冠心病患者CCL20-CCR6轴及IL-17表达与预后的相关性研究

目的 探讨冠心病患者趋化因子配体20(CCL20)、趋化因子受体6(CCR6)和白介素-17(IL-17)的表达与预后的关系。方法 选择2016年7月至11月温州医科大学附属第一医院心内科收治的急性心肌梗死（AMI）患者96例,稳定型心绞痛（SAP）患者45例,无冠心病患者65例。采用酶联免疫吸附法检测血浆CCL20和IL-17水平。实时定量PCR检测单核细胞中CCR6 m RNA水平。采用logistic回归分析确定冠心病的危险因素。Gensini评分评价冠状动脉狭窄程度,分析CCL20、CCR6和IL-17与Gensini评分的相关性。对AMI患者进行随访以观察主要不良心血管事件（MACE）风险。结果AMI组和SAP组患者血浆CCL20和IL-17水平、外周血单核细胞CCR6 m RNA表达水平均显著高于对照组（均P<0.01）。多因素分析显示,CCL20和IL-17是冠心病发生的独立危险因素（均P<0.01）。CCR6 m RNA、CCL20和IL-17水平均与Gensini评分呈正相关（r=0.32、0.65、0.56,均P<0.01）。随访期间CCL20表达...     

趋化因子CCL20及其受体CCR6在实验性结肠炎小鼠中的表达及其意义

背景：溃疡性结肠炎（UC）的病因和发病机制尚不完全明确，趋化因子异常可能在其发生、发展中起重要作用。目的：研究趋化因子CCL20及其受体CCR6在葡聚糖硫酸钠（DSS）诱导的实验性结肠炎小鼠结肠黏膜中的表达以及CCL20与CCR6的相关性，探讨两者在结肠炎发病机制中的作用。方法：30只雌性BALB／c小鼠分为对照组和模型组。模型组小鼠自由饮用5％DSS溶液7d，建立实验性结肠炎模型；对照组小鼠自由饮用蒸馏水7d。第8d处死小鼠，观察疾病活动指数（DAI）、结肠组织学评分。以逆转录聚合酶链反应（RT．PCR）检测小鼠结肠黏膜CCL20、CCR6mRNA表达，以免疫组化和蛋白质印迹法检测结肠黏膜CCL20、CCR6蛋白表达，并分析CCL20与CCR6的相关性。结果：模型组DAI和结肠组织学评分均显著高于对照组（P〈0．01）；CCL20、CCR6mRNA和蛋白表达显著高于对照组（P〈0．01），且与结肠炎症程度呈正相关。CCL20mRNA表达与CCR6mRNA表达有明显相关性（r＝0．763．P=0．000071）。结论：CCL20和CCR6表达参与了结肠炎的发生和发展，两者相互作用可能在结肠炎局部结肠组织破坏和病理变化中起重要作用，有望成为UC的潜在治疗靶点。     

趋化因子CCL20及其受体CCR6在急性坏死性胰腺炎中的表达及意义

目的 探讨CC趋化因子配体20(CCL20)和CC趋化因子受体6(CCR6)在实验性急性坏死性胰腺炎(ANP)发病机制中的作用.方法 48只SD大鼠按完全随机法分为ANP组和对照组.采用胆胰管逆行注射4%牛磺胆酸钠建立大鼠ANP模型,以注射等容积生理盐水作为对照组.术后1、3、6、12 h分批处死大鼠,取血检测血清淀粉酶,胰腺组织常规病理检查并评分,免疫组化和RT-PCR检测胰腺组织中CCL20、CCR6 mRNA及蛋白表达.结果 ANP大鼠血清淀粉酶和胰腺组织病理评分明显高于对照组.ANP组术后胰腺组织CCL20 mRNA及蛋白表达呈时间依赖性增强(P＜0.05);术后6 h胰腺组织中CCR6 mRNA明显高于对照组(0.88±0.05对0.23±0.09,P＜0.01),术后12 h CCR6mRNA表达较6 h时降低,但仍高于对照组(0.37±0.10对0.15±0.07,P＜0.05),CCR6蛋白变化与其mRNA变化一致.结论CCL20和CCR6参与ANP的发生和发展.     

趋化因子及其受体在消化道肿瘤生物学行为中的作用

对趋化因子的最初认识是白细胞趋化性迁移作用,近年来,众多的研究表明,趋化因子及其受体对肿瘤细胞生长、血管生成、浸润和转移等过程有显著影响,这为探讨肿瘤的形成机制及抗肿瘤治疗提供了新的视角.此文就近年来有关趋化因子及其受体在消化道肿瘤生物学行为中作用的研究进展作一综述.     

血清趋化因子CCL18、CCL21水平与慢阻肺合并肺癌的相关性

目的 分析慢性阻塞性肺疾病(慢阻肺)合并肺癌患者血清CC家族趋化因子配体(CCL)18(CCL18)及CCL21与慢阻肺患者合并肺癌的关系.方法 回顾性分析,收集2018年3月-2019年11月我院91例慢阻肺合并肺癌患者资料为合并组,收集同期97例慢阻肺患者资料为对照组,检测两组CCL18、CCL21水平;分析各血清...     

趋化因子CXCL16/CXCR6轴与乳腺癌相关关系的研究进展

乳腺癌是中老年女性发病率及病死率高居首位的恶性肿瘤,且呈年轻化趋势。乳腺癌发生、发展过程受诸多因素调控,其病因及发病机制尚未完全清楚。该文旨在对趋化因子CXCL16/CXCR6轴的结构、功能及其在乳腺癌发生、发展、侵袭过程中调控作用进行综述,为乳腺癌的分子生物学研究及靶向抗肿瘤治疗提供新思路。     

CCL20单克隆抗体对小鼠嗯唑酮结肠炎的治疗作用

背景：趋化因子是一类具有趋化作用的小分子细胞因子,与免疫和炎症反应密切相关。炎症性肠病（IBD）是一组肠道慢性炎症性疾病．免疫反应异常为其特征性表现之一。目的：研究趋化因子CCL20及其受体CCR6在小鼠实验性结肠炎中的表达以及抗CCL20治疗对实验性结肠炎的疗效。方法：30只昆明小鼠随机分为正常对照组、结肠炎模型组和CCL20单抗组．后两组以嗯唑酮溶液灌肠诱导结肠炎模型。造模后每天予单抗组小鼠CCL20单抗1mg／kg腹腔注射一次,连续4d。第5d行疾病活动指数（DAI）评估后处死小鼠,行结肠大体形态和组织学损伤评分,测定结肠组织髓过氧化物酶（MPO）活性;以荧光定量聚合酶链反应（PCR）和酶联免疫吸附测定（ELISA）检测结肠组织CCL20mRNA和蛋白表达：分离肠黏膜固有层单个核细胞（LPMC）,流式细胞仪分析CCR6阳性CD4^＋T细胞比例。结果：与正常对照组相比．结肠炎模型组的DAI、结肠大体形态和组织学损伤评分、MPO活性以及结肠组织CCL20mRNA和蛋白表达、LPMC中CCR6阳性CD4^＋T细胞比例均显著增高（P〈0．05）;而CCL20单抗组上述指标均较结肠炎模型组显著降低（P〈0．05）。结论：CCL20／CCR6在嗯唑酮诱导的小鼠实验性结肠炎中表达增高,以CCL20单抗阻断CCL20／CCR6可有效缓解结肠炎症。     

趋化因子受体CCR7在胃癌组织和细胞株中过度表达

背景:趋化因子受体CCR7高表达与肿瘤淋巴结转移等因素密切相关。目的:检测CCR7在胃癌组织和细胞株中的表达情况,探讨CCR7在胃癌中的作用。方法:采用实时PCR技术检测30例胃癌组织及其配对癌旁组织中的CCR7 mRNA表达,RT-PCR检测人胃癌细胞株SGC-7901和AGS中的CCR7 mRNA表达。分析胃癌组织中的CCR7mRNA表达与胃癌临床病理特征间的相关性。结果:CCR7 mRNA在胃癌组织中的表达水平显著高于其配对癌旁组织(P0.05),人胃癌细胞株SGC-7901和AGS中均存在明显CCR7 mRNA表达。CCR7 mRNA表达与胃癌浸润深度、淋巴结转移和TNM分期相关(P0.05)。结论:CCR7在胃癌中存在过度表达,可能参与了胃癌的进展过程。     

CCR6-CCL20对食管鳞癌患者体内Th17细胞分布的影响

目的研究CCR6-CCL20对食管鳞癌患者体内Th17细胞分布的影响。方法采用流式细胞术检测食管鳞癌患者手术切除的癌组织、癌旁组织及外周血中Th17细胞的比例及CCR6+Th17细胞的比例。RT-PCR方法检测组织中CCL20 mRNA水平。结果与癌旁组织及外周血相比,癌组织中Th17细胞明显升高(P<0.01);癌组织中CCR6+Th17细胞的比例高于癌旁组织及外周血(P<0.01);癌组织中CCL20 mRNA水平高于癌旁组织(P<0.01),并与Th17呈正相关。结论 CCR6-CCL20趋化轴可能在食管鳞癌患者Th17细胞浸润到肿瘤组织中发挥重要作用。     

chemokine/chemokine receptor pair ccL20/ccR6 in human colorectal malignancy:An overview

Chemokines belong to a superfamily of small, cytokinelike proteins, which induce multiple physiological functions, particularly cytoskeletal rearrangement and compartment-specific migration through their interaction with G-protein-coupled receptors. Chemokines and their receptors have been widely acknowledged as essential and selective mediators in leukocyte migration in inflammatory response. It is now established that the chemokine/chemokine receptor system is also used by cancer cells to direct lymphatic and haematogenous spreading and additionally has an impact on the site of metastatic growth of different tumours. In recent years an increasing number of studies have drawn attention to CC-chemokine cysteine motif chemokine ligand 20(CCL20) and its physiological sole receptor CCR6 to play a role in the onset, development and metastatic spread of various gastrointestinal cancer entities. Among various cancer types CCR6 was also demonstrated to be significantly overexpressed in colorectal cancer(CRC) and stimulation by its physiological ligand CCL20 has been reported to promote CRC cell proliferation and migration in vitro. Further, the CCL20/CCR6 system apparently plays a role in the organ-selective liver metastasis of CRC. Here we review the literature on expression patterns of CCL20 and CCR6 and their physiological interactions as well as the currently presumed role of CCL20 and CCR6 in the formation of CRC and the development of liver metastasis, providing a potential basis for novel treatment strategies.     

经内镜切除消化道黏膜下肿瘤

目的 探讨内镜切除消化道黏膜下肿瘤(SMT)的疗效、安全性以及切除前内镜超声检查(EUS)的价值。方法 SMT71例中食管36例，胃29例，十二指肠和直肠各3例，64例(90．1％)治疗前行EUS检查。SMT大小6～20mm，平均14．2mm。55例用双活检管道内镜行黏膜切除术(EMR)，把持钳剥离SMT后，将其切除；6例先用圈套器在SMT基底部勒紧，再注入生理盐水，切除SMT；10例≤10mm的用透明帽吸引法切除。结果 71例SMT中68例(95．8％)内镜下完全切除；2例(1例异位胰腺、1例胃平滑肌瘤)病变残留(4周时胃镜发现)；l例直肠平滑肌瘤，未能切除改行外科手术。67例平均随访18．7个月未见复发。组织学诊断平滑肌瘤51例(71．8％)，颗粒细胞瘤、纤维瘤、异位胰腺、脂肪瘤、间质瘤和类癌共15例(21．1％)，5例(7．0％)间叶肿瘤未做免疫染色，不能确定组织来源。并发症：9例局部少量出血，1例胃间质瘤切除后胃穿孔。结论 内镜切除SMT是一种较安全、有效的方法，并可获得组织学诊断，EUS对内镜治疔SMT选择适应证有重要的价值。     

Role of Toll-like receptors in health and diseases of gastrointestinal tract

The human gastrointestinal (GI) tract is colonized by non-pathogenic commensal microflora and frequently exposed to many pathogenic organisms. For the maintenance of GI homeostasis, the host must discriminate between pathogenic and non-pathogenic organisms and initiate effective and appropriate immune and inflammatory responses. Mammalian toll-like receptors (TLRs) are members of the pattern-recognition receptor (PRR) family that plays a central role in the initiation of innate cellular immune responses and the subsequent adaptive immune responses to microbial pathogens. Recent studies have shown that gastrointestinal epithelial cells express almost all TLR subtypes characterized to date and that the expression and activation of TLRs in the GI tract are tightly and coordinately regulated. This review summarizes the current understanding of the crucial dual roles of TLRs in the development of host innate and adaptive immune responses to GI infections and the maintenance of the immune tolerance to commensal bacteria through down-regulation of surface expression of TLRs in intestinal epithelial cells.     

Carbonic anhydrases in normal gastrointestinal tract and gastrointestinal tumours

Carbonic anhydrases (CAs) catalyse the hydration of C02 to bicarbonate at physiological pH. This chemical interconversion is crucial since HCO3- is the substrate for several biosynthetic reactions. This review is focused on the distribution and role of CA isoenzymes in both normal and pathological gastrointestinal (GI) tract tissues. It has been known for many years that CAs are widely present in the GI tract and play important roles in several physiological functions such as production of saliva, gastric acid, bile, and pancreatic juice as well as in absorption of salt and water in intestine. New information suggests that these enzymes participate in several processes that were not envisioned earlier. Especially, the recent reports on plasma membrane-bound isoenzymes IX and XII have raised considerable interest since they were reported to participate in cancer invasion and spread. They are induced by tumour hypoxia and may also play a role in von Hippel-Lindau (VHL)-mediated carcinogenesis.     

Effect of preoperative FOLFOX chemotherapy on CCL20/CCR6 expression in colorectal liver metastases

AIM:To evaluate the influence of preoperative FOLFOX chemotherapy on CCL20/CCR6 expression in liver metastases of stage Ⅳ colorectal cancer(CRC) patients.METHODS:Using Real Time-PCR,enzyme-linked immunosorbent assay,Western Blots and immunohistochemistry,we have analyzed the expression of CCL20,CCR6 and proliferation marker Ki-67 in colorectal liver metastasis(CRLM) specimens from stage Ⅳ CRC patients who received preoperative FOLFOX chemotherapy(n = 53) and in patients who did not receive FOLFOX chemothera...     

自身免疫性肝炎患者血清趋化因子和GP73水平变化及其临床意义研究

目的 探讨自身免疫性肝炎(AIH)患者血清趋化因子和高尔基体糖蛋白73(GP73)水平的变化及其临床意义。方法 2018年3月～2019年3月我院就诊的46例自身免疫性肝炎患者和46例同期健康人群,采用ELIS法检测血清趋化因子C-C-基元受体6和20(CCR6、CCL20)、血清趋化因子配体10(CXCL10)和GP73水平。AIH患者接受肝活检,并将肝炎程度分为轻中重度。结果 AIH血清CCR6、CCL20、CXCL10和GP73水平分别为(112.4±59.3)pg/mL、(186.5±71.8)pg/mL、(81.5±42.0)pg/mL和(171.4±62.5)ng/mL,均显著高于健康人【分别为(75.8±32.6)pg/mL、(123.7±42.2)pg/mL、(53.9±28.1)pg/mL和(83.1±35.2)ng/mL,P<0.05】;10例重度患者血清CCR6、CCL20、CXCL10和GP73水平分别为(174.2±81.4)pg/mL、(271.5±99.7)pg/mL、(162.7±83.1)pg/mL和(278.3±91.5)ng/mL,,显著高于14例中度组【分别为(124.5±55.3)pg/mL、(198.6±66.9)pg/mL、(88.4±46.8)pg/mL和(186.2±75.8)ng/mL,P<0.05】或22例轻度组【分别为(83.5±34.5)pg/mL、(155.6±51.0)pg/mL、(42.6±22.7)pg/mL和(123.9±58.9)ng/mL,P<0.05】;26例肝组织G3～4级患者血清CCR6、CCL20、CXCL10和GP73水平分别为(154.5±28.1)pg/mL、(201.6±56.3)pg/mL、(98.4±56.1)pg/mL和(196.2±59.78)ng/mL,显著高于20例G1～2级组【分别为(73.5±34.8)pg/mL、(135.6±41.7)pg/mL、(62.5±20.1)p g/mL和(93.9±33.9)ng/mL,P<0.05】。结论 AIH患者血清CCR6、CCL20、CXCL10和GP73水平升高,且随着疾病程度和肝组织炎症活动度加重而升高。检测自身免疫性肝炎患者血清趋化因子和GP73水平可能有助于评估患者病情的变化。     

Synchronous occurrence of gastrointestinal stromal tumors and other primary gastrointestinal neoplasms

INTRODUCTION Gastrointestinal stromal tumors (GISTs) are an un-common mesenchymal neoplasm affecting the GI tract. The synchronous occurrence of mesenchymal tumors and other primary gastrointestinal malignancies has been rarely reported in the literature[…     

Heme oxygenase-1 system and gastrointestinal tumors

Heme oxygenase-1(HO-1) system catabolizes heme into three products:carbon monoxide,biliverdin/bilirubin and free iron.It is involved in many physiological and pathophysiological processes.A great deal of data has demonstrated the roles of HO-1 in the formation,growth and metastasis of tumors.The interest in this system by investigators involved in gastrointestinal tumors is fairly recent,and few papers on HO-1 have touched upon this subject.This review focuses on the current understanding of the physiologic...