates, 100,000 g soluble and paniculate fractions) of cerebral cortex, pia-arachnoid, choroid plexus and cerebral neuronal (perikaryal or capillary fractions. With the homogenate and soluble enzyme forms, addition of Ca2+elicited an activation of guanylate cyclase in all preparations except glia. Moreover, the neuronal and cortical paniculate fractions were responsive to Ca2+. The calcium-dependent regulator protein (calmodulin) and carbamyl choline were without stimulatory actions on any guanylate cyclase preparation. The preparations most sensitive to activation by sodium azide consisted of those containing neuronal elements, i.e. the crude synaptosomal homogenate, the paniculate neuronal fraction, and the total cortex (homogenate and particulate). Using the homogenates as a basis for comparing basal enzyme activities, the highest guanylate cyclase activity was observed in the pia-arachnoid > total cortex > choroid plexus > synaptosomes, glia > neurons >capillaries.Thus guanylate cyclase is widely distributed among the different cellular types in the brain and individual cells vary in their sensitivities to Ca2+, azide and hydroxylamine. 相似文献
Bifunctional couplers based on carbonate chemistry – Series A, B, and C – that are able to combine molecules bearing amine groups via substitution and addition reaction (Series A) or only via addition (Series B and C) were synthesized and characterized. The reactivity and selectivity of the two functional groups of couplers toward primary amines in different conditions were studied. The reactivity of couplers of Series B towards secondary and tertiary amines was investigated. The effect of adjacent groups (α/δ‐position to O/N sides of urethane groups or α‐position to ether groups) on the reactivity of the five‐membered cyclic carbonates was studied using model compounds 4 , 5 , 13 , and 16 .
We investigated in vitro accumulation of adenosine 3,5-monophosphate (induced by 5-hydroxytryptamine and dopamine) and of guanosine 3,5-monophosphate (induced by acetylcholine) in the anterior byssus retractor muscle ofMytilus.The response to 5-hydroxytryptamine exceeded that induced by equimolar concentrations of dopamine.1-methyl lysergic acid, a 5-hydroxytryptamine-blocking agent, diminished the 5-hydroxytryptamine-induced increase of cyclic AMP level. This parallels the effect of this amine on the contracted muscle.Acetylcholine, which causes a tonic contraction of the muscle, increased intracellular levels of cyclic GMP in a dose-dependent (max. 45-fold at 10–4 M ACh) manner. The time course of the rise in cyclic GMP level was rapid and transient (peak concentration of cyclic GMP at 2 min).Mytolon was the most effective of all cholinergic blockers tested. It was concluded that cyclic nucleotides may play a role in the modulatory process of the transmitters. A direct relation to the relaxation-contraction process could not be established.Abbreviations ABRM
anterior byssus retractor muscle
- cyclic AMP
adenosine 3,5-monophosphate
- cyclic GMP
guanosine 3,5-monophosphate
- ACh
acetylcholine
- 5-HT
5-hydroxytryptamine, serotonin
- DA
dopamine
- TCA
trichloroacetic acid
- UML
1-methyl lysergic acid hydrogen maleinate 相似文献
Cyclic olefin copolymer (COC) polymers with rigid ring structures like NB in the polymer chain enhance the toughness greatly, but also cause high brittleness of the material, which limits its application. Modification of COC for toughness by the addition of styrene-butadiene-styrene block copolymer (SBS) and polystyrene-block-poly(ethene-co-butyl-1-ene)-block-polystyrene (SEBS) elastomers of styrene content in SEBS is done. Field emission scanning electron microscope shows that tougheners are uniformly dispersed in the COC matrix without a visible interface between the two phases, which is also supported by the Cole-Cole and Han curves. Rheological behavior indicates that the addition of SBS and SEBS makes the COC/SBS/SEBS composites to have higher elastic modulus, loss modulus, and composite viscosity than that of pure COC. Furthermore, the tensile strength of the composite presents 3.6 times higher than pure COC, while the elongation at break is 6.3 times higher than pure COC. The dynamic mechanical analysis reveals that the COC and tougheners are well blended, and the SBS and SEBS toughen the COC in agreement with the results of morphological and rheological behaviors. 相似文献