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宋玉霞 《中国现代应用药学》2018,35(11):1694-1697
目的 对单面针根茎中的化学成分进行研究。方法 采用硅胶柱层析和制备液相色谱法进行分离纯化,并根据波谱数据对单体化合物进行结构鉴定。结果 从单面针根茎的乙醇提取物中分离纯化得到10个化合物,分别鉴定为:原阿片碱(1)、别隐品碱(2)、四氢小檗碱(3)、白屈菜红碱(4)、二氢血根碱(5)、二氢白屈菜红碱(6)、6-丙酮基二氢血根碱(7)、对羟基苯甲醛(8)、异香草醛(9)、丁香醛(10)。结论 10个化合物均首次在单面针中分离得到。 相似文献
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目的 研究糙叶败酱乙酸乙酯部位的化学成分。方法 利用硅胶及Sephadex LH-20凝胶柱色谱对其化学成分进行分离、纯化,根据理化性质及波谱数据进行结构鉴定。结果 从糙叶败酱水提取物的乙酸乙酯萃取部位分离得到11个化合物,分别为7α-甲氧基莫罗苷(1)、7β-甲氧基莫罗苷(2)、1-辛醇(3)、2,3-壬烯烃(4)、lariciresinol(5)、Jatamanin J(6)、落叶松脂醇-4-O-β-D-葡萄糖苷(7)、黄花败酱醇(8)、Jatamanin A(9)、Scabroside A(10)、十六烷酸-α-单甘油酯(11)。结论 化合物1~4为首次从该植物中分离得到,其中化合物3,4为首次从败酱科败酱属植物中分离得到。 相似文献
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目的 研究沉香Aquilaria sinensis的倍半萜类化学成分。方法 采用硅胶、ODS、Sephadex LH-20等多种柱色谱及制备型高效液相色谱等现代分离技术对沉香的化学成分进行分离纯化,并根据其理化性质、MS、1D和2D NMR等波谱方法进行结构鉴定;采用96孔板微量稀释法测试了分得的单体化合物对耐药金黄色葡萄球菌的抑菌活性。结果 从沉香95%乙醇提取物中分离得到7个倍半萜类化合物,分别鉴定为(+)-4a,5-二甲基-3-(丙-2-烯基)-八氢萘-2β,8a-二醇(1)、白木香酸(2)、白木香醇(3)、vetaspira-2(11),6-dien-14-al(4)、白木香醛(5)、(-)-10-表-γ-桉叶醇(6)、9β-羟基-α-沉香呋喃(7)。其中化合物1对耐甲氧西林金黄色葡萄球菌的最小抑菌浓度(MIC)为210 μmol/L。结论 化合物1为新的倍半萜类化合物,命名为2β,8aα-二羟基-11-烯-荒漠木烷,其对耐甲氧西林金黄色葡萄球菌有较好的抑制作用。 相似文献
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目的 研究三桠苦[Evodia lepta(Spreng.)Merr.]枝叶的化学成分,为三桠苦的进一步研究奠定物质基础。方法 对三桠苦无水乙醇提取物的乙酸乙酯萃取物采用硅胶柱层析分离纯化,得到单体化合物,经波谱分析鉴定其化合物结构。结果 从三桠苦乙酸乙酯萃取物中分离得到6个单体化合物,分别为异吴茱萸酮酚(化合物1)、异吴茱萸酮酚甲醚(化合物2)、3,5-二羟基-4-乙氧基-6-乙酰基-7-甲氧基-2,2-二甲基苯并二氢吡喃(化合物3)、(cis)-3,4,5-三羟基-6-乙酰基-7-甲氧基-2,2-二甲基色烷(化合物4)和(trans)-3,4-二羟基-5-甲氧基-6-乙酰基-7-甲氧基-2,2-二甲基色烷(化合物5)和对羟基苯甲酸丁酯(化合物6)。结论 化合物1~6均为首次从三桠苦植物的枝叶中分离得到。 相似文献
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摘 要 目的: 探讨秋海棠不同溶剂提取物的抗炎作用及其机制研究。方法: 制备秋海棠醇提取物、石油醚提取物、乙酸乙酯提取物、正丁醇提取物、水提取物,以 脂多糖(LPS)诱导RAW 264.7巨噬细胞活化,不同溶剂提取物作用24 h后,ELISA法检测上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素1β(IL-1β)的含量,Griess法测定一氧化氮(NO)的含量。 结果: 秋海棠乙酸乙酯提取物、正丁醇提取物能极显著抑制TNF-α、IL-1β、NO的含量(P<0.01),秋海棠醇提取物、乙酸乙酯提取物能极显著抑制NO的含量(P<0.01)。 结论:秋海棠醇提取物、乙酸乙酯提取物、正丁醇提取物抗炎作用显著,其机制可能与减少炎症因子的释放有关。 相似文献
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从羊蹄乙醇提取物分离鉴定了2个萘类化合物、5个蒽醌类化合物及其他4个化合物。萘类为2-甲氧基-6-乙酰基-7-甲基胡桃醌(1)、3-乙酰基-2-甲基-1,4,5-三羟基-2,3-环氧萘醌醇(2)、蒽醌类为大黄素甲醚(3)、大黄素(4)、大黄酚-8-O-β-D-葡萄糖苷(5)、大黄素-8-O-β-D-吡喃葡萄糖苷(6)、大黄酚(7),以及壬酸十五醇酯(8)、β-胡萝卜苷(9)、5-甲氧基-7-羟基-1(3H)-苯骈呋喃酮(10)和没食子酸(11)。化合物1、8~10为首次从羊蹄中分离得到,1、8为首次从酸模属植物中分离得到;体外细胞试验表明,化合物1对人肝癌HepG-2细胞、子宫癌Hela细胞、肺癌A549细胞具有较强的抑制作用,其IC50分别为2、1.8、4.6 μmol·L-1,化合物2对人肝癌细胞HepG-2的IC50为100 μmol·L-1。 相似文献
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摘 要 目的: 对甘肃榆中和碌曲的藏药刺柏叶中挥发油化学成分进行比较分析。 方法: 采用水蒸气蒸馏法提取挥发油,利用GC MS联用技术对其进行分离分析。结果:甘肃榆中和碌曲的得油率分别为0.21%和1.45%,两个地区刺柏叶中前者挥发油共分离出40个峰,鉴定出38个化合物,占分离物质的95%,后者挥发油共分离出51个峰,鉴定出47个化合物,占分离物质的92%。甘肃榆中刺柏叶中挥发油的主要成分为α-蒎烯(44.92%),1-石竹烯(9.23%)、(-)-异喇叭烯(6.50%)、α-石竹烯(5.60%)、月桂烯(4.54%)、d-杜松烯(3.37%);碌曲刺柏叶中挥发油的主要成分为二-非手性-α-柏木烯(31.87%)、环己烯(15.28%)、γ-榄香烯(10.05%)、澳白檀醇(5.80%)、α-蒎烯(5.79%)。结论: 两者刺柏叶挥发油成分有所不同,其得率及主要成分都有较大差异。该方法对建立刺柏质量标准控制有参考价值。 相似文献
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New 2,6-piperidinediones 2a–g and 4a–d were prepared by initial condensation of aromatic aldehydes or cycloalkanones with cyanoacetamide to give α-cyanocinnamides
la–g or cycloalkylidenes 3a,b which underwent Michae1 addition with ethyl cyanoacetate or diethylmalonate. Compounds 4a–d were alkylated by various alkyl halides to produce the N-alkylated 2,6-piperidinedione derivatives 5a–m.
Some new selected compounds 2a–c,f, 4a–d & 5e,h,j were pharmacologically evaluated for potential anticonvulsant, sedative and analgesic activities. These compounds exhibited
significant anticonvulsant and analgesic effects after a single I.P. administration 100 mg/kg b.wt. . On the other hand all
the investigated compounds induced hypnotic activity and prolonged the phenobarbital sodium- induced sleep as compared with
the control group and the most potent compound was found to be 2f. 相似文献
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EFFECT OF POLICOSANOL SUCCESSIVE DOSE INCREASES ON PLATELET AGGREGATION IN HEALTHY VOLUNTEERS 《Pharmacological research》1996,34(5-6)
Policosanol is a cholesterol-lowering drug with hypocholesterolemic effects demonstrated in experimental models, healthy volunteers and type II hypercholesterolemic patients. In addition, antiplatelet effects of policosanol have been shown in experimental models and healthy volunteers. The effect of successively increasing doses of policosanol on platelet aggregation was investigated in a randomized, placebo-controlled, double-blind study conducted in 37 healthy volunteers. The volunteers were on a placebo-baseline period (two tablets per day) for 7 days and thereafter they received randomly, under double-blind conditions, placebo or policosanol (10mgday−1) for 7 days. After this period dosage was doubled to 20mgday−1for the next 7 days and then again doubled to 40mgday−1, while the control group received placebo tablets all the time. Platelet aggregation as well as coagulation time was measured at baseline and after each dosing step. Results showed that antiplatelet effects of policosanol were successfully enhanced throughout the study, thus suggesting a dose-dependent relationship. No significant effect was reached during the first dosing period, but significant reductions of epinephrine and ADP-induced platelet aggregation were observed after the second one. Finally, a significant inhibition of platelet aggregation induced by all the agonists was observed at the last dosing step. Coagulation time remained unchanged during the trial. 相似文献
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NEURAMIDE STIMULATES ADRENOCORTICOTROPIN BUT NOT PROLACTIN RELEASE FROM RAT PITUITARY 《Pharmacological research》1996,34(5-6)
Neuramide (NMD), a substance found in crude preparations of porcine stomach extract, is a viral inhibitor that also has putative immunostimulatory effects. The effects of NMD on stress-hormone (ACTH and prolactin—PRL) release were assessed inin vivoandin vitrostudies. In the former, blood levels of corticosterone and PRL were measured in NMD-treated male rats.In vitroexperiments were performed to evaluate the effects of NMD and three of its fractions (obtained with high performance liquid chromatography) on ACTH and PRL release from perfused rat pituitary slices. NMD increased plasma corticosterone levelsin vivoand produced dose-dependent increases inin vitropituitary release of ACTH. No effects on PRL secretion were observedin vivoorin vitro. The stimulatory effects on ACTH release were caused by the NMD fraction with a molecular weight of >5000<10000Da. 相似文献
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In this study, the antibiotic susceptibilities to tigecycline and tetracycline of 35 selected Bacteroides fragilis group strains were determined by Etest, and the presence of tetQ, tetX, tetX1 and ermF genes was investigated by polymerase chain reaction (PCR). tetQ was detected in all 12 B. fragilis group isolates (100%) exhibiting elevated tigecycline minimum inhibitory concentrations (MICs) (≥8 μg/mL) as well as the 8 strains (100%) with a tigecycline MIC of 4 μg/mL, whilst tetX and tetX1 were present in 15% and 75% of these strains, respectively. All of these strains were fully resistant to tetracycline (MIC ≥ 16 μg/mL). On the other hand, amongst the group of strains with tigecycline MICs < 4 μg/mL (15 isolates), tetQ, tetX and tetX1 were found less frequently (73.3%, 13.3% and 46.7%, respectively). All but two strains harbouring the tetQ gene in this group were non-susceptible to tetracycline, with a MIC > 4 μg/mL. These data suggest that in most cases tigecycline overcomes the tetracycline resistance mechanisms frequently observed in Bacteroides strains. However, the presence of tetX and tetX1 genes in some of the strains exhibiting elevated MICs for tigecycline draws attention to the possible development and spread of resistance to this antibiotic agent amongst Bacteroides strains. The common occurrence of ermF, tetX, tetX1 and tetQ genes together predicted the presence of the CTnDOT-like Bacteroides conjugative transposon in this collection of Bacteroides strains. 相似文献
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《Pharmacological research》1996,34(5-6)
Inhibitory effects of the class III antiarrhythmic compound
/
-sotalol on acetylcholinesterase (AChE; EC 3.1.1.7) isoenzymes of both erythrocytes and the human caudate nucleus and on serum cholinesterase (ChE; EC 3.1.1.8) were studiedin vitrousing a spectrophotometric kinetic assay with acetylthiocholine (ASCh) as substrate. Sotalol concentrations in the assays varied from 0.32 to 3.2m
. All isoenzymes studied were inhibited by
/
-sotalol in a reversible and concentration-dependent manner. Double reciprocal plots of the reaction velocity against varying ASCh concentrations revealed that
/
-sotalol reduced substrate affinity (apparent Michaelis constant, KM, increased) of serum ChE, but did not change the enzyme's maximal rate of ASCh hydrolysis (Vmax). Thus,
/
-sotalol inhibition of serum ChE was of the competitive type (rate constant for reversible competitive inhibition: Ki=0.51m
). In contrast,
/
sotalol reduced the maximal reaction velocity of the AChE isoenzyme from the central nervous system (caudate nucleus), but had no influence on substrate affinity of the enzyme (KMwith ASCh unchanged) indicating purely non-competitive inhibition kinetics (rate constant of reversible non-competitive inhibition: Ki′=0.44m
).
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-sotalol inhibition of erythrocyte AChE was of mixed competitive/non-competitive type (Ki=0.31m
, Ki′=0.49m
). Non-competitive
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-sotalol inhibition of caudate nucleus AChE and the non-competitive component of erythrocyte AChE inhibition cannot be overcome by increased concentrations of the cholinergic transmitter acetylcholine (ACh). Peak
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-sotalol plasma levels as described in the literature for both humans (15μ
) and experimental animals (dogs: 18μ
; rats: 260μ
) as well as maximal myocardial concentrations of the substance (dogs: 46μ
; rats: 478μ
) are in the range of about 2% to 100% of the sotalol inhibition rate constants determined in the present paper for cholinesterase isoenzymesin vitro. Thus,
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-sotalol inhibition of ACh hydrolysisin vivomay contribute to both the well known antiarrhythmic potential and proarrhythmic side effects of the compound. 相似文献
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喙果黑面神化学成分研究 总被引:2,自引:0,他引:2
目的研究大戟科植物喙果黑面神(Breynia rostrata Merr.)的化学成分。方法利用硅胶、凝胶等色谱技术分离纯化化学成分,根据化合物的理化性质和光谱数据进行结构鉴定。结果从喙果黑面神的正丁醇萃取部分分离得到4个化合物,分别鉴定为6-O-甲基丙酰基-α-D-吡喃葡糖(6-O-methylpropanoyl-α-D-glucopyranose,1);4″-苯酚基-6-O-甲基丙酰基-β-D-吡喃葡糖苷(4″-phenolic-6-O-methylpropanoyl-β-D-glucopyranoside,2);1-O-没食子酰基-β-D-吡喃葡糖苷(1-O-galloyl-β-D-glucopyranoside,3);熊果苷(arbutin,4)。结论化合物1和2为新化合物,3和4均为首次从该种植物分离得到。 相似文献
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EFFECTS OF -GUANIDINE--METHYLQUINAZOLINE ON GASTRIC ACID SECRETION IN RATS 《Pharmacological research》1996,34(5-6)
In this study 2-guanidine-4-methylquinazoline (2-GMQ) appeared to decrease basal and stimulated gastric acid secretion, while structurally related compounds as dimethyl- biguanide, cyanoguanidine and 2-cyanoamino-4-methylpyrymidine did not. Thus, there is an antisecretory effect when the biguanide group is associated with a lipophilic structure. The antisecretive effects exerted by 2-GMQ are associated with anti H2-histamine activity.The anti H2-histamine nature of the effects of 2-GMQ was confirmed by the capacity of this compound of depressing the chronotropic activity of the isolated guinea pig auricle increased by histamine, as well as relaxant activity in rat uterus contracted by histamine, since both preparations are rich in H2-histamine receptors. 相似文献
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AMELIORIATION OF CHEMICALLY-INDUCED HEPATOCYTE INJURY BY CYCLOSPORINE A 《Pharmacological research》1996,34(5-6)
Cyclosporine A, beside its current applications, possesses potential hepatoprotective effects. This study was directed to investigate the effect of Cyclosporine A pretreatment on hepatic injury due to carbon tetrachloride (CCl4) and
-galactosamine. Rats were injected by two successive doses of Cyclosporine A (5mgkg−1day−1). Six hours after the second dose, 1mlkg−1of CCl4was administered i.p. Effects associated with Cyclosporine A pretreatment were examined by using isolated hepatocytes and hepatocytes that were immobilized and continuously perfused.
-Galactosamine (5m
) was added directly to the perfusion medium. After isolation, hepatocytes were examined histologically by light and electron microscopy, immobilized and perfused for further metabolic functional activity evaluation. Cyclosporine A pretreatmentin vivoproduced hepatoameliorative effects of various degrees which were statistically significant as manifested by: (1) an increased trypan blue exclusion after CCl4; (2) an improved ureagenesis after CCl4; (3) a reduction in the lipid droplets accumulation in the cytoplasm produced by CCl4administration; (4) well preserved cytoplasmic organelles as mitochondria, endoplasmic reticulum ER, nuclear chromatin structures that were altered by CCl4; and (5) an increased hepatocytes survival in the agarose gel matrix, reduction of LD leakage and improvement of ureagenesis after
-galactosamine addition to the perfusion medium. The beneficial effect of Cyclosporine A pretreatment in modifying hepatotoxicity of chemical insults merits further studies. 相似文献