可乐丽菲露SE BOND用于直接盖髓术的组织学评价

目的:对可乐丽菲露SEBOND在人体用于直接盖髓术进行组织学评价。方法:选择24名志愿者共45颗健康人第三磨牙,41颗在牙合面制备Ⅰ类洞并在洞底作人工穿髓孔,然后分为2组,实验组以可乐丽菲露SEBOND盖髓,对照组以氢氧化钙盖髓。4颗作为空白对照。所有实验牙齿分别在盖髓后的7、30、90d后拔除并进行组织学检测。结果:实验组和对照组志愿者均未出现术后实验牙齿敏感症状。病理检测显示,在7d和30d的观察期内2组均有轻度到中度的炎性反应,但可乐丽菲露SEBOND组的炎性反应较氢氧化钙组轻,其组织学评价分级上的差别有统计学意义(P<0.05)。在90d的观察期内2组均无明显的炎性反应,而牙本质桥的形成在氢氧化钙组显著多于可乐丽菲露SEBOND组,氢氧化钙组7例标本中有6例标本有牙本质桥的形成,可乐丽菲露SEBOND组7例标本中有3例形成,其差别有统计学意义(P<0.05)。结论:可乐丽菲露SEBOND对牙髓的刺激性较小,但诱导牙本质形成的能力较氢氧化钙弱。     

可乐丽菲露SEBOND应用于Beagles犬直接盖髓术的组织学评价

目的　将可乐丽菲露SE BOND用于beagles犬直接盖髓术后,进行组织学分级评价。方法　选择6只bea- gles犬共130颗健康牙齿,在颊面制备Ⅴ类洞并在洞底作人工穿髓孔,然后分为2组,实验组以可乐丽菲露SE BOND 盖髓,对照组以氢氧化钙盖髓。所有实验牙齿分别在盖髓后的第7、30、90天拔除,并进行组织学评价。结果　病理 学评价显示,在7 d的观察期内2组的牙髓均呈轻度到中度的炎性反应,可乐丽菲露SE BOND组的炎性反应较氢氧 化钙组轻,但其差别没有统计学意义。在30 d和90 d的观察期内2组均无明显的炎性反应,而牙本质桥的形成在 氢氧化钙组显著多于可乐丽菲露SE BOND组,其差别有统计学意义(P<0·05)。结论　可乐丽菲露SE BOND对牙 髓的刺激性较小,但诱导牙本质形成的能力较氢氧化钙弱。     

可乐丽菲露SEBOND治疗牙本质过敏症的疗效观察

目的 对比观察可乐丽菲露SEBOND自酸蚀黏结剂和75%氟化钠甘油糊剂治疗牙本质过敏症的临床疗效.方法 2007年7月至2008年5月在大连大学附属中山医院口腔科就诊的牙合面牙本质过敏症患者112例,共196颗患牙,随机分为治疗组56例103颗患牙,用可乐丽菲露SEBOND自酸蚀黏结剂脱敏治疗;对照组56例93颗患牙,用75%氟化钠甘油糊剂脱敏治疗,在统一标准下进行即刻和3个月后的疗效对照分析.结果 两组之间即刻疗效差异无统计学意义,3个月后的疗效差异具有统计学意义,可乐丽菲露SEBOND自酸蚀黏结剂脱敏效果明显优于氟化钠甘油糊剂.结论 可乐丽菲露SEBOND自酸蚀黏结荆对牙本质过敏症具有较好的,临床治疗效果.     

可乐丽菲露SE BOND防治固定义齿基牙牙本质过敏的疗效观察

目的 探讨评价可乐丽菲露SE BOND对活髓基牙预备后牙本质过敏症的防治效果。方法 将118例(共238颗基牙)行冠桥固定修复的患者随机分为可乐丽菲露SE BOND组、Gluma脱敏剂组(对照组)、75%NaF组(对照组)3组,所有基牙采用暂时冠修复、氧化锌丁香油酚粘固剂粘固。7～10d后试戴修复体,观察治疗效果,并进行统计学分析。结果 经3种药物处理过的基牙过敏症状均有明显改善,可乐丽菲露SE BOND组(有效率95.18%)的疗效优于75%NaF组(有效率56.58%,P<0.05),而可乐丽菲露SE BOND组和Gluma脱敏剂组(有效率92.41%)间则无统计学差异(P>0.05)。结论 可乐丽菲露SE BOND可以有效防治全冠活髓基牙预备后牙本质过敏症的发生。     

可乐丽菲露修复楔状缺损的疗效观察

楔状缺损是常见的牙体硬组织疾病,中老年人的发病率高达90%以上[1],常伴有不同程度的牙本质过敏症。本科对楔状缺损牙用可乐丽菲露修复,取得了较好疗效,现介绍如下。     

玻璃离子、复合体、复合树脂对人牙髓细胞的细胞毒性试验研究

目的 :研究玻璃离子、复合体、复合树脂牙色材料对体外培养的人牙髓细胞的细胞毒性作用。方法 :采用MTT法测定上述 3种牙色材料的浸渍液对体外培养的人牙髓细胞的细胞毒性。结果 :与对照组相比 ,7d的牙色材料浸渍培养液对体外培养的人牙髓细胞有抑制作用 ,其中复合树脂的抑制人牙髓细胞的增殖有显著性 (P <0 .0 5 ) ;但 3d的浸渍培养液却呈现出不同的细胞毒性作用 :玻璃离子仍有一定的抑制细胞增殖作用 ,复合体基本无作用 ,而复合树脂却有显著的促进细胞增殖作用 (P <0 .0 5 )。结论 :牙色材料的细胞毒性作用与其牙色材料的种类相关。     

复合树指单体对人牙髓细胞毒性的研究

目的：评价复合树脂单体对人牙髓的毒性作用,探讨不同细胞系对检测敏感性的影响。方法：选用人牙髓细胞（ＬＳＣ）为实验细胞,以Ｌ－９２９小鼠成纤维细胞作为对照细胞系,采用ＭＴＴ比色分析法,对两种牙科用复合树脂单体（ＴＥＧＤＭＡ和ＵＤＭＡ）进行体外细胞毒性研究。结果：在低于ＩＣ５０的各浓度组中,ＬＳＣ细胞的生存率高于Ｌ－９２９细胞;ＭＴＴ比色法作为一种能定量检测牙科材料细胞毒性的有效方法,实验时应注重考虑     

复合树脂细胞毒性检测方法的进展

良好的生物相容性是保证复合树脂临床应用安全有效的重要标准,体外细胞毒性实验作为生物学评价筛选实验最常用的项目之一,对复合树脂临床应用具有指导意义。该文就体外细胞毒性实验方法的进展作一综述。     

牙科复合树脂及其浸出成分的细胞毒性

目的：观察4种含有不同成分的牙科复合树脂的细胞毒性,通过高效液相色谱法分析其浸出的成分。材料和方法：将复合树脂（Z100,Solitaire 2,Filtek P60．Synergy）制备成圆盘状测试试样,采用Balb／C 3T3成纤维细胞进行细胞毒性实验。在37℃条件下．实验第一部分为细胞与树脂接触72h（直接法）,第二部分则是细胞与树脂的乙醇浸泡提取液接触24h（间接法）。用MTT法{[3（4,5二甲基噻唑2）2,5二苯基]四氮唑嗅盐法}检测细胞存活率。结果：牙科复合树脂及其乙醇浸泡提取液对Balb／C 3T3成纤维细胞有毒性作用。单因素方差分析显示直接接触和间接接触各组间的差异均有统计学意义（P〈0.001）。组间比较（Tukey检验．α=0.05）显示直接法检测Synergy的毒性显著低于其他树脂．Solitaire 2毒性显著高于其他树脂。但是．间接法检测则为Synergy的浸泡提取液毒性显著高于其他树脂的提取液。采用高效液相色谱法检测各树脂中Bis—GMA,TEGDMA．UDMA．bis-EMA以及双酚A的含量,结果显示上述成分存在与否和其浓度各不相同。结论：牙科树脂的细胞毒性水平与其化学成分、浸泡介质以及浸出的成分和量相关。     

Histological evaluation of direct pulp capping with a self-etching adhesive and calcium hydroxide on human pulp tissue

Aim  To evaluate human pulp tissue response following direct pulp capping with a self-etching adhesive: Clearfil SE BOND (SB).
Methodology  Forty-five sound teeth from 20 subjects were used. Forty-one teeth had their pulp mechanically exposed at the base of a Class 1 cavity preparation and were divided into two groups: group 1, teeth were capped with SB ( n  = 21), and group 2, with calcium hydroxide cement (CH) ( n  = 20). Four teeth were maintained intact as an untreated control group. After 7, 30 and 90 days, respectively, 15 teeth were extracted and processed for light microscopic examination. Pulp healing and bacterial microleakage were assessed by haematoxylin and eosin, Masson trichrome and Brown and Brenn stain techniques. The data were analysed statistically by using the Mann–Whitney U test.
Results  After the 7-day observation period, the inflammatory reaction in the SB group was slight and significantly less severe than that of the CH group ( P  < 0.05). After the 30- and 90-day observation periods, the inflammatory reaction was slight in both groups, but specimens with dentine bridge formation in the SB group were significantly less common than those in the CH group ( P  < 0.05).
Conclusions  Clearfil SB had good biocompatibility with human pulp tissue, but its ability to induce reparative dentine was significantly lower than that of calcium hydroxide.     

Cytotoxicity of flowable resin composite on cultured human periodontal ligament cells compared with mineral trioxide aggregate

Aim: To investigate the cytotoxicity of three flowable resin composites that potentially useful as retrograde filling materials, compared with mineral trioxide aggregate (MTA). Methods: Ten standard cylinder discs were used for each of the tested materials: Tetric Flow, Filtex Flow, Aeliteflo, and MTA, which were prepared under aseptic conditions. Cytotoxicity of eluates from all materials after 1–4 days’ immersion in culture medium and direct contact cytotoxicity were evaluated using cultured human periodontal ligament cells (PDLC). The colorimetric (3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide) assay and scanning electron microscope of cell morphology (direct contact only) were used. Results: No eluates of set materials demonstrated cytotoxicity at any concentration or elution time. Freshly‐mixed MTA was cytotoxic in direct contact, but not set MTA. Freshly‐mixed Aeliteflo was also cytotoxic, as was set material up to 2 days’ elution. With morphological assessment, some differences were seen among resin composites, but all changes were rated as slight using the International Standard Organization criteria. Conclusions: Of all of the materials tested, Tetric Flow showed the least cytotoxic effects on PDLC. Further research is needed to determine the clinical usefulness of flowable composites as retrograde filling materials.     

Cytotoxicity of a silorane-based dental composite on human gingival fibroblasts

AIM: To evaluate the direct and indirect biocompatibility of Filtek Silorane on human gingival fibroblastic cells. METHODS: Sixty-three standardized cylindrical specimens (8 mm diameter and 2 mm thickness) of restorative material were prepared using a light emitting diode-curing unit. The sample were built up in one increment and divided in 2 groups. In the first group, 21 samples (unpolished samples) were left without a specific polishing procedure; in the second one, 42 samples (polished samples) were polished with 4 different grains of discs. Fibroblast cultures, obtained from gingiva of 2 subjects without systemic and oral disease, were used to assess the direct and indirect biocompatibility. Cells cultured for 48 h in normal culture medium were used as a control. RESULTS: The scanning electron microscope observations of fibroblasts cultured on the silorane samples, either polished or unpolished, confirmed the good biocompatibility of the material, favouring the cellular spreading. 3-dimethylthiazol-2, 5-diphenyltetrazolium bromide tests showed a significant reduction (P < 0.01) of gingival fibroblasts viability cultured both in polished samples (90.05% ± 19.00%) and unpolished samples (78.15% ± 11.00%) compared with the control. Cells growth in medium conditioned with the samples for 1 wk showed a significant viability reduction (P < 0.01) compared to the control. A reduction of cell viability was observed even in the groups containing the material for 3 wk (polished: 89.45% ± 10.00%; unpolished: 65.97% ± 10.00%), even if the cytotoxicity was reduced after this long time exposure. CONCLUSION: Although the poor chromatic availability of this material remains a big limit that restricts its use to posterior sectors, the silorane-based material can be considered an option to perform restorations when aesthetic demands are not the priority, such as the class II restorations     

Cytotoxicity of polymerized resin cements on human dental pulp cells in vitro

ObjectivesThis study was designed to evaluate the cytotoxicity of several resin-based cements (Panavia F, Super-Bond C&B, Chemiace II) after polymerization on cultured human dental pulp cells.MethodsAfter polymerization, specimens from three resin-based cements were eluted with fresh Dulbecco's modified Eagle's medium (DMEM) without serum for 72 h, at 37 °C, using 0.4 g of each substance per milliliter of fresh medium. Elutes obtained during this step were passed through a 0.22-μm filter and diluted with the culture medium by a ratio of 75%, 50%, 25% (v/v). Cytotoxicity of elutes were evaluated by the relative growth rates (RGR) of pulp cells with a modified 3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. The RGR of pulp cells were statistic analyzed by the one-way analysis of variance among the groups.ResultsThe RGR of cells exposed to 100% concentration of elution of Panavia F, Super-Bond C&B, and Chemiace II were 74.42%, 85.54%, and 82.39%, respectively. The RGR increased along with the elution of cements diluted. There was significant difference between the Panavia F group and Super-Bond C&B group (p < 0.01), but there was no significant difference in the cytotoxicity between Chemiace II and Super-Bond C&B.ConclusionsAfter polymerization, three resin-based cements (Panavia F, Super-Bond C&B, Chemiace II) induced slight cytotoxicity. The sensitivity of cytotoxicity to human pulp cells depended on the resin-based cements and the concentration of the elution. Super-Bond C&B is the least cytotoxic agent among the three resin-based cements.     

牙本质粘结剂对人牙髓细胞毒性的体外研究

目的：评价全酸蚀牙本质粘结剂和自酸蚀牙本质粘结剂对人牙髓细胞的毒性作用.方法：用人牙髓细胞为实验细胞,采用MTT比色分析法,对4 种牙本质粘结剂（Prime ＆ Bond NT,Single Bond,Xeno Ⅲ,iBond）进行体外细胞毒性研究.结果：不同浓度的粘结剂稀释液均可使人牙髓细胞的形态有所改变.4 种牙本质粘结剂的细胞毒性有显著性差异,且作用时间和浓度的改变对其细胞毒性有影响.全酸蚀粘结剂比自酸蚀粘结剂的细胞毒性强.结论：4 种牙本质粘结剂在体外对人牙髓细胞均有一定程度的细胞毒性,其中Single Bond的毒性较强,临床使用粘结剂时应合理选择粘结剂和掌握固化时间.     

Grandio纳米树脂与可乐丽树脂充填治疗后牙深龋的疗效比较

目的 对比Grandio纳米树脂与可乐丽树脂充填治疗后牙深龋的临床效果.方法 选择后牙深龋患者276例398颗,随机分为2组.试验组138例197颗患牙使用Grandio纳米树脂充填,对照组138例201颗患牙使用可乐丽树脂充填.术后随访2年,对比两组充填体解剖形态、固位、边缘密合度及牙齿继发龋、牙髓、根尖周及牙周状况.结果 治疗后6个月试验组和对照组成功率分别为97.46％和97.01％,成功率差异无统计学意义(x2=0.074,P=0.786).治疗后2年试验组和对照组成功率分别为95.43％和88.06％,差异有统计学意义(x2 =7.110,P=0.008),其中Ⅰ类洞成功率差异无统计学意义,Ⅱ类洞成功率试验组高于对照组,差异有统计学意义(x2=8.728,P=0.003).结论 Grandio纳米树脂充填治疗后牙深龋Ⅱ类洞远期疗效较佳.     

Cytotoxicity evaluation of dental resin composites and their flowable derivatives

The release of components from dental composite into surrounding tissue may cause an adverse tissue reaction. Thus, this study investigated the cytotoxicity of three types of dental composites with their flowable derivatives and determined the compounds released from these materials by high-performance liquid chromatography (HPLC) analysis. Fifteen specimens from each composite (Admira, Z250, Tetric Ceram) with fifteen of their flowables (Admira Flow, Tetric Flow, Feltik Flow) were prepared in the form of discs and divided into two groups of 10 and 5 for each material. The first group (10 discs) was used to evaluate the cytotoxicity of the material on balb/c 3T3 fibroblasts by measuring cellular metabolic activity (3{4,5-dimethylthiazol-2-yl}-2,5-diphenyltetrazolium bromide [MTT] assay) relative to Teflon controls, while the second group (5 discs) was used to determine the leached components from each material into culture medium by HPLC analysis. The results revealed that Z250 and Tetric Ceram were less cytotoxic than their flowable derivatives. However, the ormocer, Admira, was significantly more cytotoxic than Admira Flow. Among the standard composites, Tetric Ceram was the least cytotoxic and Admira the most. Furthermore, Tetric flow was the most cytotoxic and Admira flow was significantly the least cytotoxic among the flowable materials tested. HPLC analysis revealed bisphenol A glycerolate dimethacrylate (bis-GMA) and triethylene glycol dimethacrylate (TEGDMA) in the eluates of all the materials, while urethane dimethacrylate (UDMA) was present in all eluates except that of Feltik Flow. In conclusion, the flowable derivatives are more cytotoxic than the traditional composites whereas the ormocer Admira Flow is less cytotoxic than the Admira composite.     

4种牙体修复材料对人牙髓细胞毒性的实验研究

目的：比较4种牙体修复材料--Dyract AP、Ceram X、Quixfil U及Surefil复合树脂对人牙髓细胞的生物学作用.方法：制备4种牙体修复材料的细胞培养基（DMEM）浸出液,采用四唑盐MTT比色分析法测定材料对人牙髓细胞生长的影响;通过扫描电镜观察人牙髓细胞在不同材料表面的生长情况.结果：人牙髓细胞在4种复合树脂浸出液作用下,细胞相对增值率（RGR）与对照组相比均有显著性差异（P＜0.05）,其中Surefil的RGR与其他3种材料相比有显著性差异（P＜0.05）,而Ceram X、Quixfil U、Dyract AP 3种材料之间RGR无显著性差异（P＞0.05）;扫描电镜观察到人牙髓细胞在4种材料表面均可贴附,且生长良好.结论：4种牙体修复材料均对牙髓细胞的生长无不良影响,具有良好的生物相容性.