尿液分析仪检测尿液白细胞的探讨

目的探讨尿液分析仪临床用于检测尿液白细胞的价值。方法采用罗氏Urisys 1800尿液分析仪、AVE-764B尿液有形成分分析仪和显微镜,随机对2011年9月至2012年1月的200例门诊患者尿样进行检测,比较三种方法检验结果的符合程度。结果显微镜镜检白细胞阳性率24%,AVE-764B尿液有形成分分析仪阳性率30%,二者联合检验出尿样阳性者52例;显微镜镜检与罗氏Urisys 1800尿液分析仪联合阳性率30.1%,二者联合检验出尿样阳性者54例;罗氏Urisys 1800尿液分析仪与AVE-764B尿液有形成分分析仪二者联合检验出尿样阳性者35例;罗氏Urisys 1800尿液分析仪、AVE-764B尿液有形成分分析仪和显微镜三者联合检测尿液白细胞阳性尿样仅19例。结论罗氏Urisys 1800尿液分析仪和AVE-764B尿液有形成分分析仪检测尿液白细胞虽然具有检测轻松、快捷等优点,但临床上并不能完全替代显微镜。     

两种方法检测尿液白细胞的比较探讨

目的 分析采取尿液分析仪及显微镜检查尿白细胞的结果.方法 选取进行尿液检验的患者200例进行临床观察,分别给予患者尿液分析仪及显微镜检查,分析两种方法的检测结果.结果 两种检测方法的结果存在着显著性差异,有统计学意义(P＜0 05) 结论 在尿液检查中,可以将尿液分析仪及显微镜检查这两种方法相结合,能够将尿液白细胞的假阴性率及假阳性率有效降低,提高检测结果的准确性     

尿液分析仪对比尿沉渣镜检法检测尿红、白细胞的分析与探讨

目的:探讨尿液分析仪对比尿沉渣镜检法检测尿红、白细胞的结果的差异,判定尿液分析仪在临床的应用价值。方法:分别采用本院iq2000尿液分析仪和尿沉渣镜检法对本院1325份尿液标本进行红细胞以及白细胞的检测,将两种不同检测方法尿液的红细胞以及白细胞的情况进行对比分析,以尿沉渣镜检为金标准来判定尿液分析仪的准确情况。结果:尿液分析仪组的红细胞真阳性为521例,真阴性为782例,灵敏度为95.9%,特异度为97.3%;尿液分析仪组的白细胞真阳性为569例,真阴性为738例,灵敏度为96.9%,特异度为97.6%。结论:尿液分析仪联合尿沉渣镜检法能够降低漏检率,提高检测的精准度,能够较好的为临床的诊断以及治疗提供有效的检测依据,临床上尿液分析仪能作为一个筛选试验检查。     

尿液分析仪检测尿中白细胞的几点看法

随着选进医疗设备在医学领域的使用，尿液常规检验由手工操作发展到目前的尿十项，这对临床有关疾病的诊断，治疗，疗效观察有重要意义。但笔者在工作中发现尿分析仪检测白细胞与尿沉渣镜检中的白细胞数量经常出现不一致的现象。为此，提出以下几点看法。     

全自动尿沉渣分析仪与显微镜检测尿液白细胞和红细胞的对比分析

目的：研究尿液白细胞（White Blood Cell,WBC）、红细胞（Red Blood Cell,RBC）检测中应用全自动尿沉渣分析仪检查与显微镜检查的效果。方法：研究对象为本院2021年9月～12月采集的500份晨尿样本,所有尿液样本均进行全自动尿沉渣分析仪与显微镜检测,观察比较两种检查方式的阳性检出情况及全自动尿沉渣分析仪检测尿液WBC、RBC的效能。结果：500份尿液样本中,显微镜检查WBC阳性检出率为14.60%,尿沉渣分析仪检查WBC阳性检出率为24.80%,真阳性率、假阴性率、假阳性率、真阴性率分别为12.20%、2.40%、12.60%、72.80%,以显微镜检查结果为金标准,则尿沉渣分析仪检测WBC的准确率、灵敏度、特异度分别为84.90%、83.45%、85.15%。500份尿液样本中,显微镜检查RBC阳性检出率为12.80%,尿沉渣分析仪检查RBC阳性检出率为19.80%,真阳性率、假阴性率、假阳性率、真阴性率分别为11.00%、1.80%、8.80%、78.40%,以显微镜检查结果为金标准,则尿沉渣分析仪检测RBC的准确率、灵敏度、特异度分别为89.40%、...     

URISCAN S-300型尿分析仪测定尿液红细胞和白细胞结果评价

近年来 ,用干化学法测定尿液红细胞、白细胞尿分析仪逐步在临床实验室普及。由于仪器型号不同 ,结果差异有时较大。为此 ,我们对ＵＲＩＳＣＡＮＳ- 30 0型尿分析仪测定尿液红细胞、白细胞结果进行评价 ,并与显微镜法进行比较。1　材料与方法1 1　材料　韩国盈东制药株式会社生产ＵＲＩＳＣＡＮＳ - 30 0型尿分析仪及其配套用尿试纸条 ,标本 70份 (正常人尿液 2 0份 ,泌尿系统疾病患者尿液 5 0份 )。1 2　方法　 (1)干化学法 :用校准试纸条校准仪器后 ,将试纸条浸入混匀尿液 1ｓ取出 ,在吸水纸上沾去多余尿液 ,置尿分析仪传送带上 ,仪器自动…     

尿液中红细胞,白细胞仪器检测与人工镜检对比

尿常规检验是诊断肾、泌尿系统和其它有关疾病的常用方法。尿液半自动分析仪的使用,提高了工作效率,弥补了人工容易漏检的项目。但有些因素的影响易造成假阳性及假阴性。经过仪器检测LEU、BLD与人工镜检白细胞、红细胞的结果比较,证明了尿液镜检与仪器检测的符合率。并提示出尿液镜检技术是尿常规检测中不可省略的一步。 一、材料与方法 选取本院1998年6月12日至9月26日期间的门诊病人200例。男86     

尿液分析仪和显微镜镜检法检测尿液WBC对比分析

目的探讨尿液分析仪和显微镜镜检法检测尿液白细胞(WBC)结果,找出检测方法。方法用尿液分析仪和显微镜镜检法同时对1894例尿液标本进行白细胞的检测。结果尿液分析仪检测白细胞结果为阴性者,镜检法检出白细胞超出正常范围(4个/HP)的占1.1%,而白细胞镜检范围在0~4个HP时(正常范围)尿液分析仪的结果呈阳性(±~+++)却占34.87%。结论尿液分析仪和显微镜镜检法测定尿液白细胞之间不成正比关系,尿液分析仪不能代替显微镜镜检法对尿液白细胞的检测。     

尿液干化学法检测与镜检相结合的重要性

目的:揭示尿液干化学检测与镜检结合的重要性.方法:采用尿液分析仪和人工显微镜法对600例标本同时检测,对其结果进行对比.结果:以人工显微镜检查为对照,干化学法红细胞(RBC)检测结果的阳性符合率为92.82％(194 /209),阴性符合率为86.96(340/391),假阳性率为13.04％(51/391),假阴性率为7.18％(15/209);白细胞(WBC)检测结果的阳性符合率为81.34％(218/268),阴性符合率为93.97％(312/332),假阳性率为6.02％(20/332),假阴性率为18.66％(50/268).结论:两种方法检测的结果有一定的差异,尿液分析仪检查结果不能完全替代显微镜检测法,应将两种方法结合起来综合分析.     

健康人群尿汞本底值的调查

目的探讨不同地区非接触汞正常人群尿汞正常值.方法在上海、黑龙江、广东、四川等省市,收集非接触汞正常人群尿样共2 248例.样本采集采用随意尿,统一使用聚乙烯塑料瓶收集不少于25 ml的尿样,统一使用上海光华仪器厂生产的F732-G型测汞仪,测定方法采用尿中汞的酸性氯化亚锡还原-冷原子吸收法和尿中汞的碱性氯化亚锡还原-冷原子吸收法.并用肌酐和比重校正.采用苦味酸法测定肌酐,采用比重计测定比重.结果尿汞测定值呈偏态分布,采用对数转换成正态或近似正态分布,用几何均数表示平均水平.酸性法和碱性法2种测定方法的比较,差异无统计学意义（P=0.099）.男女间各地区比较,差异无统计学意义（P＞0.05）.尿汞正常值95%上限为≤4.25μg/L（21.22 nmol/L）,用肌酐校正后为3.724 5 μg/g Cr（18.38 mol/g Cr）,比重校正后为4.45μg/L（22.50 nmol/L）.结论建议尿汞正常参考值为≤5μg/L（25 mol/L）,肌酐校正后为≤4 μg/L Cr（20nmol/Cr）,比重校正后为≤5 μg/L（25 nmol/L）.     

Handling of cytostatic drugs and urine mutagenesis

Summary As part of a French national epidemiologic study on human reproduction among hospital personnel, we investigated urine mutagenicity of nurses and personnel from oncology units exposed to cytostatic drugs. During a first series of experiments, urine mutagenicity of 47 subjects working in six oncology units was investigated in the Marseille regional's hospital. A control group of 37 individuals working in one cardiology clinic was also included. Urinary mutagens were extracted on XAD-2 resin and tested by two bacterial mutagenicity tests: the Ames test with tester strains Salmonella typhimurium TA 97, TA 98, TA 100 and TA 102 with or without metabolic activation (S9 MIX) and the SOS Chromotest with tester strain Escherichia coli PQ 37-S9 MIX. Bactericidal activity towards the tester strains was found in 40% of the urine samples (36/90). During a second series of experiments, urine mutagenicity of 17 office clerks was also investigated. Toxicity was found in six of the 21 urine samples. No significant difference of toxicity distribution and no relationship between toxicity and cigarette smoking were found. Qualitative analysis of the data showed no significant difference among the exposed groups and the control group (Chi 2 = 0.529, df = 2) with tester strain TA 98 + S9 MIX. Cigarette smoking was found to be the main factor of increased urinary mutagenicity (Chi 2 = 0.529, df = 1). Quantitative analysis of the data showed that mutagenic potencies varied from 0.332 ±0.539 revertants/mg creatinine to 7.226 ± 6.743 revertants/mg creatinine with TA 98 + S9 MIX. A relationship between the number of cigarettes smoked and mutagenic potency was found (Spearman rank coefficient r _s = 0.412, P < 0.05). One urine sample was found to be mutagenic with tester strain TA 102 and PQ 37.     

Influence of coffee on the excretion of noradrenaline and adrenaline in urine

Summary In field studies, the excretion rate of urinary catecholamines is very often used as an indicator of strain. Interfering effects which are due to caffeine, for example, can only be quantified if the influence of coffee consumption on the excretion of catecholamines is known quantitatively. This was the aim of our study with five subjects, on five consecutive working days, and with a strict standardization of nutrition. The urine samples were specified with respect to the following parameters: sampling period, volume, urine status, density, creatinine, noradrenaline, and adrenaline. Adrenaline showed a significant correlation with coffee consumption, whereas noradrenaline did not. Moreover, it could be demonstrated that relating the concentration of catecholamines to the creatinine excretion is insufficient for work physiology studies, especially if the urine sampling periods are as short as 2h.This study was supported by the Deutsche Forschungsgemeinschaft, Bad Godesberg (Ru 240/5-1)     

The level of nickel in smoker's blood and urine

General population is exposed to nickel from various sources. Smoking presents a significant form of exposure. The research was conducted in period 2000--2003 in Institute of Public Health in Nis. The samples of tobacco and cigarettes (127 samples) were both domestic and imported, and samples of biological material (123 blood samples and 147 urine samples) were taken from occupationally unexposed persons (smokers and non-smokers). The analyses were performed by electrothermal atomization technique, by Perkin Elmer AAS M-1100. The results obtained, revealed a high content of nickel in cigarettes (2.32-4.20 mg/kg) and in tobacco (2.20-4.91 mg/kg) regardless of the kind and the origin of tobacco. Nickel content in the blood of smokers (0.01-0.42 microg/l, median 0.07 microg/l) was higher than in the blood of non-smokers (0.01-0.26 microg/l, median 0.06 microg/l) although this difference was not statistically significant (p>0.05). In the urine of smokers (<0.01-8.20 microg/l, median 1.20 microg/l) there was a significantly higher concentration of nickel than in the urine of non-smokers (<0.01-4.60 microg/l, median 0.50 microg/l), p<0.05. The exposure of smokers to nickel through tobacco smoke was high regardless of the kind and the origin of tobacco and cigarettes. The content of nickel in tissue fluids established by biomonitoring shows that smokers can be far more exposed to this carcinogenic substance than non-smokers and that health risks for smokers are higher in this context.     

Vitamin supplementation reduces the level of homocysteine in the urine of autistic children

Significant differences in homocysteine levels in the urine of autistic children are observed. We hypothesized that vitamin supplementation might reduce the level of urinary homocysteine. To rationalize such a hypothesis, analyses were performed using the gas chromatography/mass spectrometry method. The homocysteine level in the urine of autistic children was measured twice: (1) before vitamin supplementation (group C, 30 autistic children) and (2) after supplementation, with either folic acid and vitamins B₆ and B₁₂ (group A1, 24 autistic children) or vitamins B₆ and B₁₂ alone (group A2, 6 autistic children). The homocysteine level in the urine of autistic children before vitamin supplementation was 2.41 ± 1.10 mmol/mol creatinine (mean ± SD difference). After treatment, the homocysteine level was reduced to 1.13 ± 0.44 and 1.33 ± 0.39 mmol/mol creatinine for A1 and A2 groups, respectively. The intake of vitamins B₆ and B₁₂, together with folic acid, was found to be more effective in lowering the levels of urinary homocysteine than the intake of vitamins B₆ and B₁₂ alone. Our findings may lead to the recommendation of including vitamins B₆ and B₁₂ together with folic acid supplementation in the diets of children with autism.