超声引导下细针抽吸睾丸组织与粗针活检的比较

目的　探讨超声引导下细针抽吸睾丸组织的诊断价值。 方法　回顾分析2015年1月到2016年12月的睾丸细、粗针穿刺活检病理切片,比较其中生精小管断面数量的差异;除生精小管以外的其他成分（皮肤、血管及纤维软组织）及血肿的差异。 结果　粗针穿刺与细针抽吸睾丸组织病理切片,生精小管断面数量大于20的,粗针穿刺组有8例(8/24,33.3%),细针抽吸组有11例(11/20,55.0%),组间对比（χ2=2.087,P=0.149）,两者差异无统计学意义;存在其他成分例数,粗针穿刺组有19例( 19/24,79.2%);细针抽吸组有1例(1/20,0.9%),组间对比(χ2 =24.203,P=0.000),两者差异有统计学意义;穿刺后出现血肿例数,粗针穿刺组有5例(5/24,20.8%),而细针抽吸组20例均未见血肿,组间对比（χ2=4.70,P=0.032）,两者差异有统计学意义。 结论　细针抽吸睾丸组织标本满意,几乎无其他成分干扰,具有较大的临床应用价值。     

超声引导下甲状腺结节细针活检联合甲状腺良性结节射频消融的临床应用价值

目的探讨超声引导下甲状腺结节细针活检联合甲状腺良性结节射频消融(RFA)的临床应用价值。方法在细针活检确诊150例甲状腺结节患者中,选择70例甲状腺结节病(160枚),随机分为组1(n=35,80枚)采用传统手术方法治疗;组2(n=35,80枚),采用超声引导下射频消融治疗,比较两组治疗效果和并发症情况。结果 150例超声引导下的甲状腺细针穿刺活检144例与术后病理符合。组2治疗总有效率96.8%(61/63),高于组1的79.4%(50/63),差异具有统计学意义(P0.05);组2并发症发生率3.7%(1/27)低于组1的33.3%(9/27),组间差异具有统计学意义(P0.05)。结论超声引导下细针活检联合热消融甲状腺良性结节治疗效果好,并发症少。     

彩超引导下甲状腺结节粗针穿刺活检的诊断意义

目的 评价彩超引导下粗针穿刺活检在甲状腺结节诊断方面的临床意义.方法 对77例甲状腺结节患者在彩超引导下行甲状腺结节粗针穿刺活检.结果 77例经皮穿刺甲状腺成功率97.4%(75/77),无并发症.临床和病理符合率为100%.结论 彩超引导下甲状腺结节粗针穿刺活检是一种安全、简便、可行的术前确诊手段.     

超声造影联合Galectin-3检测对Bethesda Ⅲ类甲状腺结节性质诊断价值评估

目的 分析细针穿刺细胞学检查(FNAC)Bethesda Ⅲ类甲状腺结节结合组织Galectin-3分子表达和超声造影(CEUS)在鉴别良恶性中的应用价值.方法 选择行FNAC检查诊断为Bethesda Ⅲ类甲状腺结节患者110例(140个结节),其中男性52例,女性58例;年龄25～64岁,平均年龄45.5岁.所有组...     

超声引导下甲状腺细针穿刺细胞学检查及液基薄层细胞学在甲状腺疾病诊断中的应用价值

目的探讨超声引导下甲状腺细针穿刺细胞学及液基薄层细胞学对甲状腺疾病诊断的临床应用价值。方法选择78例甲状腺结节患者,其中男性34例,女性44例;年龄33~64岁,平均年龄47.6岁。对甲状腺结节患者行细针穿刺活组织检查,观察细胞病理学与组织病理学诊断结果。结果 78例患者均获取足够的标本,其中73例(93.6%)细胞学结果与病理学诊断结果一致,即20例(25.6%)为恶性肿瘤,44例(56.4%)为良性肿瘤,9例(11.5%)为非肿瘤性病变。甲状腺细针穿刺鉴别甲状腺结节良恶性的灵敏度为90.9%,特异度为98.1%。阳性预测值为96.3%。结论超声引导下甲状腺细针穿刺细胞学检查及液基薄层细胞学检查在甲状腺疾病诊断中有诊断性的临床应用价值,与组织病理学检查总体诊断符合率较好。该方法为是否采取手术治疗及术式的选择提供了有力的依据,是一种安全、有效的术前确诊手段。     

甲状腺癌的超声诊断及相关分子标记物检测的研究进展

随着超声成像技术的发展,甲状腺结节的检出率日益提高,但病灶的良恶性鉴别在声像图上仍缺乏独有的特征性.以结节的常规超声征象为基础,应用甲状腺影像报告和数据系统对结节进行正确分级,有利于甲状腺结节的鉴别诊断.超声造影(CEUS)技术能显示病灶的微血管状态,通过观察不同的增强模式分析甲状腺结节的良恶性;超声弹性成像(UE)技术则通过评价不同组织间的硬度差异,为鉴别甲状腺良恶性结节提供了补充方法;超声引导下细针穿刺抽吸活检(US-FNAB)能准确定位,并为甲状腺结节的术前诊断提供病理依据.对于部分难以明确诊断的穿刺标本进行分子标记物检测则有助于术前诊断甲状腺癌.就甲状腺癌的超声诊断及相关分子标记物检测的研究进展进行综述.     

液基细胞学技术在甲状腺结节细针穿刺病理诊断中的应用

<正>甲状腺结节为临床常见疾病,随着高分辨率超声的普及应用,甲状腺结节的检出率可达20%~76%~([1]),其中恶性结节占5%~6.5%~([2])。鉴别结节的良恶性一直是临床上的难点,超声引导下细针穿刺活检(ultrasonic guidance fine-needle aspiration biopsy,UG-FNAB)是一种简便、安全、微创的细胞学检查方法,被国内外公认为是诊断甲状腺结节性质的首选方法 ~([3-4])。液基薄层细胞学检查(thinprep cytologic test,TCT)广泛应用于妇科宫颈脱落细胞学检测,在非妇科尤其是甲状腺细针穿刺细胞学检查中的应用报道较少。本实验     

C-TIRADS结合超声弹性成像诊断甲状腺囊实性结节良恶性的价值

目的 探讨中国版甲状腺影像报告和数据系统（C-TIRADS）与应变弹性成像技术相结合对甲状腺囊实性结节良恶性的鉴别诊断价值。方法 选择行甲状腺超声检查的80例患者（91个囊实性结节），其中男性27例，女性53例；年龄17～72岁，平均年龄47.8岁；结节最大径线5～61 mm，平均结节最大径线27.9 mm。回顾性分析91个甲状腺囊实性结节的二维灰阶超声图像及弹性声像图，运用C-TIRADS进行分类，比较单独使用C-TIRADS分类方法、应变弹性成像技术及两种方法结合对甲状腺囊实性结节的诊断效能。结果 91个甲状腺囊实性结节中良性结节69个（75.8%），包括结节性甲状腺肿37个（53.6%），滤泡性腺瘤囊性变7个（10.1%），穿刺良性结节25个（36.2%）；恶性结节22个（24.2%），均为乳头状癌。单独使用C-TIRADS、应变弹性成像技术诊断甲状腺囊实性结节的灵敏度、特异度、准确度分别为45.5%、95.7%、86.8%及59.1%、97.1%、89.0%，两种方法结合诊断甲状腺囊实性结节的灵敏度、特异度、准确度为81.8%、94.2%、91.2%，联合诊断可提高甲状腺囊实性...     

应变率弹性成像及剪切波弹性成像对TI-RADS 4类 甲状腺结节的诊断价值对比

目的 对比分析应变率弹性成像（SRE）及剪切波弹性成像（SWE）在TI-RADS 4类甲状腺结节中的应用价值。方法 对80例TI-RADS 4类甲状腺结节行细针活检穿刺,术前对结节均行SRE及SWE检查,根据病理结果对比分析两者对4类甲状腺结节的诊断价值。结果 80例结节中,经病理证实甲状腺乳头状癌46例,SRE敏感性及准确性分别为86.95%、59.70%;SWE敏感性及准确性分别为89.13%、64.06%,SRE联合SWE敏感性及准确性分别为89.74%、85.37%,SRE及SWE诊断准确性方面差异无统计学意义,SRE+SWE准确性高于单独应用SRE或SWE。结论 SWE对甲状腺4类结节诊断准确性略高于SRE,两者联合有助于提高4类甲状腺结节的准确性。     

孤立性肺实性结节和混杂磨玻璃结节对比研究

目的　探讨孤立性实性和混杂磨玻璃结节的病理特征,找出它们之间的影像学特征差异。 方法 回顾性分析804例孤立性肺结节（SPNs）患者手术切除治疗后的病理特征。根据胸部高分辨CT扫描结果是否存在磨玻璃样病变,将SPN分为实性和混杂磨玻璃结节。比较和评估实性和混杂磨玻璃结节组之间的病理特征。获得人口统计学（性别和年龄）和临床数据（吸烟,癌症史,糖尿病史,慢性阻塞性肺气肿或肺纤维化病史,肺结节的最大直径和位置）,分析实性和混杂磨玻璃结节之间的差异。 结果 804例SPN患者中,48.9%（393例）为良性SPNs,51.1%（ 411例）为恶性;67.7%（544例）为实性结节,32.3%（260例）为混杂磨玻璃结节。在实性结节组中,61.2%（333例）被诊断为良性,38.8%（ 211例）为恶性。在混杂磨玻璃结节组中,23.1%（60例）为良性,76.9%（200例）为恶性。实性结节中良性病变的比例显著高于混杂磨玻璃结节中的比例（P<0.001）。此外,实性和混杂磨玻璃结节之间腺癌的亚型有显著差异（P<0.001）。混杂磨玻璃结节组中女性患者,慢性阻塞性肺气肿或肺纤维化病史患者,非吸烟者和上叶位置的比例远高于实性结节组。 结论　CT表现为实性和混杂磨玻璃结节恶性病变中,腺癌是最常见组织学类型,在腺癌亚型的构成上两者之间存在明显的差异。与男性和吸烟者相比,女性和非吸烟者（被动吸烟者）更倾向于发展为与肺腺癌相关的混杂磨玻璃结节,在大多数情况下,混杂磨玻璃结节位于肺的上叶。     

Techniques for thyroid FNA: a synopsis of the National Cancer Institute Thyroid Fine-Needle Aspiration State of the Science Conference

The National Cancer Institute (NCI) sponsored the NCI Thyroid fine-needle aspiration (FNA) State of the Science Conference on October 22-23, 2007 in Bethesda, MD. The 2-day meeting was accompanied by a permanent informational website and several on-line discussion periods between May 1 and December 15, 2007 (http://thyroidfna.cancer.gov). This document summarizes matters addressing manual and ultrasound guided FNA technique and related issues. Specific topics covered include details regarding aspiration needles, devices, and methods, including the use of core needle biopsy; the pros and cons of anesthesia; the influence of thyroid lesion location, size, and characteristics on technique; the role of ultrasound in the FNA of a palpable thyroid nodule; the advantages and disadvantages of various specialists performing a biopsy; the optimal number of passes and tissue preparation methods; sample adequacy criteria for solid and cystic nodules, and management of adverse reactions from the procedure. (http://thyroidfna.cancer.gov/pages/info/agenda/)     

Analysis of the bethesda system for reporting thyroid cytopathology and similar precursor thyroid cytopathology reporting schemes

The Bethesda System for Reporting Thyroid Cytopathology is a standardized reporting system for classifying thyroid fine-needle aspiration results comprising of 6 diagnostic categories with unique risks of malignancy and recommendations for clinical management. The majority of thyroid nodules are benign; however, up to 30% of fine-needle aspiration of thyroid nodule results are equivocal. Until 2007, various diagnostic terms were used to classify such cases, including "atypical," "indeterminate," and rule-out or cannot exclude malignancy. A literature review of 13 original studies was conducted to evaluate whether utilization of the Bethesda System for Reporting Thyroid Cytopathology nomenclature represent an improvement over thyroid cytopathology reporting schemes used before 2007 in diagnosing thyroid malignancy. The sensitivity and specificity of thyroid fine-needle aspiration was high in the studies that assessed the measures. However, a selection bias exists and most studies do not include indeterminate diagnosis in their calculations. Although the Bethesda System for Reporting Thyroid Cytopathology recommends a repeat fine-needle aspiration to follow-up nondiagnostic specimens, in the majority of studies, an appreciable number of cases underwent follow-up surgical biopsy or thyroidectomy. The diagnostic category of atypia/follicular lesion of undetermined significance remains heterogenous in terms of usage and clinical outcome. The majority of the studies that utilize the Bethesda System for Reporting Thyroid Cytopathology in this literature review retrospectively reclassified thyroid fine-needle aspiration into the Bethesda System for Reporting Thyroid Cytopathology nomenclature with reported malignancy rates that are similar between cases reclassified as atypia/follicular lesion of undetermined significance and follicular neoplasm/suspicious for follicular neoplasm.     

Fine needle aspiration biopsy of thyroid nodules: cytologic and histopathologic correlation of 1096 patients

Via routinely used thyroid function tests, scintigraphy and ultrasonography (USG), important information is obtained in the clinical and diagnostic practice for thyroid nodules. However, the distinction between benign and malignant lesions cannot precisely be performed with these tests. Thyroid fine needle aspiration biopsy (FNAB) is considered the most reliable diagnostic method in the differentiation between benign and malignant thyroid nodules. It has recently been likely to perform aspiration from deeper nodules via the implemention of FNAB along with USG. Today, in cytopathological examination of thyroid FNAB, standardized Bethesda System for Reporting Thyroid Cytopathology (BSRTC) system is used. Here, FNAB was performed in 1096 patients with thyroid nodules in the Medical School of Selcuk University between January 2009 and July 2014. Patients consisted of 919 women and 177 men between 12 and 87 years of age. Evaluated via BSRTC, the results were classified as unsatisfactory, benign, atypia (or follicular lesions) of undetermined significance (AUS), follicular neoplasm or lesions suspicious for follicular neoplasm (FN), suspected malignant and malignant. After FNAB, 183 patients were operated and evaluated histopathologically. Histological malignancy rates of the categories were as follows: 16% (5), 15% (6) 14% (1) 60% (9), 72% (18) and 97% (63), respectively. In our study, we aimed to compare FNAB results of thyroid nodules with histopathology results after thyroidectomy and to show the sensitivity and specificity of FNAB technique to be higher in the follow-up and diagnosis of thyroid lesions. Given the malignancy detection rate in the follow-up of patients whose cytology was reported as inadequate, we also consider follow-ups are important in these patients.     

Ultrasound-guided fine-needle aspiration of thyroid nodules: does the size limit its efficiency?

Purpose: The management criterion of thyroid nodules is to evaluate the risk of malignancy, based on cytological examinations. Ultrasound-guided fine-needle aspiration biopsy (US-FNAB) has a highly diagnostic value for thyroid nodules. The aim of this study was to compare the efficacy of US-FNAB for thyroid nodules with different sizes. Material and methods: From August 2013 to November 2013, 344patients with thyroid nodules who had undergone US-FNAB were divided into three groups, according to the largest diameter of their nodules (group A, ≤ 5.0 mm; group B, 5.1-10.0 mm; group C, > 10.0 mm). All the nodules were subsequently verified by histology or follow-up findings. The accuracy, sensitivity, specificity, positive predictive value, negative predictive value of aspiration cytology in each group was compared. Results: Among 344 thyroid nodules diagnosed by cytology, the cytology was classified as nondiagnostic or unsatisfactory for 53 (15.4%) lesions, benign for 144 (41.9%) lesions, atypia of undetermined significance or follicular lesion of undetermined significance for 20 (5.8%) lesions, follicular neoplasm or suspicious for a follicular neoplasm for 26 (7.6%) lesions, suspicious for malignancy for 36 (10.5%) lesions, malignant for 65 (18.9%) lesions. There were 243 benign and 101 malignant nodules confirmed by the pathological or follow-up ultrasound. The sensitivity, specificity, accuracy, positive predictive value, and negative predictive value were confirmed to be 87.5% (14/16), 92.5% (37/40), 91% (51/56), 82.3% (14/17), and 94.8% (37/39) in group A; 92.3% (36/39), 96.9% (94/97), 95.5% (130/136), 92.3% (36/39), and 96.9% (94/97) in group B; and 91.3% (42/46), 93.4% (99/106), 92.7% (141/152) 85.7% (42/49), and 96.1% (99/103), in group C. There were no statistical differences in accuracy, sensitivity, specificity, false positive accuracy, false negative rate of fine needle aspiration of thyroid nodules with different sizes (P > 0.05). Conclusion: US-FNAB has similar diagnostic efficacy to thyroid nodules with different sizes.     

Comparison of Consecutive Results from Fine Needle Aspiration and Core Needle Biopsy in Thyroid Nodules

There are papers suggesting the complementary role of core needle biopsy (CNB) in the diagnosis of thyroid nodules. By comparing the result of CNB and fine needle aspiration (FNA) cytology performed in consecutive cases of thyroid nodules, the role of CNB was evaluated. Retrospective reviews of 2131 FNA and 275 CNB which were performed as first-line biopsy for 2406 thyroid nodules in 2187 patients were performed. The ultrasound (US) feature of thyroid nodule was classified following the risk of malignancy suggested by American Thyroid Association (ATA) guideline. Rate of unsatisfactory and cellular atypia could be decreased significantly by first-line CNB in all US group, and the nodules with highly suspicious feature showed significant decrease in inconclusive result by first-line CNB. However, increased rates of architectural and follicular neoplasm (FN) were identified in CNB group especially in intermediate and low suspicious nodules, and the first-line CNB could not decrease the inconclusive result in these US groups. The diagnostic rate of neoplasm diagnosed by surgery following the result of architectural atypia or FN was not different between FNA and CNB even with significantly higher rate in CNB group. Furthermore, the sensitivity for follicular neoplasm (21.2 vs. 61.9%) was significantly higher in CNB group. The CNB can be considered in nodules with highly suspicious feature with advantage of significantly lower inconclusive diagnostic rate than FNA group. However, significantly increased diagnosis of architectural atypia or FN in other nodules by CNB is recognized and should be evaluated in future to understand the meaning.     

跟骨内侧定位载距突螺钉导向器的研制与初步评价

目的　探讨从跟骨内侧定位、经载距突置入导向针后,由跟骨外侧壁置入载距突螺钉的可行性及精确度,为临床手术方案设计及导向器械的研发改进提供参考依据。 方法　选取10具20侧成人尸体标本,在跟骨外侧做“L”形延长切口,暴露跟骨外侧结构及距下关节,采用自主研发的“跟骨内侧定位载距突螺钉导向器”辅助载距突螺钉置入。首先,从内侧将“定位针”置入距下中关节,将导向器“内侧臂”的“定位孔”套牢定位针,调整“内侧臂”使“导向通道”位于载距突中心,安装“外侧臂”与“手柄”固定。调整“外侧臂”位置,经“导向通道”从内向外穿入跟骨一枚“导向针”,C臂透视提示“导向针”位置理想后,自跟骨外侧壁沿“导向针”用空心钻钻孔,测深后拧入合适的载距突螺钉。最后行CT扫描,利用CT图像评估置入螺钉的精确度。过程中采集相关数据,评估导向器的功能。 结果　肉眼观察标本,所有的导向针位于载距突内,其中65%位于载距突中心,25%偏下方,10%偏后方。在内侧,75%的导向针穿过胫后肌腱,15%位于胫后肌和趾长屈肌腱之间,10%穿经趾长屈肌腱上1/3。在外侧,导向针出针点与跟骰关节的距离为（38.03±5.60）mm,与后关节面的距离为（15.01±3.38）mm,螺钉平均长度为（44.80±3.59）mm。CT扫描图像可见,所有螺钉位于载距突内,有2例穿透上方骨皮质,无螺钉穿出载距突下方、前方及后方骨皮质。 结论　采用跟骨内侧定位法,在自主设计的导向器引导下,可提高载距突螺钉置入的准确性,降低螺钉进入关节、跟骨内侧皮质等并发症。     

跟骨内侧定位载距突螺钉导向器的研制与初步评价

目的　探讨从跟骨内侧定位、经载距突置入导向针后,由跟骨外侧壁置入载距突螺钉的可行性及精确度,为临床手术方案设计及导向器械的研发改进提供参考依据。 方法　选取10具20侧成人尸体标本,在跟骨外侧做“L”形延长切口,暴露跟骨外侧结构及距下关节,采用自主研发的“跟骨内侧定位载距突螺钉导向器”辅助载距突螺钉置入。首先,从内侧将“定位针”置入距下中关节,将导向器“内侧臂”的“定位孔”套牢定位针,调整“内侧臂”使“导向通道”位于载距突中心,安装“外侧臂”与“手柄”固定。调整“外侧臂”位置,经“导向通道”从内向外穿入跟骨一枚“导向针”,C臂透视提示“导向针”位置理想后,自跟骨外侧壁沿“导向针”用空心钻钻孔,测深后拧入合适的载距突螺钉。最后行CT扫描,利用CT图像评估置入螺钉的精确度。过程中采集相关数据,评估导向器的功能。 结果　肉眼观察标本,所有的导向针位于载距突内,其中65%位于载距突中心,25%偏下方,10%偏后方。在内侧,75%的导向针穿过胫后肌腱,15%位于胫后肌和趾长屈肌腱之间,10%穿经趾长屈肌腱上1/3。在外侧,导向针出针点与跟骰关节的距离为（38.03±5.60）mm,与后关节面的距离为（15.01±3.38）mm,螺钉平均长度为（44.80±3.59）mm。CT扫描图像可见,所有螺钉位于载距突内,有2例穿透上方骨皮质,无螺钉穿出载距突下方、前方及后方骨皮质。 结论　采用跟骨内侧定位法,在自主设计的导向器引导下,可提高载距突螺钉置入的准确性,降低螺钉进入关节、跟骨内侧皮质等并发症。     

Ultrasonographic thyroid findings suspicious for malignancy

Asymptomatic incidental thyroid nodules (thyroid incidentalomas) are found in up to a third of the adult population. There is notable overlap in the sonographic appearance of benign and malignant thyroid nodules. This paper provides a brief review of the ultrasound findings of thyroid nodules that are suspicious for malignancy with pathologic correlates. We then discuss the standard approach to a fine needle aspiration biopsy of a thyroid nodule at our institution. Finally, we review specific diagnostic challenges in image guided fine needle aspiration biopsies. Diagn. Cytopathol. 2013;41:1107–1114. © 2013 Wiley Periodicals, Inc.     

Cost-effectiveness of fine-needle-aspiration cytology of thyroid nodules with intranodular vascular pattern using two different needle types

Objective: To compare the cytological findings of hypoechoic thyroid nodules with intranodular vascular pattern (pattern II) obtained by two different needles (Neolus 25 gauge, Chemil, Wenzhou, China vs Yale Spinal 25 gauge, Becton Dickinson, Madrid, Spain) in euthyroid patients and to evaluate their cost-effectiveness. Methods: From January 2001 to December 2003, 480 euthyroid patients with a hypoechoic thyroid nodule pattern II were referred for US-FNAC. The nodules were alternatively evaluated by Neolus or by Yale Spinal with the stylet (YS+) or without the stylet (YS−), in order to evaluate if the cytological results could be due to the presence of the stylet or to the different length of the two needles. For each nodule two passes were performed and the material was obtained by capillary action. Material was smeared on slides, fixed, and stained by Papanicolaou techniques. Cytological specimens were evaluated in blind by the same experienced cytopathologist. Results: Inadequate cytological specimens because of blood contamination were present in 30 (18.7%) samples by Neolus needle and in 22 (13.8%) by YS− compared to only 5 (3.1%) by YS+. In 6 (20%) cases of the 30 repeated US-FNAC by Neolus and in 4 (18%) of the 22 US-FNAC by YS−, material remained inadequate for diagnosis because of blood contamination. All the five repeated samples obtained by YS+ became adequate for diagnosis and resulted benign nodules. Direct costs of US-FNAC procedure are currently Э 72.30 including cytological examination. The cost of Neolus and Yale needles is Э 0.19 and Э 3.0, respectively. The estimated total cost to obtain a cytological diagnosis by a Neolus needle (160 + 30 repeated US-FNAC) was Э 13809.2 vs Э 12919.5 by Yale Spinal needle (160 + 5 repeated US-FNAC). Conclusion: This study demonstrates that the use of Yale Spinal needles greatly reduces inadequate cytological specimens, and therefore limits both direct and indirect costs.     

BRAF(V600E) mutation analysis of liquid-based preparation-processed fine needle aspiration sample improves the diagnostic rate of papillary thyroid carcinoma

Early detection and diagnosis of papillary thyroid carcinoma are important for successful management of patients. Liquid-based preparations (Thinprep) of fine needle aspirations from thyroid nodules are now widely used and are replacing conventional smears because residual samples can be used for ancillary tests. Detection of the BRAF(V600E) mutation in cytology specimens could aid in the diagnosis of papillary thyroid carcinoma. We, therefore, analyzed the cytologic features and BRAF(V600E) mutation status of thyroid liquid-based preparation-fine needle aspiration samples. A total of 191 histologically confirmed thyroid liquid-based preparation-fine needle aspiration specimens were selected. We analyzed cytomorphological features and BRAF(V600E) mutation status in both liquid-based preparation-fine needle aspiration samples and the corresponding formalin-fixed, paraffin-embedded tissues. The Seeplex BRAF ACE detection kit (Seoul, Korea), melting curve analysis with SYBR green, and sequencing analysis were used to detect BRAF(V600E) mutations. Of 191 patients, 126 had histologically confirmed papillary thyroid carcinoma, whereas the remaining 65 lesions were benign lesions and carcinomas of other types. The sensitivity of liquid-based preparation alone for diagnosis of papillary thyroid carcinoma was 71.4%. When BRAF(V600E) mutation analyses results were considered in conjunction with the cytologic diagnosis, the diagnostic sensitivity for detecting papillary thyroid carcinoma increased to 84.9% regardless of the method used to detect BRAF mutations. BRAF(V600E) mutation analysis using residual liquid-based preparation cytologic samples is, therefore, a powerful additional diagnostic tool for diagnosis of papillary thyroid carcinoma.