兔足重度冻伤后红细胞免疫粘附功能的动态变化

目的 通过测定红细胞膜Ｃ３ｂ受体的活性和红细胞膜吸附的免疫复合物,揭示冻伤对红细胞免疫吸附的作用。方法 对兔足重度冻伤后,采用红细胞膜Ｃ３ｂ受体花环法和红细胞免疫复合物花环法进行检测。连续观察冻伤后红细胞受体功能的动态变化。结果 冻伤早期红细胞膜Ｃ３ｂ受体花环率比正常明显下降（Ｐ〈０．０１）,后逐渐恢复。红细胞免疫复合物花环率显著提高（Ｐ〈０．０１）,并且一直高于正常。结论 重度冻伤后红细胞膜的免     

胃溃疡患者红细胞免疫功能变化的临床意义

目的 :探讨胃溃疡患者的红细胞免疫功能状态。方法 :应用免疫粘附法对 45例胃溃疡患者红细胞免疫活性进行检测并与正常组进行比较。结果 :胃溃疡患者红细胞C3b受体花环率 (RBC C3bRR :19.97± 4.2 9)、肿瘤红细胞花环率 (RBC TRR :3 4.5 8± 6.45 )均明显低于正常对照组 ( 2 5 .3 6± 5 .67和 43 .82± 6.73 ) (P <0 .0 1)。红细胞免疫复合物花环率 (RBC ICR)在胃溃疡组 ( 6.95± 2 .5 6)显著高于对照组 ( 5 .3 5± 1.98) (P <0 .0 1)。结论 :胃溃疡患者红细胞免疫功能呈低下状态。红细胞免疫可能通过微循环的改变参与了胃溃疡的发生和发展     

老年人红细胞免疫功能检测及黄芪对其的调节作用

研究表明,老年人红细胞C_3b受体花环率和红细胞免疫复合物花环率均明显降低,红细胞免疫粘连抑制因子活性升高,而增强因子活性则下降,循环免疫复合物含量明显上升,C_3水平显著下降,各指标与青年人相比,差异显著(P<0.001)。服用黄芪后,老年人红细胞免疫功能得到明显的加强,上述指标均获得明显改善,与用药前相比,有显著差异(P<0.001)。     

血清对红细胞免疫粘附抑制作用的测定

本文报道测定血清红细胞免疫粘附抑制活性的新方法。在系统性红斑狼疮病人组,血清红细胞C_(3b)受体花环形成抑制率(55.7±38 3％)高于正常人组(21.40±12.30％ P<0.01)。结果表明红细胞C_(3b)受体花环形成抑制率试验是简单和可重复的。对血清红细胞C_(3b)受体花环形成抑制率测定机理与临床意义也进行了讨论。     

红细胞免疫及其调节功能测定方法

红细胞免疫是一新的研究领域。1982年至今,我室已建立了10种红细胞免疫及其调节功能测定方法:测定红细胞膜C_3b受体活性与粘附免疫复合物含量的红细胞C_3b受体花环、红细胞免疫复合物花环;红细胞混合花环;测定血清中红细胞免疫粘附抑制因子和促进因子的红细胞C_3b受体花环抑制试验     

SLE患者红细胞C3b受体与E花环的关系

<正> SLE患者红细胞免疫粘附功能与循环免疫复合物的关系国外学者研究较多,但对SLE患者红细胞免疫粘附功能与淋巴细胞免疫功能变化关系的研究不多。我们对28例SLE患者红细胞C3b受体花环率与E花环率、活性花环率变化的关系进行     

高原红细胞增多症患者红细胞流变性与红细胞SOD、血浆MDA关系的研究

目的:研究不同海拔高度高原红细胞增多症患者红细胞流变学特性与脂质过氧化的关系。方法:对不同海拔高度(刚察县3300m,甘德县4080m)高原红细胞增多症患者检测了红细胞滤过指数(EFI)、红细胞免疫功能、红细胞超氧化物歧化酶(SOD)和血浆丙二醛(MDA)水平。结果:随着海拔的升高,EFI、红细胞免疫复合物花环率和血浆MDA水平上升,而红细胞C₃b受体花环率和红细胞SOD随之降低;相关分析表明:红细胞SOD与EFI和红细胞免疫复合物花环率呈负相关(P＜0.01);而与红细胞C₃b受体花环率呈正相关;MDA则相反。结论:随着海拔升高,高原红细胞增多症患者机体脂质过氧化反应增强,导致了红细胞变形能力降低。     

SLE患者红细胞免疫与T淋巴细胞免疫功能变化的初步观察

SLE患者红细胞免疫粘附功能与循环免疫复合物的关系国外学者研究较多,但对SLE患者红细胞免疫粘附功能与T淋巴细胞免疫功能变化关系的研究不多。我们对28例SL正患者红细胞C3b受体花环率与E花环率、活性花环率变化的关系进行了对比研究。发现红细胞C3b受体花环率与活性花坏绝对值之间有正相关关系,现将资料报道如下。     

正常家兔红细胞免疫粘附活性的昼夜节律变化

我们按照祖国医学天人相应和一天阴阳消长的四个时辰(子、午、卯、酉)观察了日本大耳白兔(11只,为同群体封闭型)红细胞免疫粘附活性的昼夜节律变化,结果:①红细胞Cab受体酵母菌花环形成率,最高峰值为卯时(6.5％),最低谷值为子时(2.7％),相着显著(P<0.05)。②红细胞免疫复合物花环形成率最高峰值为酉时(12.27％),     

血液病患者红细胞免疫粘附功能的变化

观察了四种常见血液病共88例患儿红细胞C3b受体花环率(RCR)和红细胞免疫复合物花环率(RICR)的变化情况,并与30例健康儿童进行比较,同时观察RCR和RICR在血液病病情变化的动态规律;结果发现,红细胞C3b受体花环率在急性白血病为9.44％±1.27％、再生障碍性贫血为9.60％±1.30％、原发性血小板减少性紫癜为10.27％±1.39％,均明显低于正常儿童(13.87％±2.91％);过敏性紫癜患儿RCR19.77％±1.42％显著高于正常对照(P<0.01),红细     

日本血吸虫肾病动物实验的研究

本文报道了急性感染日本血吸虫家兔肾病的免疫病理研究。用酶标抗体间接法在10只感染兔中5只的肾脏切片上显示出血吸虫抗原定位,并用 IHA、ELISA 测到肾匀浆中的特异性抗原和抗体,与肾脏大体标本及光镜下所见病理改变相一致,提示日本血吸虫肾病可能由免疫复合物致病。进一步用肾脏中特异性抗原组分作酶标定位分析,表明有虫卵抗原、成虫表膜相关抗原、肠相关抗原,以及特异性 IgG 参与肾脏内免疫复合物形成。     

日本血吸虫成虫抗原和虫卵抗原检测先天性感染仔兔血清抗体的动态变化

目的　观察仔兔血清抗体动态变化 ,探讨家兔日本血吸虫病垂直传播的体液免疫反应规律。方法　 5只怀孕晚期母兔分别人工感染 5 0 0条日本血吸虫尾蚴 只 ,仔兔出生后 4 3d起每隔 2周采血收集血清 ,至仔兔发育成熟 (约出生后113d)。分别运用日本血吸虫成虫抗原 (AWA)和可溶性虫卵抗原 (SEA) ,ELISA法检测血清中特异性IgG、IgM抗体 ,观察动态变化。结果　仔兔先天性感染率为 6 0 % (12 2 0 ) ,其中 5只双性感染 ,7只单性感染。 2 0只仔兔 ,抗AWA IgG、抗AWA IgM抗体检测始终呈阴性 ;抗SEA IgG检测 ,5只双性感染仔兔有 4只于出生后 5 7d起陆续呈阳性 ,其余 16只始终为阴性 ;2 0只仔兔抗SEA IgM也始终呈阴性。结论　先天性感染日本血吸虫病的仔兔呈低免疫应答状态 ,可能存在免疫耐受     

REACTIVITY OF LYMPHOCYTE SUBPOPULATIONS IN HUMAN MIXED LYMPHOCYTE CULTURE

Loss of antigenicity in the mixed lymphocyte culture (MLC) reaction of lymphocytes precultured at 22°C for 7–10 days was accompanied by a decrease in bone-marrow-derived lymphocytes (B cells) from 22 ± 1% to 13 ± 1%, and an increase in thymus-derived lymphocytes (T cells) from 65 ± 2% to 83 ± 1% (P < 0.001). Depletion of B cells from a fresh lymphocyte suspension by either antihuman immunoglobulin-coated column fractionation or by sheep red blood cell (SRBC) rosette formation resulted in a significant reduction of the cell's ability to stimulate in MLC (P < 0.001). Coating of lymphocytes with rabbit antihuman brain serum abrogated their ability to respond but not the ability to stimulate in MLC.     

脑出血后患者红细胞免疫功能和血液流变学指标测定

目的 :探讨发生脑出血后的红细胞免疫粘附功能和血液流变性的变化。方法 :对 40例脑出血患者的红细胞免疫粘附功能 (RCIA)和血液流变学 11项指标进行分析。结果 :表明脑出血患者RBC C3 bRR显著低于对照组 (P <0 .0 0 1) ,而RBC ICR显著高于对照组(P <0 .0 0 1) ,全血粘度低切高于对照组 (P <0 .0 1) ,血浆粘度、血沉、血沉方程K值、纤维蛋白原、甘油三酯均明显高于对照组 (P <0 .0 0 1)。结论 :脑出血的发病与RCIA功能紊乱和血液流变性异常有密切相关关系。     

Enumeration of Rosette Forming Cells in Man—A Comparison of Three Methods

A comparison of three methods for the enumeration of rosette forming cells in man was done with simultaneous staining for surface immunoglobulins for the identification of B-cells. The three methods were as follows: incubation of the lymphocytes with (1) sheep red cells and 25% fetal calf serum (E-rosettes), (2) sheep red blood cells and 25% horse serum (E-rosettes), and (3) neuraminidase treated sheep red blood cells and 25% fetal calf serum (E_N-rosettes). The percentage of E_N-rosettes (76.4% ± 3.3%, mean ± S.D.) was significantly higher than the percentage of E_H-rosettes (70.4% ± 3.6%), and E-rosettes (62.1% ± 4.1%) (P < 0.001). The percentage of B-cells were not significantly different from each other under all three conditions, and less than 1% of the immunoglobulins bearing cells could form rosettes.     

IL-17 neutralization significantly ameliorates hepatic granulomatous inflammation and liver damage in Schistosoma japonicum infected mice

IL-17 is a signature cytokine of Th17 cells implicated in the induction and progression of chronic inflammatory diseases. Several studies in C57BL/6 mice, immunized with soluble schistosome egg Ags (SEA) in complete Freund's adjuvant (CFA), and subsequently infected with Schistosoma mansoni (S. mansoni) have shown that severe hepatic granulomatous inflammation is correlated with high levels of IL-17. Here, using a Schistosoma japonicum (S. japonicum) larvae infection model in C57BL/6 mice, we analyzed the dynamic expression of IL-17 in infected livers by RT-qPCR and ELISA. Our results showed that IL-17 expression was elevated during the course of infection. The temporal expression of IL-17 and cytokines/chemokines involved in the induction and effector function of Th17 cells was paralleled with hepatic granulomatous inflammation. Treatment of S. japonicum infected mice with IL-17-neutralizing mAb resulted in significant downmodulation of granulomatous inflammation and hepatocyte necrosis. The protection was associated with lower expression of proinflammatory cytokines/chemokines, such as IL-6, IL-1β, CXCL1, and CXCL2 and a reduced number of infiltrating neutrophils. Anti-IL-17 mAb significantly ameliorated hepatic granulomatous inflammation, partly through the downregulation of proinflammatory cytokines/chemokines and recruitment of neutrophils. Our data indicate a pathogenic role of Th17/IL-17 in hepatic immunopathology in S. japonicum infected mice.     

The role of immunoconglutinin in the in vivo and in vitro destruction of red cells

Two experimental approaches were used to investigate the role of immunoconglutinin (IK) in the in vivo destruction of red cells in rabbits. In a first series of experiments the behaviour of EC43 (C3-coated red cells) was followed in IK-producing rabbits and in rabbits passively receiving IK, both of which had previously been complement-depleted by cobra venom factor (CoF). The red cells were sequestered to a minor degree and returned to the circulation within 20–30 min, whereas in the normal control, EC43 returned to the circulation over a period of 3–4 hr. In contrast to EC43, EC43IK (IK-coated EC43) did not form rosettes around the Kupffer cells, suggesting that IK blocks the functional activity of C3 and so interferes with the interaction between C3 and C3-receptors on fixed macrophages. In a second series of experiments EC43 and EC43IK, injected into IK-producing rabbits and normal rabbits respectively, underwent marked lysis and erythrophagocytosis. Examination of liver imprint preparations from these rabbits revealed rosette formation around the Kupffer cells, indicating the fixation of more C4 and C3 by bound IK.

In vitro experiments confirmed both the inhibitory activity and the complement-fixing capacity of IK.

The results suggest that IK normally has an amplifying effect on complement fixation in vivo and so potentiates the ability of complement to bring about red-cell destruction.

     

日本血吸虫完整成虫盐水浸液抗原的化学及免疫学特性

本文报告日本血吸虫完整成虫盐水浸液抗原(ASE)的化学、免疫化学及免疫学性质。实验结果表明ASE中含有蛋白质、糖和类似核酸的物质。它与成虫抗原有相同的抗原成分与虫卵抗原也有共同的决定簇。PAGE结果显示ASE与新鲜成虫经Triton处理后的可溶性部份比较类似.以抗ASE与成虫冰冻切片进行IFA试验的结果证明ASE主要定位于虫的表膜,可以认为ASE主要含有成虫的表膜抗原。本实验中无论用ASE免疫家兔或用抗ASE-γ-G被动免疫小鼠都未能诱导动物产生对攻击感染的抵抗力;但抗ASE在有补体的参与下可在体外促使白细胞粘附在童虫的表面,产生对童虫的攻击作用。     

从基因水平对IL-5与IL-10在日本血吸虫感染小鼠中作用的探讨

本研究对日本血吸虫感染小鼠脾细胞体外在SEA与ConA诱导下产生的IL-5和IL-102种细胞因子从mRNA转录水平进行研究,以探索这2种细胞因子mRNA转录水平的动态变化与肉芽肿形成与调节的可能性.日本血吸虫尾蚴感染雄性BALB/C小鼠后,分别于感染后3周、5周、8周、10周和12周取小鼠脾脏,制备脾细胞单细胞悬液并于含ConA或SEA的培养基中培养后提取脾细胞总RNA.RT-PCR及凝胶分析法被用来分析总RNA样品中IL-5及IL-10mRNA转录水平.研究结果显示,未感染和感染3周小鼠脾细胞均无IL-5和IL-10的表达,感染后第5周的小鼠脾细胞出现IL-5特异性条带,感染后第8周IL-5表达明显增强,感染后第10周IL-5mRNA转录水平下降,感染后第12周未能检测到IL-5表达,表明IL-5表达的动态变化与肉芽肿的形成、发展及调节相平行.IL-10mRNA转录也于感染后第5周出现,但感染后第8周、第10周、第12周与第5周相比,IL-10的表达水平并无明显改变,显示IL-10mRNA转录水平并未随着肉芽肿的调节而变化.本研究结果提示IL-5可能在肉芽肿形成和调节过程中可能起重要作用;而IL-10可能并未直接参与肉芽肿的调节,但其转录可能与防止宿主过度肉芽肿炎症反应有关.     

Allotypic determinants on the surface of rosette-forming cells in the rabbit

Antigen-binding cells to sheep erythrocytes from non-injected rabbits and from rabbits after a single injection of sheep red blood cells (SRBC) have been detected by the immunocyto-adherence technique (rosette formation). The expression of immunoglobulin allotypic determinants on these antigen-binding cells has been investigated by inhibition of rosette formation with anti-allotype antisera. The results indicate that a and b locus allotypes are expressed by the great majority of rosette-forming cells (RFC) in the lymph nodes of injected and non-injected rabbits. Ms3 allotype is expressed by practically all RFC from non-injected rabbits, while, after injection of SRBC, a population of RFC appears, which does not express this allotype. An antiserum to d 11 consistently failed to inhibit RFC. Pretreatment of lymphocytes from rabbits heterozygous at the b locus with antiserum to only one allelic product produced an inhibition of rosette formation which was approximately half that obtained when antiglobulin reagents to both allelic products were added. The results suggest that in heterozygous animals, the two allelic markers are expressed on two different populations of antigen-binding cells. Electron microscopy studies on the morphology of RFC showed that the majority of the RFC were formed around small lymphocytes.