狼毒大戟化学成分研究

目的研究狼毒大戟化学成分。方法采用硅胶柱层析进行分离纯化化合物,根据理化性质和现代波谱技术进行结构鉴定。结果从狼毒大戟根的石油醚部分分离出5个化合物,分别鉴定为2,4-二羟基-6-甲氧基-3-甲基苯乙酮(1)、3,3′-二乙酰基-4,4′-二甲氧基-2,2′,6,6′-四羟基二苯甲烷(2)、2,4-二羟基-3-醛基-6-甲氧基苯乙酮(3)、富马酸(4)、β-谷甾醇(5)。结论5个化合物均为已知化合物,化合物3为首次从该植物中获得。     

生姜化学成分的研究

目的研究生姜Zingiber officinale的化学成分。方法采用乙醇提取、硅胶柱色谱、凝胶柱色谱进行分离和纯化,单体化合物用波谱分析进行结构鉴定。结果从醋酸乙酯部位分离得到12个化合物,鉴定为三十一烷醇(Ⅰ)、正二十四烷酸(Ⅱ)、β-谷甾醇(Ⅲ)、1-羟基-7-甲氧基酮(Ⅳ)、优酮(Ⅴ)、1,6-二羟基酮(Ⅵ)、8-姜酚(Ⅶ)、6-姜酚(Ⅷ)、6-姜烯酚(Ⅸ)、1-去氢姜辣二酮(Ⅹ)、3,5-二酮-1,7-二-(3-甲氧基-4-羟基)苯基庚烷(Ⅺ)、(3S,5S)-3,5-二羟基-1-(4-羟基-3-甲氧基苯基)癸烷(Ⅻ)。结论化合物Ⅰ～Ⅵ为首次从该植物中分离得到,化合物Ⅳ～Ⅵ为首次分离得到的酮类化合物。     

准噶尔大戟的化学成分研究

目的 对准噶尔大戟中的化学成分进行分离鉴定.方法 采用硅胶、Sephadex LH-20及MPLC制备色谱技术,通过理化方法和光谱分析鉴定其结构.结果 从准噶尔大戟的乙醇提取物中得到11个化合物,分别鉴定为香茅二醇(Ⅰ)、白桦脂醇(Ⅱ)、白桦脂酸(Ⅲ)、齐墩果酸(Ⅳ)、7-O-β谷甾醇(Ⅴ)、3-甲氧基-4-羟基反式苯丙烯酸正二十八醇酯(Ⅵ)、二十八酸(Ⅶ)、I-二十八烯(Ⅷ)、24-亚甲基-环阿尔廷醇(Ⅸ)、大戟醇(Ⅹ)、β-谷甾醇(Ⅺ).结论 化合物Ⅰ～Ⅷ均为首次从该植物中分离得到.     

金龙胆草化学成分及其抗溃疡活性研究(Ⅰ)

目的研究金龙胆草Conyza blinii的化学成分及其抗溃疡活性。方法采用溶剂提取、重结晶和硅胶、聚酰胺柱色谱方法分离纯化,根据氢谱、碳谱、质谱以及与对照品共薄层色谱鉴定化合物的结构。用幽门结扎大鼠模型进行抗溃疡活性筛选。结果从金龙胆草的乙醇提取物中分离鉴定了8个化合物:5,8,3′,4′-四羟基-7-甲氧基黄酮(Ⅰ)、5,8,4′-三羟基-7,3′-二甲氧基黄酮(Ⅱ)、β-谷甾醇(Ⅲ)、菠甾醇(Ⅳ)、正二十六烷酸(Ⅴ)、5,7-二羟基-3,3′,4′-三甲氧基黄酮(Ⅵ)、5,7-二羟基-3,8,4′-三甲氧基黄酮(Ⅶ)和苦蒿素(Ⅷ)。苦蒿素能明显减少模型大鼠胃溃疡面积,明显减少胃黏膜组织中的MDA的水平。结论化合物Ⅰ~Ⅲ和Ⅴ~Ⅶ为首次从该植物中分离得到。苦蒿素对幽门结扎大鼠胃溃疡的形成有明显抑制作用,其可能作用机制与抑制胃黏膜组织中MDA的生成有关。     

穿龙薯蓣地上部分的化学成分

目的研究穿龙薯蓣地上部分的化学成分。方法应用多种色谱技术进行分离纯化,根据化合物的理化性质和光谱数据分析鉴定其结构。结果得到10个化合物,分别鉴定为7-羟基-2,6-二甲氧基-1,4-菲醌(Ⅰ)、山柰酚-3-O-β-芸香糖苷(Ⅱ)、山柰酚-3-O-β-D-吡喃葡萄糖苷(Ⅲ)、(3S)-6,8-二羟基-3-苯基-3,4-二氢异香豆素(mon-troumarin,Ⅳ)、4′,5-二羟基-3,3′-二甲氧基联苄(Ⅴ)、薯蓣皂苷元(Ⅵ)、胡萝卜苷(Ⅶ)、β-谷甾醇(Ⅷ)、甘露醇(Ⅸ)、正癸烷(Ⅹ)。结论化合物Ⅰ~Ⅴ均为首次从该属植物中获得。     

铁皮石斛化学成分的研究

目的 研究铁皮石斛 Dendrobium candidum 水提物醋酸乙酯萃取物的化学成分。方法 采用硅胶柱色谱、ODS柱色谱、高效液相色谱等多种色谱学方法进行分离和纯化,根据波谱学特征鉴定化合物的结构。结果 共分离得到11个化合物,分别鉴定为(E)-对羟基桂皮酸(Ⅰ)、对羟基苯丙酸(Ⅱ)、松柏醇(Ⅲ)、香草醇(Ⅳ)、穆坪马兜铃酰胺(Ⅴ)、顺式阿 魏酸酰对羟基苯乙胺(Ⅵ)、反式桂皮酸酰对羟基苯乙胺(Ⅶ)、4′,5-二羟基3,3′-二甲氧基联苄(Ⅷ)、丁香脂素(Ⅸ)、4-烯丙基2,6-二甲氧基苯基葡萄糖苷(Ⅹ)和柚皮素(Ⅺ)。结论 化合物Ⅰ、Ⅳ～Ⅶ和Ⅹ为首次从石斛属植物中分离得到,所有化合物均为首次从铁皮石斛中分离得到。     

水菖蒲的化学成分研究

目的研究水菖蒲Acorus calamus的化学成分。方法应用硅胶、Sephadex LH-20等色谱技术进行分离纯化,采用MS、NMR等波谱技术解析结构。结果从水菖蒲中共分离得到9个化合物,分别鉴定为:表水菖蒲酮(ep ishyobunone,Ⅰ)、β-细辛醚(β-asarone,Ⅱ)、棕榈酸(palmiticacid,Ⅲ)、异水菖蒲酮(isoshyobunone,Ⅳ)、异水菖蒲二醇(isocalammendiol,Ⅴ)、β-谷甾醇(β-sitosterol,Ⅵ)、5-羟基-7,8,3′,4′-四甲氧基黄酮(5-hydroxy-7,8,3′,4′-tetram ethoxyflavone,Ⅶ)、5,4′-二羟基-7,8二-甲氧基黄酮(5,4′-dihydroxy-7,8-dimethoxyflavone,Ⅷ)、β-胡萝卜苷(β-daucosterol,Ⅸ)。结论化合物Ⅶ和Ⅷ为首次从菖蒲属植物中分离得到。     

刺桐化学成分的研究

目的研究刺桐E ry thrina varieg a ta茎皮的化学成分。方法采用硅胶、Sephadex LH-20柱色谱分离化合物,运用波谱技术分析确定化学结构。结果从刺桐茎皮65%乙醇提取物的醋酸乙酯萃取层中分离得到9个化合物,利用理化性质及波谱分析确定其结构分别为:5,4′-二羟基-8-(3,3-二甲基烯丙基)-2,″2″-二甲基吡喃[5,6∶6,7]异黄酮(Ⅰ)、豆甾醇(Ⅱ)、erythrinas inate B(Ⅲ)、3-羟基-2,′2′-二甲基吡喃[5,6∶9,10]紫檀烷(Ⅳ)、5,7-二羟基-8-(3,3-二甲基烯丙基)-二氢黄酮(Ⅴ)、5,4′-二羟基-2,″2″-二甲基吡喃[5,6∶6,7]异黄酮(Ⅵ)、5,7,4′-三羟基-6,8-二(3,3-二甲基烯丙基)异黄酮(Ⅶ)、5,2,′4′-三羟基-8-(3,3-二甲基烯丙基)-2,″2″-二甲基吡喃[5,6∶6,7]异黄酮(Ⅷ)、5,4′-二羟基-6-(3,3-二甲基烯丙基)-2,″2″-二甲基吡喃[5,6∶7,8]异黄酮(Ⅸ)。结论化合物Ⅰ、Ⅲ、Ⅳ、Ⅶ、Ⅸ为首次从该植物中分离得到,化合物Ⅴ为首次从该属植物中分离得到。     

红芪化学成分和抗肿瘤活性研究

目的 研究红芪Hedysarum polybotrys的化学成分和生物活性。方法 采用硅胶柱色谱,Sephadex LH-20分离化学成分,根据波谱数据和理化性质确定化学结构;MTT法测定化合物对人肝癌细胞系HepG2的体外抑制作用,用半数抑制浓度(IC50)评价其抗肿瘤活性。结果 共分离鉴定了11个化合物,分别为正二十四烷酸(Ⅰ)、正二十六烷酸(Ⅱ)、三油酸甘油酯(Ⅲ)、单棕榈酸甘油酯(Ⅳ)、3-甲氧基-4-羟基-反式苯丙烯酸正十六醇酯(阿魏酸正十六醇酯,Ⅴ)、丁香脂素(Ⅵ)、7-羟基-4′-甲氧基异黄酮(Ⅶ)、异甘草素(Ⅷ)、3-羟基-9-甲氧基紫檀烷(Ⅸ)、β-谷甾醇(Ⅹ)、胡萝卜苷(Ⅺ)。化合物Ⅸ对HepG2的IC50为10.69 μmol/L。结论 化合物Ⅰ～Ⅳ、Ⅵ为首次从该植物中得到;首次研究化合物Ⅸ的抗肿瘤活性,结果显示其对HepG2具有一定的细胞毒活性。     

蒙药小白蒿化学成分的研究

目的 研究小白蒿Artemisia frigida氯仿和醋酸乙酯提取物中的化学成分。方法 采用硅胶和LH-20柱色谱法进行分离,运用各种波谱法和标准品对照法鉴定分离得到的化合物。结果 鉴定了13个化合物,分别为槲皮素(Ⅰ)、木犀草素(Ⅱ)、5,7,3′-三羟基-4′-甲氧基黄酮(Ⅲ)、5,7,3′-三羟基6,4′-二甲氧基黄酮(Ⅳ)、5,3′-二羟基-6,7,4′-三甲氧基黄酮(Ⅴ)、5,3′-二羟基3,6,7,4′-四甲氧基黄酮(Ⅵ)、对甲基苯酚(Ⅶ)、7-羟基香豆素(Ⅷ)、7-甲氧基香豆素(Ⅸ)、咖啡酸(Ⅹ)、β-谷甾醇(Ⅺ)、6,7-二羟基香豆素(ⅩⅡ)、7-羟基-5,6-二甲氧基香豆素(ⅩⅢ)。结论 所有化合物均为首次从该植物中分得。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.