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1.
Diagnosis of peripheral nerve sheath tumors around the pelvis 总被引:1,自引:0,他引:1
Ogose A Hotta T Morita T Higuchi T Umezu H Imaizumi S Hatano H Kawashima H Gu W Endo N 《Japanese journal of clinical oncology》2004,34(7):405-413
OBJECTIVE: To distinguish between benign and malignant peripheral nerve sheath tumors around the pelvis. METHODS: A retrospective study of 30 patients with benign and malignant peripheral nerve sheath tumors located around the pelvis was performed. Clinical, imaging and histological features of 19 benign and 11 malignant peripheral nerve sheath tumors around the pelvis were reviewed retrospectively. RESULTS: Nearly all patients exhibited pain at presentation in cases involving both benign and malignant tumors. Although tumor size, duration of symptoms and presence of sensory disturbance possessed little value in differential diagnosis, severe motor weakness was observed exclusively in patients presenting with malignant tumors. On CT or MRI, central enhancement was apparent in 11 of the 19 benign tumors; in contrast, central enhancement was evident in one of the 11 malignant tumors. Fine needle aspiration cytology was performed in 11 tumors; correct diagnosis was achieved in four tumors. Core needle biopsy was performed in five tumors, all of which were correctly diagnosed with no neurological deficits. Immunohistochemically, all benign tumors were diffusely positive for S-100 protein, whereas malignant tumors were negative or focally positive for S-100 protein. Ki-67 index was less than 4% in all benign tumors; additionally, this index was 7-36% in malignant tumors. CONCLUSION: Central enhancement pattern on imaging studies strongly suggests a benign tumor; in contrast, severe motor weakness suggests malignant lesions. Core needle biopsy was reliable with respect to preoperative diagnosis. 相似文献
2.
Induction of abnormal proliferation by nonmyelinating schwann cells triggers neurofibroma formation 总被引:3,自引:0,他引:3
Recent evidence suggests that alterations in the self-renewal program of stem/progenitor cells can cause tumorigenesis. By utilizing genetically engineered mouse models of neurofibromatosis type 1 (NF1), we demonstrated that plexiform neurofibroma, the only benign peripheral nerve sheath tumor with potential for malignant transformation, results from Nf1 deficiency in fetal stem/progenitor cells of peripheral nerves. Surprisingly, this did not cause hyperproliferation or tumorigenesis in early postnatal period. Instead, peripheral nerve development appeared largely normal in the absence of Nf1 except for abnormal Remak bundles, the nonmyelinated axon-Schwann cell unit, identified in postnatal mutant nerves. Subsequent degeneration of abnormal Remak bundles was accompanied by initial expansion of nonmyelinating Schwann cells. We suggest abnormally differentiated Remak bundles as a cell of origin for plexiform neurofibroma. 相似文献
3.
Schmidt H Taubert H Meye A Würl P Bache M Bartel F Holzhausen HJ Hinze R 《Cancer letters》2000,155(2):181-190
In order to investigate typical genomic alterations in patients with Recklinghausen's disease (NF1) we studied one from each of the six patients with NF1 several benign and/or malignant tumors. By means of comparative genomic hybridization (CGH) gained results from six benign neurofibromas and 14 malignant peripheral nerve sheath tumors (MPNSTs) were compared with four benign peripheral nerve sheath tumors (BPNSTs) from patients without NF1. In all 14 MPNSTs DNA sequence copy number changes were detected with a mean value of 13.5 imbalances per sample. The most frequent gains were in 8q, 17q (12 tumors each), 7p, 15q (ten tumors each), and 7q (nine tumors). We found ten high-level amplifications in nine of the 14 samples. In two cases, the high-level amplification involved 7p14-pter and 17q24-qter as well. The most frequent loss was in 17p (seven tumors). The benign neurofibromas from NF1-patients and the sporadic BPNSTs revealed only partially DNA sequence copy number changes without any distinct pattern. The gains of #7, 8q, 15q, and 17q were found exclusively in MPNSTs but not in neurofibromas and are supposed to be associated with malignant tumor progression. In comparison of the results of the 14 MPNSTs from NF1-patients with the results of previously published 20 sporadic MPNSTs, we found that the gain of 8q occurs most frequently in both tumor groups. Of course additionally in the sporadic MPNSTs there were more frequent gains of 5p, #6, and statistically significant gains of 20q. On the other hand in the MPNSTs from NF1-patients the most frequent gains were found in #7, and statistically significant in 15q, and 17q. 相似文献
4.
5.
Kiran K Mantripragada Gillian Spurlock Lan Kluwe Nadia Chuzhanova Rosalie E Ferner Ian M Frayling Jan P Dumanski Abhijit Guha Victor Mautner Meena Upadhyaya 《Clinical cancer research》2008,14(4):1015-1024
PURPOSE: Neurofibromatosis type 1 (NF1) is an autosomal dominant condition that predisposes to benign and malignant tumors. The lifetime risk of a malignant peripheral nerve sheath tumor (MPNST) in NF1 is approximately 10%. These tumors have a poor survival rate and their molecular basis remains unclear. We report the first comprehensive investigation of DNA copy number across multitude of genes in NF1 tumors using high-resolution array comparative genomic hybridization (CGH), with the aim to identify molecular signatures that delineate malignant from benign NF1 tumors. EXPERIMENTAL DESIGN: We constructed an exon-level resolution microarray encompassing 57 selected genes and profiled DNA from 35 MPNSTs, 16 plexiform, and 8 dermal neurofibromas. Bioinformatic analysis was done on array CGH data to identify concurrent aberrations in malignant tumors. RESULTS: The array CGH profiles of MPNSTs and neurofibromas were markedly different. A number of MPNST-specific alterations were identified, including amplifications of ITGB4, PDGFRA, MET, TP73, and HGF plus deletions in NF1, HMMR/RHAMM, MMP13, L1CAM2, p16INK4A/CDKN2A, and TP53. Copy number changes of HMMR/RHAMM, MMP13, p16INK4A/CDKN2A, and ITGB4 were observed in 46%, 43%, 39%, and 32%, respectively of the malignant tumors, implicating these genes in MPNST pathogenesis. Concomitant amplifications of HGF, MET, and PDGFRA genes were also revealed in MPNSTs, suggesting the putative role of p70S6K pathway in NF1 tumor progression. CONCLUSIONS: This study highlights the potential of array CGH in identifying novel diagnostic markers for MPNSTs. 相似文献
6.
Toshiki Uchida Tomohiro Kinoshita Takashi Murate Hidehiko Saito Tomomitsu Hotta 《Leukemia & lymphoma》1998,29(1):27-35
p16INK4A is a cyclin-dependent kinase inhibitor (CDKI), and regulates the cell cycle negatively. Recently, p16INK4A protein was shown to be encoded by the CDKN2 gene, which is identical to multiple tumor suppressor gene 1 (MTS1) on chromosome 9p21, where genetic alterations occur frequently in many malignant tumors. As the loss of p16INK4A function by genetic alterations leads to inappropriate progression of the cell cycle, the CDKN2 gene has been investigated intensively as a new candidate tumor suppressor gene in many malignant tumors. Adult T-cell leukemia/lymphoma (ATLL) is a peripheral T-cell malignancy associated with human T-cell lymphotrophic virus type 1 (HTLV-1). As the development to ATL is believed to require not only HTLV-1 infection but also accumulation of genetic alterations, we investigated the relationship between alterations in the CDKN2 gene and ATL. Alterations in the CDKN2 gene were detected in approximately 15 to 20% of ATL patients. Interestingly, most of the patients with CDKN2 gene alterations had the aggressive form of ATL. The CDKN2 gene appears to be the major tumor suppressor gene on chromosome 9p21, and alteration in this gene may play an important role during late stages in the transformation process induced by HTLV-1. 相似文献
7.
Dimitrios Koutsimpelas Uwe Felmeden Wolf J. Mann Jürgen Brieger 《Journal of neuro-oncology》2011,103(3):437-443
Vestibular schwannomas (VS) are benign tumors of the nervous system that are usually sporadic but also occur in the inherited
disorder neurofibromatosis type 2 (NF2). The NF2 gene is a tumor suppressor gene located on chromosome 22. Loss of the NF2
protein product, Merlin, is universal in both sporadic and NF2-related schwannomas and the loss or mutation of the gene is
the only established causative event underlying schwannoma formation. Comparative genomic hybridization (CGH) was used to
screen 20 sporadic VS to identify additional chromosomal regions that may harbor genes involved in VS-tumorigenesis. The most
common change were losses on chromosome 22q. Additionally, losses were observed on chromosome 9p indicating a possible participation
of the CDKN2A tumor suppressor gene in the genesis of VS. Gains were observed on 17q, 19p and 19q, which have been reported
before in malignant peripheral nerve sheath tumors that are associated with neurofibromatosis type 1. Importantly, high level
amplifications have been observed on 16p and 16q as well as on 9q, suggesting the possible involvement of several oncogenes
in the tumorigenesis of VS. Our data suggest the involvement of various oncogenes and tumor suppressor genes might play a
role in the genesis of the vestibular schwannomas apart from the inactivation of the NF2 gene. 相似文献
8.
目的 探讨恶性外周神经鞘膜瘤(MPNST)中巢蛋白和细胞增殖核抗原Ki-67的表达情况及其意义。方法 采用免疫组织化学SP法检测42例MPNST中巢蛋白和Ki-67的蛋白表达。24例外周神经良性肿瘤作为对照。结果 MPNST中巢蛋白阳性表达率为95.2 %(40/42),其中巢蛋白强阳性表达率为40.5 %(17/42),明显高于外周神经良性肿瘤的4.2 %(1/24),差异有统计学意义(χ2=8.403,P=0.004);MPNST中Ki-67标记指数介于1 %~70 %之间,其中>3 %的占64.3 %(27/42),24例外周神经良性肿瘤中Ki-67标记指数均<3 %,两者比较差异有统计学意义(χ2=23.518,P=0.000)。结论 联合检测巢蛋白和Ki-67免疫标志物有助于MPNST的诊断。 相似文献
9.
Keng VW Rahrmann EP Watson AL Tschida BR Moertel CL Jessen WJ Rizvi TA Collins MH Ratner N Largaespada DA 《Cancer research》2012,72(13):3405-3413
The genetic evolution from a benign neurofibroma to a malignant sarcoma in patients with neurofibromatosis type 1 (NF1) syndrome remains unclear. Schwann cells and/or their precursor cells are believed to be the primary pathogenic cell in neurofibromas because they harbor biallelic neurofibromin 1 (NF1) gene mutations. However, the phosphatase and tensin homolog (Pten) and neurofibromatosis 1 (Nf1) genes recently were found to be comutated in high-grade peripheral nerve sheath tumors (PNST) in mice. In this study, we created transgenic mice that lack both Pten and Nf1 in Schwann cells and Schwann cell precursor cells to validate the role of these two genes in PNST formation in vivo. Haploinsufficiency or complete loss of Pten dramatically accelerated neurofibroma development and led to the development of higher grade PNSTs in the context of Nf1 loss. Pten dosage, together with Nf1 loss, was sufficient for the progression from low-grade to high-grade PNSTs. Genetic analysis of human malignant PNSTs (MPNST) also revealed downregulation of PTEN expression, suggesting that Pten-regulated pathways are major tumor-suppressive barriers to neurofibroma progression. Together, our findings establish a novel mouse model that can rapidly recapitulate the onset of human neurofibroma tumorigenesis and the progression to MPNSTs. 相似文献
10.
The neurofibromatoses represent two of the most common inherited tumor predisposition syndromes affecting the nervous system. Individuals with neurofibromatosis 1 (NF1) are prone to the development of astrocytomas and peripheral nerve sheath tumors whereas those affected with neurofibromatosis 2 (NF2) develop schwannomas and meningiomas. The development of traditional homozygous knockout mice has provided insights into the roles of the NF1 and NF2 genes during development and in differentiation, but has been less instructive regarding the contribution of NF1 and NF2 dysfunction to the pathogenesis of specific benign and malignant tumors. Recent progress employing novel mouse targeting strategies has begun to illuminate the roles of the NF1 and NF2 gene products in the molecular pathogenesis of NF-associated tumors. 相似文献
11.
Pascale?Lévy Dominique?Vidaud Karen?Leroy Ingrid?Laurendeau Janine?Wechsler Giulia?Bolasco Béatrice?Parfait Pierre?Wolkenstein Michel?Vidaud Ivan?Bièche
Background
Neurofibromatosis type 1 (NF1) is an autosomal dominant disorder with a complex range of clinical symptoms. The hallmark of NF1 is the onset of heterogeneous (dermal or plexiform) benign neurofibromas. Plexiform neurofibromas can give rise to malignant peripheral nerve sheath tumors (MPNSTs), and the underlying molecular mechanisms are largely unknown. 相似文献12.
13.
Kyriazoglou AI Dimitriadis E Arnogiannaki N Brandal P Heim S Pandis N 《Cancer genetics》2011,204(12):677-681
Secondary peripheral chondrosarcoma is a malignant chondroid tumor arising in a benign precursor, either an osteochondroma or an enchondroma. Multiple osteochondromas syndrome (MO) is an autosomal dominant skeletal disorder associated with bony growths in the form of osteochondromas that occasionally undergo malignant transformation to secondary peripheral chondrosarcomas. We describe the genetic examination of three secondary peripheral chondrosarcomas that had arisen synchronously from osteochondromas in a patient with MO by chromosome banding, high resolution chromosomal comparative genomic hybridization, and mutation analysis of the EXT1 and EXT2 genes. In two of the tumors (the third was not genetically informative), very similar chromosome abnormalities were found, indicating that they must somehow be part of the same neoplastic process in spite of being anatomically distinct. 相似文献
14.
Barnard ZR Agarwalla PK Jeyaretna DS Farrell CJ Gerstner ER Tian D Curry WT 《Journal of neuro-oncology》2011,104(2):605-610
We report the case of a seventy-five year old woman with a sporadic primary malignant intracerebral nerve sheath tumor (MINST).
These tumors fall within the spectrum of malignant peripheral nerve sheath tumors (MPNST) but confirming the diagnosis of
a MINST can be difficult due to its rarity and unusual intraparenchymal location. Radiographically, MINST’s mimic malignant
glioma and should be considered in the differential diagnosis of enhancing cerebral lesions. In this report, we present a
comprehensive panel of histological, immunohistochemical, ultrastructural, and genetic considerations that may be used to
diagnosis MINST based on their similarities to MPNSTs and brain parenchyma location. 相似文献
15.
Koyye RT Mahadevan A Santosh V Chickabasaviah YT Govindappa SS Hegde T Shankar SK 《Brain tumor pathology》2003,20(2):79-83
Cellular schwannomas rarely involve the cranial nerves, being more common in the spinal and peripheral nerves. A rare case
of cellular schwannoma involving the gasserian ganglion, a hitherto unreported site, that extended infratentorially to present
as a cerebellopontine angle tumor is reported. It is important to recognize that cellular schwannomas can histologically mimic
malignant peripheral nerve sheath tumors because of their high cellularity and mitotic activity, but they are relatively benign
tumors with a tendency to recur but not metastasize. 相似文献
16.
Maria Demestre Menderes Yusuf Terzi Victor Mautner Peter Vajkoczy Andreas Kurtz Ana Luisa Piña 《Journal of neuro-oncology》2013,115(3):391-399
Neurofibromatosis type 1 (NF1) is an inherited genetic disease affecting 1 in 3,500 individuals. A prominent feature of NF1 is the formation of benign tumours of the peripheral nerve sheath (neurofibromas). However, these can become malignant and form highly metastatic malignant peripheral nerve sheath tumours (MPNST), which are usually fatal despite aggressive surgery, chemotherapy, and radiotherapy. Recent studies have shown that pigment epithelium-derived factor (PEDF) can induce differentiation and inhibit angiogenesis in several kinds of tumours. The present study was designed to determine the in vitro and in vivo effects of PEDF on MPNST angiogenesis and tumour growth. PEDF inhibited proliferation and augmented apoptosis in S462 MPNST cells after 48 h of treatment in culture. In xenografts of S462 MPNST cells in athymic nude mice, PEDF suppressed MPNST tumour burden, due mainly to inhibition of angiogenesis. These results demonstrate for the first time inhibitory effects of PEDF on the growth of human MPNST via induction of anti-angiogenesis and apoptosis. Our results suggest that PEDF could be a novel approach for future therapeutic purposes against MPNST. 相似文献
17.
Summary Malignant peripheral nerve sheath tumors are rare spindle-cell sarcomas derived from Schwann cells or pluripotent cells of
the neural crest. They arise from the spinal roots, peripheral nerves, brachial and lumbosacral plexi, cranial nerves and
terminal nerve fibers within soft tissue, intestine, lung and bone. These tumors recur either locally, or metastasize distally.
Most of these tumors occur in association with neurofibromatosis type 1. Spinal cord metastasis from malignant nerve sheath
tumors associated with neurofibromatosis type 1 is very rare. We describe a rare case of near-total spinal cord metastasis
in a patient with malignant nerve sheath tumor in the absence of neurofibromatosis, and highlight the microscopic findings
and natural history of this disease process. 相似文献
18.
Hirokawa Y Nakajima H Hanemann CO Kurtz A Frahm S Mautner V Maruta H 《Cancer biology & therapy》2005,4(4):379-381
PAK1, a Rac/CDC42-dependent Ser/Thr kinase, is required for the malignant growth of RAS transformants as well as both NF1-deficient and NF2-deficient cancer cells. FK228, a histone deacetylase (HDAC) inhibitor, suppresses the growth of more than 70% of human cancers in vivo including RAS transformants, breast cancers and prostate cancers by activating a set of genes including the tumor suppressors gelsolin and p21(WAF1), that block upstream and downstream of PAK1, respectively. Here we demonstrate that (1) the anti-PAK1 drug FK228 (0.1 nM) completely blocks the growth of both NF1-deficient and NF2-deficient cancer cells in vitro, and that (2) FK228 (2.5 mg/kg, i.p., twice a week) causes the complete regression of an NF1-deficient human malignant peripheral nerve sheath tumor (MPNST) xenograft in nude mice. This is the very first case where a chemical drug in clinical trials for cancers has ever worked so effectively on neurofibromatosis (experimental neurofibromas) in vivo. 相似文献
19.
A malignant peripheral nerve sheath tumor in association with a paratesticular ganglioneuroma 总被引:1,自引:0,他引:1
A malignant peripheral nerve sheath (PNS) tumor and a benign ganglioneuroma were present as a composite tumor in the paratesticular area of a 15-year-old boy. The pathologic diagnosis was made by characteristic histologic and ultrastructural features and was supported by the demonstration of neuron-specific enolase (NSE)-positive ganglion cells and S-100-protein-positive spindle cells consistent with schwannian cells. The patient has remained disease-free for 3 years since the orchiectomy and the retroperitoneal lymph node dissection, which was followed by combination chemotherapy. 相似文献
20.
Defective mismatch repair (MMR) in humans causes hereditary nonpolyposis colorectal cancer. This genetic predisposition to colon cancer is linked to heterozygous familial mutations, and loss-of-heterozygosity is necessary for tumor development. In contrast, the rare cases with biallelic MMR mutations are juvenile patients with brain tumors, skin neurofibromas, and café-au-lait spots, resembling the neurofibromatosis syndrome. Many of them also display lymphomas and leukemias, which phenotypically resembles the frequent lymphoma development in mouse MMR knockouts. Here, we describe the identification and characterization of novel knockout mutants of the three major MMR genes, mlh1, msh2, and msh6, in zebrafish and show that they develop tumors at low frequencies. Predominantly, neurofibromas/malignant peripheral nerve sheath tumors were observed; however, a range of other tumor types was also observed. Our findings indicate that zebrafish mimic distinct features of the human disease and are complementary to mouse models. 相似文献