金蝉止痒胶囊抗过敏作用的实验研究

目的 研究金蝉止痒胶囊的抗过敏作用,为其临床应用提供实验依据.方法 采用2,4二硝基氯苯（2,4-Dinitrochlorobenzene,DNCB）致小鼠迟发性超敏反应模型、抗血清致大鼠同种被动皮肤过敏反应模型和抗血清致大鼠颅骨骨膜肥大细胞脱颗粒模型,分别观察金蝉止痒胶囊对小鼠超敏反应强度（耳肿胀度）、同种抗原攻击后皮肤伊文思蓝渗出抑制率和大鼠颅骨肥大细胞脱颗粒率的影响.结果 与模型对照组比较,金蝉止痒胶囊高、中、低剂量组对DNCB所致小鼠超敏反应强度（耳肿胀度）有显著抑制作用（P＜0.05）;对抗血清致大鼠同种被动皮肤过敏反应有显著拮抗作用（P＜0.05）;对抗血清所致大鼠颅骨骨膜肥大细胞脱颗粒率有显著抑制作用（P＜0.05）.结论 金蝉止痒胶囊具有良好的抗过敏作用.     

苦参对大鼠Ⅰ型变态反应的抑制作用

目的观察单味中药苦参对大鼠Ⅰ型变态反应的影响。方法以卵蛋白为变应原致敏大鼠,制备抗卵蛋白的抗血清(含IgE)。采用大鼠同种被动皮肤过敏反应(PCA)实验法,将抗血清注射到正常动物皮内,建立Ⅰ型变态反应的实验动物模型。将大鼠随机分为正常对照组(NS)、模型组(NS)、苦参高剂量(2.8×103 g/L)组、苦参低剂量(1.4×103 g/L)组、脱敏组方(2.8×103 g/L)组及酮替酚(0.4g/L)组,分别灌胃给药,然后以卵蛋白及伊文思蓝染料进行抗原攻击,测量蓝斑直径,采用分光光度计比色法测量致敏部位染料的渗出量。以抗卵蛋白血清被动致敏的大鼠颅骨骨膜肥大细胞为观察对象,从形态学角度观察苦参对肥大细胞脱颗粒的影响。结果给予高、低剂量的苦参均可抑制大鼠同种PCA,明显减少大鼠颅骨骨膜肥大细胞脱颗粒。高剂量苦参对致敏局部皮肤染料渗出和颅骨骨膜肥大细胞脱颗粒的抑制作用明显强于低剂量苦参(F=370.254、56.326,q=10.737、5.569,P0.05);高剂量苦参的抗Ⅰ型变态反应作用与酮替酚相似(q=2.820、1.313,P0.05),但次于脱敏组方(q=3.755、2.671,P0.05)。结论单味中药苦参具有抗Ⅰ型变态反应的作用,呈剂量依赖性。苦参的抗Ⅰ型变态反应作用次于中药复方制剂。     

内脏高敏感大鼠腹腔肥大细胞活性的改变及5-羟色胺对其的作用

目的：证实内脏高敏感大鼠腹腔中肥大细胞活性增加，探讨5－羟色胺（5－HT）影响肥大细胞脱颗粒的作用。方法：用腹腔注射鸡卵清白蛋白制备内脏高敏感大鼠，并随机分为A组和B组；空白对照鼠（腹腔注射生理盐水）随机分为C组和D组。提取内脏高敏感大鼠和对照组大鼠的腹腔肥大细胞，用抗原和抗血清孵育刺激肥大细胞脱颗粒，计数肥大细胞脱颗粒率，并用荧光法检测其组胺释放率。比较经不含5－HT孵育液组（A组和D组）和富含5－HT孵育液组（B组和C组）孵育的肥大细胞脱颗粒率和组胺释放率。结果：内脏致敏组大鼠（A组和B组）的组胺释放率明显高于D组大鼠（P＜0．01），A组和B组的脱颗粒率也显著高于D组（P＜0．01）；且A组的组胺释放率显著高于B组（P＜0．05），C组和D组间组胺释放率未见明显差异。结论：内脏致敏大鼠的肥大细胞活性增强，5－HT对致敏肥大细胞的脱颗粒功能有影响。     

葛根汤抗过敏药理作用的实验研究

本文运用抗过敏药理实验方法研究证明葛根汤能显著抑制小鼠耳异种ＰＣＡ及小鼠同种ＰＣＡ，阻止大鼠腹腔肥大细胞及颅骨骨膜肥大细胞脱颗粒，并能拮抗组胺对豚鼠离体回肠的收缩反应．     

腹腔注射卵清白蛋白致大鼠内脏高敏感的研究

目的：建立鸡卵清白蛋白致内脏高敏感大鼠模型，研究该模型内脏高敏感与肥大细胞的关联。方法：腹腔注射鸡卵清白蛋白使大鼠内脏致敏，分别在给药3d及2周后用特殊染色法观察结肠肥大细胞的形态学改变。用腹部撤离反射（abdominal withdrawal reflex,AWR）评估致敏大鼠对直肠扩张刺激的内脏感觉改变。结果：甲苯胺蓝染色法显示，致敏大鼠肠黏膜及肠系膜肥大细胞数量明显增加（P＜0．01）。阿尔辛蓝－藏红染色法显示，对照组及致敏大鼠3ml及5ml气囊组AWR评分显著高于对照组（P＜0．01）。结论：腹腔注射鸡卵清白蛋白致内脏高敏感的作用确切，内脏致敏作用很可能和肥大细胞的增生和脱颗粒状态相关。     

螺旋藻治疗大鼠变应性鼻炎的实验研究

目的:研究螺旋藻对大鼠实验性变应性鼻炎的治疗作用。方法：用卵清蛋白致敏大鼠制成变应性鼻炎动物模型,口服螺旋藻治疗,观察其行为学差异;用组织病理学方法观察鼻黏膜改变及肥大细胞脱颗粒;用酶联免疫吸附法（ELISA）检测血清总IgE水平;用荧光法检测血清组胺含量。结果：行为学得分,螺旋藻治疗组明显低于阴性对照组,差异有统计学意义（P<0.01）;鼻黏膜炎性反应明显轻于阴性对照组;鼻黏膜肥大细胞总数、脱颗粒数和血清总IgE、组胺含量明显低于阴性对照组（P<0.01）;螺旋藻治疗组、阳性对照组（强的松治疗组）和正常对照组之间差异无统计学意义（P>0.05）。结论：螺旋藻可防治大鼠变应性鼻炎的发生,提示螺旋藻可用于临床治疗变应性鼻炎。     

乙酸联合束缚应激对大鼠内脏敏感性及肠道肥大细胞状态的影响

目的：探讨腹泻型肠易激综合征动物模型的建立方法及模型评估。方法将30只成年SD大鼠随机分为乙酸刺激加束缚应激组（n＝10只）,束缚应激组（n＝10只）,和正常对照组（n＝10只）。采用乙酸刺激加束缚应激方法建立IBS动物模型,进行大便情况评估和直肠敏感性测试,盐酸甲苯胺蓝染色观察肠道肥大细胞数量及脱颗粒现象。结果在实验第7天,模型组大鼠粪便点数较正常组增多,差异有统计学意义（P＜0.05）;乙酸联合束缚应激组稀便数较其余两组增多,差异有统计学意义（P＜0.05）。乙酸联合束缚应激组大鼠直肠敏感性较其余两组增高,差异有统计学意义（P＜0.05）。乙酸联合束缚应激组大鼠肠道肥大细胞的数量较正常组增多,差异有统计学意义（P＜0.05）,乙酸联合束缚应激组大鼠肠道肥大细胞可见脱颗粒现象。组织学分析显示各组大鼠均无明显的炎症性表现。结论乙酸联合束缚应激可增加大鼠内脏敏感性,并且使大鼠肠道肥大细胞数量增多,细胞脱颗粒现象增强。该综合造模方法能够较好的模拟人类IBS的发病形式,为我们进一步研究IBS的发生发展机制提供了理想的动物模型基础。     

肠毒清颗粒对肝硬变内毒素血症大鼠模型内毒素及炎症因子的干预作用

目的：探讨肠毒清颗粒对大鼠肝硬变内毒素血症的干预作用。方法：70只大鼠分为正常对照组10只,余60只制作大鼠肝硬变模型组。模型制作成功后,分为模型组、肠毒清高剂量组、肠毒清低剂量组、乳果糖治疗组。观察各组大鼠的内毒素、TNF-α、IL-6及NO水平。结果：模型组与正常组比较,血浆内毒素、肝匀浆TNF-α、IL-6及NO水平明显升高（ P<0.05）;与模型组比较,肠毒清高剂量组、肠毒清低剂量组、乳果糖组大鼠血浆内毒素、肝匀浆TNF-α、IL-6及NO水平明显降低（ P<0.05）。肠毒清高剂量组与乳果糖组比较,内毒素、TNF-α、IL-6差异显著（ P<0.05）;与肠毒清低剂量组比较,血浆内毒素、IL-6差异有统计学意义。乳果糖组TNF-α、NO与肠毒清低剂量组比较,有显著性差异（ P<0.05）。结论：肠毒清颗粒能够防治肝硬变内毒素血症。     

新疆阿魏抗过敏的药理作用

本文报道了新疆阿魏挥发油水乳剂进行抗过敏药理实验研究。结果表明,本药给小鼠ip50mg/kg能显著抑制PCA反应。并能抑制大鼠颅骨骨膜肥大细胞的脱颗粒和兔的Archus反应。本药对致敏兔回肠因抗原攻     

参芍软肝汤抗大鼠肝纤维化的作用机制研究

目的 研究参芍软肝汤抗大鼠肝纤维化的作用及其机制.方法 选取Wistar雄性大鼠72只,正常喂养1周后分为对照组(n=12)和造模组(n=60),造模组采用40％四氯化碳(CCl4)橄榄油溶液腹腔皮下注射构建大鼠肝纤维化模型,造模成功后分为模型组(等量生理盐水灌胃)、阳性对照组(秋水仙碱0.154 mg·kg-1·d-1)、低剂量组(每100 g体质量2 mL,含参芍软肝汤生药0.23 g/mL)、中剂量组(每100 g体质量2 mL,含参芍软肝汤生药0.46 g/mL)及高剂量组(每100 g体质量2 mL,含参芍软肝汤生药0.69 g/mL)各12只,均治疗8周后观察大鼠的肝纤维化相关指标的差异.结果 对照组大鼠全部为0期标准,阳性对照组大鼠的肝组织纤维化程度显著优于模型组(P＜0.05),低剂量组、中剂量组、高剂量组的大鼠肝组织纤维化程度逐渐减轻,高剂量组大鼠肝组织纤维化程度显著轻于模型组和低剂量组(P＜0.05).模型组大鼠的Ⅲ型前胶原(PC-Ⅲ)、层粘连蛋白(LN)、Ⅳ-C型胶原(Ⅳ-C)、透明质酸(HA)检测值均显著高于对照组大鼠(P＜0.05),阳性对照组、低剂量组、中剂量组、高剂量组的PC-Ⅲ、LN、Ⅳ-C、HA检测值均显著低于模型组大鼠(P＜0.05).模型组大鼠的血清超氧化物歧化酶(SOD)检测值均显著低于对照组大鼠(P＜0.05),模型组大鼠的血清丙二醛(MDA)水平显著高于对照组大鼠(P＜0.05);阳性对照组、低剂量组、中剂量组、高剂量组的血清SOD检测值均显著高于模型组大鼠(P＜0.05),血清MDA水平显著低于模型组大鼠(P＜0.05).结论 参芍软肝汤能有效减轻大鼠肝纤维化程度,其作用机制为降低血清肝纤维化指标的水平,抑制过氧化物产生,减轻自由基对肝细胞的损伤作用.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.