NK细胞和IL-2分泌细胞在新生儿脐血中的表达

在机体自身免疫调节中NK细胞和IL 2分泌细胞起重要作用 ,因此 ,研究NK细胞和IL 2分泌细胞的表达 ,将有助于我们了解机体细胞免疫的功能 ,新生儿细胞免疫不成熟 ,是其易于感染的重要原因 ,有关于新生儿脐血细胞免疫功能的研究 ,国内有些报道[1,2 ] ,但NK细胞和IL 2分泌细胞方面的研究甚少 ,本文将新生儿NK细胞和IL 2分泌细胞检测结果报道如下 :1　材料与方法1 1　检测对象　随机选择我院产科母孕期健康足月顺产新生儿 70例作为脐血组 ,其中男 4 0例 ,女 30例 ,平均胎龄 39周 ,平均出生体重 30 5 0g ,出生阿氏评分≥ 9分 ,体检正常 ,其…     

患哮喘产妇的新生儿脐血IL-8测定

支气管哮喘是一种慢性气道变应性炎症(AAI),其基本特征是气道高反应性(AHR),其发生与炎症细胞的浸润、激活及所释放的细胞因子和介质有关. 近年来,炎症介质和炎症细胞在AHR发生中的作用逐渐引起人们的重视.大量研究表明,气道的中性粒细胞与AH-R的产生关系密切[1,2];IL-8是中性粒细胞特异性趋化因子和活化剂,已有报道认为它与AHR的产生有关[3,4].但有关IL-8在哮喘发病中的作用了解较少. 本研究选用从未直接与外界变应原接触的患哮喘产妇的新生儿脐血中的IL-8进行测定,以期发现它与哮喘的关系.     

脐血单个核细胞表达IL-12、IFN-γ及其mRNA的观察

白细胞介素１２（ＩＬ－１２）的主要作用是促进ＴＨ１细胞和自然杀伤细胞（ＮＫ）产生γ－干扰素（ＩＦＮ－γ）,介导细胞免疫。脐血单个核细胞（ＣＢＭＣ）产生ＩＬ－１２的能力鲜见报道。重组ＩＬ－１２（ｒＩＬ－１２）在体外能促进新生儿ＣＤ４＋Ｔ细胞产生ＩＦＮ－...     

TF口服液诱导T细胞IL-2、IL-6及CD分子的表达

转移因子 (ｔｒａｎｓｆｅｒｆａｃｔｏｒ,ＴＦ)是介导细胞免疫的重要因子之一 ,其ＴＦ口服液具有协同诱导人Ｔ淋巴细胞ＩＦＮ γ和ＨＬＡ ＤＲ/ＤＱ分子的表达[1] ,促进小鼠腹腔巨噬细胞吞噬鸡红细胞的吞噬功能 ,促进人Ｔ淋巴细胞增殖发挥良好的免疫调节作用[2 ] 。为进一步了     

激素对T淋巴细胞生长激素基因表达调控的影响

目的 :探讨各种激素对T淋巴细胞GH基因表达的影响。方法 :构建含人GH调控序列的荧光素酶报告基因质粒PGL2 GH luc,然后转染入T淋巴细胞系———JurkatE6 1细胞中 ,在培养液中分别加入各种激素。结果 :各浓度的GH、GHRH对Jurkat细胞中荧光素酶的表达有抑制作用 (P <0 0 1) ,而高剂量的TRH( 10、10 0nmol/L)和SS( 10 0nmol/L)对Jurkat细胞中荧光素酶的表达也有抑制作用 ( P<0 0 5 )。结论 :T淋巴细胞中GH基因的表达受下丘脑激素和GH的调节。     

T细胞激活的分子机制

Ｔ细胞激活是一个多种分子相互作用的复杂过程，这一过程持续２～３周，涉及１００多个基因的表达。本文就ＩＬ－２的表达及其基因调控在Ｔ细胞激活中的作用及癌基因和抑癌基因在Ｔ细胞激活过程中的分子机制予以归纳介绍。     

IL-2和IL-15协同调节T细胞的增殖和LAK细胞的杀伤活性

目的 探讨IL-2和IL-15在免疫调控和应答中的协同作用。方法 IL-2、IL-15和IL-2 IL-l5组刺激CTLL细胞的增殖，^3H—TdR掺入法测cpm值；IL-2、IL-15和IL-2 IL-l5组诱导PBMC中NK和LAK细胞的发育，4h^51Cr释放实验检测对K562和LiBr细胞的杀伤活性。结果 IL-2和IL-15都能诱导CTLL认细胞的增殖和NK、LAK细胞的杀伤活性，IL-2和IL-15可明显协同上调CTLL认的增殖和LAK细胞的杀伤活性。结论 IL-2和IL-15在免疫调控和应答中有一定的协同作用，为细胞因子联合应用于临床治疗肿瘤等疾病提供了实验依据。     

新生儿脐血T细胞IL—2R表达和血清sIL—2R水平的研究

对新生儿脐血Ｔ细胞ＩＬ－２Ｒ表达率和血清ｓＩＬ－２Ｒ水平进行检测，结果显示：（１）早产儿脐血Ｔ细胞ＩＬ－２Ｒ自然表达率明显低于足月新生儿正常儿童组，而后两者间无差异；（２）经ＰＨＡ活化后足月新生儿早产儿Ｔ细胞ＩＬ－２Ｒ表达率皆显著低于正常儿童组，早产儿组也明显低于足月新生儿组；（３）足月新生儿和早产儿脐血血清ｓＩＬ－２Ｒ水平均明显高于正常儿童组，但前两者间无明显差异，（４）新生儿组（包括足月和早产     

1542例新生儿脐血TSH,T4联检结果及应用价值探讨

血清TSH、T4水平是诊断原发性和继发性甲状腺机能减退的可靠、灵敏、特异性指标。脐血纸片法TSH、T4主要用于新生儿先天性甲状腺机能减退的筛选。我们应用脐带血纸片法对1542例TSH(IRMA)、T4(RIA)进行联检,并探讨脐血纸片法对TSH、T4测定筛选新生儿甲减和新生儿缺碘临床应用价值,现将结果报告如下。     

新生儿脐血单核细胞在脂多糖刺激下分泌IL-6及出现IL-6mRNA表达的实验研究

目的 :通过观察LPS对新生儿脐血单个核细胞 (MC)分泌IL 6及表达IL 6mRNA基因的影响 ,探讨严重细菌感染时新生儿机体防御反应机制。方法 :取肝素抗凝剂脐血 ,用密度离心分离法分离MNC ,以RPMI16 40培养液调整细胞浓度为1× 10 6 ml- 1 ,将细胞悬液铺于 2 4孔培养板上 ,依次加入不同浓度脂多糖 (LPS)培养 36h或同一浓度LPS(1μg ml)培养不同时间 ,收集培养上清液及细胞 ,分别用ELISA和RT PCR方法测定IL 6及IL 6mRNA表达情况。结果 :①脐血MNC在LPS刺激 3、6、12、18、2 4、36h后IL 6分泌水平逐步增高 ,6h以后增加尤为明显 ,与其他各时间点比较有非常显著的差异 (P <0 0 0 1)。LPS刺激组与无LPS对照组相同时间点比较 ,6h内IL 6变化水平无差异 ,6h以上各点有显著差异 (P <0 0 1)。RT PCR方法检测显示LPS刺激后 3h即可见IL 6mRNA基因表达。②脐血MNC受不同浓度LPS刺激时 ,IL 6分泌水平随LPS浓度递增。③全部脐血MNC均检测到IL 6mRNA基因表达。结论 :LPS能诱导新生儿脐血MNCIL 6mRNA基因转录 ,从而促使IL 6合成、分泌 ,该作用呈时间、剂量依赖性变化。     

C3 and C4 gene expression and interferon-gamma-mediated regulation in human glomerular mesangial cells.

The glomerular mesangial cell (GMC) plays a key role in the maintenance of glomerular structure and function and in the mediation of glomerular injury. To explore the potential of this cell to produce complement and react to local inflammatory signals, we studied the synthesis and regulation of the third and fourth components of complement in cultured human GMC. Using metabolic labelling and immunoprecipitation, we found that C3 and C4 polypeptide chains were synthesized and secreted by GMC. Interferon-gamma (IFN-gamma) led to an increase in C4 protein synthesis, but not C3 synthesis. There was a corresponding increase in C4 mRNA in IFN-gamma-activated cells, but no increase in C3 mRNA, as determined by semi-quantitative polymerase chain reaction (PCR) estimation. These results demonstrate that human GMC can synthesize C3 and C4 proteins, and that regulation of expression of the C4 gene is mediated by IFN-gamma. We hypothesize that GMC production of complement could influence the clearance of immune aggregates by the kidney and the mediation of glomerular injury.     

Interleukin-4 and interferon-gamma production by peripheral blood mononuclear cells from food-allergic patients

The aim of this study was to investigate whether patients with food allergy had a cytokine imbalance of interleukin (IL)-4 and interferon-gamma (IFN-γ) production. Diagnostic procedures including skin prick tests, determination of food-specific serum IgEs, and positive double-blind, placebo-controlled, food challenges identified 15 adult patients. They were compared with 15 age- and sex-matched healthy subjects. Peripheral blood mononuclear cells (PBMC) were incubated for 24, 48, and 72 h in the presence of phytohemagglutinin plus phorbol myristate acetate. After mitogen stimulation, culture supernatants from patients with food allergy contained significantly less IFN-γ but increased IL-4 when compared with healthy controls. Secretion of IL-4 was maximal at 24 h and IFN-γ secretion was maximal at 72 h. There was no correlation between cytokine secretion in vitro and serum IgE level. These findings demonstrated that an imbalance of IL-4 and IFN-γ production is present in food allergy, as documented in other allergic diseases, but other mechanisms are probably also involved.     

Interleukin-7 specifically induces the B7/BB1 antigen on human cord blood and peripheral blood T cells and T cell clones

B7/BB1 is a cell surface molecule and member of the Ig superfamily that is constitutively expressed on dendritic cells.In addition, B7 is expressed on B cells, macrophages, T cells,and T cell clones following activation. Interaction of B7 withits natural ligand CD28 is required for optimal stimulationof T cells, activated via the TCR-CD3 complex, which is thoughtto be due to stabilization of cytokine mRNA. Here we demonstratethat the expression of B7 on T cells can specifically be inducedby IL-7. Induction of B7 expression on T cells and T cell clonesrequires at least 5 – 7 days of culture and representsa late activation event. Results of studies using T cell clones,as well as resting purified B7^– T cells, demonstrate thatB7 is induced on a substantial proportion of T cells after IL-7activation and is not due to an outgrowth of pre-existing B7+T cells. In addition, CD4+ as well as CD8+ T cells could beinduced to express B7. Stimulation of purified cord blood Tcells with cross-linked anti-CD3 mAb resulted in a relativelyfast (48 h) induction of B7, which could not be inhibited bya neutralizing anti-IL-7 mAb, whereas no endogenous IL-7 productionby activated T cells and T cell clones could be detected. Together,these results indicate that the B7 molecule can be induced onT cells by IL-7, but also by an IL-7 independent pathway involvingtriggering of the TCR-CD3 complex.     

Interleukin-4 gene expression in human peripheral blood mononuclear cells

Interleukin-4 (IL-4) mRNA was detected in normal human peripheral blood mononuclear cells (PBMC) stimulated with concanavalin A by Northern blot analysis. The signal was undetectable in PBMC before the stimulation, but became detectable 3 hrs after the stimulation and reached a maximum in 3-6 h and disappeared gradually thereafter. Immunosuppressive drugs such as ciclosporin, hydrocortisone and prednisolone inhibited the IL-4 mRNA expression dose dependently. Interferon-gamma did not show any inhibitory effect on IL-4 gene expression.     

Effects of interleukin-4 and interferon-γ on the secretion of IgG4 from human peripheral blood mononuclear cells

Whereas IgE antibodies are linked with allergy, IgG4 antibodies may reflect the state of immunity and protection against a particular antigen. It has been shown that interleukin (IL)-4 is required for induction of IgE synthesis. In order to elucidate the role of IL-4 in the production of IgG4 and to compare IgG4 and IgE regulatory processes, we quantified these immunoglobulin isotypes after in vitro culture of peripheral blood mononuclear cells (PBMC) in the presence of IL-4. The production of IgG4 was increased by IL-4 under the same conditions which are optimal for IgE production but not among PBMC from all donors, depending on the magnitude of spontaneous IgG4 secretion: IL-4 was effective only when the spontaneous secretion of IgG4 was < 7% of the total IgG secretion; it had no effect when spontaneous IgG4 production was >7% of total IgG. The IL-4-induced IgE response was consistently obtained when IgG4 was < 7% of total IgG but was markedly diminished or absent when IgG4 was > 7% of total IgG. If Staphylococcus aureus strain Cowan 1 (SAC) was present during the 48-h preincubation step, spontaneous IgG4 production was increased, but the stimulatory effect of this mitogen on immunoglobulin production, including IgG4, was markedly blocked by the addition of IL-4. In contrast, IL-4-induced IgE synthesis was strongly blocked by the presence of SAC. Finally, secretion of IgG4 (spontaneous and IL-4-induced) was suppressed among cells from most donors by interferon-γ (IFN-γ). These results suggest that IL-4 has opposite effects on in vitro IgG4 production and that the in vitro synthesis of both IgG4 and IgE appears to be regulated similarly by IL-4 and IFN-γ, whereas additional signals promote the production of one isotype in preference to the other. It is possible that activated B cells respond to IL-4 less well than do nonactivated cells.     

Interleukin-4 and Interleukin-4 Receptor Alpha Gene Polymorphisms in Recurrent Aphthous Stomatitis

ABSTRACT

Background: Recurrent aphthous stomatitis (RAS) is a common oral condition with a major impact on the quality of life. The condition is thought to be due to the overexpression of T helper-1(Th1)-related cytokines. Since interleukin-4 (IL-4) and its receptor (IL-4Rα) are antagonistic to Th-1 pathways, polymorphisms in their genes may also be involved in the pathogenesis of aphthous stomatitis.

Methods: Sixty-four patients diagnosed with minor RAS and 141 (age- and sex-matched) healthy controls were assessed for 3 single-nucleotide polymorphisms (SNPs) within the promoter region of the IL-4 gene (?1098G/T, ?590C/T, and ?33C/T), and 1 SNP in IL-4Rα gene (+1902 A/G).

Results: No significant differences were detected between the patient and the control group regarding IL-4 allele frequencies. However, the patient group demonstrated a higher frequency of IL-4 ?590 CC genotype and a lower rate of IL-4 ?590 TC genotype.

The TCT, GTT, GCT, and GTC haplotypes of the IL-4 gene (?1098, ?590, ?33) were significantly more frequent in the patients and the GCC, and TTT haplotypes were more common in healthy controls. No significant differences were found in IL-4Rα gene polymorphism between the 2 groups.

Conclusions: Certain polymorphisms of IL4 gene could predispose individuals to RAS.