462例健康体检者甲状腺功能检测异常结果分析

目的回顾性分析462例健康体检者甲状腺功能检测结果,了解大连地区居民甲状腺疾病患病情况。方法收集2017-03-01—2018-03-31在某院健康管理中心进行甲状腺功能检测的健康体检者共462例,其中女322例,平均年龄(47.4±13.4)岁,男140例,平均年龄(53.8±13.8)岁,筛查出异常的结果进行综合分析。结果女性临床甲亢、亚临床甲亢、临床甲减、亚临床甲减的检出率分别为2.48%、3.42%、3.73%、9.32%。男性检出率分别为1.43%、1.43%、3.57%、7.86%。结论在健康体检人群中,20岁以上女性甲状腺疾病检出率较高,30岁以上男性的亚临床甲减检出率较高,而其临床症状不明显,只能通过甲状腺功能检测提示异常。     

上海市青浦区健康体检者甲状腺功能检测结果分析

目的分析上海市青浦区健康体检人群的甲状腺功能检测异常情况。方法选择2016-2018年来该院进行健康体检的青浦区城镇居民作为研究对象。所有研究对象清晨空腹抽取静脉血,使用雅培I2000全自动微粒子化学发光免疫分析仪检测血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)和促甲状腺激素(TSH)水平,对检测结果进行统计分析。结果在13 916例健康体检者中,检出甲状腺功能异常者788例,检出率为5.66%,其中甲状腺功能亢进(简称"甲亢")、亚临床甲亢、甲状腺功能减退(简称甲减)、亚临床甲减的检出率分别为0.34%、1.13%、0.19%、4.00%。亚临床甲状腺疾病的检出率(5.12%)高于临床甲状腺疾病的检出率(0.54%),差异有统计学意义(χ~2=531.604,P0.01)。2016、2017、2018年甲状腺功能异常检出率分别为5.63%、5.17%、5.88%,不同年份比较差异无统计学意义(χ~2=2.444,P0.05)。女性甲状腺功能异常检出率为6.58%,男性为4.02%,女性明显高于男性(χ~2=38.965,P0.01)。临床甲状腺疾病检出率男性和女性差异无统计学意义(χ~2=0.051,P0.05),亚临床甲状腺疾病检出率男性明显低于女性(χ~2=42.170,P0.01)。不同年龄组甲状腺功能异常的检出率高峰出现在≥70岁年龄组,检出率为10.37%。结论上海市青浦区甲状腺功能异常以甲亢和亚临床甲减为主,女性发病率高于男性。在健康体检中加入甲状腺功能检查是非常必要的。     

白癜风患者血清甲状腺自身抗体及甲状腺激素水平检测临床研究

目的通过检测白癜风患者血清甲状腺自身抗体阳性率及甲状腺激素水平,探讨白癜风与自身免疫性甲状腺疾病的关系。方法 334例白癜风患者设为白癜风组,279例健康体检人员为对照组,比较两组血清甲状腺自身抗体及甲状腺激素水平,并研究不同分期、分型的白癜风患者血清甲状腺自身抗体及甲状腺激素水平的差异性。结果白癜风组TG-Ab(20.06%)、TPO-Ab(21.57%)阳性率均高于对照组,差异均有统计学意义(P0.05);白癜风组FT3(5.21±1.64)、FT4(15.18±6.47)、TSH(1.73±0.69)平均水平均高于对照组,差异均有统计学意义(P0.05);不同分型白癜风患者自身抗体比较,均无统计学差异(P0.05);TSH节段型低于其他型别,差异均有统计学意义(P0.05),其他组任意两组比较均无统计学差异(P0.05);进展期、稳定期白癜风患者自身抗体、甲状腺激素水平比较均无统计学差异(P0.05)。结论白癜风与自身免疫性甲状腺疾病关系密切,对白癜风患者进行自身抗体及甲状腺激素水平的监测具有重要的临床意义。     

7473例健康体检者血脂和血糖检测结果分析

目的 分析不同性别和年龄健康体检者血脂指标与空腹血糖(FBG)水平以及异常情况分布.方法 选择曲靖市第二人民医院体检中心2019年1—9月接待的7473名健康体检者作为研究对象,根据年龄分为7组,分别为≤30岁、31～40岁、41～50岁、51～60岁、61～70岁、71～80岁、≥81岁组.采用酶法检测血脂指标〔包括...     

1254例健康体检者血脂和血糖检测结果分析

目的 了解体检人群血脂中总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、血糖(GLU)水平分布情况,为本地区心脑血管疾病、糖尿病的预防提供依据.方法 对1 254例健康体检者按年龄共分为6组(其中20~29岁组216例,30~39岁组264例,40~49岁组234例,50~59岁组 ...     

671例健康体检者血清两种抗原的检测结果分析

目的调查广东省健康体检者血清中EB病毒衣壳抗原(EB-VCA-IgA)和EB病毒早期抗原(EA-IgA)的阳性率及不同年龄间的差异。方法选取2008年6月11日至2009年6月11日送本中心体检的671例健康者血清,根据年龄分为8组,0～10岁组,11～20岁组,21～30岁组,31～40岁组,41～50岁组,51～60岁组,61～70岁组,71～80岁组。用酶联免疫吸附法分析EB-VCA-IgA和EA-IgA的阳性率。结果671例健康者中EB-VCA-IgA的阳性率为9.99%,EA-IgA的阳性率为4.77%.年龄与EB-VCA-IgA、EA-IgA的阳性率有关系,40岁以上的中老年人较高。结论对于鼻咽癌的高发区,健康体检套餐的防癌体检项目最好加EB-VCA-IgA和EA-IgA项目,以利于鼻咽癌的早期发现,早期诊断、早期治疗。     

体检人群自身抗体检测结果分析及意义

目的探讨体检人群自身抗体检测的临床意义。方法采用间接免疫荧光法(IIF)检测2 342名体检者血清标本中的抗核抗体(ANA)、抗平滑肌抗体(SMA)、抗壁细胞抗体(PCA)和抗线粒体抗体(AMA),采用线性免疫印迹法(LIA)检测ANA谱。男性与女性组抗体阳性率的比较采用χ2检验,不同年龄组抗体阳性率的比较采用秩和检验,趋势检验采用Gamma法。综合考虑自身抗体检测结果,IIF检测滴度≥1∶320且伴有其他自身免疫疾病相关实验室检测指标异常,将体检人群中可疑为自身免疫病者召回就诊。结果 2 342名体检者中,ANA、SMA、PCA和AMA的阳性率分别为24.77%、0.30%、2.65%、1.11%,1∶320的低滴度分别占79.69%、71.43%、51.61%、73.08%。ANA谱的阳性率为6.92%。ANA(χ~2=21.367,P0.05)、AMA(χ~2=4.528,P=0.033)、ANA谱(χ~2=17.647,P0.05)女性的阳性率高于男性,而SMA、PCA阳性率在男女性别间比较差异无统计学意义(P0.05)。ANA、PCA、AMA阳性率随年龄增加而升高,而SMA、ANA谱阳性率在不同年龄段间比较差异无统计学意义(P0.05)。召回26例就诊人群,确诊为自身免疫性疾病患者2例,原发性胆汁性肝硬化和结缔组织病各1例。结论体检人群中自身抗体有较高的阳性率,但是滴度较低,因此应重视体检人群自身抗体的检测结果,发挥自身抗体检测在疾病诊疗中的预警作用。     

健康体检者糖化血红蛋白检测结果分析

目的了解健康体检人群各年龄段糖化血红蛋白（HbA1c）测定的价值。方法采用高压液相离子交换色谱层析法测定1700例健康体检者HbA1c,A组：25--35岁296例,B组：36--45岁615例,C组：46--60岁452例,D组：60岁以上337例。结果①健康体检组HbA1c（5.66±0.93）%,其中A组（5.31±0.82）%,B组（5.46±0.73）%,C组（5.84±1.03）%,D组（6.09±1.00）%;②健康体检各年龄段组之间HbA1c测定结果A组与B组间差异无显著性意义（P〉0.05）,其他各组间比较差异均有显著性意义（P〈0.05）。结论中年以上人群的体检者进行HbA1c检测可以及早发现血糖异常,及时发现糖尿病。     

4895例健康体检者糖化血红蛋白检测结果分析

目的探讨糖化血红蛋白(HbA1c)在健康体检者中的检出情况。方法对4 895例健康体检者依据年龄分为A组(20~40)岁、B组(40~50)岁、C组(50~60)岁、D组(60~70)岁、E组(70岁)5组,用高效液相色谱法检测其HbA1c浓度。结果在4 895例HbA1c检测中,125例结果高于正常范围,其中A组6例,占0.29%;B组42例,占2.97%;C组32例,占3.95%;D组28例,占8.00%;E组17例,占6.80%。结论在健康体检人群中,随着年龄的增加,HbA1c检测结果偏高的比例随之增高。建议中老年人群定期检测HbA1c,以便及早发现血糖异常,及时发现糖尿病。     

High prevalence of thyroid hormone autoantibody and low rate of thyroid hormone detection interference

ObjectiveThyroid hormone autoantibody (THAb) is a common antibody in autoimmune disease and can interfere with the detection of thyroid hormone (TH). There was no research reporting the prevalence of THAb in Chinese and the rate of THAb interfering with TH detection.MethodsWe collected 114 patients with autoimmune thyroid disease (AITD) (Hashimoto''s thyroiditis, 57 cases; Graves’ disease, 57 cases), 106 patients with nonthyroid autoimmune diseases (NTAID), and 120 healthy subjects. According to the presence or absence of thyroid antibodies, patients with NTAID were divided into two groups: NTAID‐AITD and NTAID groups. Radioimmunoprecipitation technique was used to detect THAb in all subjects. TH was detected on Abbot and Roche platforms in patients with positive THAb.ResultsThe prevalence of THAb was 22.8% in Hashimoto''s thyroiditis and 45.6% in Graves’ disease. The prevalence of THAb in AITD group was lower than that in NTAID or NTAID‐AITD groups (34.2% vs. 61.5%, p = 0.014; 34.2% vs. 71.3%, p < 0.01). Among total 98 patients with positive THAb, TH levels of 9 patients were falsely elevated (9.18%).ConclusionThe prevalence of THAb in AITD patients was lower than that in NTAID patients. Although THAb had a high frequency in various autoimmune diseases, the prevalence of THAb interfering with TH detection was only 9.18%.     

Thyroid hormone concentrations and inhibition of thyroid stimulating hormone secretion in euthyroid subjects with autonomous thyroid nodules

Abstract. Sixty-four euthyroid patients with autonomous thyroid nodules and normal thyroxine (T₄) concentrations and tri-iodothyronine resin uptake have been studied. The serum tri-iodothyronine (T₃) concentration of the patients was 2.24 (±0.67) ng/ml, significantly higher than in a group of fifty-seven euthyroid control subjects (1.58 ± 0.30 ng/ml). When no extranodular tissue was visible on the basal thyroid scan, the T₃ was 2.31 (±0.63) ng/ml, significantly higher than in patients with some extranodular uptake on the basal scan (1.91 ± 0.42 ng/ml). There was no significant difference in the serum T₄ concentrations of the patients (7.37 ± 2.10 μg/100 ml) compared to the control group (6.88 ± 1.89 μg/100 ml). The T₄ concentrations were not correlated with total or partial inhibition of the extranodular tissue. The thyroid hormone concentrations were not directly correlated to the size of the nodule assessed by scan imaging. The thyroid stimulating hormone (TSH) concentration of the patients (1.52 ± 0.38 μU/ml) was significantly lower than in normals (2.49 ± 0.96 μU/ml). No significant difference was found in the TSH concentrations of patients with partial or total inhibition of extranodular tissue irrespective of the T₃ concentration. A thyrotrophin releasing hormone stimulation test in twelve patients did not increase the serum TSH, irrespectively of the T₃ concentration.
These data show the high frequency of elevated serum T₃ concentrations despite normal serum T₄ concentration in euthyroid patients with autonomous thyroid nodules. They confirm that inhibition of TSH secretion can occur when thyroid hormone concentrations are in the normal range.     

北京和上海地区部分健康人群血清甲状腺激素参考区间的初步调查

目的 建立北京和上海地区表面健康人群血清三碘甲状腺原氨酸(TT3)、甲状腺素(TT4)、游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺素(TSH)的参考区间.方法 按照美国国家临床生化学院检验医学应用准则中关于甲状腺疾病诊断与监测的实验室支持中的筛选要求,在北京、上海地区筛选体格检查和相关实验室检查均正常的表面健康人群390名(男221名,女169名),年龄18 ～ 65岁.采集受检者空腹血清,使用美国UniCel DXI 800全自动化学发光分析仪进行TT3、TT4、FT3、FT4、TSH的检测.不同性别、地区间上述各种甲状腺激素水平的比较采用两独立样本t检验,不同年龄组间的比较采用方差分析,两两比较采用LSD法.参考区间采用P2.5～P97.5表示.结果 对各种激素水平进行性别间比较,男性组TT3、TT4、FT3、FT4、TSH水平分别为(1.90±0.32) nmol/L、( 116.77±18.02) nmol/L、(5.28±0.67) pmoL/L、(11.54±1.97) pmol/L、(1.92±1.12) mIU/L,女性组分别为(1.82±0.32) nmo]/L、( 115.73±14.39) nmol/L、( 5.04±0.59) pmol/L、( 10.94±1.45) pmol/L、(2.37±1.86) mIU/L,TT3、FT3、FT4、TSH差异均有统计学意义(t分别为2.377、3.642、3.471、2.520,P均＜0.05).不同地区间结果比较,只有FT3在地区间差异有统计学意义,北京和上海分别为(5.01±0.63)、(5.41±0.61) pmol/L(t =6.410,P＜0.01),其他指标差异均无统计学意义.相关分析结果表明,TT4与年龄呈正相关(r=0.22,P＜0.01);TSH与年龄呈负相关(r=-0.12,P＜0.05).TT3、TT4、FT3、FT4、TSH参考区间分别为(1.22 ～2.50) nmol/L、(83.37～149.37) nmol/L、(3.88 ～6.48) pmoL/L、(7.70～14.86) pmol/L、(0.38 ～5.58) mIU/L.结论 北京和上海地区人群血清甲状腺激素水平与进口试剂盒给定的参考区间有差异,建立我国甲状腺激素参考区间具有重要意义.     

Pharmacokinetic-pharmacodynamic study of apomorphine's effect on growth hormone secretion in healthy subjects

Apomorphine (APO) stimulates growth hormone (GH) release via dopamine D2 receptors (DRD2). There is no specific study assessing the relationship between APO pharmacokinetic (PK) and the pharmacodynamic (PD) response e.g. GH release. The objective of the study is the PK-PD modelling of APO in healthy subjects. This is a randomized crossover study with s.c. administration of 5, 10, and 20 micro g/kg of APO in 18 healthy subjects. APO concentrations were modelled according to both a bi-compartmental model with zero-order absorption and a bi-compartmental model with first-order absorption. PK-PD relationship was modelled in accordance with the Emax Hill equation using plasma concentrations of APO calculated according to the bi-compartmental model with zero-order absorption. Modelled parameters were very similar to the experimental parameters. PK of APO was linear and there was no significant difference between the tested doses for AUC0--> infinity and Cmax (normalised to the dose 1 micro g/kg), t1/2alpha and t1/2beta. These parameters expressed as mean (CV%: SD/mean) were: 17.2 (26.9) ng/mL.min, 0.26 (33.3) ng/mL, 17.1 (54.2) and 45.2 (20.6) min, respectively (n = 53). An anticlockwise hysteresis loop (effect function of APO plasma concentration) appeared for each dose and each subject. The predicted and measured GH concentrations for all subjects and times were similar whatever the dose (P > 0.27). Emax values were 246 (121), 180 (107), 205 (139) ng/mL, respectively, and EC50 were 0.98 (48.1), 1.70 (62.3), 3.67 (65.2) ng/mL, respectively at dose 5, 10, and 20 micro g/kg (P < 10-4). APO and GH concentrations were predicted with good accuracy using bi-compartmental with zero-order absorption PK model and sigmoid Emax PD model, respectively.     

北京市体检人群EB病毒联合抗体检测结果分析

目的通过分析3项EB病毒(EBV)抗体联合检测结果,了解体检人群EB病毒感染情况,为鼻咽癌早期筛查提供技术支持。方法选取2017年1月1日—2018年6月30日在北京航天总医院健康管理中心进行EBV(Rta)-IgG、衣壳抗原(VCA)-IgA、早期抗原(EA)-IgA 3种抗体联合检测的体检人员共4251名,采用SPSS 16.0软件进行统计分析。结果4251名受检者中3种EBV抗体单项阳性率的比较差异有统计学意义(χ2=12.465,P=0.000),阳性率由高至低的顺序依次为VCA-IgA、EA-IgA、Rta-IgG;不同性别间VCA-IgA阳性率男性高于女性(P=0.000);不同年龄组3种EBV抗体阳性率总体随年龄增长而增高,EA-IgA(P=0.000)及Rta-IgG(P=0.0038)在不同年龄组中阳性率有显著差异,在61岁以上的受检者中EA-IgA和Rta-IgG阳性率最高,分别为6.3%及3.7%,VCA-IgA在51岁以上的受检者中阳性率最高;92.9%的受检者3项检测均为阴性,1项阳性者占6.4%,2项阳性者占0.7%,未发现3项阳性者;抗体阳性检测结果男性与女性聚集情况比较无差别,抗体检出阳性受检者在耳鼻喉科检查发现鼻炎、鼻窦炎及其他异常比例较高。结论3种EBV抗体的阳性率与性别和年龄都有一定的关系,50岁以上的人群应作为重点筛查对象。EBV抗体联合检测阳性受检者推荐鼻咽镜检查,为鼻咽癌的早期筛查提供依据。     

114例慢性特发性荨麻疹患者血清甲状腺自身抗体和激素检测的意义

目的通过检测慢性特发性尊麻疹（CIU）患者血清抗甲状腺过氧化酶抗体（TPOAb）、抗甲状腺球蛋白抗体（TGAb）、促甲状腺激素受体抗体（TRAb）这三种主要的甲状腺自身抗体和游离三碘甲状球原氨酸（FT3）、游离甲状腺素（FT4）以及促甲状腺激素（TSH）水平,探讨其与慢性特发性荨麻疹发病的相关性。方法对114例慢性特发性荨麻疹患者和59倒健康对照采用电化学发光发检澍血清甲状腺自身抗体和激素。结果慢性特发性荨麻疹患者血清TPOAb、TGAb、TRAb阳性率分剐为28．2％、18．8％、9．7％皆高于健康对照（P〈0．05）,有统计学意义;FT3、FT4和TSH与对熙组比较无统计学差异（P〉0．05）。结论甲状腺自身抗体畀常是慢性特发性荨麻疹发病因素之一,对原因不明的慢性特发性荨麻疹患者进行血清甲状腺自身抗体检测有助于病因明确,指导治疗。     

Renal excretion of antidiuretic hormone in healthy subjects and patients with renal failure

Urinary clearance of antidiuretic hormone (ADH) has been measured under basal conditions and during intravenous administration of arginine vasopressin in ten healthy subjects, and only under basal conditions in 18 patients with chronic renal failure and seven patients with acute renal failure at the polyuric phase of the disease. In healthy subjects studied under conditions of mild water diuresis plasma concentration, urinary excretion rate, urinary clearance and fractional clearance of ADH were 3.3 +/- 0.36 pg/ml, 25.2 +/- 5.5 pg/min, 7.5 +/- 1.2 ml/min and 6.4 +/- 1.0% (means +/- SEM) respectively. When plasma ADH was raised to levels between 7 and 26 pg/ml during intravenous administration of the hormone, urinary excretion rate and urinary clearance of ADH increased. Tubular reabsorption of ADH did not reach a plateau but progressively increased in the range of plasma ADH studied. In patients with chronic renal failure, plasma concentration, urinary excretion rate, urinary clearance and fractional clearance of ADH were 2.8 +/- 0.19 pg/ml, 9.4 +/- 2.0 pg/min, 3.4 +/- 0.6 ml/min and 10.0 +/- 2.9% (means +/- SEM) respectively. Urinary excretion rate and urinary clearance were significantly lower than in healthy subjects. In patients with acute renal failure, plasma concentration, urinary excretion rate, urinary clearance and fractional clearance of ADH were 4.6 +/- 0.47 pg/ml, 52.8 +/- 15.8 pg/min, 9.5 +/- 2.7 ml/min and 24.9 +/- 4.4% (means +/- SEM) respectively. Urinary excretion rate and fractional clearance were higher than in healthy subjects and patients with chronic renal failure.(ABSTRACT TRUNCATED AT 250 WORDS)     

Cyanocobalamin absorption in the elderly: results for healthy subjects and for subjects with low serum cobalamin concentration

We examined the intestinal absorption of cyano[57Co]cobalamin from a non-protein-bound test dose given to 38 subjects from a population of elderly. The subjects were 76 years old and were apparently free from conditions known to affect cyanocobalamin absorption. Their gastric mucosal function was normal, as judged from determinations of serum gastrin and pepsinogen I. The urinary excretion of radioactivity during the first 24 h was 24(SD 7)%, range 8.6 to 45.2%, corresponding to a health-associated reference interval of 10 to 38%. The results indicate that cyanocobalamin absorption does not decline during normal aging. Duplicate studies were performed in another 20 subjects (70-81 years old) from the same population study; these subjects had a serum cobalamin concentration less than 130 pmol/L. The imprecision (CV) was 23%.     

采用健康体检人群甲状腺激素数据估计和验证参考区间

摘要:目的验证实验室 估计的甲状腺激素参考区间与现行使用的试剂说明书推荐参考区间和WS/T 404.10--2022 推荐参考区间的一致性,选择适合该院健康人群甲状腺激素的参考区间。方法使用 间接取样技术,通过回顾性分析2022年12月至2023年8月期间,苏州市独墅湖医院体检中心健康体检者甲状腺激素检测结果,采用非参数法估计不同性别及年龄的甲状腺激素P2s~Pogns参考区间和95%置信区间,探讨不同性别、年龄组的数据分布及差异的显著性,与行业标准( WS/T 404. 10- -2022)及罗氏试剂说明书推荐的参考区间进行差异比较。结果TSH、FT3、FT4、TT3性别分组差异具有统计学意义(P<0.001,Z>Z* ),TT4性别分组差异无统计学意义(P=0.998,Z相似文献