广州地区1500例健康妇女阴道分泌物检查结果分析

目的了解广州地区健康妇女阴道感染情况。方法用KOH湿片法进行阴道分泌物常规及细菌性阴道病(BV)检查。结果1500例妇女中:阴道清洁度异常比例45.07%;假丝酵母菌阳性52例,阳性率3.47%;阴道毛滴虫阳性3例,阳性率0.20%;细菌性阴道病(BV)66例,阳性率4.40%;假丝酵母菌合并细菌性阴道病(BV)12例(0.80%)。结论现阶段妇女阴道感染情况不容乐观,有必要定期进行阴道分泌物检查。     

广州地区1500例健康妇女阴道分泌物检查结果分析

目的了解广州地区健康妇女阴道感染情况。方法用KOH湿片法进行阴道分泌物常规及细菌性阴道病（Bv）检查。结果1500例妇女中：阴道清洁度异常比例45．07％;假丝酵母菌阳性52例,阳性率3．47％;阴道毛滴虫阳性3例,阳性率0．20％;细菌性阴道病（BV）66例,阳性率4．40％;假丝酵母菌合并细菌性阴道病（BV）12例（0．80％）。结论现阶段妇女阴道感染情况不容乐观,有必要定期进行阴道分泌物检查。     

886例细菌性阴道病的临床诊断和治疗分析

目的:探讨细菌性阴道病(BV)在妇女病普查人群中的患病率及诊断与治疗。方法:回顾性分析2008年3月～2009年12月进行妇女病普查的6 216例妇女细菌性阴道病的临床诊断及治疗结果。结果:6 216例中有886例符合细菌性阴道病诊断标准,占14.25%。其中阴道分泌物阳性占22.4%,pH>4.5的占68.8%,线索细胞阳性率占100.0%。其中65例细菌性阴道病患者阴道分泌物检出假丝酵母菌,14例检出滴虫。采用抗厌氧菌药物治疗效果显著。结论:细菌性阴道病是育龄妇女生殖感染常见疾病之一,应提高认识,及时诊断及治疗。     

1999～2001年永州地区细菌性阴道病调查分析

目的　了解细菌性阴道病的流行情况 ,为控制BV提供科学依据。　方法　采用检测阴道分泌物的pH值、胺试验、G染色查线索细胞的方法诊断细菌性阴道病。　结果　BV的患病率有逐年上升的趋势 (P <0 .0 0 1)。而且以年龄在 2 0～ 2 9岁患病率最高 42 83 %。　结论　应加强对性活跃人群细菌性阴道病的早诊断早治疗     

早孕人工流产妇女阴道分泌物检查结果分析

目的:了解早孕人工流产妇女中细菌性阴道病(BV)、滴虫性阴道炎、念珠菌阴道炎的检出率及其相互关系。方法:对早孕人工流产妇女,取阴道分泌物做生理盐水湿片镜检筛查滴虫性阴道炎,同时将阴道分泌物涂片做革兰染色镜检筛查细菌性阴道病、念珠菌阴道炎。结果:516份阴道分泌物标本中,BV、念珠菌阴道炎、滴虫性阴道炎检出率分别是33·53%、9·11%和4·07%,BV合并念珠菌阴道炎、BV合并滴虫性阴道炎的检出率分别为5·62%和2·13%,BV同时合并念珠菌阴道炎和滴虫性阴道炎检出率1·55%。结论:在早孕人工流产妇女中BV检出率最高。提示临床医生应重视人工流产术前进行常见阴道感染、特别是BV的检查,以避免因漏诊而引起的不良结局。     

1200例不孕妇女阴道分泌物的感染情况分析

目的了解门诊不孕妇女阴道分泌物的病原学及合并感染情况。方法用湿片法进行阴道分泌物及细菌性阴道病(BV)检查;革兰氏染色法检查G-双球菌;用Unipath有限公司沙眼衣原体检测试剂合(免疫层析法)进行衣原体检测;用江门市凯林贸易有限公司支原体选择鉴定培养基进行支原体检测。结果1200例不孕妇女中,其阴道分泌物假丝酵母菌阳性65例,阳性率5.42%;阴道毛滴虫阳性2例,阳性率0.17%;细菌性阴道病(BV)187例,阳性率15.58%;G-双球菌16例,阳性率1.33%。衣原体、支原体阳性共322例,总感染率为26.83%。主要的混合感染:假丝酵母菌+BV:14例(1.17%);UU+BV:11例(0.92%);UU+CT:9例(0.75%)。假丝酵母菌+UU:9例(0.75%)。结论不孕妇女阴道分泌物的病原体构成复杂,UU、CT、BV、和外阴阴道假丝酵母菌病为主要病原体、病因。     

200例细菌性阴道病的检验方法比较

目的就200例细菌性阴道病的检验方法进行比较。方法选取2010—2012年在该院进行细菌性阴道病检测的200例女性患者资料,在细菌性阴道病患者阴道壁下1/3处用无菌棉拭子采集多份分泌物样本,在采样之后第一时间将其送检。采样BV快速检测法、线索细胞检查法、胺试验法、阴道pH值测定法进行检验。结果阴道pH值测定法检验出的阳性为106例(53%),阴性为94例(47%);胺试验法检验出的阳性为112(56%),阴性为88例(44%);线索细胞检查法检验出的阳性为136例(68%),阴性为64例(32%);BV快速检测法检验出的阳性为142例(71%),阴性为58例(29%),四组检验方法差异显著(P<0.05),具有较为明显的统计学意义。结论在细菌性阴道病的检验过程中,Bv快速检测法阳性率高、检测结果清晰、反应过程中不需加热,操作时间段,一般在10min之内就能够全部完成,具有省时、操作简单的突出优点,值得在临床上大力推广。     

不同职业妇女阴道分泌物检测结果分析

目的分析杭州市余杭区不同职业妇女阴道分泌物检查结果,了解阴道清洁度异常率、细菌性阴道病、念珠菌性阴道炎、滴虫性阴道炎在不同人群中的发病率。方法对参加妇女病普查的2639例农村妇女、女员工、女干部,取阴道分泌物做湿片,镜检念珠菌、阴道滴虫、线索细胞及清洁度,做干片行革兰染色,镜检线索细胞、念珠菌、阴道滴虫感染情况。结果农村妇女、女员工、女干部的阴道清洁度异常率分别为:43.4%、39.1%和6.6%;细菌性阴道病发病率分别为:18.9%、16.3%和5.4%;念珠菌感染率分别为:14.7%、15.0%和9.2%;滴虫感染率分别为:6.3%、5.5%和1.1%。农村妇女和女员工组的发病率显著高于女干部组,但该两组的发病率无差异。结论阴道清洁度异常和各种病原体感染在农村妇女和女员工中高发,应加强宣传教育。     

细菌性阴道病加特纳菌检测的临床价值

目的研究超高倍显微镜观察阴道分泌物查线索细胞、分离培养法、胺试验3种检测方法在细菌性阴道病(BV)加特纳菌检测中的临床价值,并进行阴道加德纳菌的药物敏感试验,以指导临床治疗。方法分别用超高倍显微镜观察阴道分泌物查线索细胞,分离培养法,胺试验3种检测方法对208例下生殖道感染患者行细菌性阴道病加德纳菌检测,并进行阴道加特纳菌的药物敏感试验。结果BV、非细菌性阴道病(NBV)患者均经线索细胞检查,BV试验和GV培养,BV患者组三种方法检测到的GV均高于NBV组(P0.05);加特纳菌对万古霉素、菌必治、痢特灵敏感性较高。结论阴道分泌物直接涂片找加特纳菌和线索细胞、细菌分离培养、BV快速诊断等方法在细菌性阴道病(BV)加特纳菌检测中较PCR、免疫荧光法等简便易行。细菌培养及药物敏感试验,对复发性和久治不愈的BV治疗有重要的指导意义。     

基层适宜的阴道分泌物检验技术

阴道分泌物检查的主要目的是诊断女性有无念珠菌性阴道炎、滴虫性阴道炎及细菌性阴道病.国家免费孕前优生健康检查项目中,阴道分泌物检查的内容包括念珠菌、滴虫、pH值、线索细胞、嗅试验、清洁度等.淋球菌、沙眼衣原体主要感染部位为宫颈,其检测取宫颈分泌物进行,故不包含在本文介绍范围.     

A prospective study of genital infections in a family-planning clinic. 1. Microbiological findings and their association with vaginal symptoms

A prospective study of genital infection was conducted in four inner-city family-planning clinics. Fifteen per cent of routine attenders had symptoms and signs of vaginal infection and many more women attended primarily because of symptoms. Among the women with both signs and symptoms, 70% had positive laboratory findings, Trichomonas vaginalis, Candida albicans and bacterial vaginosis being equally prevalent. Measurement of vaginal pH in the clinic was the single most useful clinical finding for directing empirical therapy. Among patients with a discharge confirmed on examination and an abnormally high pH, 72% had either T. vaginalis or bacterial vaginosis. Neisseria gonorrhoeae was isolated from 4% of women with, and 1% of those without, symptoms. We believe that it is worthwhile to investigate patients presenting to family-planning clinics with vaginal symptoms. No single specimen was found ideal for all pathogens, a cervical swab is better for gonococci and also for T. vaginalis but a vaginal swab is needed for candida and bacterial vaginosis.     

Bacterial vaginosis: Prevalence in an Italian population of asymptomatic pregnant women and diagnostic aspects

The present study has been carried out with the main objective of determining the prevalence rate of bacterial vaginosis (BV) in a population of asymptomatic pregnant women (8th to 9th month of pregnancy). Out of a total 1,441 patients examined BV was found to be present in 70 (4.9%). The diagnosis was made when an analysis of the vaginal discharge revealed the simultaneous presence of at least three out of four of the following laboratory indices: (1) direct Gram stain positive (bacterial flora mixed with Gram-negative coccobacilli and variablegram predominant over the lactobacillus flora); (2) pH > 4.5; (3) positive odour test with KOH 10%; (4) presence of clue cells. After a discussion of the principal laboratory and clinical signs presently used for the diagnosis of BV, the authors suggest the exclusion of the evaluation of the appearance of the vaginal discharge from the standard diagnostic criteria. Further, three diagnostic methods are hypothesized for use in different settings.     

线索细胞、pH测定和氨气味试验在细菌性阴道病的诊断价值

目的:探讨线索细胞、pH测定和氨气味试验在细菌性阴道病(BV)的诊断价值。方法:对2007年2～4月在四川大学华西第二医院门诊就诊,符合纳入标准和排除标准的127例妇女盲法进行线索细胞分析、pH测定、氨气味试验和分泌物Nugent积分。以Nugent积分作为BV诊断的"金标准",对线索细胞、pH测定和氨气味试验进行诊断试验性能评价。结果:线索细胞分析具有较高的敏感度(91.49%)和阴性预测值(94.37%),氨气味试验具有较高的特异度(93.75%)和阳性似然比(9.87),而该两项试验的其他诊断性能也较好,pH值试验诊断BV各项性能指标均较差。三项试验的联合诊断敏感度降为38.44%,特异度达99.74%。结论:在BV诊断的Amsel标准中,诊断性能依次为线索细胞、氨气味试验和pH值,多项试验联合应用可提高诊断特异度。     

1895例妇女各类阴道炎病原体感染情况及病因构成

目的:调查妇女阴道炎流行情况,了解不同年龄段妇女阴道炎的致病因子。方法:1895例患者按年龄分为3组,分别对其阴道分泌物进行常规涂片检查、唾液酸酶试验、革兰氏染色镜检及PCR检测等。结果:引起阴道炎的各病原体感染率依次为白色念珠菌28.5%、阴道加德纳菌19.4%、衣原体15.1%、阴道毛滴虫14.4%、淋球菌5.8%、其他4.1%。有263例标本同时合并两种病原体感染。3个年龄组中以18～34周岁组发生阴道炎最多,居首位。各年龄组病原感染位次排除混杂因素,18～34岁组以白色念珠菌感染居首位,其次为衣原体感染;35～50岁组以白色念珠菌和阴道加德纳菌感染为主,其次是阴道毛滴虫。结论:白色念珠菌是引起妇女阴道炎的主要病原体,在临床阴道炎诊治中应注意几种病原体合并感染的情况,各类病原体在不同年龄妇女阴道炎中所占构成比不同,可能与年龄相关的生理因素以及个人行为因素有关。     

阴道分泌物荧光染色法检测病原体的临床应用评价

目的通过比较湿片法和荧光法在阴道分泌物病原体检测中的结果，对阴道分泌物荧光染色的临床价值进行评价。 方法采集140例阴道分泌物标本，分别采用湿片法和荧光法检测标本中的线索细胞、念珠菌和阴道毛滴虫等病原体，并分析其检测结果。 结果湿片法检出线索细胞、念珠菌和阴道毛滴虫的阳性率分别为4.29%、13.57%和2.86%，荧光法的阳性率分别为11.43%、15.00%和2.86%；两种方法在检出线索细胞方面的差异有统计学意义（P＜0.05），在检测阴道毛滴虫和念珠菌的阳性率方面一致。 结论荧光染色检测检测线索细胞的阳性率高于湿片法，可用于临床阴道分泌物病原体的检测。     

A study of field-based methods for diagnosing reproductive tract infections in rural Yunnan Province, China

Reproductive tract infections are a significant cause of poor health among rural Chinese women. This study assesses the accuracy of simple, field-based methods of diagnosing five reproductive tract infections (trichomonas, candida, bacterial vaginosis, gonorrhea, and chlamydia) in two rural Chinese counties, using "gold standard" laboratory diagnosis as the reference. Self-reported symptoms and clinical examination proved inadequate for making accurate diagnoses, because many infected women show no symptoms or are infected with two or more conditions. The use of simple tests such as KOH staining, wet mount, Gram's stain, and pH of discharge greatly improved diagnostic accuracy, especially for candida and trichomonas, the two most common infections. Promoting the use of such diagnostic approaches by rural Chinese medical and family planning health providers would reduce the prevalence and severity of these preventable and easily treated infections.     

The impact of regular vaginal pH screening on the diagnosis of bacterial vaginosis in pregnancy

BACKGROUND: Bacterial vaginosis has recently been associated with preterm labor and delivery. The purpose of our study was to determine whether regular prenatal vaginal pH testing resulted in more frequent diagnoses of bacterial vaginosis and other vaginal infections, more frequent treatment with antibiotics, and fewer preterm deliveries. We also sought to determine the sensitivity and specificity of pH testing and vaginal symptom reporting in identifying vaginal infections. METHODS: Our study was a prospective clinical trial involving 121 pregnant women randomized to receive either standard prenatal care, including routine inquiry about vaginal symptoms, or standard care supplemented by vaginal pH testing. Women with symptoms or a vaginal pH level >4.5 received a wet mount examination. Confirmed infections were treated according to study protocols. RESULTS: Women who received regular pH testing showed significantly higher detection rates for bacterial vaginosis than controls (48.4% vs 27.1%, P =.015) and more frequent detection of Trichomonas vaginalis (7.8% vs 1.7%, P = .116). A higher percentage of women in the experimental group were treated for bacterial vaginosis and trichomoniasis (46.9% vs 27.1%, P =.024), and the preterm birth rate was one half that of the control group (4.7% vs 10.2%, P = .243). The presence of vaginal symptoms or a vaginal pH level >4.5 identified bacterial vaginosis or trichomoniasis with 84.4% sensitivity. CONCLUSIONS: In our study, frequent vaginal pH testing during pregnancy resulted in more frequent diagnosis and treatment of bacterial vaginosis. Since vaginal symptoms and elevated pH levels appear to be useful in screening for bacterial vaginosis and trichomoniasis, frequent pH testing should be evaluated in larger studies.     

社区卫生门诊白带检查结果分析

目的：了解某地区社区卫生门诊妇女阴道炎患者的发病情况及白带的微生态状况.方法：用直接湿片涂片镜检、涂片革兰染色镜检、胺试验方法对患者白带的pH值、细菌、霉菌、滴虫进行检查分析.结果：1734例患者检出细菌性阴道炎574例（33.1%）,念珠菌病388例（22.4%）,滴虫病120例（6.9%）,淋病24例（1.4%）,胺试验阳性475例（27.4%）,pH〉5.0者492例（28.4%）.结论：某地区社区卫生门诊妇女阴道炎中细菌性阴道病阳性率远高于真菌、滴虫感染,应加强对妇女的卫生健康教育及细菌性阴道病感染的临床诊断和治疗.